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The map label for this gene is bioB [H]

Identifier: 189499192

GI number: 189499192

Start: 203498

End: 204499

Strand: Direct

Name: bioB [H]

Synonym: Cphamn1_0208

Alternate gene names: 189499192

Gene position: 203498-204499 (Clockwise)

Preceding gene: 189499191

Following gene: 189499193

Centisome position: 7.44

GC content: 57.68

Gene sequence:

>1002_bases
ATGCGTGTAGAGGAACTACTGAAAAAGAGGCGTTCAGGCGGTGTGCTCTCCCGGGAAGAGCTTGTCTTTCTGCTCGGTTT
GCCGCTGGATTCGGCCGAAACATACATGATCATGGCCGAAGCGAATCGTCTTTCAAAAGAGCATTCAGACGGCAAGGCGG
AAGTTCACGCGCAGTTCGCTCTCAACCTCGCTCCGTGTTCCTGTGACTGTCTGTTCTGTTCCTTCGCGAAGATCAACGAT
GTCTTTCACGAAGCGACGGAGCTGTCCGTGGAACAGGCCGTCTCCTATGCCCGCCAGTTCGAGGTAGATGGAGCGAATGC
GGTGTTCATGATGTCCACAGCCGGCTATCCTTTTGAGCGATTCATCGAGGTGGCCGGAGAAATCAGAAGGAACCTGAACC
CGGAAACGACATTGGTCGCCAATGTCGGCGACCAGACGCTGAAGAACGCGAAGAGGCTCAAGGACGCGGGCTTTGCAGGC
GTGTACCATGCTCTCCGGCTTCGGGAGGGAACGGACAGCACTCTTTCGCCGGAGAAGAGGAAAGAGAGTATCCGGAACTT
TCAGGAAGCCGGGCTCGAAGTCGGAACATGCGTCGAACCTGTAGGTCCGGAGCACACGAACGAGGAACTGGCTGAGATGA
TCGCATTCACGGCGTCCTTTGACCCTTCATTCAGCGGTGCCGCCCGACGTATCCCCATCCCCGGAACGGCGATGGCAAAA
CGCGGAGTGATCAGCGAGTTGCGCATGGCCCAGATCGTTGCCGTCACCCGTCTCGGGATGCCCGATTCCGTCATGGGCAA
CTGCACGCACGAGCCCTGTACGCTCGGGGCGATCGCTGGAGCGAATCTCTTCTGGGCGGAAGTCGGCGCCAATCCCCGGG
ATATCGAGGAAAAGACAGAGGAAGGGCGGGGTGGAACAGTGAGCGACTGCCGGTCTATATTTCGGGAAAGCAACTGGGAT
GTTCTGGACGGCCCGTCGCGCTTTTACAACAGGAAGAGATAG

Upstream 100 bases:

>100_bases
AGGAATGCGCTATATTTTACGGAGTTGTTCAGAAAATCTGTCAGACAGACAACCAGTGATGCTCGATGGCCATCACGTAC
TACAAAAGGATGTATAGCAT

Downstream 100 bases:

>100_bases
AGAAGCGGGGCGCTTGCATCCGGCGCAAAAAAGCATGATGCCGTGAAAATTCCTTGAACCGGGCAGATGCACAAGGACGG
TAACCGTCGATTTCAACAAA

Product: Radical SAM domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 333; Mature: 333

Protein sequence:

>333_residues
MRVEELLKKRRSGGVLSREELVFLLGLPLDSAETYMIMAEANRLSKEHSDGKAEVHAQFALNLAPCSCDCLFCSFAKIND
VFHEATELSVEQAVSYARQFEVDGANAVFMMSTAGYPFERFIEVAGEIRRNLNPETTLVANVGDQTLKNAKRLKDAGFAG
VYHALRLREGTDSTLSPEKRKESIRNFQEAGLEVGTCVEPVGPEHTNEELAEMIAFTASFDPSFSGAARRIPIPGTAMAK
RGVISELRMAQIVAVTRLGMPDSVMGNCTHEPCTLGAIAGANLFWAEVGANPRDIEEKTEEGRGGTVSDCRSIFRESNWD
VLDGPSRFYNRKR

Sequences:

>Translated_333_residues
MRVEELLKKRRSGGVLSREELVFLLGLPLDSAETYMIMAEANRLSKEHSDGKAEVHAQFALNLAPCSCDCLFCSFAKIND
VFHEATELSVEQAVSYARQFEVDGANAVFMMSTAGYPFERFIEVAGEIRRNLNPETTLVANVGDQTLKNAKRLKDAGFAG
VYHALRLREGTDSTLSPEKRKESIRNFQEAGLEVGTCVEPVGPEHTNEELAEMIAFTASFDPSFSGAARRIPIPGTAMAK
RGVISELRMAQIVAVTRLGMPDSVMGNCTHEPCTLGAIAGANLFWAEVGANPRDIEEKTEEGRGGTVSDCRSIFRESNWD
VLDGPSRFYNRKR
>Mature_333_residues
MRVEELLKKRRSGGVLSREELVFLLGLPLDSAETYMIMAEANRLSKEHSDGKAEVHAQFALNLAPCSCDCLFCSFAKIND
VFHEATELSVEQAVSYARQFEVDGANAVFMMSTAGYPFERFIEVAGEIRRNLNPETTLVANVGDQTLKNAKRLKDAGFAG
VYHALRLREGTDSTLSPEKRKESIRNFQEAGLEVGTCVEPVGPEHTNEELAEMIAFTASFDPSFSGAARRIPIPGTAMAK
RGVISELRMAQIVAVTRLGMPDSVMGNCTHEPCTLGAIAGANLFWAEVGANPRDIEEKTEEGRGGTVSDCRSIFRESNWD
VLDGPSRFYNRKR

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 36649; Mature: 36649

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVEELLKKRRSGGVLSREELVFLLGLPLDSAETYMIMAEANRLSKEHSDGKAEVHAQFA
CCHHHHHHHHHCCCCCCHHCEEEEEECCCCCCCEEEEEECHHHHHHHCCCCCCEEEEEEE
LNLAPCSCDCLFCSFAKINDVFHEATELSVEQAVSYARQFEVDGANAVFMMSTAGYPFER
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHH
FIEVAGEIRRNLNPETTLVANVGDQTLKNAKRLKDAGFAGVYHALRLREGTDSTLSPEKR
HHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHH
KESIRNFQEAGLEVGTCVEPVGPEHTNEELAEMIAFTASFDPSFSGAARRIPIPGTAMAK
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
RGVISELRMAQIVAVTRLGMPDSVMGNCTHEPCTLGAIAGANLFWAEVGANPRDIEEKTE
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHCCCEEEEECCCCHHHHHHHHH
EGRGGTVSDCRSIFRESNWDVLDGPSRFYNRKR
CCCCCCHHHHHHHHHCCCCCEECCCHHHHCCCC
>Mature Secondary Structure
MRVEELLKKRRSGGVLSREELVFLLGLPLDSAETYMIMAEANRLSKEHSDGKAEVHAQFA
CCHHHHHHHHHCCCCCCHHCEEEEEECCCCCCCEEEEEECHHHHHHHCCCCCCEEEEEEE
LNLAPCSCDCLFCSFAKINDVFHEATELSVEQAVSYARQFEVDGANAVFMMSTAGYPFER
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHH
FIEVAGEIRRNLNPETTLVANVGDQTLKNAKRLKDAGFAGVYHALRLREGTDSTLSPEKR
HHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCHHHH
KESIRNFQEAGLEVGTCVEPVGPEHTNEELAEMIAFTASFDPSFSGAARRIPIPGTAMAK
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHH
RGVISELRMAQIVAVTRLGMPDSVMGNCTHEPCTLGAIAGANLFWAEVGANPRDIEEKTE
HHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCHHHHCCCEEEEECCCCHHHHHHHHH
EGRGGTVSDCRSIFRESNWDVLDGPSRFYNRKR
CCCCCCHHHHHHHHHCCCCCEECCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]