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The map label for this gene is ligA

Identifier: 189184847

GI number: 189184847

Start: 1974465

End: 1976564

Strand: Reverse

Name: ligA

Synonym: OTT_1940

Alternate gene names: 189184847

Gene position: 1976564-1974465 (Counterclockwise)

Preceding gene: 189184848

Following gene: 189184846

Centisome position: 98.39

GC content: 30.29

Gene sequence:

>2100_bases
ATGAATAAGCTAGATATTAGCACGATTAATATTGAACAATTAACATCAGATCATGCTAAGAAAATTGTGGATTTTTTAGC
TTTGGAATTACAAAAATATGATCAAGCTTATTATAGTGACAATAATCCTTTAGTTACTGATGCACAATACGATGTTCTGA
AAAATTTAAATAATAAAATTGTAGCCAAGTTTCCACATTTAGCATTAGTAAATGATCATTCTAAAAAAGTTGGATTTACA
CCAAGTGCGCAGTTTTCTAAGGTGGTACATCTTAAGCCAATGCTATCATTAGCAAATGGTTTTTGTGTAGAGGATATTAG
CAATTTTATTACTAAGATACAAAATTTTTTAAAAATAGATCATTGCCCCAAAATTGTTTGTGAATATAAAATTGATGGAT
TGTCATTTAATGCTAGATATGAGTATGGGGTCTTAACCTTAGCAAGCACTAGAGGTGATGGGCAGATTGGAGAAAATATT
ACTGAAAACTTAAAAACAATACAATCTTTCCCTCAAACCTTGCCTATTACTGATAAAGTATTTGAAGTCAGAGGTGAAAT
TTATATTACTAATAATGATTTTAGAGTTTTAAATATTCAACAACAGAAACTAGGTAAAGCTTTATTTTCTAATCCACGTA
ATGCTGCTGCTGGTTCAATTAGACAACTTGATCCTGCTATTACAGCTCAAAGACCACTAAAATATTTTGTTTATGCATTA
GGAGAAGTAACTAATCACCATTTTGCTAGTACGCAGTTTGAATTACTACAGAAACTATCTCAGCTTAAGTTTTCCGTTAA
TACAGATTATATATTATCAGATAATCTGCATTCAATGATAGAATTTTATAATAACGTTGCTACAGAACGCAACAATTTAG
CATTTGAGATAGATGGAGTAGTGTACAAGGTGAATGATTTTGCTTTGCAGGAAAGACTTGGAGCTACTAGTACTAGTCCA
CGTTTTGCGATTGCATATAAATTTCCAGCATTAATTGGTAGAACGAAGATAACTAACATTACTTTACAGGTTGGCAAAAC
AGGAGCTGTTACTCCTGTAGCATGGCTTATCCCTATTAATATTGCCGGAGTAACAATATCAAGAGCAACGTTGCATAATA
AACAAGAAATAGAATCAAAAGATATTAGAATTGGCGATTATGTGTTTTTACATCGAGCAGGAGACGTTATTCCAAAAATT
AATGGTGTTGATTTATCAGCTAGAGATACACAATCCTCTGAAAGGTTTATCTTTCCTACAACATGTCCTTCTTGTAATCA
GAATTTAGTTGTTAATGAAGGAGAAACAGTAGCAAGGTGTGGTAACAGTTTAGCATGCCAAGCCCAAATTTATGAACGTA
TCTGTCACTTTGTATCAAAAGATGCATTAAATATTGATGGATTAGGTAAACAGAGAATACAGTTTTTATTAGATAATAAG
TACATAGTAAATATAGTTGATATATTTTTGTTAGAAGAGAATAATAAATTTTTATATTCAAATAAACTTGAAGATATAGC
TGGCTGGGGAATAAAATCTGTAAACAAGTTATTTCAAAATATTAATCAAGCTAAAAATGTTATTCTAGACAAATTTATTT
ATGCGTTAGCCATTAAACATGTTGGTAAATATAGTGCTAAATTATTAGCTAAAGAATTTAAAACAGCAAAAAATTTTATA
AACCAATCACTAAAACTAGCAAATAACGCTACAGAAATATATGAAAAATTACGTAATATAGAGGGTGTAGGAGTAAAAAC
TGCTGACCAGTTAAAGCAATTTTTTATGGTTTCAGCTAATGTGAATTTGATAACAAAACTTGTTAATATACTAACTATAC
AAGATTGGCAATATCATGGTGATAATTTGTTATTATCAAATCAAACTATAGTATTTACTGGAACTTTTGCTACTGTTTCA
CGGTCTGAAATTAAAGTACAAGCAGAGAAATTAGGAGCAAAGGTAGGTACTCAAGTATCTAATAATACTGATTTACTGGT
AGTAGGTAACAAAGCTGGAAACAAGCTACAGAAAGCTCAGCAACTAGGAATCAAAATTATTAATGAAGAAGAATGGATCA
AAATGGTTAATGAATTATGA

Upstream 100 bases:

>100_bases
TCTCAGAGTAAAGAATGAAACAAAGTGTATAATGTAAATGTTAAAAATTGCAAAAATTGGAGTATTATAGTTTATAGTTT
ATTAAACATCATACATAAAA

Downstream 100 bases:

>100_bases
AGCATAATGAACAAAGGTTGAAGCAATTAAGGCAAAAATTTTTAGAATTTGGTATCAGTGGATACATTATTCCTTCCAGT
AATGAATATCAAAGTGAATG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 699; Mature: 699

Protein sequence:

>699_residues
MNKLDISTINIEQLTSDHAKKIVDFLALELQKYDQAYYSDNNPLVTDAQYDVLKNLNNKIVAKFPHLALVNDHSKKVGFT
PSAQFSKVVHLKPMLSLANGFCVEDISNFITKIQNFLKIDHCPKIVCEYKIDGLSFNARYEYGVLTLASTRGDGQIGENI
TENLKTIQSFPQTLPITDKVFEVRGEIYITNNDFRVLNIQQQKLGKALFSNPRNAAAGSIRQLDPAITAQRPLKYFVYAL
GEVTNHHFASTQFELLQKLSQLKFSVNTDYILSDNLHSMIEFYNNVATERNNLAFEIDGVVYKVNDFALQERLGATSTSP
RFAIAYKFPALIGRTKITNITLQVGKTGAVTPVAWLIPINIAGVTISRATLHNKQEIESKDIRIGDYVFLHRAGDVIPKI
NGVDLSARDTQSSERFIFPTTCPSCNQNLVVNEGETVARCGNSLACQAQIYERICHFVSKDALNIDGLGKQRIQFLLDNK
YIVNIVDIFLLEENNKFLYSNKLEDIAGWGIKSVNKLFQNINQAKNVILDKFIYALAIKHVGKYSAKLLAKEFKTAKNFI
NQSLKLANNATEIYEKLRNIEGVGVKTADQLKQFFMVSANVNLITKLVNILTIQDWQYHGDNLLLSNQTIVFTGTFATVS
RSEIKVQAEKLGAKVGTQVSNNTDLLVVGNKAGNKLQKAQQLGIKIINEEEWIKMVNEL

Sequences:

>Translated_699_residues
MNKLDISTINIEQLTSDHAKKIVDFLALELQKYDQAYYSDNNPLVTDAQYDVLKNLNNKIVAKFPHLALVNDHSKKVGFT
PSAQFSKVVHLKPMLSLANGFCVEDISNFITKIQNFLKIDHCPKIVCEYKIDGLSFNARYEYGVLTLASTRGDGQIGENI
TENLKTIQSFPQTLPITDKVFEVRGEIYITNNDFRVLNIQQQKLGKALFSNPRNAAAGSIRQLDPAITAQRPLKYFVYAL
GEVTNHHFASTQFELLQKLSQLKFSVNTDYILSDNLHSMIEFYNNVATERNNLAFEIDGVVYKVNDFALQERLGATSTSP
RFAIAYKFPALIGRTKITNITLQVGKTGAVTPVAWLIPINIAGVTISRATLHNKQEIESKDIRIGDYVFLHRAGDVIPKI
NGVDLSARDTQSSERFIFPTTCPSCNQNLVVNEGETVARCGNSLACQAQIYERICHFVSKDALNIDGLGKQRIQFLLDNK
YIVNIVDIFLLEENNKFLYSNKLEDIAGWGIKSVNKLFQNINQAKNVILDKFIYALAIKHVGKYSAKLLAKEFKTAKNFI
NQSLKLANNATEIYEKLRNIEGVGVKTADQLKQFFMVSANVNLITKLVNILTIQDWQYHGDNLLLSNQTIVFTGTFATVS
RSEIKVQAEKLGAKVGTQVSNNTDLLVVGNKAGNKLQKAQQLGIKIINEEEWIKMVNEL
>Mature_699_residues
MNKLDISTINIEQLTSDHAKKIVDFLALELQKYDQAYYSDNNPLVTDAQYDVLKNLNNKIVAKFPHLALVNDHSKKVGFT
PSAQFSKVVHLKPMLSLANGFCVEDISNFITKIQNFLKIDHCPKIVCEYKIDGLSFNARYEYGVLTLASTRGDGQIGENI
TENLKTIQSFPQTLPITDKVFEVRGEIYITNNDFRVLNIQQQKLGKALFSNPRNAAAGSIRQLDPAITAQRPLKYFVYAL
GEVTNHHFASTQFELLQKLSQLKFSVNTDYILSDNLHSMIEFYNNVATERNNLAFEIDGVVYKVNDFALQERLGATSTSP
RFAIAYKFPALIGRTKITNITLQVGKTGAVTPVAWLIPINIAGVTISRATLHNKQEIESKDIRIGDYVFLHRAGDVIPKI
NGVDLSARDTQSSERFIFPTTCPSCNQNLVVNEGETVARCGNSLACQAQIYERICHFVSKDALNIDGLGKQRIQFLLDNK
YIVNIVDIFLLEENNKFLYSNKLEDIAGWGIKSVNKLFQNINQAKNVILDKFIYALAIKHVGKYSAKLLAKEFKTAKNFI
NQSLKLANNATEIYEKLRNIEGVGVKTADQLKQFFMVSANVNLITKLVNILTIQDWQYHGDNLLLSNQTIVFTGTFATVS
RSEIKVQAEKLGAKVGTQVSNNTDLLVVGNKAGNKLQKAQQLGIKIINEEEWIKMVNEL

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=681, Percent_Identity=37.4449339207048, Blast_Score=468, Evalue=1e-133,
Organism=Escherichia coli, GI87082305, Length=540, Percent_Identity=22.4074074074074, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_ORITI (B3CVK1)

Other databases:

- EMBL:   AP008981
- RefSeq:   YP_001938632.1
- GeneID:   6337670
- GenomeReviews:   AP008981_GR
- KEGG:   ott:OTT_1940
- HOGENOM:   HBG620317
- OMA:   FHELKAL
- ProtClustDB:   PRK07956
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 78466; Mature: 78466

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 130-130 BINDING 128-128 BINDING 151-151 BINDING 186-186 BINDING 303-303 BINDING 327-327

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKLDISTINIEQLTSDHAKKIVDFLALELQKYDQAYYSDNNPLVTDAQYDVLKNLNNKI
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHCCCCCE
VAKFPHLALVNDHSKKVGFTPSAQFSKVVHLKPMLSLANGFCVEDISNFITKIQNFLKID
EEECCCEEEECCCCCCCCCCCCHHHHHEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
HCPKIVCEYKIDGLSFNARYEYGVLTLASTRGDGQIGENITENLKTIQSFPQTLPITDKV
CCCCEEEEEEECCEEECCEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHH
FEVRGEIYITNNDFRVLNIQQQKLGKALFSNPRNAAAGSIRQLDPAITAQRPLKYFVYAL
HEECCEEEEECCCEEEEEECHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHH
GEVTNHHFASTQFELLQKLSQLKFSVNTDYILSDNLHSMIEFYNNVATERNNLAFEIDGV
HHHHCCCCCHHHHHHHHHHHHCEEECCCCEEECCCHHHHHHHHHHHHCCCCCEEEEECCE
VYKVNDFALQERLGATSTSPRFAIAYKFPALIGRTKITNITLQVGKTGAVTPVAWLIPIN
EEEEHHHHHHHHHCCCCCCCCEEEEEECHHHHCCEEEEEEEEEECCCCCCCCEEEEEEEE
IAGVTISRATLHNKQEIESKDIRIGDYVFLHRAGDVIPKINGVDLSARDTQSSERFIFPT
EECEEEEHHHHCCHHHCCCCCCEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEC
TCPSCNQNLVVNEGETVARCGNSLACQAQIYERICHFVSKDALNIDGLGKQRIQFLLDNK
CCCCCCCCEEEECCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCC
YIVNIVDIFLLEENNKFLYSNKLEDIAGWGIKSVNKLFQNINQAKNVILDKFIYALAIKH
EEEEEEEEEEEECCCCEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGKYSAKLLAKEFKTAKNFINQSLKLANNATEIYEKLRNIEGVGVKTADQLKQFFMVSAN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHEEECC
VNLITKLVNILTIQDWQYHGDNLLLSNQTIVFTGTFATVSRSEIKVQAEKLGAKVGTQVS
HHHHHHHHHHHEEECCEECCCEEEEECCEEEEEECCCEECHHHHEEEHHHHCHHHCCCCC
NNTDLLVVGNKAGNKLQKAQQLGIKIINEEEWIKMVNEL
CCCCEEEEECCCCHHHHHHHHCCCEEECHHHHHHHHHCC
>Mature Secondary Structure
MNKLDISTINIEQLTSDHAKKIVDFLALELQKYDQAYYSDNNPLVTDAQYDVLKNLNNKI
CCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCHHHHHHCCCCCE
VAKFPHLALVNDHSKKVGFTPSAQFSKVVHLKPMLSLANGFCVEDISNFITKIQNFLKID
EEECCCEEEECCCCCCCCCCCCHHHHHEEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHC
HCPKIVCEYKIDGLSFNARYEYGVLTLASTRGDGQIGENITENLKTIQSFPQTLPITDKV
CCCCEEEEEEECCEEECCEEEEEEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCHHH
FEVRGEIYITNNDFRVLNIQQQKLGKALFSNPRNAAAGSIRQLDPAITAQRPLKYFVYAL
HEECCEEEEECCCEEEEEECHHHHHHHHHCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHH
GEVTNHHFASTQFELLQKLSQLKFSVNTDYILSDNLHSMIEFYNNVATERNNLAFEIDGV
HHHHCCCCCHHHHHHHHHHHHCEEECCCCEEECCCHHHHHHHHHHHHCCCCCEEEEECCE
VYKVNDFALQERLGATSTSPRFAIAYKFPALIGRTKITNITLQVGKTGAVTPVAWLIPIN
EEEEHHHHHHHHHCCCCCCCCEEEEEECHHHHCCEEEEEEEEEECCCCCCCCEEEEEEEE
IAGVTISRATLHNKQEIESKDIRIGDYVFLHRAGDVIPKINGVDLSARDTQSSERFIFPT
EECEEEEHHHHCCHHHCCCCCCEECCEEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEC
TCPSCNQNLVVNEGETVARCGNSLACQAQIYERICHFVSKDALNIDGLGKQRIQFLLDNK
CCCCCCCCEEEECCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCC
YIVNIVDIFLLEENNKFLYSNKLEDIAGWGIKSVNKLFQNINQAKNVILDKFIYALAIKH
EEEEEEEEEEEECCCCEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGKYSAKLLAKEFKTAKNFINQSLKLANNATEIYEKLRNIEGVGVKTADQLKQFFMVSAN
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHEEECC
VNLITKLVNILTIQDWQYHGDNLLLSNQTIVFTGTFATVSRSEIKVQAEKLGAKVGTQVS
HHHHHHHHHHHEEECCEECCCEEEEECCEEEEEECCCEECHHHHEEEHHHHCHHHCCCCC
NNTDLLVVGNKAGNKLQKAQQLGIKIINEEEWIKMVNEL
CCCCEEEEECCCCHHHHHHHHCCCEEECHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA