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The map label for this gene is nlpE [H]

Identifier: 189499176

GI number: 189499176

Start: 188593

End: 189657

Strand: Direct

Name: nlpE [H]

Synonym: Cphamn1_0192

Alternate gene names: 189499176

Gene position: 188593-189657 (Clockwise)

Preceding gene: 189499175

Following gene: 189499177

Centisome position: 6.89

GC content: 50.33

Gene sequence:

>1065_bases
ATGCGTTATGCTACCGCTGTTTTGAGTATTGTACTGCTTTTTGCCATGGCAGGGTGTCAAGGCAACTATACAAAGCCGGA
TGTGCCTCCTTCCGCAGGAGTATACGGGAAAAACATTACCTATGCAGGTGCGATTCCCTGCGCAGAGTGCACGTTGCAAA
AGCTTACGGTCACGCTTTTTGACGATGGAACGTTTCGCCTGAAACGTGTTTCAGTCGGAGTGCAGGGCGGTGGTACTAAA
GTCGAGTATGATCTTGGACGATGGAACCGGAAAAGTGGCCGTCTGGTATTGAATAACGGAGAAAAGTGGCCGCTGCAGTT
CCGGTATATTTCAGGAAACGAGATACGGCTGCTGGACCAGCGGGGCAATGAAATTGTTTCAAATCTGGACTACAGTCTGA
GAAAGACCTCATTTGTTGATTTCCTCTCCGGTCCCCTGACCATGAACGGTATGTTTCTCTATATGGCTGACGCCTATACG
TTCAGGGAGTGCCGTACCGGAAAAAACTATCCGCTGGTTTTTGCAACGCCGAACAGCTCTATTGAAAAACAGTATCTTGC
TTTGCGTTCCGGGCCGGGAAAGCCTTTGCTGGCAAAGCTATCGGGCAGATTTTCCATAAGAAGGCCTGAGCCGGGGGCAG
CATCCCGGGAGCATATTATCGTTCAGGATTTCAAGCGTTTTCTGCCTCGAGGTACCTGCAGGAATCCCGGTAGTCCGGCA
GCCGCCCTCTACGGCATCTATTGGAAAGTGACAGCTATTTCCGGAAGTTCCGATCTCTTGAAAGTGAGTGGAAAAGTTCC
CAATCTCGTGCTGTCGACATATGGTAACAGTGTCAAGGGATATACAGGATGTAACAGCCTTATGGGGTCATACAAGCACT
CGGGATCAGGGCTGTCATTCTCAAAGCTCTCCACCACACGCATGGCGTGCCCCGGCAGTTCAGGTCAAATAGAAAAGGCA
TTTCTTGCCGCACTCAGGAAGACGACCGGATGGAAAATCAGCGGGAAAACACTGGAACTGTTTGACAGCAGAAAGAGACT
GCTGATGCGTCTGAAAGCGGGTTGA

Upstream 100 bases:

>100_bases
GCTCTGTAGAGAGCGTATCCGTTTTTATATTTCTTTTTTGCTCGCTATACTGTCCGGATAGTTGAGAAGATATTCCATGT
AATAGATCAGGGAGTCATGT

Downstream 100 bases:

>100_bases
GTCAAGTACATCATAAGAGAGGGCACCACTATTTCTTGTCATGAGCTATTCACGTGCGAGGTGAACGGAGCGCAGAAACC
CCTTTCTATCCGCTCAACGA

Product: hypothetical protein

Products: NA

Alternate protein names: Copper homeostasis protein CutF [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MRYATAVLSIVLLFAMAGCQGNYTKPDVPPSAGVYGKNITYAGAIPCAECTLQKLTVTLFDDGTFRLKRVSVGVQGGGTK
VEYDLGRWNRKSGRLVLNNGEKWPLQFRYISGNEIRLLDQRGNEIVSNLDYSLRKTSFVDFLSGPLTMNGMFLYMADAYT
FRECRTGKNYPLVFATPNSSIEKQYLALRSGPGKPLLAKLSGRFSIRRPEPGAASREHIIVQDFKRFLPRGTCRNPGSPA
AALYGIYWKVTAISGSSDLLKVSGKVPNLVLSTYGNSVKGYTGCNSLMGSYKHSGSGLSFSKLSTTRMACPGSSGQIEKA
FLAALRKTTGWKISGKTLELFDSRKRLLMRLKAG

Sequences:

>Translated_354_residues
MRYATAVLSIVLLFAMAGCQGNYTKPDVPPSAGVYGKNITYAGAIPCAECTLQKLTVTLFDDGTFRLKRVSVGVQGGGTK
VEYDLGRWNRKSGRLVLNNGEKWPLQFRYISGNEIRLLDQRGNEIVSNLDYSLRKTSFVDFLSGPLTMNGMFLYMADAYT
FRECRTGKNYPLVFATPNSSIEKQYLALRSGPGKPLLAKLSGRFSIRRPEPGAASREHIIVQDFKRFLPRGTCRNPGSPA
AALYGIYWKVTAISGSSDLLKVSGKVPNLVLSTYGNSVKGYTGCNSLMGSYKHSGSGLSFSKLSTTRMACPGSSGQIEKA
FLAALRKTTGWKISGKTLELFDSRKRLLMRLKAG
>Mature_354_residues
MRYATAVLSIVLLFAMAGCQGNYTKPDVPPSAGVYGKNITYAGAIPCAECTLQKLTVTLFDDGTFRLKRVSVGVQGGGTK
VEYDLGRWNRKSGRLVLNNGEKWPLQFRYISGNEIRLLDQRGNEIVSNLDYSLRKTSFVDFLSGPLTMNGMFLYMADAYT
FRECRTGKNYPLVFATPNSSIEKQYLALRSGPGKPLLAKLSGRFSIRRPEPGAASREHIIVQDFKRFLPRGTCRNPGSPA
AALYGIYWKVTAISGSSDLLKVSGKVPNLVLSTYGNSVKGYTGCNSLMGSYKHSGSGLSFSKLSTTRMACPGSSGQIEKA
FLAALRKTTGWKISGKTLELFDSRKRLLMRLKAG

Specific function: Involved in copper homeostasis. Could be involved in both copper efflux and the delivery of copper to copper-dependent enzymes. When overproduced induces degP through the activation of the two-component system CpxA/CpxR [H]

COG id: COG3015

COG function: function code MP; Uncharacterized lipoprotein NlpE involved in copper resistance

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786390, Length=237, Percent_Identity=28.6919831223629, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007298 [H]

Pfam domain/function: PF04170 NlpE [H]

EC number: NA

Molecular weight: Translated: 38776; Mature: 38776

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYATAVLSIVLLFAMAGCQGNYTKPDVPPSAGVYGKNITYAGAIPCAECTLQKLTVTLF
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHEEEEEEEEEE
DDGTFRLKRVSVGVQGGGTKVEYDLGRWNRKSGRLVLNNGEKWPLQFRYISGNEIRLLDQ
CCCCEEEEEEEEEECCCCEEEEEECCCCCCCCCEEEEECCCCCCEEEEEECCCEEEEEEC
RGNEIVSNLDYSLRKTSFVDFLSGPLTMNGMFLYMADAYTFRECRTGKNYPLVFATPNSS
CCCHHHHHCCHHHHHHHHHHHHCCCEEECEEEEEEECCHHHHHHCCCCCCCEEEECCCCC
IEKQYLALRSGPGKPLLAKLSGRFSIRRPEPGAASREHIIVQDFKRFLPRGTCRNPGSPA
HHHHHHHHHCCCCCCEEEEECCCEEEECCCCCCCCCCEEEHHHHHHHCCCCCCCCCCCCH
AALYGIYWKVTAISGSSDLLKVSGKVPNLVLSTYGNSVKGYTGCNSLMGSYKHSGSGLSF
HEEEEEEEEEEEECCCCCEEEECCCCCEEEEECCCCCCCCCCCHHHHHHCCCCCCCCCEE
SKLSTTRMACPGSSGQIEKAFLAALRKTTGWKISGKTLELFDSRKRLLMRLKAG
HHCCCEEEECCCCCCCHHHHHHHHHHHCCCEEEECCEEHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRYATAVLSIVLLFAMAGCQGNYTKPDVPPSAGVYGKNITYAGAIPCAECTLQKLTVTLF
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHEEEEEEEEEE
DDGTFRLKRVSVGVQGGGTKVEYDLGRWNRKSGRLVLNNGEKWPLQFRYISGNEIRLLDQ
CCCCEEEEEEEEEECCCCEEEEEECCCCCCCCCEEEEECCCCCCEEEEEECCCEEEEEEC
RGNEIVSNLDYSLRKTSFVDFLSGPLTMNGMFLYMADAYTFRECRTGKNYPLVFATPNSS
CCCHHHHHCCHHHHHHHHHHHHCCCEEECEEEEEEECCHHHHHHCCCCCCCEEEECCCCC
IEKQYLALRSGPGKPLLAKLSGRFSIRRPEPGAASREHIIVQDFKRFLPRGTCRNPGSPA
HHHHHHHHHCCCCCCEEEEECCCEEEECCCCCCCCCCEEEHHHHHHHCCCCCCCCCCCCH
AALYGIYWKVTAISGSSDLLKVSGKVPNLVLSTYGNSVKGYTGCNSLMGSYKHSGSGLSF
HEEEEEEEEEEEECCCCCEEEECCCCCEEEEECCCCCCCCCCCHHHHHHCCCCCCCCCEE
SKLSTTRMACPGSSGQIEKAFLAALRKTTGWKISGKTLELFDSRKRLLMRLKAG
HHCCCEEEECCCCCCCHHHHHHHHHHHCCCEEEECCEEHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7635808; 7635807; 9278503 [H]