Click here to switch to the map view.

The map label for this gene is def

Identifier: 189025982

GI number: 189025982

Start: 822741

End: 823229

Strand: Reverse

Name: def

Synonym: TPASS_0757

Alternate gene names: 189025982

Gene position: 823229-822741 (Counterclockwise)

Preceding gene: 189025983

Following gene: 189025981

Centisome position: 72.25

GC content: 55.42

Gene sequence:

>489_bases
GTGGAGCTTAAGTTTTTAGGTGAGCCGTGCCTGACAACGGTTTCCGAGCCGGTTTCGGAGGTGGACGAGCAGCTGCGCGC
GTTCATCTCGGGTATGTTTCGTGTGATGCGTGGGGCAGGCGGTGTGGGGCTTGCGGCGCCGCAGGTAGGGCGTACCGTCC
GCGTGTTTGTAGTAGATGTTGAGCACCACGTCCGCGCTTTTATCAATCCCCAGATCACTGCCGCGTCTGAAGAGCAATCT
TCCTACGAGGAAGGCTGTTTGAGCATTCCTCACATTTATGAAAGGGTGTTGCGTCCGCGTCGGGTGAGTGTGCAGTACCT
CGACGAGAATGGTAAACGTTGTGCCGTTGATGCAGATGGTATTCTGGCAAGAGTGATCCAGCACGAGTACGATCATCTTG
ACGGTATTCTTTTTTTGGATCGCATTGACGAAAAGCGGAGGGATGATGCCCTTCGTCGGTATGCGGCGCTGCGCGGGACG
ATTCGTTAG

Upstream 100 bases:

>100_bases
GCGTTGCGGCGCAAGCTGATGAAGAAGGGGCGCAAGGTCAGCGACTCAAGGCTGTACTAGGCGGGTATGAGTTTCTCTTG
GGTAAGAGCGGGAAGAGGCG

Downstream 100 bases:

>100_bases
CCCATGGTGAGGGTCTTCTTCGCGGGAACTCCAGAGTGTGCCGTGCCTTCTTTGCGCCGGGTGGCGTGCGCTCATCGGGT
TGTGGGGGTTTTGACGAATC

Product: polypeptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MELKFLGEPCLTTVSEPVSEVDEQLRAFISGMFRVMRGAGGVGLAAPQVGRTVRVFVVDVEHHVRAFINPQITAASEEQS
SYEEGCLSIPHIYERVLRPRRVSVQYLDENGKRCAVDADGILARVIQHEYDHLDGILFLDRIDEKRRDDALRRYAALRGT
IR

Sequences:

>Translated_162_residues
MELKFLGEPCLTTVSEPVSEVDEQLRAFISGMFRVMRGAGGVGLAAPQVGRTVRVFVVDVEHHVRAFINPQITAASEEQS
SYEEGCLSIPHIYERVLRPRRVSVQYLDENGKRCAVDADGILARVIQHEYDHLDGILFLDRIDEKRRDDALRRYAALRGT
IR
>Mature_162_residues
MELKFLGEPCLTTVSEPVSEVDEQLRAFISGMFRVMRGAGGVGLAAPQVGRTVRVFVVDVEHHVRAFINPQITAASEEQS
SYEEGCLSIPHIYERVLRPRRVSVQYLDENGKRCAVDADGILARVIQHEYDHLDGILFLDRIDEKRRDDALRRYAALRGT
IR

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family

Homologues:

Organism=Homo sapiens, GI11641243, Length=162, Percent_Identity=30.2469135802469, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1789682, Length=133, Percent_Identity=42.1052631578947, Blast_Score=99, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24645728, Length=160, Percent_Identity=29.375, Blast_Score=77, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24645726, Length=160, Percent_Identity=28.125, Blast_Score=73, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DEF_TREPA (O83738)

Other databases:

- EMBL:   AE000520
- PIR:   H71284
- RefSeq:   NP_219194.1
- ProteinModelPortal:   O83738
- SMR:   O83738
- IntAct:   O83738
- MINT:   MINT-6481412
- GeneID:   2611341
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0757
- NMPDR:   fig|243276.1.peg.753
- TIGR:   TP_0757
- HOGENOM:   HBG665227
- OMA:   EFLAIVM
- ProtClustDB:   CLSK218851
- BioCyc:   TPAL243276:TP_0757-MONOMER
- BRENDA:   3.5.1.88
- GO:   GO:0006412
- HAMAP:   MF_00163
- InterPro:   IPR000181
- Gene3D:   G3DSA:3.90.45.10
- PANTHER:   PTHR10458
- PIRSF:   PIRSF004749
- PRINTS:   PR01576
- TIGRFAMs:   TIGR00079

Pfam domain/function: PF01327 Pep_deformylase; SSF56420 Fmet_deformylase

EC number: =3.5.1.88

Molecular weight: Translated: 18323; Mature: 18323

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: NA

Important sites: ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELKFLGEPCLTTVSEPVSEVDEQLRAFISGMFRVMRGAGGVGLAAPQVGRTVRVFVVDV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEH
EHHVRAFINPQITAASEEQSSYEEGCLSIPHIYERVLRPRRVSVQYLDENGKRCAVDADG
HHHHHHHCCCCCEECCCHHHHHHHCCCCCHHHHHHHCCCCCCEEEEECCCCCEEEECHHH
ILARVIQHEYDHLDGILFLDRIDEKRRDDALRRYAALRGTIR
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MELKFLGEPCLTTVSEPVSEVDEQLRAFISGMFRVMRGAGGVGLAAPQVGRTVRVFVVDV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEH
EHHVRAFINPQITAASEEQSSYEEGCLSIPHIYERVLRPRRVSVQYLDENGKRCAVDADG
HHHHHHHCCCCCEECCCHHHHHHHCCCCCHHHHHHHCCCCCCEEEEECCCCCEEEECHHH
ILARVIQHEYDHLDGILFLDRIDEKRRDDALRRYAALRGTIR
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876