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The map label for this gene is galE [H]

Identifier: 189352136

GI number: 189352136

Start: 163120

End: 163953

Strand: Direct

Name: galE [H]

Synonym: BMULJ_03354

Alternate gene names: 189352136

Gene position: 163120-163953 (Clockwise)

Preceding gene: 189352134

Following gene: 189352141

Centisome position: 6.6

GC content: 66.79

Gene sequence:

>834_bases
ATGAACGCCTCAACCACCGGTACGCGCAAACCGTTCCGGCGCCTGCTGCTGACGGGCGCGGCCGGCAACCTCGGCCGTCA
GCTGCGCGGCGCGCTCGCGGAATGGGCCGACATCGTGCGCGTCAGCGACATCGCGTCGCTCGGCGAAGCCGCTGCACATG
AAGAAGCGCAGGTCGTCGATCTGGCCGACCGGCCGGCCGTGATGCACCTCGTCGAGGGCGTCGATGCGATCGTGCATCTC
GGCGGCATCTCGGTGGATGCGCCGTTCGACGATCTGGTCGGCGCGAACATCACCGGCACGTACAACATCTACGAAGCCGC
GCGCCGGCACGGCGTGAAGCGGATCGTGTTCGCGAGCTCGAACCATGCGATCGGCTTCCATCCCGTCACCGAAGTGCTCG
ACGCTGACGCGCCGCAGCGCCCGGACAGCCTGTACGGCGTGACGAAATGCTTCGGCGAGGCGCTGTCGCGCTACTACTTC
GATCGCTTCGGAATCGAAACGGTGTGCCTGCGGATCGGTTCGTCGTTCGAGGAGCCGAAGAATCCGCGCATGCTCGTCAC
GTTCCTCAGCTATCGCGACTTCATCGAGCTCGTGCGCTGCTCGCTGCTGACGAACCGCGTCGGACACGCGATCGTGTACG
GCGTGTCCGACAACCCGGTGAAGTGGTGGGACAACACGAAGGCCGGTTTTCTCGGCTTCCGGCCGCGCGACAGTTCCGAG
CAGTTCGCGGGTCTGTTCCCGATCGCCGCGCCGACGGCCGATTACGACGATCCGGCGCAGCGGTTTCAGGGCGGCGCGTT
CGTCGTCGGCGAGCCGATGGAGCGGCGCGCGTGA

Upstream 100 bases:

>100_bases
CGTATCGGCCATTGACGCGTCGAATTTGTCCCGCGTATAGTTGGAAAACGTTTTCCACCTCAATACGATTCAACCGCAAC
AACCGATTCAAGGAGATTCG

Downstream 100 bases:

>100_bases
AGCTGCGCCGATGAAGCGGCCGCGCGGCGCGCGATCGTTGCGCCGCCGCGGTGATCGGGGCAGCGCGTCAGCGCAGGATG
TCGGTCGCCTTGTGATGTTC

Product: UDP-glucose 4-epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MNASTTGTRKPFRRLLLTGAAGNLGRQLRGALAEWADIVRVSDIASLGEAAAHEEAQVVDLADRPAVMHLVEGVDAIVHL
GGISVDAPFDDLVGANITGTYNIYEAARRHGVKRIVFASSNHAIGFHPVTEVLDADAPQRPDSLYGVTKCFGEALSRYYF
DRFGIETVCLRIGSSFEEPKNPRMLVTFLSYRDFIELVRCSLLTNRVGHAIVYGVSDNPVKWWDNTKAGFLGFRPRDSSE
QFAGLFPIAAPTADYDDPAQRFQGGAFVVGEPMERRA

Sequences:

>Translated_277_residues
MNASTTGTRKPFRRLLLTGAAGNLGRQLRGALAEWADIVRVSDIASLGEAAAHEEAQVVDLADRPAVMHLVEGVDAIVHL
GGISVDAPFDDLVGANITGTYNIYEAARRHGVKRIVFASSNHAIGFHPVTEVLDADAPQRPDSLYGVTKCFGEALSRYYF
DRFGIETVCLRIGSSFEEPKNPRMLVTFLSYRDFIELVRCSLLTNRVGHAIVYGVSDNPVKWWDNTKAGFLGFRPRDSSE
QFAGLFPIAAPTADYDDPAQRFQGGAFVVGEPMERRA
>Mature_277_residues
MNASTTGTRKPFRRLLLTGAAGNLGRQLRGALAEWADIVRVSDIASLGEAAAHEEAQVVDLADRPAVMHLVEGVDAIVHL
GGISVDAPFDDLVGANITGTYNIYEAARRHGVKRIVFASSNHAIGFHPVTEVLDADAPQRPDSLYGVTKCFGEALSRYYF
DRFGIETVCLRIGSSFEEPKNPRMLVTFLSYRDFIELVRCSLLTNRVGHAIVYGVSDNPVKWWDNTKAGFLGFRPRDSSE
QFAGLFPIAAPTADYDDPAQRFQGGAFVVGEPMERRA

Specific function: INVOLVED IN THE SYNTHESIS OF ENTEROBACTERIAL COMMON ANTIGEN (ECA) AND REQUIRED FOR SYNTHESIS OF LIPOPOLYSACCHARIDE O-SIDE CHAINS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 30333; Mature: 30333

Theoretical pI: Translated: 6.17; Mature: 6.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNASTTGTRKPFRRLLLTGAAGNLGRQLRGALAEWADIVRVSDIASLGEAAAHEEAQVVD
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LADRPAVMHLVEGVDAIVHLGGISVDAPFDDLVGANITGTYNIYEAARRHGVKRIVFASS
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECC
NHAIGFHPVTEVLDADAPQRPDSLYGVTKCFGEALSRYYFDRFGIETVCLRIGSSFEEPK
CCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NPRMLVTFLSYRDFIELVRCSLLTNRVGHAIVYGVSDNPVKWWDNTKAGFLGFRPRDSSE
CCEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEECCCCCCCEEECCCCCCCC
QFAGLFPIAAPTADYDDPAQRFQGGAFVVGEPMERRA
HHHCCCCCCCCCCCCCCHHHHHCCCEEEECCCHHCCC
>Mature Secondary Structure
MNASTTGTRKPFRRLLLTGAAGNLGRQLRGALAEWADIVRVSDIASLGEAAAHEEAQVVD
CCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEE
LADRPAVMHLVEGVDAIVHLGGISVDAPFDDLVGANITGTYNIYEAARRHGVKRIVFASS
CCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECC
NHAIGFHPVTEVLDADAPQRPDSLYGVTKCFGEALSRYYFDRFGIETVCLRIGSSFEEPK
CCCCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
NPRMLVTFLSYRDFIELVRCSLLTNRVGHAIVYGVSDNPVKWWDNTKAGFLGFRPRDSSE
CCEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCEEECCCCCCCEEECCCCCCCC
QFAGLFPIAAPTADYDDPAQRFQGGAFVVGEPMERRA
HHHCCCCCCCCCCCCCCHHHHHCCCEEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.093 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: p-Chloromercuribenzoate; TMP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8763940; 9387221; 9384377 [H]