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The map label for this gene is prfB [H]

Identifier: 189183642

GI number: 189183642

Start: 760487

End: 761350

Strand: Direct

Name: prfB [H]

Synonym: OTT_0735

Alternate gene names: 189183642

Gene position: 760487-761350 (Clockwise)

Preceding gene: 189183639

Following gene: 189183645

Centisome position: 37.85

GC content: 33.1

Gene sequence:

>864_bases
TTGAGCAACATTGCTATCAAAGAAAACGATATTGAGTTTATTCATAATATAGAACAAGAATGGAAAAAACTACAGGAAGA
AGCTAAAAAGCTACAGGTCGAGTGCTTATTTACTAGTGATGTTGATGTTAATGATTGTTTTCTTGATATTAATGCTGGAG
CTGGAGGTACTGAAAGTAACGACTGGGCCTCAATGATGTTAAGAATGTATCTAAGATTTGCTGAAAGCATGAATTTTAAA
ACAGAGGTTGTTTATTTATTAGATGGTGAGGAAGCAGGTGTTAAATCAGCATCAGTTAAAATCTGTGGACATAATGCTTA
CGGATGGTTTAAAACAGAATCAGGAGTACATCGTCTTGTTAGAAATTCTCCTTTTAATGCTCTTGGTAAAAGAATGACCA
GTTTTGCTAGTGCGTGGGTATACCCGGTGATTAATGATAATATTCAAGTAGTTATTGAGGATAAAGATATTAGAATCGAT
ACTTATAGAGCTTCTGGAGCAGGAGGACAACATGTTAATACAACTGATTCAGCGATACGTATCACTCACTTTCCTACTAA
AATTGTTGTACAATGCCAAAATGATCGATCTCAACACAAAAATAAAGCTGAAGCTATGAGAATGTTAAAAGCAAAAATTT
ATGAGCTAGAACAAAAAAAACAAAATGAAATCATTAATAACCATAATGCTGCTAAAACAGATAATAGTTGGGGATATCAC
ATCAGATCTTATGTTCTGCAGCCATATCAACTAGTTAAAGATCTACGTACTAACGTTGAAACTTCTGATACAAAACGTGT
ATTAGATGGTGATTTGGAGAAATTTATTTATGCTAGCTTAATACACCAGAATATTACATTCTAA

Upstream 100 bases:

>100_bases
TTATCAACATGCATCTTTAGTCTTAAAGGAAAAAAAAACTATAGAGAATAAGTTAGCTACATATAATAAAATTGCTTCAG
CATTTGATGATTATGTTGAA

Downstream 100 bases:

>100_bases
TTATAGAAGAACTAACAAAAACAGGAATTCTCTTGAGAGAATTAGCATGTGCTATGAAGCATAGATAAACTAGGTATTCA
TCATTGTGTAAATTACAGTA

Product: peptide chain release factor RF-2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSNIAIKENDIEFIHNIEQEWKKLQEEAKKLQVECLFTSDVDVNDCFLDINAGAGGTESNDWASMMLRMYLRFAESMNFK
TEVVYLLDGEEAGVKSASVKICGHNAYGWFKTESGVHRLVRNSPFNALGKRMTSFASAWVYPVINDNIQVVIEDKDIRID
TYRASGAGGQHVNTTDSAIRITHFPTKIVVQCQNDRSQHKNKAEAMRMLKAKIYELEQKKQNEIINNHNAAKTDNSWGYH
IRSYVLQPYQLVKDLRTNVETSDTKRVLDGDLEKFIYASLIHQNITF

Sequences:

>Translated_287_residues
MSNIAIKENDIEFIHNIEQEWKKLQEEAKKLQVECLFTSDVDVNDCFLDINAGAGGTESNDWASMMLRMYLRFAESMNFK
TEVVYLLDGEEAGVKSASVKICGHNAYGWFKTESGVHRLVRNSPFNALGKRMTSFASAWVYPVINDNIQVVIEDKDIRID
TYRASGAGGQHVNTTDSAIRITHFPTKIVVQCQNDRSQHKNKAEAMRMLKAKIYELEQKKQNEIINNHNAAKTDNSWGYH
IRSYVLQPYQLVKDLRTNVETSDTKRVLDGDLEKFIYASLIHQNITF
>Mature_286_residues
SNIAIKENDIEFIHNIEQEWKKLQEEAKKLQVECLFTSDVDVNDCFLDINAGAGGTESNDWASMMLRMYLRFAESMNFKT
EVVYLLDGEEAGVKSASVKICGHNAYGWFKTESGVHRLVRNSPFNALGKRMTSFASAWVYPVINDNIQVVIEDKDIRIDT
YRASGAGGQHVNTTDSAIRITHFPTKIVVQCQNDRSQHKNKAEAMRMLKAKIYELEQKKQNEIINNHNAAKTDNSWGYHI
RSYVLQPYQLVKDLRTNVETSDTKRVLDGDLEKFIYASLIHQNITF

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=237, Percent_Identity=40.5063291139241, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI34577120, Length=191, Percent_Identity=39.2670157068063, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI166795305, Length=149, Percent_Identity=42.2818791946309, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI2367172, Length=277, Percent_Identity=55.2346570397112, Blast_Score=322, Evalue=1e-89,
Organism=Escherichia coli, GI1787462, Length=241, Percent_Identity=40.6639004149378, Blast_Score=177, Evalue=8e-46,
Organism=Caenorhabditis elegans, GI17542784, Length=208, Percent_Identity=33.6538461538462, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6321295, Length=208, Percent_Identity=34.6153846153846, Blast_Score=121, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19921226, Length=224, Percent_Identity=37.0535714285714, Blast_Score=144, Evalue=9e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 32850; Mature: 32718

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIAIKENDIEFIHNIEQEWKKLQEEAKKLQVECLFTSDVDVNDCFLDINAGAGGTESN
CCCCEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCEEEEEEECCCCCCCCC
DWASMMLRMYLRFAESMNFKTEVVYLLDGEEAGVKSASVKICGHNAYGWFKTESGVHRLV
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCEEECCHHHHHHH
RNSPFNALGKRMTSFASAWVYPVINDNIQVVIEDKDIRIDTYRASGAGGQHVNTTDSAIR
HCCCHHHHHHHHHHHHHHEEEEEECCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCEEE
ITHFPTKIVVQCQNDRSQHKNKAEAMRMLKAKIYELEQKKQNEIINNHNAAKTDNSWGYH
EEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
IRSYVLQPYQLVKDLRTNVETSDTKRVLDGDLEKFIYASLIHQNITF
HHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SNIAIKENDIEFIHNIEQEWKKLQEEAKKLQVECLFTSDVDVNDCFLDINAGAGGTESN
CCCEECCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCEEEEEEECCCCCCCCC
DWASMMLRMYLRFAESMNFKTEVVYLLDGEEAGVKSASVKICGHNAYGWFKTESGVHRLV
HHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCCEEEEEECCCCCEEECCHHHHHHH
RNSPFNALGKRMTSFASAWVYPVINDNIQVVIEDKDIRIDTYRASGAGGQHVNTTDSAIR
HCCCHHHHHHHHHHHHHHEEEEEECCCEEEEEECCCEEEEEEECCCCCCCCCCCCCCEEE
ITHFPTKIVVQCQNDRSQHKNKAEAMRMLKAKIYELEQKKQNEIINNHNAAKTDNSWGYH
EEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
IRSYVLQPYQLVKDLRTNVETSDTKRVLDGDLEKFIYASLIHQNITF
HHHHHHCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9823893 [H]