Click here to switch to the map view.

The map label for this gene is leuA [H]

Identifier: 189499177

GI number: 189499177

Start: 190041

End: 191720

Strand: Direct

Name: leuA [H]

Synonym: Cphamn1_0193

Alternate gene names: 189499177

Gene position: 190041-191720 (Clockwise)

Preceding gene: 189499176

Following gene: 189499178

Centisome position: 6.94

GC content: 50.12

Gene sequence:

>1680_bases
ATGGCGACTATGAACTACGCGAAGTATGAGGCATACCCTGCTGTGGATATAGAGGGCAGAACATGGCCCGATAAGAGAAT
TACAAAAGCACCTCACTGGTGCAGTATTGATTTGAGGGATGGCAACCAGGCTCTTCCGGTTCCTATGAGTGTTGAGGAAA
AAGTCGAGATGTTCAGGCTTCTTGTCGATATCGGATTCAAAGAGATCGAAGTCGGCTTTCCTTCGGCGGCCGCAGTTGAG
TTTCAGTTTGTCAGGCGGTTGATCGAAGAGAATCTTATACCTGAAGATGTGACGATACAGGTGCTGACTCAGGCAAGAGA
GCATCTGATCCGCAAAACATTCGATGCGCTTGAGGGTGTCGGCAATGCTATCGTACATCTCTATAATTCAACATCGACCG
TGCAGCGGGATGTCGTTTTCAGGATGGGGCGCGACGAGATAAAGCGCATTGCCATGGATGGAACGCGGCTTGTTCGTGAA
TTGAAAGAGGCGTCTGGTAATGAGGGGATTCGATTTCAGTACAGCCCTGAAAGTTTTACCGGTACAGAGGTCGATTACGC
GCTCGAGGTCACTCATGCGGTTATGGATGAGTGGGGAGCATCGGAAAACGATCCGATAATTATCAACCTGCCGTCGACTG
TTGAGATGTCGACGCCTAATATATTTGCGGATCGCATTGAGTGGTTCTGCAGAAACATCAGGGAGCGTAAAGCGGTGATT
ATCAGTGTGCATACACATAATGATCGAGGCACAGCAGTAGCTGCTGCAGAGCTTGCGGTCATGGCTGGTGCTGATCGGGT
GGAAGGTGCGCTTTTCGGCAACGGCGAGCGATGCGGTAACCTTGATATTGTCACCATGGCGCTCAATCTGTGTACTCAGG
GAGTGGATCCCGAGCTGGATTTCACGAATCTTGCCCATATCAGCGACGTGTACCGGAAGAATACACGAATGAGCATTCAT
CCCCGACAGCCCTATTCCGGAGAGCTTGTCTACACGGCTTTTTCCGGTTCCCATCAGGATGCCATCAACAAGGGCATGAA
GGCGATCAGGGCATCGGGAAACAACTGCTGGAATGTTCCCTATCTTCCTATTGATCCTCGGGATGTAGGGTGTTCCTATA
AAGCGATTGTCCGGATCAACAGTCAGTCCGGCAAGGGCGGCGTGGCGTATATCATGGAAAATGATTTCGGATATGAAATT
CCGAAATGGATGCAGCCTCATTTCGGTTCGGCGGTACAGTCAAAGGTTGACACTACCGGACGGGAGCTTCTCCCTGAAGA
GATTCGTGAGTTGTTTCATGAAGAGTATATACGCATGCATGATCCGTATCTTATGAAAAAATGCACGATCAAATGGGAGG
ATCAGGATCCTGAACGTGACGATGAGGTCTCGACAACCCTCTCATGCATTCTTCAGCATAACGATACCGAGCGTTCTTTT
ACTGCGAACGGCAACGGGCCGGTTGACGCGTTTGTGAGGGGAATGATGCAGCAGACCGGTCTTGTTTTCAGTGTTGACGA
GTATGCGGAACATTCTATCGGTCACACAGCCGATGCAAAAGCTCTGGCCTATATACGGATAAAAGAGGAGGGAGGACGAG
AGTCGATAGGCGCGGGAATTGATTCCAACATCAGTCTTGCGTCGATCAAGGCGGTGCTCAGCGCTGTGAACAGACTGTAA

Upstream 100 bases:

>100_bases
GTAAGAGATTTCTTGAACAGAAATACCGGCATATGTAGCCACCTGCCTCACAAGGGTCGGTGGCTTTTTTGTTTTACTGA
CAATAAAGTGGAGAACAATA

Downstream 100 bases:

>100_bases
CCTAAGTTGATCGTTTCAACAAATACTTTAAGCTGTCAACTTTATACTATCTAATGCGTTATGCTGAATTTTCTTACAGC
GAGCATCTCACCCGATGGGT

Product: 2-isopropylmalate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase; Alpha-isopropylmalate synthase [H]

Number of amino acids: Translated: 559; Mature: 558

Protein sequence:

>559_residues
MATMNYAKYEAYPAVDIEGRTWPDKRITKAPHWCSIDLRDGNQALPVPMSVEEKVEMFRLLVDIGFKEIEVGFPSAAAVE
FQFVRRLIEENLIPEDVTIQVLTQAREHLIRKTFDALEGVGNAIVHLYNSTSTVQRDVVFRMGRDEIKRIAMDGTRLVRE
LKEASGNEGIRFQYSPESFTGTEVDYALEVTHAVMDEWGASENDPIIINLPSTVEMSTPNIFADRIEWFCRNIRERKAVI
ISVHTHNDRGTAVAAAELAVMAGADRVEGALFGNGERCGNLDIVTMALNLCTQGVDPELDFTNLAHISDVYRKNTRMSIH
PRQPYSGELVYTAFSGSHQDAINKGMKAIRASGNNCWNVPYLPIDPRDVGCSYKAIVRINSQSGKGGVAYIMENDFGYEI
PKWMQPHFGSAVQSKVDTTGRELLPEEIRELFHEEYIRMHDPYLMKKCTIKWEDQDPERDDEVSTTLSCILQHNDTERSF
TANGNGPVDAFVRGMMQQTGLVFSVDEYAEHSIGHTADAKALAYIRIKEEGGRESIGAGIDSNISLASIKAVLSAVNRL

Sequences:

>Translated_559_residues
MATMNYAKYEAYPAVDIEGRTWPDKRITKAPHWCSIDLRDGNQALPVPMSVEEKVEMFRLLVDIGFKEIEVGFPSAAAVE
FQFVRRLIEENLIPEDVTIQVLTQAREHLIRKTFDALEGVGNAIVHLYNSTSTVQRDVVFRMGRDEIKRIAMDGTRLVRE
LKEASGNEGIRFQYSPESFTGTEVDYALEVTHAVMDEWGASENDPIIINLPSTVEMSTPNIFADRIEWFCRNIRERKAVI
ISVHTHNDRGTAVAAAELAVMAGADRVEGALFGNGERCGNLDIVTMALNLCTQGVDPELDFTNLAHISDVYRKNTRMSIH
PRQPYSGELVYTAFSGSHQDAINKGMKAIRASGNNCWNVPYLPIDPRDVGCSYKAIVRINSQSGKGGVAYIMENDFGYEI
PKWMQPHFGSAVQSKVDTTGRELLPEEIRELFHEEYIRMHDPYLMKKCTIKWEDQDPERDDEVSTTLSCILQHNDTERSF
TANGNGPVDAFVRGMMQQTGLVFSVDEYAEHSIGHTADAKALAYIRIKEEGGRESIGAGIDSNISLASIKAVLSAVNRL
>Mature_558_residues
ATMNYAKYEAYPAVDIEGRTWPDKRITKAPHWCSIDLRDGNQALPVPMSVEEKVEMFRLLVDIGFKEIEVGFPSAAAVEF
QFVRRLIEENLIPEDVTIQVLTQAREHLIRKTFDALEGVGNAIVHLYNSTSTVQRDVVFRMGRDEIKRIAMDGTRLVREL
KEASGNEGIRFQYSPESFTGTEVDYALEVTHAVMDEWGASENDPIIINLPSTVEMSTPNIFADRIEWFCRNIRERKAVII
SVHTHNDRGTAVAAAELAVMAGADRVEGALFGNGERCGNLDIVTMALNLCTQGVDPELDFTNLAHISDVYRKNTRMSIHP
RQPYSGELVYTAFSGSHQDAINKGMKAIRASGNNCWNVPYLPIDPRDVGCSYKAIVRINSQSGKGGVAYIMENDFGYEIP
KWMQPHFGSAVQSKVDTTGRELLPEEIRELFHEEYIRMHDPYLMKKCTIKWEDQDPERDDEVSTTLSCILQHNDTERSFT
ANGNGPVDAFVRGMMQQTGLVFSVDEYAEHSIGHTADAKALAYIRIKEEGGRESIGAGIDSNISLASIKAVLSAVNRL

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=536, Percent_Identity=25.1865671641791, Blast_Score=117, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6324225, Length=564, Percent_Identity=43.7943262411348, Blast_Score=496, Evalue=1e-141,
Organism=Saccharomyces cerevisiae, GI6324682, Length=565, Percent_Identity=44.9557522123894, Blast_Score=496, Evalue=1e-141,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013709
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR005668
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like; PF08502 LeuA_dimer [H]

EC number: =2.3.3.13 [H]

Molecular weight: Translated: 62579; Mature: 62448

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS ; PS00815 AIPM_HOMOCIT_SYNTH_1 ; PS00816 AIPM_HOMOCIT_SYNTH_2 ; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATMNYAKYEAYPAVDIEGRTWPDKRITKAPHWCSIDLRDGNQALPVPMSVEEKVEMFRL
CCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHH
LVDIGFKEIEVGFPSAAAVEFQFVRRLIEENLIPEDVTIQVLTQAREHLIRKTFDALEGV
HHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GNAIVHLYNSTSTVQRDVVFRMGRDEIKRIAMDGTRLVRELKEASGNEGIRFQYSPESFT
CCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
GTEVDYALEVTHAVMDEWGASENDPIIINLPSTVEMSTPNIFADRIEWFCRNIRERKAVI
CCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEECCCCHHHHHHHHHHHHHHCCCEEE
ISVHTHNDRGTAVAAAELAVMAGADRVEGALFGNGERCGNLDIVTMALNLCTQGVDPELD
EEEEECCCCCCCHHHHHHHHHCCCCHHCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCCC
FTNLAHISDVYRKNTRMSIHPRQPYSGELVYTAFSGSHQDAINKGMKAIRASGNNCWNVP
CHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEECCC
YLPIDPRDVGCSYKAIVRINSQSGKGGVAYIMENDFGYEIPKWMQPHFGSAVQSKVDTTG
CCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCHHCCCCCCHHHHHHHHCCH
RELLPEEIRELFHEEYIRMHDPYLMKKCTIKWEDQDPERDDEVSTTLSCILQHNDTERSF
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEE
TANGNGPVDAFVRGMMQQTGLVFSVDEYAEHSIGHTADAKALAYIRIKEEGGRESIGAGI
ECCCCCCHHHHHHHHHHHCCCEEEEHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHCCCC
DSNISLASIKAVLSAVNRL
CCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATMNYAKYEAYPAVDIEGRTWPDKRITKAPHWCSIDLRDGNQALPVPMSVEEKVEMFRL
CCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHHHHH
LVDIGFKEIEVGFPSAAAVEFQFVRRLIEENLIPEDVTIQVLTQAREHLIRKTFDALEGV
HHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
GNAIVHLYNSTSTVQRDVVFRMGRDEIKRIAMDGTRLVRELKEASGNEGIRFQYSPESFT
CCEEEEEECCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCC
GTEVDYALEVTHAVMDEWGASENDPIIINLPSTVEMSTPNIFADRIEWFCRNIRERKAVI
CCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCEEECCCCHHHHHHHHHHHHHHCCCEEE
ISVHTHNDRGTAVAAAELAVMAGADRVEGALFGNGERCGNLDIVTMALNLCTQGVDPELD
EEEEECCCCCCCHHHHHHHHHCCCCHHCCEEECCCCCCCCCHHHHHHHHHHHCCCCCCCC
FTNLAHISDVYRKNTRMSIHPRQPYSGELVYTAFSGSHQDAINKGMKAIRASGNNCWNVP
CHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEECCC
YLPIDPRDVGCSYKAIVRINSQSGKGGVAYIMENDFGYEIPKWMQPHFGSAVQSKVDTTG
CCCCCCCCCCCCEEEEEEEECCCCCCCEEEEEECCCCCCCCHHCCCCCCHHHHHHHHCCH
RELLPEEIRELFHEEYIRMHDPYLMKKCTIKWEDQDPERDDEVSTTLSCILQHNDTERSF
HHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCEE
TANGNGPVDAFVRGMMQQTGLVFSVDEYAEHSIGHTADAKALAYIRIKEEGGRESIGAGI
ECCCCCCHHHHHHHHHHHCCCEEEEHHHHHHCCCCCCCCCEEEEEEEECCCCCHHHCCCC
DSNISLASIKAVLSAVNRL
CCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]