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The map label for this gene is mesJ [H]

Identifier: 189184491

GI number: 189184491

Start: 1614954

End: 1616279

Strand: Reverse

Name: mesJ [H]

Synonym: OTT_1584

Alternate gene names: 189184491

Gene position: 1616279-1614954 (Counterclockwise)

Preceding gene: 189184492

Following gene: 189184490

Centisome position: 80.45

GC content: 29.49

Gene sequence:

>1326_bases
ATGAATATTGAGGTAGATTTTTGTCATAATATGGAGTGTTTTGGAGCTTTTGAAAAAAATCCAAAGCTCGCTATAGCTGT
CTCTGGAGGAAGTGACTCACTGGCTTTAATGTTACTAGTGAAACACTGGAACGAGAAGGTAAAAGGAGAAATAACTATTT
TAACAATAGATCATCACTTGCGTTCTGAATCAACTAGCGAAGCAGATTATGTTAGTAGTATATGTCAGAATATAAAACTT
CAGCATGTTACTCTACACTGGATACATAAAGGAATTACAGGAAATATTCAAGCTCAGGCTCGTAAAGCAAGATATCAGTT
GTTAACTAACTATTGTCAAGAGCATGACATTTTACATTTGATTACAGGACATCATGCAGATGATATAGTTGAAAATTTTT
TCATAAGATTACTTCGTGGAGCAGGATTAGCTGGATTGAGTTCTCATAATCTTTTCTTTGTTAATAATGTTCGAATTATT
CGTCCACTATTTAATATTACTAAACAGGATTTAAAAAAATATTTAGAACAGCAGAATATTAAATGGATAAATGATCCATC
TAATAGTTCAAATAAGTATCTTCGTACTCAAGTTCGTGATTTATTAAAATCTATGCTTATTTCTTTTCAGCATAATTTTA
CAGTAGAATTACTAAAAAAAAGAATAATGCTTAGCCAAATGCATTTAACACAAGCTTTAGAAAGCGTAAATAATGAAATA
ATTCACTATGTAGTCCATGTAGTTAAAATTTATTCAGCTGGTTTTGCTGTAATCGATAGGAAGCTTTTTAAGAAAGCTTC
GCCTGAGGCTCGATATGCTATATTATCATACTTACTGATGATTGTTGGTGCTAATACAAAACCTCCAAGATTTTCTTCTT
TACAACGCATTAGTTTGCATGATATACAGGAATATAATACTTATAAAACTTTACATGGTTGCATTGTTGAATATTCTATA
GAATATATTATAATATATAGAGAGTTTGGGCGTTGCTATCCTATATCACAGGTTGTAAGTAATTCAGTAGTTTGGGACTA
TAGATTTAAAGTAGTTGATAATAGAAAAAATAATCATATGAATTTAACTATTGATTATTTAAAAAAAAGCGACTATCACT
TAATTAAATCATATGTTGAAAGTAATCAGAGAAATGCTTACTTTAACTACAGTAAGAAAATATTATTTACTTTTCCTGTG
ATTAAACATCTTGAAAAAGTCGTAGCGATTCCACATATAAAATACTATAGTGATAAAACAATTCAAGAAAGTGTAAGTTT
TGTTTTTGAACCGAAGTTAATATCGCGTTGGTTTCATTATTGTTAA

Upstream 100 bases:

>100_bases
ATTTTTAATTTTATGAATGAGTTAATTTTAGCAAATTAACTTTAAGTTAAAGTTGATGATTAAAATCGTTAATTGAATTA
AAGTAAAAACAACGTAATCA

Downstream 100 bases:

>100_bases
AAAATTTAGGGTTAATATGAATAATCAAAACAGGAATGTATTATTTTGGGCAACAGTATTTATTGTACTTGTTGTAGCAT
TTAACTTGCTGCAAGGAGAG

Product: MesJ protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MNIEVDFCHNMECFGAFEKNPKLAIAVSGGSDSLALMLLVKHWNEKVKGEITILTIDHHLRSESTSEADYVSSICQNIKL
QHVTLHWIHKGITGNIQAQARKARYQLLTNYCQEHDILHLITGHHADDIVENFFIRLLRGAGLAGLSSHNLFFVNNVRII
RPLFNITKQDLKKYLEQQNIKWINDPSNSSNKYLRTQVRDLLKSMLISFQHNFTVELLKKRIMLSQMHLTQALESVNNEI
IHYVVHVVKIYSAGFAVIDRKLFKKASPEARYAILSYLLMIVGANTKPPRFSSLQRISLHDIQEYNTYKTLHGCIVEYSI
EYIIIYREFGRCYPISQVVSNSVVWDYRFKVVDNRKNNHMNLTIDYLKKSDYHLIKSYVESNQRNAYFNYSKKILFTFPV
IKHLEKVVAIPHIKYYSDKTIQESVSFVFEPKLISRWFHYC

Sequences:

>Translated_441_residues
MNIEVDFCHNMECFGAFEKNPKLAIAVSGGSDSLALMLLVKHWNEKVKGEITILTIDHHLRSESTSEADYVSSICQNIKL
QHVTLHWIHKGITGNIQAQARKARYQLLTNYCQEHDILHLITGHHADDIVENFFIRLLRGAGLAGLSSHNLFFVNNVRII
RPLFNITKQDLKKYLEQQNIKWINDPSNSSNKYLRTQVRDLLKSMLISFQHNFTVELLKKRIMLSQMHLTQALESVNNEI
IHYVVHVVKIYSAGFAVIDRKLFKKASPEARYAILSYLLMIVGANTKPPRFSSLQRISLHDIQEYNTYKTLHGCIVEYSI
EYIIIYREFGRCYPISQVVSNSVVWDYRFKVVDNRKNNHMNLTIDYLKKSDYHLIKSYVESNQRNAYFNYSKKILFTFPV
IKHLEKVVAIPHIKYYSDKTIQESVSFVFEPKLISRWFHYC
>Mature_441_residues
MNIEVDFCHNMECFGAFEKNPKLAIAVSGGSDSLALMLLVKHWNEKVKGEITILTIDHHLRSESTSEADYVSSICQNIKL
QHVTLHWIHKGITGNIQAQARKARYQLLTNYCQEHDILHLITGHHADDIVENFFIRLLRGAGLAGLSSHNLFFVNNVRII
RPLFNITKQDLKKYLEQQNIKWINDPSNSSNKYLRTQVRDLLKSMLISFQHNFTVELLKKRIMLSQMHLTQALESVNNEI
IHYVVHVVKIYSAGFAVIDRKLFKKASPEARYAILSYLLMIVGANTKPPRFSSLQRISLHDIQEYNTYKTLHGCIVEYSI
EYIIIYREFGRCYPISQVVSNSVVWDYRFKVVDNRKNNHMNLTIDYLKKSDYHLIKSYVESNQRNAYFNYSKKILFTFPV
IKHLEKVVAIPHIKYYSDKTIQESVSFVFEPKLISRWFHYC

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=190, Percent_Identity=30.5263157894737, Blast_Score=88, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 51539; Mature: 51539

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIEVDFCHNMECFGAFEKNPKLAIAVSGGSDSLALMLLVKHWNEKVKGEITILTIDHHL
CCEEEEECCCCHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCHH
RSESTSEADYVSSICQNIKLQHVTLHWIHKGITGNIQAQARKARYQLLTNYCQEHDILHL
CCCCCCHHHHHHHHHHCCEEEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEE
ITGHHADDIVENFFIRLLRGAGLAGLSSHNLFFVNNVRIIRPLFNITKQDLKKYLEQQNI
ECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCC
KWINDPSNSSNKYLRTQVRDLLKSMLISFQHNFTVELLKKRIMLSQMHLTQALESVNNEI
EEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHYVVHVVKIYSAGFAVIDRKLFKKASPEARYAILSYLLMIVGANTKPPRFSSLQRISLH
HHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
DIQEYNTYKTLHGCIVEYSIEYIIIYREFGRCYPISQVVSNSVVWDYRFKVVDNRKNNHM
HHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCHHHHHCCCEEEEEEEEEEECCCCCCE
NLTIDYLKKSDYHLIKSYVESNQRNAYFNYSKKILFTFPVIKHLEKVVAIPHIKYYSDKT
EEEEEECCCCCHHHHHHHHHCCCCCEEEECCCEEEEEHHHHHHHHHHHHCCCEEECCCCH
IQESVSFVFEPKLISRWFHYC
HHHHHHHHCCHHHHHHHHHCC
>Mature Secondary Structure
MNIEVDFCHNMECFGAFEKNPKLAIAVSGGSDSLALMLLVKHWNEKVKGEITILTIDHHL
CCEEEEECCCCHHHCCCCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEEECCHH
RSESTSEADYVSSICQNIKLQHVTLHWIHKGITGNIQAQARKARYQLLTNYCQEHDILHL
CCCCCCHHHHHHHHHHCCEEEEEEEEEHHCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEE
ITGHHADDIVENFFIRLLRGAGLAGLSSHNLFFVNNVRIIRPLFNITKQDLKKYLEQQNI
ECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCC
KWINDPSNSSNKYLRTQVRDLLKSMLISFQHNFTVELLKKRIMLSQMHLTQALESVNNEI
EEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHYVVHVVKIYSAGFAVIDRKLFKKASPEARYAILSYLLMIVGANTKPPRFSSLQRISLH
HHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
DIQEYNTYKTLHGCIVEYSIEYIIIYREFGRCYPISQVVSNSVVWDYRFKVVDNRKNNHM
HHHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCCHHHHHCCCEEEEEEEEEEECCCCCCE
NLTIDYLKKSDYHLIKSYVESNQRNAYFNYSKKILFTFPVIKHLEKVVAIPHIKYYSDKT
EEEEEECCCCCHHHHHHHHHCCCCCEEEECCCEEEEEHHHHHHHHHHHHCCCEEECCCCH
IQESVSFVFEPKLISRWFHYC
HHHHHHHHCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA