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The map label for this gene is mjaIIIM [H]

Identifier: 189183348

GI number: 189183348

Start: 462136

End: 462768

Strand: Direct

Name: mjaIIIM [H]

Synonym: OTT_0441

Alternate gene names: 189183348

Gene position: 462136-462768 (Clockwise)

Preceding gene: 189183347

Following gene: 189183350

Centisome position: 23.0

GC content: 29.07

Gene sequence:

>633_bases
TTGTCTGATATTAATCTCGACTTAATTACTAGTTATCACGCTGTAAAAAAGAATCCAAATGAGGTAAATAGATTACTAAA
TCTATATCACAAAAACCATTCTGAAAACTACTACTATAAAATAAGAGACAATTATTATAGTAATGATCCTAATGATATCA
CTGCAAAATTTATATATCTTAATAAATATTCCTTTAGGGGAATTTATAGGCTAAACAGAGATGGGACATCTGCTCAAACA
TTTTCTGATAAGCGATATCTTAAGCTTCATATTTGCTCTAGAATAAATAAATGTAGTAACCTTCTTGCTGGTGTATCAAT
TTACGCTACAGATTTTTCATTTATAGAGCCTCAGCAAAATGACTTTGTTTATTTTGATCCTCCTTACCATAAATCTGGAG
AAAGGTTCTACACTAGACTTCCATTTGATGAAAAAGATCAAATTAGATTACGGGATTTTGTCCAAGAATTAACAAATAAA
GGTGTTAAAATTATGCTTTCAAATAATAACACTACCTTCATTAGAGACTTATATAAAGACTTCTTCATCACTCATATTGG
AGTTACATACTCAATCAATGAACAACGCAATCCTGTTAATGAACTTATCATTACTAACTATAAACGTAACTAA

Upstream 100 bases:

>100_bases
AGAGCATCTTCCTTCAGGGCCATACTATAATTACTATGAACCATTCCTTGGAGGTGGGGCTTTATTTTTTCAAGTTAGGC
ATCTGTTTAAACAATGTTTT

Downstream 100 bases:

>100_bases
GTTAAACGGGAGCAGTTTTTTACAAAAAGTTAGTGGATAATAATTATTATGAATAATTTTGCAACTTACATAAAGTATAA
AATTTACTATTGCTAATACT

Product: N6 adenine-specific DNA-methyltransferase

Products: NA

Alternate protein names: M.MjaIII; Adenine-specific methyltransferase MjaIII [H]

Number of amino acids: Translated: 210; Mature: 209

Protein sequence:

>210_residues
MSDINLDLITSYHAVKKNPNEVNRLLNLYHKNHSENYYYKIRDNYYSNDPNDITAKFIYLNKYSFRGIYRLNRDGTSAQT
FSDKRYLKLHICSRINKCSNLLAGVSIYATDFSFIEPQQNDFVYFDPPYHKSGERFYTRLPFDEKDQIRLRDFVQELTNK
GVKIMLSNNNTTFIRDLYKDFFITHIGVTYSINEQRNPVNELIITNYKRN

Sequences:

>Translated_210_residues
MSDINLDLITSYHAVKKNPNEVNRLLNLYHKNHSENYYYKIRDNYYSNDPNDITAKFIYLNKYSFRGIYRLNRDGTSAQT
FSDKRYLKLHICSRINKCSNLLAGVSIYATDFSFIEPQQNDFVYFDPPYHKSGERFYTRLPFDEKDQIRLRDFVQELTNK
GVKIMLSNNNTTFIRDLYKDFFITHIGVTYSINEQRNPVNELIITNYKRN
>Mature_209_residues
SDINLDLITSYHAVKKNPNEVNRLLNLYHKNHSENYYYKIRDNYYSNDPNDITAKFIYLNKYSFRGIYRLNRDGTSAQTF
SDKRYLKLHICSRINKCSNLLAGVSIYATDFSFIEPQQNDFVYFDPPYHKSGERFYTRLPFDEKDQIRLRDFVQELTNKG
VKIMLSNNNTTFIRDLYKDFFITHIGVTYSINEQRNPVNELIITNYKRN

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MjaIII endonuclease (Potential) [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 25100; Mature: 24968

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDINLDLITSYHAVKKNPNEVNRLLNLYHKNHSENYYYKIRDNYYSNDPNDITAKFIYL
CCCCCEEEEEHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEEEE
NKYSFRGIYRLNRDGTSAQTFSDKRYLKLHICSRINKCSNLLAGVSIYATDFSFIEPQQN
ECCCCCEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCEEEEEECCEEECCCCC
DFVYFDPPYHKSGERFYTRLPFDEKDQIRLRDFVQELTNKGVKIMLSNNNTTFIRDLYKD
CEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
FFITHIGVTYSINEQRNPVNELIITNYKRN
HHHHHEEEEEEECCCCCCCHHEEEECCCCC
>Mature Secondary Structure 
SDINLDLITSYHAVKKNPNEVNRLLNLYHKNHSENYYYKIRDNYYSNDPNDITAKFIYL
CCCCEEEEEHHHHHCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCEEEEEEEE
NKYSFRGIYRLNRDGTSAQTFSDKRYLKLHICSRINKCSNLLAGVSIYATDFSFIEPQQN
ECCCCCEEEEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHCEEEEEECCEEECCCCC
DFVYFDPPYHKSGERFYTRLPFDEKDQIRLRDFVQELTNKGVKIMLSNNNTTFIRDLYKD
CEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHH
FFITHIGVTYSINEQRNPVNELIITNYKRN
HHHHHEEEEEEECCCCCCCHHEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]