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The map label for this gene is gyrB

Identifier: 189026229

GI number: 189026229

Start: 1096454

End: 1098367

Strand: Reverse

Name: gyrB

Synonym: TPASS_1006

Alternate gene names: 189026229

Gene position: 1098367-1096454 (Counterclockwise)

Preceding gene: 189026230

Following gene: 189026228

Centisome position: 96.39

GC content: 48.38

Gene sequence:

>1914_bases
ATGGGGATTGAGTACTCAGCGAGTAGCATTACTGTATTGGAAGGTCTTGAAGCGGTACGCAAGCGTCCGGGGATGTATAT
CGGCTCTACCGGTCCTAATGGATTGCACCATCTGGTGTACGAGGTGGTGGATAACTGTATCGATGAAGCCATGGCTGGGT
ACTGTGATCGTATCACCGTGGTGCTCGAACAAGGAAACGTCGTGCGTGTTGAAGACAACGGGCGAGGTATTCCTGTTGAC
GTGCACCCTCATGAGGGGGTTAGTGCGCTTGAGGTTGTACTTACTAAGTTACATGCGGGGGGGAAGTTTGACAAGAAATC
GTATAAGGTGTCGGGTGGACTCCACGGAGTTGGAGTTTCTGTGGTCAACGCGCTGTCGTTGTGGGTAGAAGTGACAGTGT
ATCGTGATGGTGCTGAGTATTATCAGAAGTTTAATGTGGGGATGCCGCTTGCTCCAGTAGAGAAGCGGGGAGTGTCGGAA
AAACGTGGCACTATTATCCGCTGGCAGGCGGACCCATCCATTTTCAAAGAAACGGTGGCCTATGATTTTGACGTACTCCT
GACGCGTTTGCGTGAACTTGCTTTTTTGAATAGCACGGTAGTTATTCAGTTGCGTGATGAGCGGTTGGCGACCGCTAAAC
AGGTTGAATTTGCGTTCGAAGGAGGTATTCGTCATTTTGTCAGTTATTTAAACCGCGGTAAATCAGTTGTGCCCGAACGT
CCTCTGTACATTGAGGGATCGAAGTCGGATGTTTTAGTGGAAGTTGCGTTGCAATATCACGATGGTTATACGGAAAACGT
GCAGTCATTTGTCAATGATATTAATACCCGTGAGGGGGGCACGCATCTTGAAGGATTTAAGTCGGCACTTACGCGTGTGG
CGAACGATTTTTTGAAAAAAAGTCCAAAGCTTGCAAAGAAGATAGAAAGGGAAGAAAAGCTCGTTGGGGAAGATGTGCGT
GCTGGATTGACAGTGGTGCTTTCTGTGAAAATTCCTGAACCCCAGTTTGAAGGGCAGACAAAGACGAAGTTGGGAAACAG
TGAGGTGCGGGGTATTGTTGATTCTTTGGTGGGGGAGCGTCTGACGCTCTATTTTGAGCAAAATCCAGGTGTGCTTACAA
AGATTCTTGAAAAGAGCATTGCAGAGGCGCAGGCGCGTCTTGCAGCACGTCGTGCAAAGGAAGCTGCGCGCAGAAAAAGT
GGAATGGATAGTTTTGGGTTGCCGGGAAAGTTGGCCGACTGTTCGCTCAAGGATCCGGCGAAGTGCGAAGTATATATTGT
GGAAGGGGATTCTGCAGGAGGTTCGGCGAAAAAAGGACGGGACAGCAAGACACAGGCCATTTTGCCTTTGTGGGGGAAGA
TGCTGAACGTGGAAAAGACACGTTTGGATAAGGTCTTGCATAACGAAAAATTACAGCCAATTATCGCAACGCTCGGTACA
GGTGTTGGCAAGGATTTTGATTTAACAAGGATTCGCTATCATAAAGTGATCATCATGGCGGATGCGGACGTGGATGGCTC
TCACATCCGTACGCTTCTTTTAACGTTCTTCTTTCGATACCTGCCGCAAATAATTGAAGCTGGTTACGTATATCTTGCGA
TGCCGCCTTTGTATCGCATTGCGTGGAGTAAAAAGGAACTGTATGTGTATAGCGACACAGAGCGTGACGAAGCGCTAGAA
AGTATCGGTAAAAAAAGTGGTGTCGCTGTGCAGCGTTATAAAGGTCTGGGGGAAATGGATGGCACTCAGCTTTGGGAGAC
AACTATGAATCCAGTGCGTCGCAAGATGATGCAGGTGGTGCTCTCAGATGCGGTGGAAGCAGACCGGGTGTTTAGTACTC
TCATGGGTGAAGATGTCGAACCGCGCCGTAAGTTTATTGAAGAGAATGCAATATATGCGCGTTTGGACGTATGA

Upstream 100 bases:

>100_bases
AGTGCGCTCGTCTGTGTGAGCAGCAAGAGTATTGCCTTTCTGTGTCTTAAAAAGGTGAATGTGGTCATAGGTATGCTGAT
GAAAAGGAGAGCGGTCAGTT

Downstream 100 bases:

>100_bases
ATGTTTTGTGCATTTGTATTTCCATGTGAGTGGTGCCGTCTGACAGGGGGAAGGATCAGTGGCGTATCAAGTGACGGCAA
CACGGTATCGGCCGCAACGT

Product: DNA gyrase, subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 637; Mature: 636

Protein sequence:

>637_residues
MGIEYSASSITVLEGLEAVRKRPGMYIGSTGPNGLHHLVYEVVDNCIDEAMAGYCDRITVVLEQGNVVRVEDNGRGIPVD
VHPHEGVSALEVVLTKLHAGGKFDKKSYKVSGGLHGVGVSVVNALSLWVEVTVYRDGAEYYQKFNVGMPLAPVEKRGVSE
KRGTIIRWQADPSIFKETVAYDFDVLLTRLRELAFLNSTVVIQLRDERLATAKQVEFAFEGGIRHFVSYLNRGKSVVPER
PLYIEGSKSDVLVEVALQYHDGYTENVQSFVNDINTREGGTHLEGFKSALTRVANDFLKKSPKLAKKIEREEKLVGEDVR
AGLTVVLSVKIPEPQFEGQTKTKLGNSEVRGIVDSLVGERLTLYFEQNPGVLTKILEKSIAEAQARLAARRAKEAARRKS
GMDSFGLPGKLADCSLKDPAKCEVYIVEGDSAGGSAKKGRDSKTQAILPLWGKMLNVEKTRLDKVLHNEKLQPIIATLGT
GVGKDFDLTRIRYHKVIIMADADVDGSHIRTLLLTFFFRYLPQIIEAGYVYLAMPPLYRIAWSKKELYVYSDTERDEALE
SIGKKSGVAVQRYKGLGEMDGTQLWETTMNPVRRKMMQVVLSDAVEADRVFSTLMGEDVEPRRKFIEENAIYARLDV

Sequences:

>Translated_637_residues
MGIEYSASSITVLEGLEAVRKRPGMYIGSTGPNGLHHLVYEVVDNCIDEAMAGYCDRITVVLEQGNVVRVEDNGRGIPVD
VHPHEGVSALEVVLTKLHAGGKFDKKSYKVSGGLHGVGVSVVNALSLWVEVTVYRDGAEYYQKFNVGMPLAPVEKRGVSE
KRGTIIRWQADPSIFKETVAYDFDVLLTRLRELAFLNSTVVIQLRDERLATAKQVEFAFEGGIRHFVSYLNRGKSVVPER
PLYIEGSKSDVLVEVALQYHDGYTENVQSFVNDINTREGGTHLEGFKSALTRVANDFLKKSPKLAKKIEREEKLVGEDVR
AGLTVVLSVKIPEPQFEGQTKTKLGNSEVRGIVDSLVGERLTLYFEQNPGVLTKILEKSIAEAQARLAARRAKEAARRKS
GMDSFGLPGKLADCSLKDPAKCEVYIVEGDSAGGSAKKGRDSKTQAILPLWGKMLNVEKTRLDKVLHNEKLQPIIATLGT
GVGKDFDLTRIRYHKVIIMADADVDGSHIRTLLLTFFFRYLPQIIEAGYVYLAMPPLYRIAWSKKELYVYSDTERDEALE
SIGKKSGVAVQRYKGLGEMDGTQLWETTMNPVRRKMMQVVLSDAVEADRVFSTLMGEDVEPRRKFIEENAIYARLDV
>Mature_636_residues
GIEYSASSITVLEGLEAVRKRPGMYIGSTGPNGLHHLVYEVVDNCIDEAMAGYCDRITVVLEQGNVVRVEDNGRGIPVDV
HPHEGVSALEVVLTKLHAGGKFDKKSYKVSGGLHGVGVSVVNALSLWVEVTVYRDGAEYYQKFNVGMPLAPVEKRGVSEK
RGTIIRWQADPSIFKETVAYDFDVLLTRLRELAFLNSTVVIQLRDERLATAKQVEFAFEGGIRHFVSYLNRGKSVVPERP
LYIEGSKSDVLVEVALQYHDGYTENVQSFVNDINTREGGTHLEGFKSALTRVANDFLKKSPKLAKKIEREEKLVGEDVRA
GLTVVLSVKIPEPQFEGQTKTKLGNSEVRGIVDSLVGERLTLYFEQNPGVLTKILEKSIAEAQARLAARRAKEAARRKSG
MDSFGLPGKLADCSLKDPAKCEVYIVEGDSAGGSAKKGRDSKTQAILPLWGKMLNVEKTRLDKVLHNEKLQPIIATLGTG
VGKDFDLTRIRYHKVIIMADADVDGSHIRTLLLTFFFRYLPQIIEAGYVYLAMPPLYRIAWSKKELYVYSDTERDEALES
IGKKSGVAVQRYKGLGEMDGTQLWETTMNPVRRKMMQVVLSDAVEADRVFSTLMGEDVEPRRKFIEENAIYARLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain

Homologues:

Organism=Homo sapiens, GI19913408, Length=608, Percent_Identity=25.4934210526316, Blast_Score=147, Evalue=3e-35,
Organism=Homo sapiens, GI19913406, Length=609, Percent_Identity=25.1231527093596, Blast_Score=139, Evalue=9e-33,
Organism=Escherichia coli, GI48994957, Length=565, Percent_Identity=51.858407079646, Blast_Score=563, Evalue=1e-161,
Organism=Escherichia coli, GI1789408, Length=646, Percent_Identity=39.3188854489164, Blast_Score=427, Evalue=1e-120,
Organism=Caenorhabditis elegans, GI17535065, Length=597, Percent_Identity=24.7906197654941, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI212645845, Length=543, Percent_Identity=25.2302025782689, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=193, Percent_Identity=27.979274611399, Blast_Score=83, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6324241, Length=633, Percent_Identity=24.8025276461295, Blast_Score=133, Evalue=7e-32,
Organism=Drosophila melanogaster, GI17136538, Length=573, Percent_Identity=27.0506108202443, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GYRB_TREPA (O08399)

Other databases:

- EMBL:   U78094
- EMBL:   AE000520
- PIR:   H71254
- RefSeq:   NP_219443.1
- ProteinModelPortal:   O08399
- IntAct:   O08399
- GeneID:   2610807
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP1006
- NMPDR:   fig|243276.1.peg.1001
- TIGR:   TP_1006
- HOGENOM:   HBG304889
- OMA:   DCSSRDP
- ProtClustDB:   PRK05644
- BioCyc:   TPAL243276:TP_1006-MONOMER
- BRENDA:   5.99.1.3
- GO:   GO:0005694
- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171
- Gene3D:   G3DSA:3.30.565.10
- Gene3D:   G3DSA:3.30.230.10
- Gene3D:   G3DSA:3.40.50.670
- PRINTS:   PR00418
- SMART:   SM00387
- SMART:   SM00433
- TIGRFAMs:   TIGR01059

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim; SSF55874 ATP_bd_ATPase; SSF54211 Ribosomal_S5_D2-typ_fold; SSF56719 Topo_IIA_cen

EC number: =5.99.1.3

Molecular weight: Translated: 70911; Mature: 70780

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIEYSASSITVLEGLEAVRKRPGMYIGSTGPNGLHHLVYEVVDNCIDEAMAGYCDRITV
CCCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEE
VLEQGNVVRVEDNGRGIPVDVHPHEGVSALEVVLTKLHAGGKFDKKSYKVSGGLHGVGVS
EEECCCEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHH
VVNALSLWVEVTVYRDGAEYYQKFNVGMPLAPVEKRGVSEKRGTIIRWQADPSIFKETVA
HHHHHHHHEEEEEEECCHHHHHHHCCCCCCCCHHHCCCCHHCCEEEEEECCHHHHHHHHH
YDFDVLLTRLRELAFLNSTVVIQLRDERLATAKQVEFAFEGGIRHFVSYLNRGKSVVPER
HHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
PLYIEGSKSDVLVEVALQYHDGYTENVQSFVNDINTREGGTHLEGFKSALTRVANDFLKK
CEEEECCCCCEEEEEEEHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
SPKLAKKIEREEKLVGEDVRAGLTVVLSVKIPEPQFEGQTKTKLGNSEVRGIVDSLVGER
CHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHCCCE
LTLYFEQNPGVLTKILEKSIAEAQARLAARRAKEAARRKSGMDSFGLPGKLADCSLKDPA
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
KCEVYIVEGDSAGGSAKKGRDSKTQAILPLWGKMLNVEKTRLDKVLHNEKLQPIIATLGT
CEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHCC
GVGKDFDLTRIRYHKVIIMADADVDGSHIRTLLLTFFFRYLPQIIEAGYVYLAMPPLYRI
CCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHH
AWSKKELYVYSDTERDEALESIGKKSGVAVQRYKGLGEMDGTQLWETTMNPVRRKMMQVV
EECCCEEEEEECCCHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
LSDAVEADRVFSTLMGEDVEPRRKFIEENAIYARLDV
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEECC
>Mature Secondary Structure 
GIEYSASSITVLEGLEAVRKRPGMYIGSTGPNGLHHLVYEVVDNCIDEAMAGYCDRITV
CCCCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCEEEE
VLEQGNVVRVEDNGRGIPVDVHPHEGVSALEVVLTKLHAGGKFDKKSYKVSGGLHGVGVS
EEECCCEEEECCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCHHHHH
VVNALSLWVEVTVYRDGAEYYQKFNVGMPLAPVEKRGVSEKRGTIIRWQADPSIFKETVA
HHHHHHHHEEEEEEECCHHHHHHHCCCCCCCCHHHCCCCHHCCEEEEEECCHHHHHHHHH
YDFDVLLTRLRELAFLNSTVVIQLRDERLATAKQVEFAFEGGIRHFVSYLNRGKSVVPER
HHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCC
PLYIEGSKSDVLVEVALQYHDGYTENVQSFVNDINTREGGTHLEGFKSALTRVANDFLKK
CEEEECCCCCEEEEEEEHHCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHC
SPKLAKKIEREEKLVGEDVRAGLTVVLSVKIPEPQFEGQTKTKLGNSEVRGIVDSLVGER
CHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCHHHCCCCHHHHHHHHHHCCCE
LTLYFEQNPGVLTKILEKSIAEAQARLAARRAKEAARRKSGMDSFGLPGKLADCSLKDPA
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
KCEVYIVEGDSAGGSAKKGRDSKTQAILPLWGKMLNVEKTRLDKVLHNEKLQPIIATLGT
CEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHCC
GVGKDFDLTRIRYHKVIIMADADVDGSHIRTLLLTFFFRYLPQIIEAGYVYLAMPPLYRI
CCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHH
AWSKKELYVYSDTERDEALESIGKKSGVAVQRYKGLGEMDGTQLWETTMNPVRRKMMQVV
EECCCEEEEEECCCHHHHHHHHHHHCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
LSDAVEADRVFSTLMGEDVEPRRKFIEENAIYARLDV
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9252582; 9665876