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The map label for this gene is nusA [H]

Identifier: 189026115

GI number: 189026115

Start: 971918

End: 973375

Strand: Reverse

Name: nusA [H]

Synonym: TPASS_0892

Alternate gene names: 189026115

Gene position: 973375-971918 (Counterclockwise)

Preceding gene: 189026116

Following gene: 189026114

Centisome position: 85.42

GC content: 49.66

Gene sequence:

>1458_bases
ATGTTCGGCGTCAGTAACGATGACATTAGAAAGTATGCGCAGGAGAAGGGGCTTGATGAAGACTTTGCCTTTAAAATCGT
CGAGCAAACACTGAAGGCCGCTTATAAGACTACATTTAAGACAGATGAAAACGCCGTCGTTACCTTTGGTGAGGAGCGGG
TGTGTATCTATGCGCGCAAGCGCGTGGTTGAAGAGGTGTACGACCGCGTCTCGGAAGTGGATTTGTCTACGGCACTTGAG
CTTGATCCCACTACTTCTTTAGATAGCGAAGTGCTGGTGGAGCTTGAGTCCGAAGATTTTAAGCGTGGATCTGTGCAGGC
TGCCGTCCAGCGTATCACTGAGCTGAGCAGAGAAATTCAAAAGGACGCTCTGTATGCTGAGTACAAGAGCAAAGAAGGAG
AGATTATCGTTGGCTACTACCAACGCGCGCGAAACGAGCATATCTACGTTGACCTAGGAAAAGTTGAGGGCCTGATGCCA
AAGTCGCACCAGCTGCCCCAGGATGATTATCGTCAAAACGACCGCATTAAGTCGCTTGTGCGTGAGGTGCGCAAACATCC
AAAGTCGAGCGTTGTCCAGCTCATTCTTTCACGAACTGACTCTGCTTTTGTAAAAGAGCTGCTCGCCGTGGAGGTGCCGG
AGATCTACGACGGTATTGTTGAGGTGGCAAAAATAGTGCGGGAGCCAGGGTACCGTACAAAGATCGCCGTCACCAGTAGG
CGTGATGATGTGGATCCTGTTGGTGCCTGCGTAGGTCCTCGGGGCATACGCATCCGCATGGTTATTAAAGAATTGAATGA
CGAGAAGATAGATGTGCTTGAGTATTCTCCGGATCCAGTTATTTTCATCAAAAATGCGCTTTCTCCTGCTGAGGTGCTGA
ACGTCGTGGTACTTGATGAGGAGAAGCGTTCTGCACTTGCCATTGTTGCTGAAAGCCAGCTGTCTATCGCGATAGGAAAG
CAAGGTTTGAACGTGCGTTTAGCGAATCGGCTTGTGGACTGGAATATCGATGTGAAGACAGAGAGTCAGTTTGAAGAGAT
GGATGTGTACACTGACACGCGTCGTGCGGCAGAAAATCTTTTTGATAACGATTATCAAGAAGAGTCTGAGTTTTCTTCAT
ACGTGGGATTTACGCCGGAGCTCATTAAGATTCTGCAGGACAACGGTATCCAAGACGTACAGACTTTGGTAGATTTGGGC
GAGGAAGGCTTGCGTGCGCTTGAGGGCATGGACGAGGCGCACGTACAAGAATTGCTCGCCGCCATTGAGGAGAATTTTGA
AGTTGTCGAGGAGGGGGAGGAGGCTTCAGTTACATCTTCTCCCGGGACTGGTGGTGATGAGGATCAGGCGTTGCAGTGTC
CTGAGTGTGGGGTGCGCATTACTACTGACATGAGTGAGTGTCCTCACTGTGGTATTGGCCTCAGCTTTGAGTTTGAATAC
GAAGAGAACGAGGAATAG

Upstream 100 bases:

>100_bases
CCGTATGTTCAGCTAACAAAAGCGCAGTTACATCCTGCAGTCCGCGCTTGAAAGGGCTTTTGGTCTGCACCTTCGCCCAA
AATCGCCTAAGGAGCCGCCT

Downstream 100 bases:

>100_bases
GAGAGCTATGACCCTACGAGACAATACGCCTAAAGACACTTCCCGTGTTGCTAGTGAGCAGGCTGTGCGTTCACCGGTGA
CGTCTGGTCAAACTACCTCT

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MFGVSNDDIRKYAQEKGLDEDFAFKIVEQTLKAAYKTTFKTDENAVVTFGEERVCIYARKRVVEEVYDRVSEVDLSTALE
LDPTTSLDSEVLVELESEDFKRGSVQAAVQRITELSREIQKDALYAEYKSKEGEIIVGYYQRARNEHIYVDLGKVEGLMP
KSHQLPQDDYRQNDRIKSLVREVRKHPKSSVVQLILSRTDSAFVKELLAVEVPEIYDGIVEVAKIVREPGYRTKIAVTSR
RDDVDPVGACVGPRGIRIRMVIKELNDEKIDVLEYSPDPVIFIKNALSPAEVLNVVVLDEEKRSALAIVAESQLSIAIGK
QGLNVRLANRLVDWNIDVKTESQFEEMDVYTDTRRAAENLFDNDYQEESEFSSYVGFTPELIKILQDNGIQDVQTLVDLG
EEGLRALEGMDEAHVQELLAAIEENFEVVEEGEEASVTSSPGTGGDEDQALQCPECGVRITTDMSECPHCGIGLSFEFEY
EENEE

Sequences:

