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The map label for this gene is sigA [H]

Identifier: 189026235

GI number: 189026235

Start: 1104485

End: 1105420

Strand: Reverse

Name: sigA [H]

Synonym: TPASS_1012

Alternate gene names: 189026235

Gene position: 1105420-1104485 (Counterclockwise)

Preceding gene: 189026237

Following gene: 189026234

Centisome position: 97.01

GC content: 50.11

Gene sequence:

>936_bases
TTGCTGAGTATATGGTACGTACCTTTCGAACAAAAGGAGTACTCTGTGAGACAAATGCAGTTCCGCCACGATGAGAATAC
TCTCACGGCATATTTGAATGACCTGAAACGGATACCGCTCATGAACGAAAAGGAAGAATATGCATGTGCATGTGCTGCCT
ATGCCGGGGATGCGCAAGCTAAAAACGCGCTCGTGCGTGCAAACGTACGCTTTGTTATCAGTGTTGCCAGGCATTACCGC
ACGTGTGGCTTACCCCTTGCCGATCTTATCAGTGAAGGCAATATTGGTCTTTTGACGGCTGCCGAGCGGTTCAATCCAGC
AAGGGGGTGTCGCTTTATTTCTTACGCTGCCTGTTGGATTCGCCAGTCTATCCTCAAGGCACTGAACGAAAAAGCACGCA
TCATCCGGTTGCCCGTGAGTCGTATTCGTGCTGCAAAGAGCACGTATGAGTACATACCTCCTGTTACCTCGGTGAATGTT
GGTATATCGAAAACACAGGGAGGCAACAGATATGGCAAAAGGCGTGCAACGATGTGCCGAGCTGTGCCAACAGTGGAAGA
AGTTCACATCTGGCACACGCCGCTATCTCTAGATGCTCCGCTTGGAGCCTCGGGAAGTCGTGGTGAGTCTGGGGATGTTG
ATGGACTGCGTATAGGTGACTGCGTGCCGGACGATCTTTACGCGCAGCCTGAGGAGCATATGCTTGCATGTGCATTGCAA
GCTGATATTGCAAAGATTTTACGACTTTTGCCTGCGCGTGATGCGCAGGTCATCCGCTATCGATTTGGACTTGGCGGGTA
TGAACGGCGTTCTCTGCAAGAGATTGGAGAAATTTTTCAGATAACAAAGGAGCGCGTTCGCCAAATAGAAAAAAAGGCTT
TGTTGCGTATCCGTAGCTGTGCCCGTCAACACAGACTGGATTCCTACATAGCGTAG

Upstream 100 bases:

>100_bases
TGTAGCGCGCCGTGGGAAAGGGAGGTATGCACAGAGGGTCCAGTGGCGTGATAGGGACACCGAACAGATCTCCTCTGGTT
TATCGTAAATGGCATGATAT

Downstream 100 bases:

>100_bases
ACATCAGAGGCGTATGAAATAGGAGCAGGACGCGTTGGGCATACAACTTATAGTGTTTTTAGGAAATCCTGGTGCAGAGT
ACGAAGAAACGCGGCACAAT

Product: RNA polymerase sigma-43 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MLSIWYVPFEQKEYSVRQMQFRHDENTLTAYLNDLKRIPLMNEKEEYACACAAYAGDAQAKNALVRANVRFVISVARHYR
TCGLPLADLISEGNIGLLTAAERFNPARGCRFISYAACWIRQSILKALNEKARIIRLPVSRIRAAKSTYEYIPPVTSVNV
GISKTQGGNRYGKRRATMCRAVPTVEEVHIWHTPLSLDAPLGASGSRGESGDVDGLRIGDCVPDDLYAQPEEHMLACALQ
ADIAKILRLLPARDAQVIRYRFGLGGYERRSLQEIGEIFQITKERVRQIEKKALLRIRSCARQHRLDSYIA

Sequences:

>Translated_311_residues
MLSIWYVPFEQKEYSVRQMQFRHDENTLTAYLNDLKRIPLMNEKEEYACACAAYAGDAQAKNALVRANVRFVISVARHYR
TCGLPLADLISEGNIGLLTAAERFNPARGCRFISYAACWIRQSILKALNEKARIIRLPVSRIRAAKSTYEYIPPVTSVNV
GISKTQGGNRYGKRRATMCRAVPTVEEVHIWHTPLSLDAPLGASGSRGESGDVDGLRIGDCVPDDLYAQPEEHMLACALQ
ADIAKILRLLPARDAQVIRYRFGLGGYERRSLQEIGEIFQITKERVRQIEKKALLRIRSCARQHRLDSYIA
>Mature_311_residues
MLSIWYVPFEQKEYSVRQMQFRHDENTLTAYLNDLKRIPLMNEKEEYACACAAYAGDAQAKNALVRANVRFVISVARHYR
TCGLPLADLISEGNIGLLTAAERFNPARGCRFISYAACWIRQSILKALNEKARIIRLPVSRIRAAKSTYEYIPPVTSVNV
GISKTQGGNRYGKRRATMCRAVPTVEEVHIWHTPLSLDAPLGASGSRGESGDVDGLRIGDCVPDDLYAQPEEHMLACALQ
ADIAKILRLLPARDAQVIRYRFGLGGYERRSLQEIGEIFQITKERVRQIEKKALLRIRSCARQHRLDSYIA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=253, Percent_Identity=34.3873517786561, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1789098, Length=277, Percent_Identity=31.4079422382672, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1789871, Length=280, Percent_Identity=27.5, Blast_Score=105, Evalue=5e-24,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 35114; Mature: 35114

Theoretical pI: Translated: 9.55; Mature: 9.55

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSIWYVPFEQKEYSVRQMQFRHDENTLTAYLNDLKRIPLMNEKEEYACACAAYAGDAQA
CCEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHH
KNALVRANVRFVISVARHYRTCGLPLADLISEGNIGLLTAAERFNPARGCRFISYAACWI
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEEHHHCCHHHCCHHHHHHHHHH
RQSILKALNEKARIIRLPVSRIRAAKSTYEYIPPVTSVNVGISKTQGGNRYGKRRATMCR
HHHHHHHHHHHHEEEECCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHH
AVPTVEEVHIWHTPLSLDAPLGASGSRGESGDVDGLRIGDCVPDDLYAQPEEHMLACALQ
HCCCHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHCCCCHHHHHHHHHH
ADIAKILRLLPARDAQVIRYRFGLGGYERRSLQEIGEIFQITKERVRQIEKKALLRIRSC
HHHHHHHHHCCCCCHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARQHRLDSYIA
HHHHHHHHHCC
>Mature Secondary Structure
MLSIWYVPFEQKEYSVRQMQFRHDENTLTAYLNDLKRIPLMNEKEEYACACAAYAGDAQA
CCEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHH
KNALVRANVRFVISVARHYRTCGLPLADLISEGNIGLLTAAERFNPARGCRFISYAACWI
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCEEEEEEHHHCCHHHCCHHHHHHHHHH
RQSILKALNEKARIIRLPVSRIRAAKSTYEYIPPVTSVNVGISKTQGGNRYGKRRATMCR
HHHHHHHHHHHHEEEECCHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHHHHH
AVPTVEEVHIWHTPLSLDAPLGASGSRGESGDVDGLRIGDCVPDDLYAQPEEHMLACALQ
HCCCHHHEEEEECCCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHCCCCHHHHHHHHHH
ADIAKILRLLPARDAQVIRYRFGLGGYERRSLQEIGEIFQITKERVRQIEKKALLRIRSC
HHHHHHHHHCCCCCHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARQHRLDSYIA
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11463916 [H]