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The map label for this gene is acnB [H]

Identifier: 189352150

GI number: 189352150

Start: 177359

End: 179944

Strand: Direct

Name: acnB [H]

Synonym: BMULJ_03368

Alternate gene names: 189352150

Gene position: 177359-179944 (Clockwise)

Preceding gene: 189352148

Following gene: 189352151

Centisome position: 7.17

GC content: 65.27

Gene sequence:

>2586_bases
ATGCTTGAAAACTTTCGTGCTCACGTGGCCGCCCGCGCCGCGCTCGGTATTCCCCCCCTGCCGCTGACGGCCCAGCAAAC
CGCCGAACTGGTGGAACTGCTGACGAACCCGCCGGCCGGCGAAGAGCAGACGCTGCTCGATCTGATCACCCATCGCGTGC
CGGCAGGCGTCGACGAAGCGGCGCGCGTGAAGGCCGGCTTCCTCGCGGCCGTCGCGAAGGGCGAGACCGCTTGCCCGCTG
ATCTCGCGTGCACGTGCCACCGAACTGCTCGGCACGATGCTCGGCGGCTACAACATTCAGCCGCTGATCGAGCTGCTGTC
CGACGCCGAAGTCGGCGCGGTCGCCGCCGATGCCCTGAAGAAAACGCTGCTGATGTTCGACCAGTTCCACGACGTGAAGG
AACTCGCCGACAAGGGCAACGCGAACGCGAAGGCCGTGATGCAGAGCTGGGCCGACGCCGAATGGTTCACGAGCCGTCCG
GAAGTGCCGGAAAGCCTCACGGTCACCGTGTTCAAGGTGCCGGGCGAAACCAACACCGACGATCTGTCGCCGGCGCCGGA
CGCAACCACGCGCCCGGACATCCCGCTGCACGCGCTCGCGATGCTGAAGAACGCGCGTCCCGGCATCACGCCGGAAGAAG
ACGGCAAGCGCGGCCCGGTCAAATTCATCGAATCGCTGAAGGAAAAGGGCCATCTCGTCGCGTACGTCGGCGACGTGGTC
GGCACGGGTTCGTCGCGCAAGTCGGCCACCAACTCGGTGCTGTGGTTCACCGGCCAGGACATTCCGTACGTGCCGAACAA
GCGCTTCGGCGGCGTGTGCCTCGGCGGCAAGATCGCGCCGATCTTCTACAACACGATGGAAGATGCCGGCGCGCTGCCGA
TCGAGCTCGACGTGTCGAAGATGGAAATGGGCGACGTCATCGAGCTGCGTCCGTACGAAGGCAAGGCGCTGAAGAACGGC
GAAGTGATCGCCGAATTCCAGGTCAAGTCCGACGTGCTGTTCGATGAAGTGCGCGCAGGCGGCCGCATTCCGCTGATCAT
CGGCCGCGGCCTCACGGCGAAGGCGCGCGAAGCGCTCGGTCTGCCGCCGTCGACGCTGTTCCGTCTGCCGCATCAGCCGG
CCGACAGCGGCAAGGGCTTCACGCTCGCGCAGAAGATGGTCGGCCGCGCATGCGGTCTGCCGGAAGGCAAGGGCGTGCGT
CCGGGCACGTACTGCGAACCGAAGATGACCTCGGTCGGCTCGCAGGACACGACGGGCCCGATGACGCGCGACGAGCTGAA
GGATCTCGCGTGCCTCGGCTTCTCGGCCGATCTCGTAATGCAGTCGTTCTGCCACACGGCCGCCTATCCGAAGCCGGTCG
ACGTGAAGACGCACCAGACGCTGCCGAACTTCATCAGCACGCGCGGCGGCATCTCGCTGCGCCCGGGCGACGGCGTGATC
CACTCGTGGCTGAACCGCATGCTGCTGCCCGACACCGTCGGCACCGGCGGCGACTCGCACACGCGCTTCCCGATCGGCAT
CAGCTTCCCGGCAGGTTCGGGCCTCGTCGCGTTCGCGGCGGCCACCGGCACGATGCCGCTCGACATGCCGGAATCGGTGC
TGGTCCGCTTCAAGGGCAAGATGCAGCCGGGCGTGACGTTGCGCGATCTGGTCAACGCGATTCCGCTGTACGCGATCAAG
CAGGGCATGCTGACGGTCGCGAAGCAGGGCAAGAAGAACATCTTCTCGGGCCGCATTCTCGAGATCGAAGGCCTGCCGGA
TCTGAAGGTCGAACAGGCGTTCGAACTGTCGGATGCGTCGGCCGAGCGTTCGGCGGCCGGTTGCACGGTGCGTCTGAACA
AGGAACCGATCATCGAGTACCTGAACAGCAACATCACGCTGCTGAAGTGGATGATCGCGCAGGGCTATCAGGATGCGCGC
AGCCTGCAGCGCCGGATCGCGGCGATGGAGCAGTGGCTCGCCGATCCGAAGCTGCTCGAGCCGGACGCGGACGCCGAATA
CGCAGCCGTGATCGAGATCGACCTCGCGGACATCCACGAGCCGATCGTCGCCTGCCCGAACGATCCGGACGACGTGAAGA
CGCTGTCCGACGTCGCCGGCGCGAAGATCGACGAAGTGTTCATCGGTTCGTGCATGACCAACATCGGCCACTTCCGTGCG
GCATCGAAGCTGCTCGAAGGCAAGCGCGACATTCCGGTCAAGCTGTGGGTCGCACCGCCGACCAAGATGGACCAGAAGCA
GCTGACCGAGGAAGGTCACTACGGCGTGTTCGGCACGGCCGGCGCGCGCACCGAAATGCCGGGCTGCTCGCTCTGCATGG
GCAACCAGGCGCAGGTGCGCGAAGGCGCGACGGTCATGTCGACGTCGACCCGCAACTTCCCGAACCGTCTCGGCAAGAAC
ACGAACGTGTATCTCGGCTCGGCGGAACTGGCGGCGATCTGCTCGCGACTCGGCCGGATCCCGACGAAGGAGGAGTACAT
GGCCGACATCGGCGTGATCAACGCGAACGGCGACAAGATCTACAAGTACATGAACTTCGACCAGATCGAAGACTTCAAGG
AAGTCGCCGACACCGTGCAGGTCTAA

Upstream 100 bases:

>100_bases
CCCGCCATTGCGATTAGAATCGGCGTCGATGCAGTGCCCGGAACAGCCTCGGAGCGCCTGAAAAGCCTTCCCCTGAAACG
CAACATCAGCAGGTCCCCCC

Downstream 100 bases:

>100_bases
GCACGCAGTCGGGCTGCGGCGATGCAGGTTCGCCGCAGCGTCCGCGACAGGGCGCCGCCAGGGAAACCGGGCGGCGCCTT
CTTTTTTGCGAGACGTCGAC

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase 2; 2-methylisocitrate dehydratase; Citrate hydro-lyase 2 [H]

Number of amino acids: Translated: 861; Mature: 861

Protein sequence:

>861_residues
MLENFRAHVAARAALGIPPLPLTAQQTAELVELLTNPPAGEEQTLLDLITHRVPAGVDEAARVKAGFLAAVAKGETACPL
ISRARATELLGTMLGGYNIQPLIELLSDAEVGAVAADALKKTLLMFDQFHDVKELADKGNANAKAVMQSWADAEWFTSRP
EVPESLTVTVFKVPGETNTDDLSPAPDATTRPDIPLHALAMLKNARPGITPEEDGKRGPVKFIESLKEKGHLVAYVGDVV
GTGSSRKSATNSVLWFTGQDIPYVPNKRFGGVCLGGKIAPIFYNTMEDAGALPIELDVSKMEMGDVIELRPYEGKALKNG
EVIAEFQVKSDVLFDEVRAGGRIPLIIGRGLTAKAREALGLPPSTLFRLPHQPADSGKGFTLAQKMVGRACGLPEGKGVR
PGTYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHQTLPNFISTRGGISLRPGDGVI
HSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGTMPLDMPESVLVRFKGKMQPGVTLRDLVNAIPLYAIK
QGMLTVAKQGKKNIFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCTVRLNKEPIIEYLNSNITLLKWMIAQGYQDAR
SLQRRIAAMEQWLADPKLLEPDADAEYAAVIEIDLADIHEPIVACPNDPDDVKTLSDVAGAKIDEVFIGSCMTNIGHFRA
ASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLGKN
TNVYLGSAELAAICSRLGRIPTKEEYMADIGVINANGDKIYKYMNFDQIEDFKEVADTVQV

Sequences:

>Translated_861_residues
MLENFRAHVAARAALGIPPLPLTAQQTAELVELLTNPPAGEEQTLLDLITHRVPAGVDEAARVKAGFLAAVAKGETACPL
ISRARATELLGTMLGGYNIQPLIELLSDAEVGAVAADALKKTLLMFDQFHDVKELADKGNANAKAVMQSWADAEWFTSRP
EVPESLTVTVFKVPGETNTDDLSPAPDATTRPDIPLHALAMLKNARPGITPEEDGKRGPVKFIESLKEKGHLVAYVGDVV
GTGSSRKSATNSVLWFTGQDIPYVPNKRFGGVCLGGKIAPIFYNTMEDAGALPIELDVSKMEMGDVIELRPYEGKALKNG
EVIAEFQVKSDVLFDEVRAGGRIPLIIGRGLTAKAREALGLPPSTLFRLPHQPADSGKGFTLAQKMVGRACGLPEGKGVR
PGTYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHQTLPNFISTRGGISLRPGDGVI
HSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGTMPLDMPESVLVRFKGKMQPGVTLRDLVNAIPLYAIK
QGMLTVAKQGKKNIFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCTVRLNKEPIIEYLNSNITLLKWMIAQGYQDAR
SLQRRIAAMEQWLADPKLLEPDADAEYAAVIEIDLADIHEPIVACPNDPDDVKTLSDVAGAKIDEVFIGSCMTNIGHFRA
ASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLGKN
TNVYLGSAELAAICSRLGRIPTKEEYMADIGVINANGDKIYKYMNFDQIEDFKEVADTVQV
>Mature_861_residues
MLENFRAHVAARAALGIPPLPLTAQQTAELVELLTNPPAGEEQTLLDLITHRVPAGVDEAARVKAGFLAAVAKGETACPL
ISRARATELLGTMLGGYNIQPLIELLSDAEVGAVAADALKKTLLMFDQFHDVKELADKGNANAKAVMQSWADAEWFTSRP
EVPESLTVTVFKVPGETNTDDLSPAPDATTRPDIPLHALAMLKNARPGITPEEDGKRGPVKFIESLKEKGHLVAYVGDVV
GTGSSRKSATNSVLWFTGQDIPYVPNKRFGGVCLGGKIAPIFYNTMEDAGALPIELDVSKMEMGDVIELRPYEGKALKNG
EVIAEFQVKSDVLFDEVRAGGRIPLIIGRGLTAKAREALGLPPSTLFRLPHQPADSGKGFTLAQKMVGRACGLPEGKGVR
PGTYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHQTLPNFISTRGGISLRPGDGVI
HSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGTMPLDMPESVLVRFKGKMQPGVTLRDLVNAIPLYAIK
QGMLTVAKQGKKNIFSGRILEIEGLPDLKVEQAFELSDASAERSAAGCTVRLNKEPIIEYLNSNITLLKWMIAQGYQDAR
SLQRRIAAMEQWLADPKLLEPDADAEYAAVIEIDLADIHEPIVACPNDPDDVKTLSDVAGAKIDEVFIGSCMTNIGHFRA
ASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCSLCMGNQAQVREGATVMSTSTRNFPNRLGKN
TNVYLGSAELAAICSRLGRIPTKEEYMADIGVINANGDKIYKYMNFDQIEDFKEVADTVQV

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=862, Percent_Identity=74.7099767981439, Blast_Score=1287, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=375, Percent_Identity=25.3333333333333, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6321429, Length=360, Percent_Identity=24.4444444444444, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 92760; Mature: 92760

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLENFRAHVAARAALGIPPLPLTAQQTAELVELLTNPPAGEEQTLLDLITHRVPAGVDEA
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHH
ARVKAGFLAAVAKGETACPLISRARATELLGTMLGGYNIQPLIELLSDAEVGAVAADALK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
KTLLMFDQFHDVKELADKGNANAKAVMQSWADAEWFTSRPEVPESLTVTVFKVPGETNTD
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHCCCCCCCCCCEEEEEEECCCCCCCC
DLSPAPDATTRPDIPLHALAMLKNARPGITPEEDGKRGPVKFIESLKEKGHLVAYVGDVV
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECHHH
GTGSSRKSATNSVLWFTGQDIPYVPNKRFGGVCLGGKIAPIFYNTMEDAGALPIELDVSK
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEHEEECHHHCCCCEEEEEHHH
MEMGDVIELRPYEGKALKNGEVIAEFQVKSDVLFDEVRAGGRIPLIIGRGLTAKAREALG
HCCCCEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHCC
LPPSTLFRLPHQPADSGKGFTLAQKMVGRACGLPEGKGVRPGTYCEPKMTSVGSQDTTGP
CCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCCCC
MTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHQTLPNFISTRGGISLRPGDGVI
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEECCCCHHH
HSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGTMPLDMPESVLVRFKGK
HHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCCHHHHHHCCCC
MQPGVTLRDLVNAIPLYAIKQGMLTVAKQGKKNIFSGRILEIEGLPDLKVEQAFELSDAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHCCCCCC
AERSAAGCTVRLNKEPIIEYLNSNITLLKWMIAQGYQDARSLQRRIAAMEQWLADPKLLE
HHHCCCCCEEEECCHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCC
PDADAEYAAVIEIDLADIHEPIVACPNDPDDVKTLSDVAGAKIDEVFIGSCMTNIGHFRA
CCCCCCEEEEEEEEHHHHCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCSLCMGNQAQVR
HHHHHCCCCCCCEEEEECCCCCCCHHHHHCCCCCCEEECCCCCCCCCCCCEECCCHHHHH
EGATVMSTSTRNFPNRLGKNTNVYLGSAELAAICSRLGRIPTKEEYMADIGVINANGDKI
CCCEEEECCCCCHHHHHCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHCEEEECCCCEE
YKYMNFDQIEDFKEVADTVQV
EEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLENFRAHVAARAALGIPPLPLTAQQTAELVELLTNPPAGEEQTLLDLITHRVPAGVDEA
CCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCHH
ARVKAGFLAAVAKGETACPLISRARATELLGTMLGGYNIQPLIELLSDAEVGAVAADALK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHHHHH
KTLLMFDQFHDVKELADKGNANAKAVMQSWADAEWFTSRPEVPESLTVTVFKVPGETNTD
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHCCCCCCCCCCEEEEEEECCCCCCCC
DLSPAPDATTRPDIPLHALAMLKNARPGITPEEDGKRGPVKFIESLKEKGHLVAYVGDVV
CCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEECHHH
GTGSSRKSATNSVLWFTGQDIPYVPNKRFGGVCLGGKIAPIFYNTMEDAGALPIELDVSK
CCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEECCEEEEHEEECHHHCCCCEEEEEHHH
MEMGDVIELRPYEGKALKNGEVIAEFQVKSDVLFDEVRAGGRIPLIIGRGLTAKAREALG
HCCCCEEEECCCCCCCCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHCC
LPPSTLFRLPHQPADSGKGFTLAQKMVGRACGLPEGKGVRPGTYCEPKMTSVGSQDTTGP
CCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHCCCCCCCCCC
MTRDELKDLACLGFSADLVMQSFCHTAAYPKPVDVKTHQTLPNFISTRGGISLRPGDGVI
CCHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEECCCCHHH
HSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGTMPLDMPESVLVRFKGK
HHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCCCCCHHHHHHCCCC
MQPGVTLRDLVNAIPLYAIKQGMLTVAKQGKKNIFSGRILEIEGLPDLKVEQAFELSDAS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHCCCCCC
AERSAAGCTVRLNKEPIIEYLNSNITLLKWMIAQGYQDARSLQRRIAAMEQWLADPKLLE
HHHCCCCCEEEECCHHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCC
PDADAEYAAVIEIDLADIHEPIVACPNDPDDVKTLSDVAGAKIDEVFIGSCMTNIGHFRA
CCCCCCEEEEEEEEHHHHCCCEEECCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
ASKLLEGKRDIPVKLWVAPPTKMDQKQLTEEGHYGVFGTAGARTEMPGCSLCMGNQAQVR
HHHHHCCCCCCCEEEEECCCCCCCHHHHHCCCCCCEEECCCCCCCCCCCCEECCCHHHHH
EGATVMSTSTRNFPNRLGKNTNVYLGSAELAAICSRLGRIPTKEEYMADIGVINANGDKI
CCCEEEECCCCCHHHHHCCCCEEEECCHHHHHHHHHHCCCCCHHHHHHHCEEEECCCCEE
YKYMNFDQIEDFKEVADTVQV
EEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]