>Translated_485_residues
MFGVSNDDIRKYAQEKGLDEDFAFKIVEQTLKAAYKTTFKTDENAVVTFGEERVCIYARKRVVEEVYDRVSEVDLSTALE
LDPTTSLDSEVLVELESEDFKRGSVQAAVQRITELSREIQKDALYAEYKSKEGEIIVGYYQRARNEHIYVDLGKVEGLMP
KSHQLPQDDYRQNDRIKSLVREVRKHPKSSVVQLILSRTDSAFVKELLAVEVPEIYDGIVEVAKIVREPGYRTKIAVTSR
RDDVDPVGACVGPRGIRIRMVIKELNDEKIDVLEYSPDPVIFIKNALSPAEVLNVVVLDEEKRSALAIVAESQLSIAIGK
QGLNVRLANRLVDWNIDVKTESQFEEMDVYTDTRRAAENLFDNDYQEESEFSSYVGFTPELIKILQDNGIQDVQTLVDLG
EEGLRALEGMDEAHVQELLAAIEENFEVVEEGEEASVTSSPGTGGDEDQALQCPECGVRITTDMSECPHCGIGLSFEFEY
EENEE
>Mature_485_residues
MFGVSNDDIRKYAQEKGLDEDFAFKIVEQTLKAAYKTTFKTDENAVVTFGEERVCIYARKRVVEEVYDRVSEVDLSTALE
LDPTTSLDSEVLVELESEDFKRGSVQAAVQRITELSREIQKDALYAEYKSKEGEIIVGYYQRARNEHIYVDLGKVEGLMP
KSHQLPQDDYRQNDRIKSLVREVRKHPKSSVVQLILSRTDSAFVKELLAVEVPEIYDGIVEVAKIVREPGYRTKIAVTSR
RDDVDPVGACVGPRGIRIRMVIKELNDEKIDVLEYSPDPVIFIKNALSPAEVLNVVVLDEEKRSALAIVAESQLSIAIGK
QGLNVRLANRLVDWNIDVKTESQFEEMDVYTDTRRAAENLFDNDYQEESEFSSYVGFTPELIKILQDNGIQDVQTLVDLG
EEGLRALEGMDEAHVQELLAAIEENFEVVEEGEEASVTSSPGTGGDEDQALQCPECGVRITTDMSECPHCGIGLSFEFEY
EENEE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=468, Percent_Identity=29.2735042735043, Blast_Score=194, Evalue=9e-51,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 54678; Mature: 54678

Theoretical pI: Translated: 4.21; Mature: 4.21

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGVSNDDIRKYAQEKGLDEDFAFKIVEQTLKAAYKTTFKTDENAVVTFGEERVCIYARK
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEHHHHHH
RVVEEVYDRVSEVDLSTALELDPTTSLDSEVLVELESEDFKRGSVQAAVQRITELSREIQ
HHHHHHHHHHHHCCHHHEEECCCCCCCCCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHH
KDALYAEYKSKEGEIIVGYYQRARNEHIYVDLGKVEGLMPKSHQLPQDDYRQNDRIKSLV
HHHHHHHHCCCCCCEEEEEHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHCCHHHHHHH
REVRKHPKSSVVQLILSRTDSAFVKELLAVEVPEIYDGIVEVAKIVREPGYRTKIAVTSR
HHHHHCCHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEECC
RDDVDPVGACVGPRGIRIRMVIKELNDEKIDVLEYSPDPVIFIKNALSPAEVLNVVVLDE
CCCCCCHHHCCCCCCCEEEEEEHHCCCCCEEEEEECCCCEEEEECCCCHHHHHEEEEECC
EKRSALAIVAESQLSIAIGKQGLNVRLANRLVDWNIDVKTESQFEEMDVYTDTRRAAENL
CCCCEEEEEECCCEEEEECCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
FDNDYQEESEFSSYVGFTPELIKILQDNGIQDVQTLVDLGEEGLRALEGMDEAHVQELLA
HCCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHH
AIEENFEVVEEGEEASVTSSPGTGGDEDQALQCPECGVRITTDMSECPHCGIGLSFEFEY
HHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHCCCCCCCCEEEECC
EENEE
CCCCC
>Mature Secondary Structure
MFGVSNDDIRKYAQEKGLDEDFAFKIVEQTLKAAYKTTFKTDENAVVTFGEERVCIYARK
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEHHHHHH
RVVEEVYDRVSEVDLSTALELDPTTSLDSEVLVELESEDFKRGSVQAAVQRITELSREIQ
HHHHHHHHHHHHCCHHHEEECCCCCCCCCHHEEEECCCCCCCHHHHHHHHHHHHHHHHHH
KDALYAEYKSKEGEIIVGYYQRARNEHIYVDLGKVEGLMPKSHQLPQDDYRQNDRIKSLV
HHHHHHHHCCCCCCEEEEEHHHHCCCEEEEEECCCCCCCCCCCCCCCHHHHCCHHHHHHH
REVRKHPKSSVVQLILSRTDSAFVKELLAVEVPEIYDGIVEVAKIVREPGYRTKIAVTSR
HHHHHCCHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEEECC
RDDVDPVGACVGPRGIRIRMVIKELNDEKIDVLEYSPDPVIFIKNALSPAEVLNVVVLDE
CCCCCCHHHCCCCCCCEEEEEEHHCCCCCEEEEEECCCCEEEEECCCCHHHHHEEEEECC
EKRSALAIVAESQLSIAIGKQGLNVRLANRLVDWNIDVKTESQFEEMDVYTDTRRAAENL
CCCCEEEEEECCCEEEEECCCCCCEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
FDNDYQEESEFSSYVGFTPELIKILQDNGIQDVQTLVDLGEEGLRALEGMDEAHVQELLA
HCCCCHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHH
AIEENFEVVEEGEEASVTSSPGTGGDEDQALQCPECGVRITTDMSECPHCGIGLSFEFEY
HHHHCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHCCCCCCCCEEEECC
EENEE
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]