Click here to switch to the map view.

The map label for this gene is metG

Identifier: 189026023

GI number: 189026023

Start: 865516

End: 867951

Strand: Reverse

Name: metG

Synonym: TPASS_0798

Alternate gene names: 189026023

Gene position: 867951-865516 (Counterclockwise)

Preceding gene: 189026026

Following gene: 189026022

Centisome position: 76.17

GC content: 53.86

Gene sequence:

>2436_bases
ATGACGCGAAAATTAATCACCGCCGCACTCCCCTATGTGAACAACGTTCCACATTTGGGAAATCTTATCCAGGGTCTTTC
TGCAGACGTTTTCGCACGTTTCTGTCGGATGCGCGGCTATCACACGTGTTTTGTATGTGGTACCGACGAATACGGCACGG
CAAGCGAAACCCGTGCGGCAGAACAAGGTCTCAGTCCTGCACAATTGTGTGCGCACTACCATGCACTGCATCGCGACATC
TATCAGTGGTTTGATCTGTCCTTCGATTATTTTGGGCGCACTACAAGCGATGCGCATACTGAGCTTACGCAAGCGTTGTT
TCGTCATTTGGATGCGCGGGGTTTTATCAGTGAACATGAAAGTGCGCAGGCGTACTGTCTGCACTGTGCACGGTTTCTTG
CTGATCGCTATTTGCGCGGTACCTGTCCCCATTGCCGTAATGCTGAGGCGCGTGCTGACCAGTGCGAGCACTGTGGAGTG
CTCCTTGAGCCGGAAACGCTCCTGAATGCGCGCTGTGTGAGCTGTGGCACGGCGCCGGAGTTTCGCCCTACGCGTCATTT
GTATTTAAATTTGCCTGCACTGGAAAAAGCCTACCGCTCGTGGTTTTGCACCACGAATCATCTGTGGACTAAAAACGCGG
TGCGTATGACTGAAGGTTGGCTACGTACGGGATTGCAGGAGCGTGCGATCACGCGCGATCTGCGCTGGGGGGTGCCAGTT
CCCAAAGCAGGATTTGAGCAGAAGGTATTTTATGTGTGGTTCGATGCGCCAGTCGGTTACATTTCCATTACTAAGTGCGG
CACAGAGGCAGCTTCCTCGCAAGAAGGGGGGGGGACCGACGATGGCGTGAAAGAAAAATGGCAGTCTTGGTGGCTTGATC
AGCAGGATGTGGAGTTGGTCCAGTTTGTGGGGAAGGACAATATTCCCTTTCATACGCTGTTTTTCCCCTGCATGCTCATC
GGTTCCGGGCAGCGGTGGACGATGCTTACGCGTCTTTCTGCGACGGAGTATTTGAATTACGAAGGGGGGAAGTTTTCTAA
GTCTTTAGGGGTGGGCGTTTTTGGTTCGGATGCAAAAGAATCGGGCATTCCCTCAGATCTGTGGCGTTTTTATCTCCTGT
ACCATAGACCGGAAAAAAGCGATGCGCACTTTACCTGGCATGAGTTTCAGGAGCGTGTAAACAGTGAGTTGATTGGTAAT
CTGTGTAATCTGGTCAATCGTACGCTCACCTTTGTGGCGCGTACGTACGGGGGCGTGGTCCCTGCGCAAGATGGAGCGCG
CAGCACCCGTGCGCAGGTGATGGAAGAAACGCTTGCGCTCCGCGAAGCGGTGCGGAATACTGCAAAGCGCATGACAGATT
TAATGGAGCAGGTACAGTTGCGAGAAGCGTTTAGAGAAGTGTTTGCGCTCTCAGCGCGTGCGAATAAGGCGTTGCAGGAT
GGTGCACCGTGGAAAACGCGGGCGCAGGACCGTGAACGTGCAGACGCCTTGATGCGTGAGTTATGCTATGTGATTCGGGA
TGTGCTGATTTTAGCGCATCCTTTTTTGCCGTGGTACACGCAGCAAGCGGCCCGATTTTTGGGTGTTCAGTTGTCTTCCT
GTGCACCAGAGGGGGGAGGAGCTGTGTGTGCTGCGAAGAAAGACGCGGATACGGCGCAGCCGGACACAGTGCAACCGACC
CTCCGATGGTCAGACGTGGGAGAACGCAAGGGTTTAACGCAGGTGCATCCGCCGGTGATTTTATTCCGTCCGTTGGAGAC
GGAAACTATTGCTGCGTATCGTGCCCGCTATGCTGGAACAGCCAGGGATGGGGCAGGAGTGAGCGTACCGCGCACTGCAC
AGATGCCCACGGGAATGAATAAGAAAGAGACAGACGCTCAACAAAAGAAGGAGGAGCGTGAAATGCCCCCTCCCTCAGAT
ACTGCACGGTTATCTGCATTTTTTTCTGAGCGCGTTGTACTGAAAGTAGCACGAGTGTTGCAGGTGGAGCGTCATCCGAA
TGCGGATATGCTTTTTGTTGAAACATTAGATGATGGCTCTGGCGTTGAGCGCGTTATTGTTTCTGGTCTTGTGCCTTATA
TGGCTGCAGATGCGTTGCGTGGTGCGCACGTGCTTATTGTGGATAATCTGCAGCCGCGCTCACTGCGTGGGGTACGGTCT
TGCGGCATGCTGTTGGCCGCAGAGTATGTAGATGCGCAGGGCACAAAGGCAATTGAATTGGTGCAGGCGCCATGGGCTCT
GCCCGGTGAACGCGCAACACTTGCGAGTGCGCCGCCGGTCATTACACCGCACGGGTCTGCCGTTATCGATGCGGACGCTT
TTTTTTCTGTGCCTATTCGTGTGGTAAATTATGCAGTAGAAGTTGCAGGTGAGCCGCTCATGGTTGGAGGAAGGCCACTG
GTAATGCAGCGAGTGAAAGAGGGAACTGTCGGCTAG

Upstream 100 bases:

>100_bases
TGCACCGGATGCTCCTTGCGTCTGCCTGCCCTTTTGGGCAACACTTGTTGCCAGAAACCTGTCTTCACGAAGTCTTGTTT
AAATCAGAGTAGGAAACGCT

Downstream 100 bases:

>100_bases
GAATATTCACAGAGCATTTGGTTTTCCGTGTCGGATAGGGGGAGCGCAGCATGAACGTGGGATTTTTGGGTTTTGGAGCA
ATGGGACGGGCGCTGGCAGA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS

Number of amino acids: Translated: 811; Mature: 810

Protein sequence:

>811_residues
MTRKLITAALPYVNNVPHLGNLIQGLSADVFARFCRMRGYHTCFVCGTDEYGTASETRAAEQGLSPAQLCAHYHALHRDI
YQWFDLSFDYFGRTTSDAHTELTQALFRHLDARGFISEHESAQAYCLHCARFLADRYLRGTCPHCRNAEARADQCEHCGV
LLEPETLLNARCVSCGTAPEFRPTRHLYLNLPALEKAYRSWFCTTNHLWTKNAVRMTEGWLRTGLQERAITRDLRWGVPV
PKAGFEQKVFYVWFDAPVGYISITKCGTEAASSQEGGGTDDGVKEKWQSWWLDQQDVELVQFVGKDNIPFHTLFFPCMLI
GSGQRWTMLTRLSATEYLNYEGGKFSKSLGVGVFGSDAKESGIPSDLWRFYLLYHRPEKSDAHFTWHEFQERVNSELIGN
LCNLVNRTLTFVARTYGGVVPAQDGARSTRAQVMEETLALREAVRNTAKRMTDLMEQVQLREAFREVFALSARANKALQD
GAPWKTRAQDRERADALMRELCYVIRDVLILAHPFLPWYTQQAARFLGVQLSSCAPEGGGAVCAAKKDADTAQPDTVQPT
LRWSDVGERKGLTQVHPPVILFRPLETETIAAYRARYAGTARDGAGVSVPRTAQMPTGMNKKETDAQQKKEEREMPPPSD
TARLSAFFSERVVLKVARVLQVERHPNADMLFVETLDDGSGVERVIVSGLVPYMAADALRGAHVLIVDNLQPRSLRGVRS
CGMLLAAEYVDAQGTKAIELVQAPWALPGERATLASAPPVITPHGSAVIDADAFFSVPIRVVNYAVEVAGEPLMVGGRPL
VMQRVKEGTVG

Sequences:

>Translated_811_residues
MTRKLITAALPYVNNVPHLGNLIQGLSADVFARFCRMRGYHTCFVCGTDEYGTASETRAAEQGLSPAQLCAHYHALHRDI
YQWFDLSFDYFGRTTSDAHTELTQALFRHLDARGFISEHESAQAYCLHCARFLADRYLRGTCPHCRNAEARADQCEHCGV
LLEPETLLNARCVSCGTAPEFRPTRHLYLNLPALEKAYRSWFCTTNHLWTKNAVRMTEGWLRTGLQERAITRDLRWGVPV
PKAGFEQKVFYVWFDAPVGYISITKCGTEAASSQEGGGTDDGVKEKWQSWWLDQQDVELVQFVGKDNIPFHTLFFPCMLI
GSGQRWTMLTRLSATEYLNYEGGKFSKSLGVGVFGSDAKESGIPSDLWRFYLLYHRPEKSDAHFTWHEFQERVNSELIGN
LCNLVNRTLTFVARTYGGVVPAQDGARSTRAQVMEETLALREAVRNTAKRMTDLMEQVQLREAFREVFALSARANKALQD
GAPWKTRAQDRERADALMRELCYVIRDVLILAHPFLPWYTQQAARFLGVQLSSCAPEGGGAVCAAKKDADTAQPDTVQPT
LRWSDVGERKGLTQVHPPVILFRPLETETIAAYRARYAGTARDGAGVSVPRTAQMPTGMNKKETDAQQKKEEREMPPPSD
TARLSAFFSERVVLKVARVLQVERHPNADMLFVETLDDGSGVERVIVSGLVPYMAADALRGAHVLIVDNLQPRSLRGVRS
CGMLLAAEYVDAQGTKAIELVQAPWALPGERATLASAPPVITPHGSAVIDADAFFSVPIRVVNYAVEVAGEPLMVGGRPL
VMQRVKEGTVG
>Mature_810_residues
TRKLITAALPYVNNVPHLGNLIQGLSADVFARFCRMRGYHTCFVCGTDEYGTASETRAAEQGLSPAQLCAHYHALHRDIY
QWFDLSFDYFGRTTSDAHTELTQALFRHLDARGFISEHESAQAYCLHCARFLADRYLRGTCPHCRNAEARADQCEHCGVL
LEPETLLNARCVSCGTAPEFRPTRHLYLNLPALEKAYRSWFCTTNHLWTKNAVRMTEGWLRTGLQERAITRDLRWGVPVP
KAGFEQKVFYVWFDAPVGYISITKCGTEAASSQEGGGTDDGVKEKWQSWWLDQQDVELVQFVGKDNIPFHTLFFPCMLIG
SGQRWTMLTRLSATEYLNYEGGKFSKSLGVGVFGSDAKESGIPSDLWRFYLLYHRPEKSDAHFTWHEFQERVNSELIGNL
CNLVNRTLTFVARTYGGVVPAQDGARSTRAQVMEETLALREAVRNTAKRMTDLMEQVQLREAFREVFALSARANKALQDG
APWKTRAQDRERADALMRELCYVIRDVLILAHPFLPWYTQQAARFLGVQLSSCAPEGGGAVCAAKKDADTAQPDTVQPTL
RWSDVGERKGLTQVHPPVILFRPLETETIAAYRARYAGTARDGAGVSVPRTAQMPTGMNKKETDAQQKKEEREMPPPSDT
ARLSAFFSERVVLKVARVLQVERHPNADMLFVETLDDGSGVERVIVSGLVPYMAADALRGAHVLIVDNLQPRSLRGVRSC
GMLLAAEYVDAQGTKAIELVQAPWALPGERATLASAPPVITPHGSAVIDADAFFSVPIRVVNYAVEVAGEPLMVGGRPLV
MQRVKEGTVG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain

Homologues:

Organism=Homo sapiens, GI14043022, Length=634, Percent_Identity=41.3249211356467, Blast_Score=503, Evalue=1e-142,
Organism=Homo sapiens, GI24308436, Length=498, Percent_Identity=25.5020080321285, Blast_Score=128, Evalue=2e-29,
Organism=Homo sapiens, GI4507947, Length=90, Percent_Identity=37.7777777777778, Blast_Score=75, Evalue=3e-13,
Organism=Escherichia coli, GI1788432, Length=733, Percent_Identity=27.012278308322, Blast_Score=278, Evalue=8e-76,
Organism=Caenorhabditis elegans, GI17540794, Length=646, Percent_Identity=40.4024767801858, Blast_Score=504, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI212645096, Length=530, Percent_Identity=24.5283018867925, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71992781, Length=294, Percent_Identity=27.5510204081633, Blast_Score=96, Evalue=9e-20,
Organism=Saccharomyces cerevisiae, GI6321704, Length=528, Percent_Identity=44.6969696969697, Blast_Score=488, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6321610, Length=545, Percent_Identity=23.4862385321101, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24655311, Length=603, Percent_Identity=41.4593698175788, Blast_Score=472, Evalue=1e-133,
Organism=Drosophila melanogaster, GI21358483, Length=542, Percent_Identity=24.9077490774908, Blast_Score=110, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24651924, Length=101, Percent_Identity=33.6633663366337, Blast_Score=67, Evalue=7e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): SYM_TREPA (O83776)

Other databases:

- EMBL:   AE000520
- PIR:   E71281
- RefSeq:   NP_219235.1
- ProteinModelPortal:   O83776
- SMR:   O83776
- IntAct:   O83776
- GeneID:   2610813
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0798
- NMPDR:   fig|243276.1.peg.794
- TIGR:   TP_0798
- HOGENOM:   HBG721667
- OMA:   SAYQPEQ
- ProtClustDB:   PRK00133
- BioCyc:   TPAL243276:TP_0798-MONOMER
- BRENDA:   6.1.1.10
- GO:   GO:0005737
- HAMAP:   MF_00098
- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR01041
- TIGRFAMs:   TIGR00398

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind; SSF50249 Nucleic_acid_OB; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.10

Molecular weight: Translated: 90418; Mature: 90287

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS00178 AA_TRNA_LIGASE_I; PS50886 TRBD

Important sites: BINDING 347-347

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKLITAALPYVNNVPHLGNLIQGLSADVFARFCRMRGYHTCFVCGTDEYGTASETRAA
CCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHH
EQGLSPAQLCAHYHALHRDIYQWFDLSFDYFGRTTSDAHTELTQALFRHLDARGFISEHE
HHCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHCCC
SAQAYCLHCARFLADRYLRGTCPHCRNAEARADQCEHCGVLLEPETLLNARCVSCGTAPE
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCEEECCHHHHHHHHHCCCCCCC
FRPTRHLYLNLPALEKAYRSWFCTTNHLWTKNAVRMTEGWLRTGLQERAITRDLRWGVPV
CCCCCEEEEECHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHCHHHHHHHHHHHCCCCC
PKAGFEQKVFYVWFDAPVGYISITKCGTEAASSQEGGGTDDGVKEKWQSWWLDQQDVELV
CCCCCCCEEEEEEECCCCCEEEEEECCCHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHH
QFVGKDNIPFHTLFFPCMLIGSGQRWTMLTRLSATEYLNYEGGKFSKSLGVGVFGSDAKE
HHHCCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCCCCHHHCCCCEECCCCCC
SGIPSDLWRFYLLYHRPEKSDAHFTWHEFQERVNSELIGNLCNLVNRTLTFVARTYGGVV
CCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PAQDGARSTRAQVMEETLALREAVRNTAKRMTDLMEQVQLREAFREVFALSARANKALQD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GAPWKTRAQDRERADALMRELCYVIRDVLILAHPFLPWYTQQAARFLGVQLSSCAPEGGG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHCCCCCCC
AVCAAKKDADTAQPDTVQPTLRWSDVGERKGLTQVHPPVILFRPLETETIAAYRARYAGT
EEEEECCCCCCCCCCCCCCCEEECCCHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHCCC
ARDGAGVSVPRTAQMPTGMNKKETDAQQKKEEREMPPPSDTARLSAFFSERVVLKVARVL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
QVERHPNADMLFVETLDDGSGVERVIVSGLVPYMAADALRGAHVLIVDNLQPRSLRGVRS
HHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHH
CGMLLAAEYVDAQGTKAIELVQAPWALPGERATLASAPPVITPHGSAVIDADAFFSVPIR
HHHHHHHHHHCCCCCCHHHEECCCCCCCCCCCCCCCCCCEECCCCCEEEECCHHHHHHHH
VVNYAVEVAGEPLMVGGRPLVMQRVKEGTVG
HHHHHHHHCCCEEEECCCHHHHHHHHCCCCC
>Mature Secondary Structure 
TRKLITAALPYVNNVPHLGNLIQGLSADVFARFCRMRGYHTCFVCGTDEYGTASETRAA
CCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHH
EQGLSPAQLCAHYHALHRDIYQWFDLSFDYFGRTTSDAHTELTQALFRHLDARGFISEHE
HHCCCHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHHCCCCCCHHCCC
SAQAYCLHCARFLADRYLRGTCPHCRNAEARADQCEHCGVLLEPETLLNARCVSCGTAPE
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCEEECCHHHHHHHHHCCCCCCC
FRPTRHLYLNLPALEKAYRSWFCTTNHLWTKNAVRMTEGWLRTGLQERAITRDLRWGVPV
CCCCCEEEEECHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHHCHHHHHHHHHHHCCCCC
PKAGFEQKVFYVWFDAPVGYISITKCGTEAASSQEGGGTDDGVKEKWQSWWLDQQDVELV
CCCCCCCEEEEEEECCCCCEEEEEECCCHHCCCCCCCCCCHHHHHHHHHHCCCCHHHHHH
QFVGKDNIPFHTLFFPCMLIGSGQRWTMLTRLSATEYLNYEGGKFSKSLGVGVFGSDAKE
HHHCCCCCCHHHHHHHHHHHCCCCCEEEEEHHHHHHHCCCCCCCCHHHCCCCEECCCCCC
SGIPSDLWRFYLLYHRPEKSDAHFTWHEFQERVNSELIGNLCNLVNRTLTFVARTYGGVV
CCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PAQDGARSTRAQVMEETLALREAVRNTAKRMTDLMEQVQLREAFREVFALSARANKALQD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GAPWKTRAQDRERADALMRELCYVIRDVLILAHPFLPWYTQQAARFLGVQLSSCAPEGGG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHCCCCCCC
AVCAAKKDADTAQPDTVQPTLRWSDVGERKGLTQVHPPVILFRPLETETIAAYRARYAGT
EEEEECCCCCCCCCCCCCCCEEECCCHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHCCC
ARDGAGVSVPRTAQMPTGMNKKETDAQQKKEEREMPPPSDTARLSAFFSERVVLKVARVL
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
QVERHPNADMLFVETLDDGSGVERVIVSGLVPYMAADALRGAHVLIVDNLQPRSLRGVRS
HHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHH
CGMLLAAEYVDAQGTKAIELVQAPWALPGERATLASAPPVITPHGSAVIDADAFFSVPIR
HHHHHHHHHHCCCCCCHHHEECCCCCCCCCCCCCCCCCCEECCCCCEEEECCHHHHHHHH
VVNYAVEVAGEPLMVGGRPLVMQRVKEGTVG
HHHHHHHHCCCEEEECCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876