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The map label for this gene is lysS2 [H]

Identifier: 189026263

GI number: 189026263

Start: 1137610

End: 1138665

Strand: Reverse

Name: lysS2 [H]

Synonym: TPASS_1040

Alternate gene names: 189026263

Gene position: 1138665-1137610 (Counterclockwise)

Preceding gene: 189026264

Following gene: 189026261

Centisome position: 99.93

GC content: 52.18

Gene sequence:

>1056_bases
ATGGATACCGAATCTGTCCTCTTTCGCGCGCAGTGCTTGCGTGCAGTGCGTGATTTTTTCCTTGAACACCACTACATAGA
GCTCGATACGCCTGCACTCGCCCGTGCGCTCGTTCCAGAACGGTGTCTTGAGGTGTTTCAAACCGAGTACTTTACGTCAG
TGCATGCTAAAGATACACAGAAGTTATATCTCGTTCCCTCTCCTGAGGTTTTTCTGAAACCGCTCATCGCGCAACTGCAA
CGTTCGGCTTTTCAGATCTCAAAGTGCTATCGCAATGGAGAGTCCATGGGCGCCTTGCATAGGCCGGAATTTACTATGGT
CGAATACTACACGGTGTACGCTGACTACAAGACGTCGCTCGATGTAAGCAGCAAACTCTTTCGCTTTGTGGTTGAACAAG
TACAGAGTCATCCGCTCGCGGACCCATATTCGTGTGCTTGTTTTTGTGCTCCCTTCGAGTACGTGACGGTCGAGGAAGCT
TTTCTCCGCTATGCAGGCTTTTCCCTTTCGCACGCGAGTAGTGTACAGACGCTTGCGCAGGAAGTATTGCGCTCCGGAAT
AGACCTGGGAGCACGTGCGGGGGTCGATTATACCCAGTGGTCATGGGACGATTTGTACGAACTGTTGCTCGTGCATATTG
TTGAACCAAAGTTGAGGTCAATAAAGGATCGTTGCGTCGTGCTGTATGACTATCCTATACAGATATCCTGCCTGGCGCAG
GAACACACTGGACGCTCAGGGATACAATCTACGTCACCTAACAAGGGTGACGCACCTCACTGGGTGGTAAAGGAACGGTG
GGAACTGTACGTCCGCGGTGTGGAACTCATAAACTGTTACACAGAGCAGCGGGATGCGAAGCATGTTACCCGGTACTGCA
GGGAAGAACAAACCGCAAAACAGGGATCTGCGCGAGTTGTGCATCCTGTTCCAGAGGGCTTTGCGCACGCGTGCGCACGC
ATGCCCCCTTGCTCTGGAGCAGCACTCGGATTTGATCGCCTGGTTGCGCTGCTAGCCGGTCGGCACTCATTAGATGCGTT
TGTGTATGATCAGTGA

Upstream 100 bases:

>100_bases
AGGATACAAATCGAGACTACTGGCTCGACGCTTCTCAAGCACTAGAATATGGTCTCATTTCGAACCTGATTGAAAAAAGG
GCGGACCTTCCTAAGAAATA

Downstream 100 bases:

>100_bases
CACTCCTCCTGCCTTGGAGAAGTTAATTGGAAGTTTCCTGGTTGTATTCGATGAGCGTTCTCACGGGAAGATCCCCAATC
AGCTCATGGTACCGTAGGAA

Product: lysyl-tRNA synthetase

Products: AMP; diphosphate; L-lysyl-tRNA(Lys)

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MDTESVLFRAQCLRAVRDFFLEHHYIELDTPALARALVPERCLEVFQTEYFTSVHAKDTQKLYLVPSPEVFLKPLIAQLQ
RSAFQISKCYRNGESMGALHRPEFTMVEYYTVYADYKTSLDVSSKLFRFVVEQVQSHPLADPYSCACFCAPFEYVTVEEA
FLRYAGFSLSHASSVQTLAQEVLRSGIDLGARAGVDYTQWSWDDLYELLLVHIVEPKLRSIKDRCVVLYDYPIQISCLAQ
EHTGRSGIQSTSPNKGDAPHWVVKERWELYVRGVELINCYTEQRDAKHVTRYCREEQTAKQGSARVVHPVPEGFAHACAR
MPPCSGAALGFDRLVALLAGRHSLDAFVYDQ

Sequences:

>Translated_351_residues
MDTESVLFRAQCLRAVRDFFLEHHYIELDTPALARALVPERCLEVFQTEYFTSVHAKDTQKLYLVPSPEVFLKPLIAQLQ
RSAFQISKCYRNGESMGALHRPEFTMVEYYTVYADYKTSLDVSSKLFRFVVEQVQSHPLADPYSCACFCAPFEYVTVEEA
FLRYAGFSLSHASSVQTLAQEVLRSGIDLGARAGVDYTQWSWDDLYELLLVHIVEPKLRSIKDRCVVLYDYPIQISCLAQ
EHTGRSGIQSTSPNKGDAPHWVVKERWELYVRGVELINCYTEQRDAKHVTRYCREEQTAKQGSARVVHPVPEGFAHACAR
MPPCSGAALGFDRLVALLAGRHSLDAFVYDQ
>Mature_351_residues
MDTESVLFRAQCLRAVRDFFLEHHYIELDTPALARALVPERCLEVFQTEYFTSVHAKDTQKLYLVPSPEVFLKPLIAQLQ
RSAFQISKCYRNGESMGALHRPEFTMVEYYTVYADYKTSLDVSSKLFRFVVEQVQSHPLADPYSCACFCAPFEYVTVEEA
FLRYAGFSLSHASSVQTLAQEVLRSGIDLGARAGVDYTQWSWDDLYELLLVHIVEPKLRSIKDRCVVLYDYPIQISCLAQ
EHTGRSGIQSTSPNKGDAPHWVVKERWELYVRGVELINCYTEQRDAKHVTRYCREEQTAKQGSARVVHPVPEGFAHACAR
MPPCSGAALGFDRLVALLAGRHSLDAFVYDQ

Specific function: Could Be A Lysyl-Trna Synthetase. Mutants In Poxa Have A Reduced Pyruvate Oxidase Activity And A Reduced Growth Rate. [C]

COG id: COG2269

COG function: function code J; Truncated, possibly inactive, lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=357, Percent_Identity=23.8095238095238, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI194272210, Length=357, Percent_Identity=23.249299719888, Blast_Score=93, Evalue=4e-19,
Organism=Escherichia coli, GI87082379, Length=343, Percent_Identity=30.6122448979592, Blast_Score=127, Evalue=8e-31,
Organism=Escherichia coli, GI1789256, Length=342, Percent_Identity=24.2690058479532, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1790571, Length=347, Percent_Identity=24.207492795389, Blast_Score=110, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI71994340, Length=353, Percent_Identity=23.7960339943343, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17535927, Length=353, Percent_Identity=23.7960339943343, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=353, Percent_Identity=23.7960339943343, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6324256, Length=361, Percent_Identity=23.5457063711911, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004525
- InterPro:   IPR018149 [H]

Pfam domain/function: PF00152 tRNA-synt_2 [H]

EC number: 6.1.1.6

Molecular weight: Translated: 39986; Mature: 39986

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTESVLFRAQCLRAVRDFFLEHHYIELDTPALARALVPERCLEVFQTEYFTSVHAKDTQ
CCHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KLYLVPSPEVFLKPLIAQLQRSAFQISKCYRNGESMGALHRPEFTMVEYYTVYADYKTSL
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCCC
DVSSKLFRFVVEQVQSHPLADPYSCACFCAPFEYVTVEEAFLRYAGFSLSHASSVQTLAQ
CHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EVLRSGIDLGARAGVDYTQWSWDDLYELLLVHIVEPKLRSIKDRCVVLYDYPIQISCLAQ
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHCCCEEEEEECCEEEEEEEC
EHTGRSGIQSTSPNKGDAPHWVVKERWELYVRGVELINCYTEQRDAKHVTRYCREEQTAK
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGSARVVHPVPEGFAHACARMPPCSGAALGFDRLVALLAGRHSLDAFVYDQ
CCCCEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECC
>Mature Secondary Structure
MDTESVLFRAQCLRAVRDFFLEHHYIELDTPALARALVPERCLEVFQTEYFTSVHAKDTQ
CCHHHHHHHHHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KLYLVPSPEVFLKPLIAQLQRSAFQISKCYRNGESMGALHRPEFTMVEYYTVYADYKTSL
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCCC
DVSSKLFRFVVEQVQSHPLADPYSCACFCAPFEYVTVEEAFLRYAGFSLSHASSVQTLAQ
CHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EVLRSGIDLGARAGVDYTQWSWDDLYELLLVHIVEPKLRSIKDRCVVLYDYPIQISCLAQ
HHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHCCCEEEEEECCEEEEEEEC
EHTGRSGIQSTSPNKGDAPHWVVKERWELYVRGVELINCYTEQRDAKHVTRYCREEQTAK
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGSARVVHPVPEGFAHACARMPPCSGAALGFDRLVALLAGRHSLDAFVYDQ
CCCCEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 2454 [C]

Specific activity: 1.79

Km value (mM): 0.0236 {L-Lys}} 0.0164 {L-Lys}} 0.0651 {ATP}} 0.0232 {ATP}} [C]

Substrates: ATP; L-lysine; tRNA(Lys)

Specific reaction: ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 6-Amino-n-hexanoic acid; Adenosine; ATP; Cadaverine; L-Lysineamide; L-Lysinehydroxamate; N-epsilon-Acetyl -L-lysine [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]