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The map label for this gene is traA [H]

Identifier: 189184585

GI number: 189184585

Start: 1702951

End: 1704699

Strand: Reverse

Name: traA [H]

Synonym: OTT_1678

Alternate gene names: 189184585

Gene position: 1704699-1702951 (Counterclockwise)

Preceding gene: 189184586

Following gene: 189184584

Centisome position: 84.85

GC content: 33.45

Gene sequence:

>1749_bases
TTGCTTAGCACTAGTGGAGTTAGAGTCTTGAGAGGAAGAGCTGGTACAGGAAAATCTTATGTTTTAATAAAAGCGCATAA
GCTCGCAACAAATCGTAGACAAAAAGTTATTGGTCTTGCTCCTACTCATAAGGCAGTATCAGAGCTGAGGAGTAAAGGTT
ATACAGAGGTCTATACAGTAAAAGGATTTTTGTATAATCGAAAAAAAAATTTTATGCAAGGCAGCTTAATAGTAGTAGAT
GAAGCTGGAATGGTAGGTACTAAAGCTTATGCAGAGCTGTTTAGAGTAGTTAGAAACAATTATTGTCAACTGATACTCGC
TGGAGATGAAAAACAGCTAGCTTCAATAGAAAGAGGCGGAATGTTTGAGATGCTGAGTAATAATTTCGGTTCACATGTTT
TAATAGATATTCGCAGACAAAGTGAAAACTGGAGCAGAGAAGCAGCAACAAAGTTTGCTGAGAGTAATATTTTAAGCGGT
ATAACCTTAATGAGACAAAATAAATGTGTTAAGTTTGATAATACATTACAGGATTCAATTAGTAAGTTGATTTATGATTG
GAGTCTAAGCAAGTTTAAGCTACATGAAAAATTGGTAATTACAGTACGTAATAAAGATGTCGATATTCTTAATTCAAGTA
TTAGATCTTTGTTAAAAGCTAATGGCACGCTACAAGGCACAGAATATAGGCGTTCAATTGCTGGAAGGAAAGAGTCCTAT
ATGGCAGGAGATAGAATCGTATTTCAAAAAAGCGATAAGGATTTACAAATACAAAACAGTGAATTTGCAACTTTAACTTC
TGTTAATAAAAATGAATTTGTAGCTAAAACAGATGCAGGAAAAGAGGTGAGTTTTGATCCTAGTAAAATACAATTTAAAC
ATGGCTATGCAAGTACTATTTATAAGGCTCAGGGAGCTTCTATAAAAGATGTATACGTTTTGCATAATGGAGTAAGTAAT
ATAAGCAGCTCATATGTAGCTATGACAAGGCATATAGAGAAGTTGCAGCTATATTGCAATAAGAAAGCTACTGTAAGCAT
TAAAAGTTTAATAAATCAGCTCAGCAGACCAAATGAGAAATCAGCTAGCATAACTCTAAAAACTGCTCATGATTTGGAGA
AAGAACGGACAAAGACAACTGTTTTTAGTAAAGTTTTTAGTAAAGTTGAAAACTGGTTTAAGTCTATAATCAATGATATC
AATGATAGATCTCATGTGAATGAAGAATATTATCATTTTACCGCTAAACCAGAGGAAGAAGCTAAAGTAGAAAAAGTCCA
GCTCAAAGATATTTCTACTCCATTATTTATGCACATCAAAGAACAAAGACAGTATGATTATGATGTAACCATTCTGTCAG
CAGAAGGAAAAACGATATCAAGTTTTCAGGAAGCTGGCATTGATAGCAGAATGGTATATAGCTCAAATGTTAATAATTTG
AAGTACTATCAACCATTTCAAGGAGAAAAGATTCTTATAGCTGCAAATAATGATAAACAGAATAAAGAGTATGCAAGTAC
TATAAACGAGGCTGCAAAAGCATTAAAAAGCAAAGGAGCAATCACTAGCATCGTAGTTCCTTCAGAAGGTGAAGATTTTA
ACGAAATGCTAAAAAATAAAGGAGCCACAGCTGTTAAGGAGCTTATGATACCTGAAATCATGAAGTTAATTAATACTCAG
AACGTAAAAACAGAGTCTGAACAAGTGGTGAAAACTGATATAGCTCAAAAAATTGTAATTAGGAGATAG

Upstream 100 bases:

>100_bases
GAATAATCAGGTTTATTACAACGATATTTACAATCTTAAAAGTGATATCGAAGGTCTAGCAAATGTTAGTGAAGAACAGA
AACAAGCTCTAAGGCATATT

Downstream 100 bases:

>100_bases
TTCTTACGATTAAAAATTGAATGTAAGCTATTCATTCACAGAGCGAATTACAAAATGAGCTATACATAACAGCTCACAGT
GATGCTTGCAATTCGCATTA

Product: putative conjugative transfer protein TraA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 582; Mature: 582

Protein sequence:

>582_residues
MLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRRQKVIGLAPTHKAVSELRSKGYTEVYTVKGFLYNRKKNFMQGSLIVVD
EAGMVGTKAYAELFRVVRNNYCQLILAGDEKQLASIERGGMFEMLSNNFGSHVLIDIRRQSENWSREAATKFAESNILSG
ITLMRQNKCVKFDNTLQDSISKLIYDWSLSKFKLHEKLVITVRNKDVDILNSSIRSLLKANGTLQGTEYRRSIAGRKESY
MAGDRIVFQKSDKDLQIQNSEFATLTSVNKNEFVAKTDAGKEVSFDPSKIQFKHGYASTIYKAQGASIKDVYVLHNGVSN
ISSSYVAMTRHIEKLQLYCNKKATVSIKSLINQLSRPNEKSASITLKTAHDLEKERTKTTVFSKVFSKVENWFKSIINDI
NDRSHVNEEYYHFTAKPEEEAKVEKVQLKDISTPLFMHIKEQRQYDYDVTILSAEGKTISSFQEAGIDSRMVYSSNVNNL
KYYQPFQGEKILIAANNDKQNKEYASTINEAAKALKSKGAITSIVVPSEGEDFNEMLKNKGATAVKELMIPEIMKLINTQ
NVKTESEQVVKTDIAQKIVIRR

Sequences:

>Translated_582_residues
MLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRRQKVIGLAPTHKAVSELRSKGYTEVYTVKGFLYNRKKNFMQGSLIVVD
EAGMVGTKAYAELFRVVRNNYCQLILAGDEKQLASIERGGMFEMLSNNFGSHVLIDIRRQSENWSREAATKFAESNILSG
ITLMRQNKCVKFDNTLQDSISKLIYDWSLSKFKLHEKLVITVRNKDVDILNSSIRSLLKANGTLQGTEYRRSIAGRKESY
MAGDRIVFQKSDKDLQIQNSEFATLTSVNKNEFVAKTDAGKEVSFDPSKIQFKHGYASTIYKAQGASIKDVYVLHNGVSN
ISSSYVAMTRHIEKLQLYCNKKATVSIKSLINQLSRPNEKSASITLKTAHDLEKERTKTTVFSKVFSKVENWFKSIINDI
NDRSHVNEEYYHFTAKPEEEAKVEKVQLKDISTPLFMHIKEQRQYDYDVTILSAEGKTISSFQEAGIDSRMVYSSNVNNL
KYYQPFQGEKILIAANNDKQNKEYASTINEAAKALKSKGAITSIVVPSEGEDFNEMLKNKGATAVKELMIPEIMKLINTQ
NVKTESEQVVKTDIAQKIVIRR
>Mature_582_residues
MLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRRQKVIGLAPTHKAVSELRSKGYTEVYTVKGFLYNRKKNFMQGSLIVVD
EAGMVGTKAYAELFRVVRNNYCQLILAGDEKQLASIERGGMFEMLSNNFGSHVLIDIRRQSENWSREAATKFAESNILSG
ITLMRQNKCVKFDNTLQDSISKLIYDWSLSKFKLHEKLVITVRNKDVDILNSSIRSLLKANGTLQGTEYRRSIAGRKESY
MAGDRIVFQKSDKDLQIQNSEFATLTSVNKNEFVAKTDAGKEVSFDPSKIQFKHGYASTIYKAQGASIKDVYVLHNGVSN
ISSSYVAMTRHIEKLQLYCNKKATVSIKSLINQLSRPNEKSASITLKTAHDLEKERTKTTVFSKVFSKVENWFKSIINDI
NDRSHVNEEYYHFTAKPEEEAKVEKVQLKDISTPLFMHIKEQRQYDYDVTILSAEGKTISSFQEAGIDSRMVYSSNVNNL
KYYQPFQGEKILIAANNDKQNKEYASTINEAAKALKSKGAITSIVVPSEGEDFNEMLKNKGATAVKELMIPEIMKLINTQ
NVKTESEQVVKTDIAQKIVIRR

Specific function: traI Has Been Identified As DNA Helicase I And It Also Has An Additional Activity Of Site-Specific Nicking At Orit. DNA Helicase I Is A Potent DNA-Dependent ATPase. [C]

COG id: COG0507

COG function: function code L; ATP-dependent exoDNAse (exonuclease V), alpha subunit - helicase superfamily I member

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mobA/mobL family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000606
- InterPro:   IPR005053
- InterPro:   IPR014136 [H]

Pfam domain/function: PF03389 MobA_MobL; PF01443 Viral_helicase1 [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 65748; Mature: 65748

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRRQKVIGLAPTHKAVSELRSKGYTEVYTV
CCCCCCCCEEECCCCCCCEEEEEEEHHHHCCCCCEEEEECCCHHHHHHHHHCCCCEEEEE
KGFLYNRKKNFMQGSLIVVDEAGMVGTKAYAELFRVVRNNYCQLILAGDEKQLASIERGG
EHEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCC
MFEMLSNNFGSHVLIDIRRQSENWSREAATKFAESNILSGITLMRQNKCVKFDNTLQDSI
HHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHH
SKLIYDWSLSKFKLHEKLVITVRNKDVDILNSSIRSLLKANGTLQGTEYRRSIAGRKESY
HHHHHHCCCCCEEECEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHH
MAGDRIVFQKSDKDLQIQNSEFATLTSVNKNEFVAKTDAGKEVSFDPSKIQFKHGYASTI
CCCCEEEEEECCCCEEEECCCEEEEEECCCCCEEEECCCCCCCCCCCCCEEEECCCHHHH
YKAQGASIKDVYVLHNGVSNISSSYVAMTRHIEKLQLYCNKKATVSIKSLINQLSRPNEK
EECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCC
SASITLKTAHDLEKERTKTTVFSKVFSKVENWFKSIINDINDRSHVNEEYYHFTAKPEEE
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHEEEECCCCHH
AKVEKVQLKDISTPLFMHIKEQRQYDYDVTILSAEGKTISSFQEAGIDSRMVYSSNVNNL
HHHHHEEHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHCCCCCEEEEECCCCCC
KYYQPFQGEKILIAANNDKQNKEYASTINEAAKALKSKGAITSIVVPSEGEDFNEMLKNK
EEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCC
GATAVKELMIPEIMKLINTQNVKTESEQVVKTDIAQKIVIRR
CHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLSTSGVRVLRGRAGTGKSYVLIKAHKLATNRRQKVIGLAPTHKAVSELRSKGYTEVYTV
CCCCCCCCEEECCCCCCCEEEEEEEHHHHCCCCCEEEEECCCHHHHHHHHHCCCCEEEEE
KGFLYNRKKNFMQGSLIVVDEAGMVGTKAYAELFRVVRNNYCQLILAGDEKQLASIERGG
EHEEECCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHCCC
MFEMLSNNFGSHVLIDIRRQSENWSREAATKFAESNILSGITLMRQNKCVKFDNTLQDSI
HHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHH
SKLIYDWSLSKFKLHEKLVITVRNKDVDILNSSIRSLLKANGTLQGTEYRRSIAGRKESY
HHHHHHCCCCCEEECEEEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCHHH
MAGDRIVFQKSDKDLQIQNSEFATLTSVNKNEFVAKTDAGKEVSFDPSKIQFKHGYASTI
CCCCEEEEEECCCCEEEECCCEEEEEECCCCCEEEECCCCCCCCCCCCCEEEECCCHHHH
YKAQGASIKDVYVLHNGVSNISSSYVAMTRHIEKLQLYCNKKATVSIKSLINQLSRPNEK
EECCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCC
SASITLKTAHDLEKERTKTTVFSKVFSKVENWFKSIINDINDRSHVNEEYYHFTAKPEEE
CCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHEEEECCCCHH
AKVEKVQLKDISTPLFMHIKEQRQYDYDVTILSAEGKTISSFQEAGIDSRMVYSSNVNNL
HHHHHEEHHHCCCHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHCCCCCEEEEECCCCCC
KYYQPFQGEKILIAANNDKQNKEYASTINEAAKALKSKGAITSIVVPSEGEDFNEMLKNK
EEECCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHCC
GATAVKELMIPEIMKLINTQNVKTESEQVVKTDIAQKIVIRR
CHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on acid anhydrides; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8763953; 11743193; 11743194 [H]