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The map label for this gene is eno

Identifier: 190570657

GI number: 190570657

Start: 212224

End: 213498

Strand: Reverse

Name: eno

Synonym: WPa_0205

Alternate gene names: 190570657

Gene position: 213498-212224 (Counterclockwise)

Preceding gene: 190570658

Following gene: 190570656

Centisome position: 14.4

GC content: 37.49

Gene sequence:

>1275_bases
ATGATGAAAAAGACGATCAGCAGTGTATTTGCAAGAGAAATTTTAGATAGCAGGGGTTACCCAACAATTGAGGTAGAAAT
AGAACTATGTGATGGAGCAACAGGTAGAGCCGCTGTCCCTTCTGGGGCTTCGACCGGTAAACTAGAAGCATTGGAGCTGA
GAGATCAAGATGAAAAAAGGTATTGTGGTAAAGGAGTGCTGAAAGCTGTTCAATCTGTGAGTGGAGTGATAGCAAATAAA
ATCATTGGCATGGATGCAGCAAACCAGAGTGCAATTGATAAAGTTTTAATTGAACTAGATGGAACAAAAAATAAATCTAA
ACTTGGAGCAAATGCAACTTTGGGTGTGTCTCTTGCTGTTGCAAAAGCAGCAGCAAACAGTTTCAAAACACCGTTATATA
AATATTTGGGCGTAGGGGAGAAGCAGATGCCAGTTCCGCTAATTAACGTCATTAATGGTGGAGTACATGCGGATAATAAA
CTCGATTTTCAAGAATTTATGATTCTTCCGGTTGGTGCTGAAACTTTCAGTGAAGCAATAAGAATATCTGCAGAGGTATT
TCATAACTTGCGCAGCATTCTTAAGAAAAAAGGTTATAGTACAAACGTAGGAGATGAAGGTGGTTTTGCACCAAACATTG
GAAGCACTGAAGAAGCTCTTAATTTGATAATTCAGGCTGTTGAATCTGCCGGTTATTCGATGAAAAATCACTTTGCACTG
GGTCTTGATGTTGCTGCATCTACTTTTTATGAAGATGGAGTTTATAAATTTGAAAACAGAGAACTCACTTCAGAGGAATT
GGTTCAGTATTATTTTGACCTTGTAGGCAAATATCCAATAATTTCTATAGAAGATGCAATGAATGAAGACGACTATGAAG
GTTGGAAATTACTTACTGTAAAACTTGGGAATAAAGTTCAATTGGTTGGAGATGATTTATTTGTTACAAATTGTAAACTA
ATAAGTAAAGGAATAGAGGAGAAAATGGCAAATGCTGTACTAATCAAGCCAAATCAGATAGGAACACTTACGGAAACTTT
TGCTGCTATTGAAATGGCAAAATTAAATGGCTACAGGGCTATTATTTCGCATCGCTCAGGTGAAACAGAAGATACAACGA
TATCCCACATAGCGGTTGCATCGAATTGTGGACAAATCAAGACTGGCTCGCTGTCGCGTTCTGATAGGCTTGCAAAGTAT
AATGAATTAATGAGAATAGAAAGTTCATTAGGAGAAAATGCTAAATATTATTGTGGGTTAGCATGGACTTCATAG

Upstream 100 bases:

>100_bases
AATTACCTGTATATTGCAATGTTCGTACACTGAAATGTTACAGCGCTGGGGTGACATTTCCCGAACGTGAACACAATTGT
GAATCTAGTGGAGGTTTAGC

Downstream 100 bases:

>100_bases
ACGAAGTTAAATTATATTTAAAAGCAGGTGATGGTGGCGATGGCTGCGCAAGTTTTCGTCGAGAAAAATTCGTTGAATTT
GGTGGCCCAAACGGTGGTAA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MMKKTISSVFAREILDSRGYPTIEVEIELCDGATGRAAVPSGASTGKLEALELRDQDEKRYCGKGVLKAVQSVSGVIANK
IIGMDAANQSAIDKVLIELDGTKNKSKLGANATLGVSLAVAKAAANSFKTPLYKYLGVGEKQMPVPLINVINGGVHADNK
LDFQEFMILPVGAETFSEAIRISAEVFHNLRSILKKKGYSTNVGDEGGFAPNIGSTEEALNLIIQAVESAGYSMKNHFAL
GLDVAASTFYEDGVYKFENRELTSEELVQYYFDLVGKYPIISIEDAMNEDDYEGWKLLTVKLGNKVQLVGDDLFVTNCKL
ISKGIEEKMANAVLIKPNQIGTLTETFAAIEMAKLNGYRAIISHRSGETEDTTISHIAVASNCGQIKTGSLSRSDRLAKY
NELMRIESSLGENAKYYCGLAWTS

Sequences:

>Translated_424_residues
MMKKTISSVFAREILDSRGYPTIEVEIELCDGATGRAAVPSGASTGKLEALELRDQDEKRYCGKGVLKAVQSVSGVIANK
IIGMDAANQSAIDKVLIELDGTKNKSKLGANATLGVSLAVAKAAANSFKTPLYKYLGVGEKQMPVPLINVINGGVHADNK
LDFQEFMILPVGAETFSEAIRISAEVFHNLRSILKKKGYSTNVGDEGGFAPNIGSTEEALNLIIQAVESAGYSMKNHFAL
GLDVAASTFYEDGVYKFENRELTSEELVQYYFDLVGKYPIISIEDAMNEDDYEGWKLLTVKLGNKVQLVGDDLFVTNCKL
ISKGIEEKMANAVLIKPNQIGTLTETFAAIEMAKLNGYRAIISHRSGETEDTTISHIAVASNCGQIKTGSLSRSDRLAKY
NELMRIESSLGENAKYYCGLAWTS
>Mature_424_residues
MMKKTISSVFAREILDSRGYPTIEVEIELCDGATGRAAVPSGASTGKLEALELRDQDEKRYCGKGVLKAVQSVSGVIANK
IIGMDAANQSAIDKVLIELDGTKNKSKLGANATLGVSLAVAKAAANSFKTPLYKYLGVGEKQMPVPLINVINGGVHADNK
LDFQEFMILPVGAETFSEAIRISAEVFHNLRSILKKKGYSTNVGDEGGFAPNIGSTEEALNLIIQAVESAGYSMKNHFAL
GLDVAASTFYEDGVYKFENRELTSEELVQYYFDLVGKYPIISIEDAMNEDDYEGWKLLTVKLGNKVQLVGDDLFVTNCKL
ISKGIEEKMANAVLIKPNQIGTLTETFAAIEMAKLNGYRAIISHRSGETEDTTISHIAVASNCGQIKTGSLSRSDRLAKY
NELMRIESSLGENAKYYCGLAWTS

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the cell surface

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=427, Percent_Identity=52.2248243559719, Blast_Score=426, Evalue=1e-119,
Organism=Homo sapiens, GI301897477, Length=426, Percent_Identity=51.1737089201878, Blast_Score=411, Evalue=1e-115,
Organism=Homo sapiens, GI301897469, Length=426, Percent_Identity=51.1737089201878, Blast_Score=411, Evalue=1e-115,
Organism=Homo sapiens, GI4503571, Length=427, Percent_Identity=51.0538641686183, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI301897479, Length=424, Percent_Identity=47.1698113207547, Blast_Score=358, Evalue=7e-99,
Organism=Homo sapiens, GI169201331, Length=342, Percent_Identity=25.4385964912281, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI169201757, Length=342, Percent_Identity=25.4385964912281, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI239744207, Length=342, Percent_Identity=25.4385964912281, Blast_Score=94, Evalue=2e-19,
Organism=Escherichia coli, GI1789141, Length=419, Percent_Identity=57.9952267303103, Blast_Score=478, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=50.1160092807425, Blast_Score=399, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17536383, Length=428, Percent_Identity=50, Blast_Score=398, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI32563855, Length=211, Percent_Identity=42.654028436019, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6321693, Length=429, Percent_Identity=50.1165501165501, Blast_Score=392, Evalue=1e-110,
Organism=Saccharomyces cerevisiae, GI6323985, Length=433, Percent_Identity=49.6535796766744, Blast_Score=382, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324974, Length=433, Percent_Identity=49.4226327944573, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324969, Length=433, Percent_Identity=49.4226327944573, Blast_Score=380, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321968, Length=429, Percent_Identity=49.8834498834499, Blast_Score=369, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24580918, Length=428, Percent_Identity=51.4018691588785, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580916, Length=428, Percent_Identity=51.4018691588785, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580920, Length=428, Percent_Identity=51.4018691588785, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580914, Length=428, Percent_Identity=51.4018691588785, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI281360527, Length=428, Percent_Identity=51.4018691588785, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI17137654, Length=428, Percent_Identity=51.4018691588785, Blast_Score=389, Evalue=1e-108,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_WOLPP (B3CNP3)

Other databases:

- EMBL:   AM999887
- RefSeq:   YP_001975015.1
- ProteinModelPortal:   B3CNP3
- EnsemblBacteria:   EBWOLT00000000724
- GeneID:   6385627
- GenomeReviews:   AM999887_GR
- KEGG:   wpi:WPa_0205
- GeneTree:   EBGT00050000031016
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46006; Mature: 46006

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 206-206 ACT_SITE 336-336 BINDING 156-156 BINDING 165-165 BINDING 284-284 BINDING 311-311 BINDING 336-336 BINDING 387-387

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMKKTISSVFAREILDSRGYPTIEVEIELCDGATGRAAVPSGASTGKLEALELRDQDEKR
CCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCHHHH
YCGKGVLKAVQSVSGVIANKIIGMDAANQSAIDKVLIELDGTKNKSKLGANATLGVSLAV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEEEEECCCCCCHHCCCCCEEHHHHHH
AKAAANSFKTPLYKYLGVGEKQMPVPLINVINGGVHADNKLDFQEFMILPVGAETFSEAI
HHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEEEECHHHHHHHH
RISAEVFHNLRSILKKKGYSTNVGDEGGFAPNIGSTEEALNLIIQAVESAGYSMKNHFAL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEE
GLDVAASTFYEDGVYKFENRELTSEELVQYYFDLVGKYPIISIEDAMNEDDYEGWKLLTV
EHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCEEEEEE
KLGNKVQLVGDDLFVTNCKLISKGIEEKMANAVLIKPNQIGTLTETFAAIEMAKLNGYRA
EECCEEEEEECCEEEEHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHCCEEE
IISHRSGETEDTTISHIAVASNCGQIKTGSLSRSDRLAKYNELMRIESSLGENAKYYCGL
EEECCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
AWTS
EECC
>Mature Secondary Structure
MMKKTISSVFAREILDSRGYPTIEVEIELCDGATGRAAVPSGASTGKLEALELRDQDEKR
CCHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCHHHH
YCGKGVLKAVQSVSGVIANKIIGMDAANQSAIDKVLIELDGTKNKSKLGANATLGVSLAV
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHEEEEEECCCCCCHHCCCCCEEHHHHHH
AKAAANSFKTPLYKYLGVGEKQMPVPLINVINGGVHADNKLDFQEFMILPVGAETFSEAI
HHHHHHHHCCHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCHHHEEEEEECHHHHHHHH
RISAEVFHNLRSILKKKGYSTNVGDEGGFAPNIGSTEEALNLIIQAVESAGYSMKNHFAL
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEE
GLDVAASTFYEDGVYKFENRELTSEELVQYYFDLVGKYPIISIEDAMNEDDYEGWKLLTV
EHHHHHHHHHHCCCEEECCCCCCHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCEEEEEE
KLGNKVQLVGDDLFVTNCKLISKGIEEKMANAVLIKPNQIGTLTETFAAIEMAKLNGYRA
EECCEEEEEECCEEEEHHHHHHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHCCEEE
IISHRSGETEDTTISHIAVASNCGQIKTGSLSRSDRLAKYNELMRIESSLGENAKYYCGL
EEECCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEE
AWTS
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA