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The map label for this gene is traA [H]

Identifier: 189184742

GI number: 189184742

Start: 1869177

End: 1871543

Strand: Reverse

Name: traA [H]

Synonym: OTT_1835

Alternate gene names: 189184742

Gene position: 1871543-1869177 (Counterclockwise)

Preceding gene: 189184743

Following gene: 189184741

Centisome position: 93.16

GC content: 33.92

Gene sequence:

>2367_bases
TTGGGTGGTAAAGCTGTTGACTTGGAGCCTAAATTCAGAACATCGAAAGGTAAGGCGTTTATTATTCCAGAAGCTGAGAT
GATTCATGAAAAAGTGAAGGAAATAATTAATGCATATTTTGCTAAATTAGGCTTATCAAATAGAGTTGATGATATAAGTA
TAGTGCCACAGAAGCATATTGGACCTACTAGAATTAGGAGTTTAATTAATGAAGTAGCAAATGAAAATGAGTTACGTAAA
GAGGCTAATTTAAAAATTATTAAGGATGCTGATGTAATAACGGATTCTATAACACATTACAAATCTATTTTTACTAAGCA
GGATGTTGAAAAAGCCGTGAAAGATATACCAGACCTAACAGCAAGAGAACGGTTAGTTCAACAAGTGCTCAGTTCAAATA
GAGTATTAGAATTGTATCATGAGAATGGTGAAAGTAGCAAATATTTTACTACAACTGAGGTTAGAGACGAGGAAGTAAGA
ATAATAAGAATAGCTAATAAAATCAATGATCAGGTTTATTACAACGATATTTACAATCTTAAAAGCGATATAGAAGGACT
AACAAATGTTAGTGAAGAACAGAAACAAGCTCTAAGGCATATTTTGCTTAGTACTAGTGGAGTTAGAGTCTTAAGAGGAA
GAGCTGGTACAGGAAAATCTCATGTTTTAGCGAAAGCATATGAGCTTGCAACAAATCGTGGACAAAAAGTTATTGGTCTT
GCTCCTACTCATAAAGCGGTATCAGAGCTGAGGAGCAAAGGCTATACAGAGGTCTATACAGTAAAAGGATTTTTGTATAA
TCGAAAAAAAATTTTTATGCAAGGCAGCTTAATAGTAGTAGATGAAGCTGGAATGGTAGGTACTAAAGCTTATGCAGAGC
TGTTTAGAGTAGTTAGAAACAATAATTGTCAACTGATACTTGCTGGAGATGAAAAGCAGTTAGCTTCTATAGAAAGAGGC
GGAATGTTTGAGATGCTGAGTAATATTTTCGGTTCACATGTTTTAGTAAATATTCGAAGACAAAGTGAAAACTGGAGCAG
AGAAGCAGCAACAAAATTTGCTGAGAGTAATATTTTAAGCGGTATAACATTACTGAAACAAAATAACTGCGTTAAGTTTG
ATAATACGTTGCAGGACTCAATGAGTAAGCTAGTATATAACTGGAGTCTAAGCAAGTTTAAGCCACATGAAAAATTGGTA
ATTACAGTACGTAATAAAGATGTCGACATTCTTAATTCAAGTATTAGATCTTTGCTAAAAGCCAATGGCACGCTACAAGG
TCCAGAATATAGGCGTTCAATTGCTGGAAGGAAAGAGTCGTATATGGCAGGAGATAGAATTGTCTTTCAAGCAAGCAATA
AGGATTTACAAATACAAAACAGTGATTTTGCAACTTTAACTTCGGTTAATAAAAATGAATTTGTAGCTAAGACAGACGCA
GGAAAAGAGGTGAGTTTTGATCCTAGTAAAATACAATTTAAACATGGCTATGCAAGTACTGTTTATAAGGCGCAGGGAGC
TTCTATAAAAGATGTATACGTTTTGCATAATGGAGTAAGTAATATAAGCAGCTCATACGTAGCTATGACAAGGCATATAG
AGAAATTACAGCTATATTGCAATAAGGAAGCTACTAAAAGCATTAACAGCTTAATAAATCAGCTCAGCAGACCAAATGAG
AAATCAGCTAGCATAACTCTGAAAACTGCTCATGATTTGGAGAAAGAACAGACAAAGACAACTGTTTTTAGTAAAATCGA
AAACTGGTTTAAGTCCGTAATCAATGATATCAATGATAGATCTCATGTGAATGAAGAATATTATCATTTTACCGTTAAAC
CAGATCAAGAAGCTAAAGTAGAAAGAGTCCAGCAAGAGAATCGCATAAAGCAATGTTCATACAAAGATCTGTCTACTCCA
TTGTTTATGCAAATCAAAGAGCAAAGACAGTACAATTATGATGTAACCCTTTTATCTGCAGAAGGAAAAACGATATCAAG
CTTCCAGGAGATCGGTATTGATAGCAGAATGGTATATAGCTCAAATGTAAATAATTTGAAGTACTATCAACCATTTCAAG
GAGAAAAGATTCTTATAGCTGCAAATAATGATAAACAGAATAAGGAATATATAAGCACTATAAATGAGGCTGCAACAGCA
TTAAAAAGCAAAGGAGCAATTACTAGCATTGTAATTCCTTCAGAAGGCGAAGATTTTAACGAAATGCTAAAAAACAAAGG
ATCCATAGCTGTTAAGGCCTTTGTGATACCTGAAATCATGAAATTAGTTAATACTCAGAACGTAGAAACACATCCTGAAC
AAATTGTAAAAGCTGATATAGCTCTAAAAATTGGAATTAGAAGGTAG

Upstream 100 bases:

>100_bases
TGGAGTACAGATAGACATTCATAAGCCTCATATATGAGATAAAAACTGGCATGCGCATATATTGGTTACTACAAGAAGAT
TTAAAGAGAATGGCGAAGAA

Downstream 100 bases:

>100_bases
TGCTGAAGATTTGAAATTGAATATAAGCTGTTCATTTACGGAGCGAATTACAAAATGAGCTATACATGATAGTTGAGATG
GACTTTTGTAATTCGCATTA

Product: putative conjugative transfer protein TraA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 788; Mature: 787

Protein sequence:

>788_residues
MGGKAVDLEPKFRTSKGKAFIIPEAEMIHEKVKEIINAYFAKLGLSNRVDDISIVPQKHIGPTRIRSLINEVANENELRK
EANLKIIKDADVITDSITHYKSIFTKQDVEKAVKDIPDLTARERLVQQVLSSNRVLELYHENGESSKYFTTTEVRDEEVR
IIRIANKINDQVYYNDIYNLKSDIEGLTNVSEEQKQALRHILLSTSGVRVLRGRAGTGKSHVLAKAYELATNRGQKVIGL
APTHKAVSELRSKGYTEVYTVKGFLYNRKKIFMQGSLIVVDEAGMVGTKAYAELFRVVRNNNCQLILAGDEKQLASIERG
GMFEMLSNIFGSHVLVNIRRQSENWSREAATKFAESNILSGITLLKQNNCVKFDNTLQDSMSKLVYNWSLSKFKPHEKLV
ITVRNKDVDILNSSIRSLLKANGTLQGPEYRRSIAGRKESYMAGDRIVFQASNKDLQIQNSDFATLTSVNKNEFVAKTDA
GKEVSFDPSKIQFKHGYASTVYKAQGASIKDVYVLHNGVSNISSSYVAMTRHIEKLQLYCNKEATKSINSLINQLSRPNE
KSASITLKTAHDLEKEQTKTTVFSKIENWFKSVINDINDRSHVNEEYYHFTVKPDQEAKVERVQQENRIKQCSYKDLSTP
LFMQIKEQRQYNYDVTLLSAEGKTISSFQEIGIDSRMVYSSNVNNLKYYQPFQGEKILIAANNDKQNKEYISTINEAATA
LKSKGAITSIVIPSEGEDFNEMLKNKGSIAVKAFVIPEIMKLVNTQNVETHPEQIVKADIALKIGIRR

Sequences:

>Translated_788_residues
MGGKAVDLEPKFRTSKGKAFIIPEAEMIHEKVKEIINAYFAKLGLSNRVDDISIVPQKHIGPTRIRSLINEVANENELRK
EANLKIIKDADVITDSITHYKSIFTKQDVEKAVKDIPDLTARERLVQQVLSSNRVLELYHENGESSKYFTTTEVRDEEVR
IIRIANKINDQVYYNDIYNLKSDIEGLTNVSEEQKQALRHILLSTSGVRVLRGRAGTGKSHVLAKAYELATNRGQKVIGL
APTHKAVSELRSKGYTEVYTVKGFLYNRKKIFMQGSLIVVDEAGMVGTKAYAELFRVVRNNNCQLILAGDEKQLASIERG
GMFEMLSNIFGSHVLVNIRRQSENWSREAATKFAESNILSGITLLKQNNCVKFDNTLQDSMSKLVYNWSLSKFKPHEKLV
ITVRNKDVDILNSSIRSLLKANGTLQGPEYRRSIAGRKESYMAGDRIVFQASNKDLQIQNSDFATLTSVNKNEFVAKTDA
GKEVSFDPSKIQFKHGYASTVYKAQGASIKDVYVLHNGVSNISSSYVAMTRHIEKLQLYCNKEATKSINSLINQLSRPNE
KSASITLKTAHDLEKEQTKTTVFSKIENWFKSVINDINDRSHVNEEYYHFTVKPDQEAKVERVQQENRIKQCSYKDLSTP
LFMQIKEQRQYNYDVTLLSAEGKTISSFQEIGIDSRMVYSSNVNNLKYYQPFQGEKILIAANNDKQNKEYISTINEAATA
LKSKGAITSIVIPSEGEDFNEMLKNKGSIAVKAFVIPEIMKLVNTQNVETHPEQIVKADIALKIGIRR
>Mature_787_residues
GGKAVDLEPKFRTSKGKAFIIPEAEMIHEKVKEIINAYFAKLGLSNRVDDISIVPQKHIGPTRIRSLINEVANENELRKE
ANLKIIKDADVITDSITHYKSIFTKQDVEKAVKDIPDLTARERLVQQVLSSNRVLELYHENGESSKYFTTTEVRDEEVRI
IRIANKINDQVYYNDIYNLKSDIEGLTNVSEEQKQALRHILLSTSGVRVLRGRAGTGKSHVLAKAYELATNRGQKVIGLA
PTHKAVSELRSKGYTEVYTVKGFLYNRKKIFMQGSLIVVDEAGMVGTKAYAELFRVVRNNNCQLILAGDEKQLASIERGG
MFEMLSNIFGSHVLVNIRRQSENWSREAATKFAESNILSGITLLKQNNCVKFDNTLQDSMSKLVYNWSLSKFKPHEKLVI
TVRNKDVDILNSSIRSLLKANGTLQGPEYRRSIAGRKESYMAGDRIVFQASNKDLQIQNSDFATLTSVNKNEFVAKTDAG
KEVSFDPSKIQFKHGYASTVYKAQGASIKDVYVLHNGVSNISSSYVAMTRHIEKLQLYCNKEATKSINSLINQLSRPNEK
SASITLKTAHDLEKEQTKTTVFSKIENWFKSVINDINDRSHVNEEYYHFTVKPDQEAKVERVQQENRIKQCSYKDLSTPL
FMQIKEQRQYNYDVTLLSAEGKTISSFQEIGIDSRMVYSSNVNNLKYYQPFQGEKILIAANNDKQNKEYISTINEAATAL
KSKGAITSIVIPSEGEDFNEMLKNKGSIAVKAFVIPEIMKLVNTQNVETHPEQIVKADIALKIGIRR

Specific function: traI Has Been Identified As DNA Helicase I And It Also Has An Additional Activity Of Site-Specific Nicking At Orit. DNA Helicase I Is A Potent DNA-Dependent ATPase. [C]

COG id: COG0507

COG function: function code L; ATP-dependent exoDNAse (exonuclease V), alpha subunit - helicase superfamily I member

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mobA/mobL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000606
- InterPro:   IPR005053
- InterPro:   IPR014136 [H]

Pfam domain/function: PF03389 MobA_MobL; PF01443 Viral_helicase1 [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 89093; Mature: 88961

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGKAVDLEPKFRTSKGKAFIIPEAEMIHEKVKEIINAYFAKLGLSNRVDDISIVPQKHI
CCCCEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCC
GPTRIRSLINEVANENELRKEANLKIIKDADVITDSITHYKSIFTKQDVEKAVKDIPDLT
CHHHHHHHHHHHHCHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ARERLVQQVLSSNRVLELYHENGESSKYFTTTEVRDEEVRIIRIANKINDQVYYNDIYNL
HHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCCCEEEEEEEHHHCCCEEEHHHHHH
KSDIEGLTNVSEEQKQALRHILLSTSGVRVLRGRAGTGKSHVLAKAYELATNRGQKVIGL
HHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEE
APTHKAVSELRSKGYTEVYTVKGFLYNRKKIFMQGSLIVVDEAGMVGTKAYAELFRVVRN
CCCHHHHHHHHHCCCCEEEEEEEEEECCEEEEEECCEEEEECCCCCCHHHHHHHHHHHHC
NNCQLILAGDEKQLASIERGGMFEMLSNIFGSHVLVNIRRQSENWSREAATKFAESNILS
CCCEEEEECCHHHHHHHHCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHH
GITLLKQNNCVKFDNTLQDSMSKLVYNWSLSKFKPHEKLVITVRNKDVDILNSSIRSLLK
HHHEEECCCCEECCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHH
ANGTLQGPEYRRSIAGRKESYMAGDRIVFQASNKDLQIQNSDFATLTSVNKNEFVAKTDA
HCCCCCCHHHHHHHCCCCHHHCCCCEEEEEECCCCEEEECCCCEEEEECCCCCEEEECCC
GKEVSFDPSKIQFKHGYASTVYKAQGASIKDVYVLHNGVSNISSSYVAMTRHIEKLQLYC
CCCCCCCCCCEEEECCCHHHEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
NKEATKSINSLINQLSRPNEKSASITLKTAHDLEKEQTKTTVFSKIENWFKSVINDINDR
CCHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SHVNEEYYHFTVKPDQEAKVERVQQENRIKQCSYKDLSTPLFMQIKEQRQYNYDVTLLSA
HCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEC
EGKTISSFQEIGIDSRMVYSSNVNNLKYYQPFQGEKILIAANNDKQNKEYISTINEAATA
CCCCHHHHHHCCCCCEEEEECCCCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
LKSKGAITSIVIPSEGEDFNEMLKNKGSIAVKAFVIPEIMKLVNTQNVETHPEQIVKADI
HHCCCCEEEEEECCCCCCHHHHHHCCCCEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHE
ALKIGIRR
EEEEECCC
>Mature Secondary Structure 
GGKAVDLEPKFRTSKGKAFIIPEAEMIHEKVKEIINAYFAKLGLSNRVDDISIVPQKHI
CCCEECCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCC
GPTRIRSLINEVANENELRKEANLKIIKDADVITDSITHYKSIFTKQDVEKAVKDIPDLT
CHHHHHHHHHHHHCHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ARERLVQQVLSSNRVLELYHENGESSKYFTTTEVRDEEVRIIRIANKINDQVYYNDIYNL
HHHHHHHHHHCCCCEEEEEECCCCCCCEEEEECCCCCCEEEEEEEHHHCCCEEEHHHHHH
KSDIEGLTNVSEEQKQALRHILLSTSGVRVLRGRAGTGKSHVLAKAYELATNRGQKVIGL
HHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEE
APTHKAVSELRSKGYTEVYTVKGFLYNRKKIFMQGSLIVVDEAGMVGTKAYAELFRVVRN
CCCHHHHHHHHHCCCCEEEEEEEEEECCEEEEEECCEEEEECCCCCCHHHHHHHHHHHHC
NNCQLILAGDEKQLASIERGGMFEMLSNIFGSHVLVNIRRQSENWSREAATKFAESNILS
CCCEEEEECCHHHHHHHHCCCHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHHHHHH
GITLLKQNNCVKFDNTLQDSMSKLVYNWSLSKFKPHEKLVITVRNKDVDILNSSIRSLLK
HHHEEECCCCEECCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHH
ANGTLQGPEYRRSIAGRKESYMAGDRIVFQASNKDLQIQNSDFATLTSVNKNEFVAKTDA
HCCCCCCHHHHHHHCCCCHHHCCCCEEEEEECCCCEEEECCCCEEEEECCCCCEEEECCC
GKEVSFDPSKIQFKHGYASTVYKAQGASIKDVYVLHNGVSNISSSYVAMTRHIEKLQLYC
CCCCCCCCCCEEEECCCHHHEEECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHH
NKEATKSINSLINQLSRPNEKSASITLKTAHDLEKEQTKTTVFSKIENWFKSVINDINDR
CCHHHHHHHHHHHHHCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
SHVNEEYYHFTVKPDQEAKVERVQQENRIKQCSYKDLSTPLFMQIKEQRQYNYDVTLLSA
HCCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEEEEC
EGKTISSFQEIGIDSRMVYSSNVNNLKYYQPFQGEKILIAANNDKQNKEYISTINEAATA
CCCCHHHHHHCCCCCEEEEECCCCCCEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
LKSKGAITSIVIPSEGEDFNEMLKNKGSIAVKAFVIPEIMKLVNTQNVETHPEQIVKADI
HHCCCCEEEEEECCCCCCHHHHHHCCCCEEEEEHHHHHHHHHHCCCCCCCCHHHHHHHHE
ALKIGIRR
EEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on acid anhydrides; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8763953; 11743193; 11743194 [H]