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The map label for this gene is gcvA [H]

Identifier: 189352691

GI number: 189352691

Start: 789556

End: 790500

Strand: Reverse

Name: gcvA [H]

Synonym: BMULJ_03923

Alternate gene names: 189352691

Gene position: 790500-789556 (Counterclockwise)

Preceding gene: 189352693

Following gene: 189352689

Centisome position: 31.96

GC content: 68.36

Gene sequence:

>945_bases
ATGCGCAAACTCCCTCCCCTGAGCGCGCTGCGCGCGTTCGAAGCCGCGGCGCGACGCGGCGGCTTCAAGGCCGCGGCCGA
GGAACTCAACGTCACGCCGACCGCGATCAGCCACCAGATCCGCCAGCTCGAGGACTGGCTCGGCGTCAAGCTGTTCGAGC
GCCGCACGCGCAGCGTGCGCTTGACCGACGGCGGCACGGCGCTGTTTCCGGCGCTTCGCGACGGATTCGACAGCTTCGAG
CGCGCGATCGAAACGACGCGAAACCTCGACGGCGGCAAGGTCGCGACGCTGACGTCGACGGTCGCGTTCACGGCCAAATG
TCTCGCCGCGCGTGCGGGCGGGTTCCGCGCGGCCTATCCGGACTGGACGCTTCGGCTCGATGCGACGAATGCGGTCGTCG
ATCTCGACCGGTCCGCCGACGCGTCGATCCGGTACGGTCCCGGCTACTACGCGGGGCTCGTGTCGGAGCTGCTGTTCAAG
GATCACTTCGCGCCGGTGTGCCGCCCGCATCTGGCGATCGCGACGGCGCAGGATCTGCAGCGCGCGACGCTGATTCACTT
CGACTGGGGCGCGGCCGTTCGCGACGATCCGCGCGCGATCACGTGGGCGCATTGGCTCGGGGCGGCCGGAATGTCGGGCA
TCGATCCGAACGCGGGGCTGCGGTTCAACGACGAGATTCATGCGATGCAGGCCACGCTCGCCGGACAGGGTGTCGGCCTG
ATCAGCCTGACGCTCGTGGCCGACGAACTCGCGAACGGTACGCTCGTACAGCCGTTCGATCTCGCGCTCGAGAGTTTTCA
GTACGATCTCGTCTATAGTCCGCGGTCGGCGAGTCGACAGGCCACCGCGATATTGAAGCGCTGGGTGCGCGGGGAATTTG
CAGACGCCGACGCGCCCGAGCGGGTATCGGATCGCGGCGTGCTGTGCGAGGCCGCGGAGGAGTGA

Upstream 100 bases:

>100_bases
GATCTCCCGGTTCGGTTCATCGTGGGGACGGCAGTATCGGTCGAGCGCCCGCGCACAACAAATGGTCAATTCTCACCCGA
CAGATGAGCCGTTCTAATCC

Downstream 100 bases:

>100_bases
TGCGGCGCATCGGCCGTATCGATGTGCCGGTCGTTGGCCGCATGCGACGCGGCCGACCGCCCCGCCGAGCGGAAACAGCG
CGGTGATGAGCACGGCCTGC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MRKLPPLSALRAFEAAARRGGFKAAAEELNVTPTAISHQIRQLEDWLGVKLFERRTRSVRLTDGGTALFPALRDGFDSFE
RAIETTRNLDGGKVATLTSTVAFTAKCLAARAGGFRAAYPDWTLRLDATNAVVDLDRSADASIRYGPGYYAGLVSELLFK
DHFAPVCRPHLAIATAQDLQRATLIHFDWGAAVRDDPRAITWAHWLGAAGMSGIDPNAGLRFNDEIHAMQATLAGQGVGL
ISLTLVADELANGTLVQPFDLALESFQYDLVYSPRSASRQATAILKRWVRGEFADADAPERVSDRGVLCEAAEE

Sequences:

>Translated_314_residues
MRKLPPLSALRAFEAAARRGGFKAAAEELNVTPTAISHQIRQLEDWLGVKLFERRTRSVRLTDGGTALFPALRDGFDSFE
RAIETTRNLDGGKVATLTSTVAFTAKCLAARAGGFRAAYPDWTLRLDATNAVVDLDRSADASIRYGPGYYAGLVSELLFK
DHFAPVCRPHLAIATAQDLQRATLIHFDWGAAVRDDPRAITWAHWLGAAGMSGIDPNAGLRFNDEIHAMQATLAGQGVGL
ISLTLVADELANGTLVQPFDLALESFQYDLVYSPRSASRQATAILKRWVRGEFADADAPERVSDRGVLCEAAEE
>Mature_314_residues
MRKLPPLSALRAFEAAARRGGFKAAAEELNVTPTAISHQIRQLEDWLGVKLFERRTRSVRLTDGGTALFPALRDGFDSFE
RAIETTRNLDGGKVATLTSTVAFTAKCLAARAGGFRAAYPDWTLRLDATNAVVDLDRSADASIRYGPGYYAGLVSELLFK
DHFAPVCRPHLAIATAQDLQRATLIHFDWGAAVRDDPRAITWAHWLGAAGMSGIDPNAGLRFNDEIHAMQATLAGQGVGL
ISLTLVADELANGTLVQPFDLALESFQYDLVYSPRSASRQATAILKRWVRGEFADADAPERVSDRGVLCEAAEE

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=295, Percent_Identity=34.5762711864407, Blast_Score=157, Evalue=6e-40,
Organism=Escherichia coli, GI1786448, Length=297, Percent_Identity=32.996632996633, Blast_Score=131, Evalue=5e-32,
Organism=Escherichia coli, GI1788706, Length=269, Percent_Identity=31.5985130111524, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI87081978, Length=305, Percent_Identity=28.8524590163934, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1787128, Length=271, Percent_Identity=26.5682656826568, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786401, Length=257, Percent_Identity=26.0700389105058, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI145693105, Length=187, Percent_Identity=27.807486631016, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI157672245, Length=174, Percent_Identity=31.0344827586207, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786508, Length=302, Percent_Identity=25.8278145695364, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787949, Length=80, Percent_Identity=47.5, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34145; Mature: 34145

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLPPLSALRAFEAAARRGGFKAAAEELNVTPTAISHQIRQLEDWLGVKLFERRTRSVR
CCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LTDGGTALFPALRDGFDSFERAIETTRNLDGGKVATLTSTVAFTAKCLAARAGGFRAAYP
EECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEECC
DWTLRLDATNAVVDLDRSADASIRYGPGYYAGLVSELLFKDHFAPVCRPHLAIATAQDLQ
CEEEEEECCCEEEECCCCCCCCEEECCCHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHH
RATLIHFDWGAAVRDDPRAITWAHWLGAAGMSGIDPNAGLRFNDEIHAMQATLAGQGVGL
HEEEEEEECCCCCCCCCCEEEHHHHHHCCCCCCCCCCCCCEECCHHHHHHHHHCCCCCCC
ISLTLVADELANGTLVQPFDLALESFQYDLVYSPRSASRQATAILKRWVRGEFADADAPE
EEHHHHHHHHCCCCEECHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCC
RVSDRGVLCEAAEE
HHCCCCCEECCCCC
>Mature Secondary Structure
MRKLPPLSALRAFEAAARRGGFKAAAEELNVTPTAISHQIRQLEDWLGVKLFERRTRSVR
CCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEE
LTDGGTALFPALRDGFDSFERAIETTRNLDGGKVATLTSTVAFTAKCLAARAGGFRAAYP
EECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHCCCCEEECC
DWTLRLDATNAVVDLDRSADASIRYGPGYYAGLVSELLFKDHFAPVCRPHLAIATAQDLQ
CEEEEEECCCEEEECCCCCCCCEEECCCHHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHH
RATLIHFDWGAAVRDDPRAITWAHWLGAAGMSGIDPNAGLRFNDEIHAMQATLAGQGVGL
HEEEEEEECCCCCCCCCCEEEHHHHHHCCCCCCCCCCCCCEECCHHHHHHHHHCCCCCCC
ISLTLVADELANGTLVQPFDLALESFQYDLVYSPRSASRQATAILKRWVRGEFADADAPE
EEHHHHHHHHCCCCEECHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCC
RVSDRGVLCEAAEE
HHCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]