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The map label for this gene is tyrS [H]

Identifier: 189184678

GI number: 189184678

Start: 1796361

End: 1797602

Strand: Reverse

Name: tyrS [H]

Synonym: OTT_1771

Alternate gene names: 189184678

Gene position: 1797602-1796361 (Counterclockwise)

Preceding gene: 189184683

Following gene: 189184677

Centisome position: 89.48

GC content: 30.11

Gene sequence:

>1242_bases
ATGCATTTTATAAACGAATTCATTAATCGAGGTTATTTTTATCAATCTACTGACCTTACAAGGCTAACACAAATTAGTAA
TAGTAGCCAGATAGTAGCTTATATTGGTTTTGACTGTACAGCTCAGTCTTTGCACGTTGGAAATCTTATGCAGATAATGA
TATTAAGGCTATTACAGCAATGCGGCCATAAGTCTATAGTTGTTATTGGAGGAGCTACTACTAAAATTGGTGATCCATCT
GAAAAAGATAAATTGCGTAGAATTATAACTAATGATGAAATACAGCAAAATATATCTGGAATTAAAAGATCACTAAAAAA
ATTTATTAAATTTGATGAAACAAAAAATGACGTATTACTATTAAACAATCAGGAATGGCTAGATTCTATCAACTATATTA
ATTTTCTTCGGGACTATGGTAGAGCATTTTCAGTAAACAAGATGTTAACCATGAATTCTGTTAAATCAAGGCTAGAGCGG
CACACTCCTTTAAGCTTTTTAGAATTTAACTATATGCTGTTACAAGCATATGATTTTTACTATCTAAATAAATATTACAA
CTGCAATCTTCAAATTGGAGGTAGTGACCAATGGGGCAATATCACTATGGGCGTAGATTTAGTTAAAAAATTAAGTAATA
ATGAAGTATTTGGCTTAACAACTCCATTAATCACAAACTCATCTGGAGAAAAAATGGGAAAAACAGCTGATGGAGCTGTT
TGGTTAAATGAAGATATGTGTTCTCCATACAATTATTTCCAATATTGGAGAAATATAGAAGACAATGATGTAATACGCTT
TGCTAAGTTGTATGGTGAATTTTCTGAAGTAGAGCTATCTAAGCTTACAGAATTATTCTTCAATAATGTCAACGAAGCTA
AAAAGCAAATCGCTTATAAAATCACTTTATTATGCCATGGAAGGGATGAAGCAAATAAAGCACTTAATACCGCAATTCAA
ATGTTTGAGCATAAAAAAGCTGACGAAAATTTGCCAACATTTACTATAAAAGACTGCAATAATTTAAAGGTTGGTATACC
AATCACTGAATTATTAGTAACAATTGGGTTAGCTAAAACTAAATCTGAGGGAAAAAGATTAATTCAAGGTAATGGAATAA
GAATCAACAATATAAAAGTTAATAACATAAACTTAGTTGTGCAATTGCATGATTTTATAGATCAAGTGATTACAGTTTCA
TTAGGCAAAAAATGCCATATTTTAGTTAAAATAGCTAAATAA

Upstream 100 bases:

>100_bases
TTTATCATAATTTGGTGTGTTGGGTAATTTTATTGACTGATTCACTATCTATCTTTATTATTTTATCTAGTAGTAAGGTT
TACATAACAAAAATAAAATC

Downstream 100 bases:

>100_bases
CATCTTGTTTTAAACATAGTTAAAATCATTGATAGTCAGACAGAAGTAAGATAAAACAAAAGTTTGTATAAAATAATTCA
AACTTGCAAAAGAAACAGAT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MHFINEFINRGYFYQSTDLTRLTQISNSSQIVAYIGFDCTAQSLHVGNLMQIMILRLLQQCGHKSIVVIGGATTKIGDPS
EKDKLRRIITNDEIQQNISGIKRSLKKFIKFDETKNDVLLLNNQEWLDSINYINFLRDYGRAFSVNKMLTMNSVKSRLER
HTPLSFLEFNYMLLQAYDFYYLNKYYNCNLQIGGSDQWGNITMGVDLVKKLSNNEVFGLTTPLITNSSGEKMGKTADGAV
WLNEDMCSPYNYFQYWRNIEDNDVIRFAKLYGEFSEVELSKLTELFFNNVNEAKKQIAYKITLLCHGRDEANKALNTAIQ
MFEHKKADENLPTFTIKDCNNLKVGIPITELLVTIGLAKTKSEGKRLIQGNGIRINNIKVNNINLVVQLHDFIDQVITVS
LGKKCHILVKIAK

Sequences:

>Translated_413_residues
MHFINEFINRGYFYQSTDLTRLTQISNSSQIVAYIGFDCTAQSLHVGNLMQIMILRLLQQCGHKSIVVIGGATTKIGDPS
EKDKLRRIITNDEIQQNISGIKRSLKKFIKFDETKNDVLLLNNQEWLDSINYINFLRDYGRAFSVNKMLTMNSVKSRLER
HTPLSFLEFNYMLLQAYDFYYLNKYYNCNLQIGGSDQWGNITMGVDLVKKLSNNEVFGLTTPLITNSSGEKMGKTADGAV
WLNEDMCSPYNYFQYWRNIEDNDVIRFAKLYGEFSEVELSKLTELFFNNVNEAKKQIAYKITLLCHGRDEANKALNTAIQ
MFEHKKADENLPTFTIKDCNNLKVGIPITELLVTIGLAKTKSEGKRLIQGNGIRINNIKVNNINLVVQLHDFIDQVITVS
LGKKCHILVKIAK
>Mature_413_residues
MHFINEFINRGYFYQSTDLTRLTQISNSSQIVAYIGFDCTAQSLHVGNLMQIMILRLLQQCGHKSIVVIGGATTKIGDPS
EKDKLRRIITNDEIQQNISGIKRSLKKFIKFDETKNDVLLLNNQEWLDSINYINFLRDYGRAFSVNKMLTMNSVKSRLER
HTPLSFLEFNYMLLQAYDFYYLNKYYNCNLQIGGSDQWGNITMGVDLVKKLSNNEVFGLTTPLITNSSGEKMGKTADGAV
WLNEDMCSPYNYFQYWRNIEDNDVIRFAKLYGEFSEVELSKLTELFFNNVNEAKKQIAYKITLLCHGRDEANKALNTAIQ
MFEHKKADENLPTFTIKDCNNLKVGIPITELLVTIGLAKTKSEGKRLIQGNGIRINNIKVNNINLVVQLHDFIDQVITVS
LGKKCHILVKIAK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=399, Percent_Identity=32.3308270676692, Blast_Score=214, Evalue=1e-55,
Organism=Escherichia coli, GI1787925, Length=422, Percent_Identity=37.6777251184834, Blast_Score=281, Evalue=7e-77,
Organism=Caenorhabditis elegans, GI25145802, Length=303, Percent_Identity=34.6534653465347, Blast_Score=181, Evalue=4e-46,
Organism=Caenorhabditis elegans, GI25145805, Length=217, Percent_Identity=33.6405529953917, Blast_Score=134, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6325160, Length=300, Percent_Identity=37.6666666666667, Blast_Score=209, Evalue=9e-55,
Organism=Drosophila melanogaster, GI20130383, Length=436, Percent_Identity=33.0275229357798, Blast_Score=232, Evalue=3e-61,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 47311; Mature: 47311

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFINEFINRGYFYQSTDLTRLTQISNSSQIVAYIGFDCTAQSLHVGNLMQIMILRLLQQ
CCHHHHHHHCCEEEECCCCHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
CGHKSIVVIGGATTKIGDPSEKDKLRRIITNDEIQQNISGIKRSLKKFIKFDETKNDVLL
CCCCEEEEECCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
LNNQEWLDSINYINFLRDYGRAFSVNKMLTMNSVKSRLERHTPLSFLEFNYMLLQAYDFY
ECCHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHE
YLNKYYNCNLQIGGSDQWGNITMGVDLVKKLSNNEVFGLTTPLITNSSGEKMGKTADGAV
EEEEEEEEEEEECCCCCCCCEEEHHHHHHHHCCCCEEEEECCEEECCCCCCCCCCCCCEE
WLNEDMCSPYNYFQYWRNIEDNDVIRFAKLYGEFSEVELSKLTELFFNNVNEAKKQIAYK
EECCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHEEE
ITLLCHGRDEANKALNTAIQMFEHKKADENLPTFTIKDCNNLKVGIPITELLVTIGLAKT
EEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEECCCHHHHHHHHHHHHC
KSEGKRLIQGNGIRINNIKVNNINLVVQLHDFIDQVITVSLGKKCHILVKIAK
HHCCCCEEECCCEEEEEEEEECEEEEEEHHHHHHHHHHHHCCCCEEEEEEEEC
>Mature Secondary Structure
MHFINEFINRGYFYQSTDLTRLTQISNSSQIVAYIGFDCTAQSLHVGNLMQIMILRLLQQ
CCHHHHHHHCCEEEECCCCHHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
CGHKSIVVIGGATTKIGDPSEKDKLRRIITNDEIQQNISGIKRSLKKFIKFDETKNDVLL
CCCCEEEEECCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
LNNQEWLDSINYINFLRDYGRAFSVNKMLTMNSVKSRLERHTPLSFLEFNYMLLQAYDFY
ECCHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHE
YLNKYYNCNLQIGGSDQWGNITMGVDLVKKLSNNEVFGLTTPLITNSSGEKMGKTADGAV
EEEEEEEEEEEECCCCCCCCEEEHHHHHHHHCCCCEEEEECCEEECCCCCCCCCCCCCEE
WLNEDMCSPYNYFQYWRNIEDNDVIRFAKLYGEFSEVELSKLTELFFNNVNEAKKQIAYK
EECCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHEEE
ITLLCHGRDEANKALNTAIQMFEHKKADENLPTFTIKDCNNLKVGIPITELLVTIGLAKT
EEEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCEECCCHHHHHHHHHHHHC
KSEGKRLIQGNGIRINNIKVNNINLVVQLHDFIDQVITVSLGKKCHILVKIAK
HHCCCCEEECCCEEEEEEEEECEEEEEEHHHHHHHHHHHHCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA