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The map label for this gene is hemL

Identifier: 189499186

GI number: 189499186

Start: 197802

End: 199097

Strand: Reverse

Name: hemL

Synonym: Cphamn1_0202

Alternate gene names: 189499186

Gene position: 199097-197802 (Counterclockwise)

Preceding gene: 189499187

Following gene: 189499174

Centisome position: 7.28

GC content: 53.55

Gene sequence:

>1296_bases
ATGCCTCAACTCACCAGATCAGCAGAACTCTTTGAAAAAGCGAAACAGTTTATTCCAGGCGGAGTAAACTCGCCGGTTCG
CGCTTTCAAATCCGTCGGCGGCACGCCGATATTCATGGCAAAAGGTCAAGGCGCCTACATGACCGACGTCGACGGCAACA
CATACCTCGACTACGTCGGCTCATGGGGACCGTTCATTCTTGGCAGTATGCATCCTCGCATTACCGCAGCTATCGAGAAC
ACCCTGACAAAAATCGGGACCAGTTTCGGGACACCGATCGAAATGGAAATTGAGATCGCAGAACTTCTGACACAGATCGT
ACCTTCGATCGAAATGGTCCGCATGGTCAACAGCGGTACCGAGGCTACCATGTCAGCTGTTCGCCTTGCAAGAGGCTACA
CGGGCAGGGACAAAATCATCAAGTTTGAAGGATGTTACCATGGTCATGGCGACAGCTTCCTGATCAAGGCTGGCTCAGGC
GCGCTCACTCTCGGCGCTCCGGACAGTCCCGGTGTCACAAAAGGCACCGCAAATGATACGCTGAACGCCAAATACAACGA
TATCGAATCGGTCAGGGTGCTGGTAAAGGAAAACAAGGACAGCATTGCGGCCATCATTATCGAACCTGTAGCGGGCAACA
CCGGCGTGATCCCTGCAAAGACGGACTTCCTCCAGGCGCTCCGCGACCTGTGTACCGAAGAAGGCATCGTCCTGATTTTT
GACGAGGTCATGTGCGGTTTCCGCGTAGCCCTCGGCGGCGCTCAGGAACGTTACGGCATCACTCCCGACCTGACCACCAT
GGGCAAGATCATCGGAGGCGGTCTCCCTGTAGGCGCTTTCGGAGGAAAACGTGAGATCATGCAGCGAGTTGCGCCCATAG
GCGATGTCTATCAGGCGGGAACACTTTCCGGAAATCCTCTGGCGCTTACCGCAGGTCTTGAAACCCTGAAAATCCTGCGC
GACGATAATCCGTACCCGGAACTTGAAAGAAAAGCAGCTTTCCTTGAAGAAGGCTTCAGAGACAACATGAACAAACTCGG
CCTTAGCTATGTCCAGAACCGGGTCGGCTCTATGGCATGTCTCTTCTTTACAGAAACTCCTGTCGAAAACTACGACACGG
CGATCACGTGCGATCTGAAGAAATACGGCAAGTACTACCACTCCATGCTCGATCAGGGTATCTACCTTGCTCCGTCGCAG
TTCGAGGCGATGTTCACCAGTGCGGTCATGACTGACGAAGACCTTGAAAAAACAGTCAAGGCCAACTACGTCGCTCTGCA
GGCTGCTGAAGCGTAA

Upstream 100 bases:

>100_bases
TACTCGCCATATCTGTTTTATATCTTCATCAGTATTTCTATCTTGAGTGTTTGTTAATCACACATCAGGAATTCCGTAAT
CTTTAATCGAATTGGTAGAT

Downstream 100 bases:

>100_bases
GTACTGCTTCCGGAAATCAGAAAGCGTGAGCTGTGTTAAGACGGCTCACGCTTTTTTGTTTCTCTGCTTTTTTCATTTGC
AGCAAACTGGTATCGACTGC

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MPQLTRSAELFEKAKQFIPGGVNSPVRAFKSVGGTPIFMAKGQGAYMTDVDGNTYLDYVGSWGPFILGSMHPRITAAIEN
TLTKIGTSFGTPIEMEIEIAELLTQIVPSIEMVRMVNSGTEATMSAVRLARGYTGRDKIIKFEGCYHGHGDSFLIKAGSG
ALTLGAPDSPGVTKGTANDTLNAKYNDIESVRVLVKENKDSIAAIIIEPVAGNTGVIPAKTDFLQALRDLCTEEGIVLIF
DEVMCGFRVALGGAQERYGITPDLTTMGKIIGGGLPVGAFGGKREIMQRVAPIGDVYQAGTLSGNPLALTAGLETLKILR
DDNPYPELERKAAFLEEGFRDNMNKLGLSYVQNRVGSMACLFFTETPVENYDTAITCDLKKYGKYYHSMLDQGIYLAPSQ
FEAMFTSAVMTDEDLEKTVKANYVALQAAEA

Sequences:

>Translated_431_residues
MPQLTRSAELFEKAKQFIPGGVNSPVRAFKSVGGTPIFMAKGQGAYMTDVDGNTYLDYVGSWGPFILGSMHPRITAAIEN
TLTKIGTSFGTPIEMEIEIAELLTQIVPSIEMVRMVNSGTEATMSAVRLARGYTGRDKIIKFEGCYHGHGDSFLIKAGSG
ALTLGAPDSPGVTKGTANDTLNAKYNDIESVRVLVKENKDSIAAIIIEPVAGNTGVIPAKTDFLQALRDLCTEEGIVLIF
DEVMCGFRVALGGAQERYGITPDLTTMGKIIGGGLPVGAFGGKREIMQRVAPIGDVYQAGTLSGNPLALTAGLETLKILR
DDNPYPELERKAAFLEEGFRDNMNKLGLSYVQNRVGSMACLFFTETPVENYDTAITCDLKKYGKYYHSMLDQGIYLAPSQ
FEAMFTSAVMTDEDLEKTVKANYVALQAAEA
>Mature_430_residues
PQLTRSAELFEKAKQFIPGGVNSPVRAFKSVGGTPIFMAKGQGAYMTDVDGNTYLDYVGSWGPFILGSMHPRITAAIENT
LTKIGTSFGTPIEMEIEIAELLTQIVPSIEMVRMVNSGTEATMSAVRLARGYTGRDKIIKFEGCYHGHGDSFLIKAGSGA
LTLGAPDSPGVTKGTANDTLNAKYNDIESVRVLVKENKDSIAAIIIEPVAGNTGVIPAKTDFLQALRDLCTEEGIVLIFD
EVMCGFRVALGGAQERYGITPDLTTMGKIIGGGLPVGAFGGKREIMQRVAPIGDVYQAGTLSGNPLALTAGLETLKILRD
DNPYPELERKAAFLEEGFRDNMNKLGLSYVQNRVGSMACLFFTETPVENYDTAITCDLKKYGKYYHSMLDQGIYLAPSQF
EAMFTSAVMTDEDLEKTVKANYVALQAAEA

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI13994255, Length=324, Percent_Identity=28.7037037037037, Blast_Score=112, Evalue=6e-25,
Organism=Homo sapiens, GI4557809, Length=303, Percent_Identity=29.3729372937294, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI37574042, Length=331, Percent_Identity=28.0966767371601, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI226442705, Length=331, Percent_Identity=27.7945619335347, Blast_Score=94, Evalue=2e-19,
Organism=Homo sapiens, GI24119277, Length=305, Percent_Identity=26.2295081967213, Blast_Score=93, Evalue=4e-19,
Organism=Homo sapiens, GI226442709, Length=300, Percent_Identity=27.6666666666667, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI284507298, Length=220, Percent_Identity=30.4545454545455, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1786349, Length=419, Percent_Identity=54.653937947494, Blast_Score=477, Evalue=1e-136,
Organism=Escherichia coli, GI1789016, Length=351, Percent_Identity=34.4729344729345, Blast_Score=169, Evalue=3e-43,
Organism=Escherichia coli, GI1788044, Length=323, Percent_Identity=32.1981424148607, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI1789759, Length=320, Percent_Identity=30.9375, Blast_Score=135, Evalue=6e-33,
Organism=Escherichia coli, GI1787560, Length=283, Percent_Identity=32.5088339222615, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI145693181, Length=305, Percent_Identity=29.8360655737705, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1786991, Length=335, Percent_Identity=27.4626865671642, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71992977, Length=338, Percent_Identity=29.2899408284024, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI25144271, Length=334, Percent_Identity=26.9461077844311, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI32564660, Length=322, Percent_Identity=27.639751552795, Blast_Score=91, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI25144274, Length=140, Percent_Identity=30.7142857142857, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323470, Length=330, Percent_Identity=28.7878787878788, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324432, Length=326, Percent_Identity=25.1533742331288, Blast_Score=98, Evalue=2e-21,
Organism=Drosophila melanogaster, GI21356575, Length=334, Percent_Identity=27.8443113772455, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI21357415, Length=299, Percent_Identity=26.4214046822742, Blast_Score=101, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_CHLPB (B3EKJ7)

Other databases:

- EMBL:   CP001101
- RefSeq:   YP_001958656.1
- ProteinModelPortal:   B3EKJ7
- SMR:   B3EKJ7
- GeneID:   6373857
- GenomeReviews:   CP001101_GR
- KEGG:   cpb:Cphamn1_0202
- HOGENOM:   HBG725944
- OMA:   NANIPFF
- ProtClustDB:   PRK00062
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 46504; Mature: 46373

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQLTRSAELFEKAKQFIPGGVNSPVRAFKSVGGTPIFMAKGQGAYMTDVDGNTYLDYVG
CCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCEEEHCCC
SWGPFILGSMHPRITAAIENTLTKIGTSFGTPIEMEIEIAELLTQIVPSIEMVRMVNSGT
CCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCHHHHHHHHCCCH
EATMSAVRLARGYTGRDKIIKFEGCYHGHGDSFLIKAGSGALTLGAPDSPGVTKGTANDT
HHHHHHHHHHHCCCCCCCEEEEECEECCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCC
LNAKYNDIESVRVLVKENKDSIAAIIIEPVAGNTGVIPAKTDFLQALRDLCTEEGIVLIF
CCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
DEVMCGFRVALGGAQERYGITPDLTTMGKIIGGGLPVGAFGGKREIMQRVAPIGDVYQAG
HHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHCC
TLSGNPLALTAGLETLKILRDDNPYPELERKAAFLEEGFRDNMNKLGLSYVQNRVGSMAC
CCCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
LFFTETPVENYDTAITCDLKKYGKYYHSMLDQGIYLAPSQFEAMFTSAVMTDEDLEKTVK
EEEECCCCCCCCCEEEECHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHCCHHHHHHHHH
ANYVALQAAEA
HCEEEEEECCC
>Mature Secondary Structure 
PQLTRSAELFEKAKQFIPGGVNSPVRAFKSVGGTPIFMAKGQGAYMTDVDGNTYLDYVG
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEECCCCCEEEECCCCEEEHCCC
SWGPFILGSMHPRITAAIENTLTKIGTSFGTPIEMEIEIAELLTQIVPSIEMVRMVNSGT
CCCCHHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHCHHHHHHHHCCCH
EATMSAVRLARGYTGRDKIIKFEGCYHGHGDSFLIKAGSGALTLGAPDSPGVTKGTANDT
HHHHHHHHHHHCCCCCCCEEEEECEECCCCCEEEEEECCCEEEECCCCCCCCCCCCCCCC
LNAKYNDIESVRVLVKENKDSIAAIIIEPVAGNTGVIPAKTDFLQALRDLCTEEGIVLIF
CCCCCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEE
DEVMCGFRVALGGAQERYGITPDLTTMGKIIGGGLPVGAFGGKREIMQRVAPIGDVYQAG
HHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCHHHHCC
TLSGNPLALTAGLETLKILRDDNPYPELERKAAFLEEGFRDNMNKLGLSYVQNRVGSMAC
CCCCCCEEEECCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
LFFTETPVENYDTAITCDLKKYGKYYHSMLDQGIYLAPSQFEAMFTSAVMTDEDLEKTVK
EEEECCCCCCCCCEEEECHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHCCHHHHHHHHH
ANYVALQAAEA
HCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA