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The map label for this gene is nuoI

Identifier: 189184497

GI number: 189184497

Start: 1622419

End: 1622904

Strand: Reverse

Name: nuoI

Synonym: OTT_1590

Alternate gene names: 189184497

Gene position: 1622904-1622419 (Counterclockwise)

Preceding gene: 189184498

Following gene: 189184496

Centisome position: 80.78

GC content: 32.72

Gene sequence:

>486_bases
ATGAAGATAACTAATTTTACTAAATCTTTTCTTTTGTATGAAATTATTGTTGGAATGTTTTTAACTTTGAAATATTTTTT
TAAATCTAAAGTTACGATTCGTTATCCTAATGAAGTTAGCAAATTGAGCCCAAGATTTAAAGGAGAGCATGCTTTAAGAC
GCTATCCAGATGGCGAAGAGAGATGTATAGCATGTAAATTATGCGAAGCTATTTGTCCAGCTCAAGCTATTACTATTGAA
GCAAAAGAGCAGCCGAATGGTAGTAGGCGCACTACTAAATATGATATTGATATGACTAAGTGTATATATTGCGGATTATG
TCAAGAGGCATGCCCAGTAGATGCTATTGTTGAAGGGCCAAATTTGGAATTTGCTACAGAAACTCATCAGGAATTATTGT
ATAACAAAGAGAAACTGTTAAGAAATGGTGATATGTGGGAACATGTAATAGCTAAAAATTTAAAAGTGGATAGTATTTAC
AGGTAG

Upstream 100 bases:

>100_bases
TAATAGTAGTATCAGCTATTTTAGTATATAGTAATAACTTACCATAGCACTAGAAACTGCGCTAGACAAATAATTGGATT
TATAATGTAGAGAATCGAAA

Downstream 100 bases:

>100_bases
TTAATGTTATTTTTTTATTTATTTTCCGTTTTAGCACTACTTGGGAGTATTGGAGTTATAACAAGCAAAAATCCAGTATA
TGCAGTATTGTGGTTAATAT

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MKITNFTKSFLLYEIIVGMFLTLKYFFKSKVTIRYPNEVSKLSPRFKGEHALRRYPDGEERCIACKLCEAICPAQAITIE
AKEQPNGSRRTTKYDIDMTKCIYCGLCQEACPVDAIVEGPNLEFATETHQELLYNKEKLLRNGDMWEHVIAKNLKVDSIY
R

Sequences:

>Translated_161_residues
MKITNFTKSFLLYEIIVGMFLTLKYFFKSKVTIRYPNEVSKLSPRFKGEHALRRYPDGEERCIACKLCEAICPAQAITIE
AKEQPNGSRRTTKYDIDMTKCIYCGLCQEACPVDAIVEGPNLEFATETHQELLYNKEKLLRNGDMWEHVIAKNLKVDSIY
R
>Mature_161_residues
MKITNFTKSFLLYEIIVGMFLTLKYFFKSKVTIRYPNEVSKLSPRFKGEHALRRYPDGEERCIACKLCEAICPAQAITIE
AKEQPNGSRRTTKYDIDMTKCIYCGLCQEACPVDAIVEGPNLEFATETHQELLYNKEKLLRNGDMWEHVIAKNLKVDSIY
R

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=155, Percent_Identity=70.9677419354839, Blast_Score=241, Evalue=2e-64,
Organism=Escherichia coli, GI1788617, Length=139, Percent_Identity=38.8489208633094, Blast_Score=107, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17555194, Length=148, Percent_Identity=71.6216216216216, Blast_Score=230, Evalue=3e-61,
Organism=Drosophila melanogaster, GI17864306, Length=160, Percent_Identity=71.25, Blast_Score=242, Evalue=8e-65,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI_ORITB (A5CFN6)

Other databases:

- EMBL:   AM494475
- RefSeq:   YP_001249253.1
- ProteinModelPortal:   A5CFN6
- SMR:   A5CFN6
- STRING:   A5CFN6
- GeneID:   5219203
- GenomeReviews:   AM494475_GR
- KEGG:   ots:OTBS_2161
- eggNOG:   COG1143
- HOGENOM:   HBG724359
- OMA:   NYPYEKG
- ProtClustDB:   PRK05888
- BioCyc:   OTSU357244:OTBS_2161-MONOMER
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 18641; Mature: 18641

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKITNFTKSFLLYEIIVGMFLTLKYFFKSKVTIRYPNEVSKLSPRFKGEHALRRYPDGEE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHCCCCCCCCHHHHCCCCCCH
RCIACKLCEAICPAQAITIEAKEQPNGSRRTTKYDIDMTKCIYCGLCQEACPVDAIVEGP
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEECCC
NLEFATETHQELLYNKEKLLRNGDMWEHVIAKNLKVDSIYR
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKITNFTKSFLLYEIIVGMFLTLKYFFKSKVTIRYPNEVSKLSPRFKGEHALRRYPDGEE
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCHHHHCCCCCCCCHHHHCCCCCCH
RCIACKLCEAICPAQAITIEAKEQPNGSRRTTKYDIDMTKCIYCGLCQEACPVDAIVEGP
HHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEECCC
NLEFATETHQELLYNKEKLLRNGDMWEHVIAKNLKVDSIYR
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA