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The map label for this gene is fmt

Identifier: 189025981

GI number: 189025981

Start: 821778

End: 822737

Strand: Reverse

Name: fmt

Synonym: TPASS_0756

Alternate gene names: 189025981

Gene position: 822737-821778 (Counterclockwise)

Preceding gene: 189025982

Following gene: 189025980

Centisome position: 72.2

GC content: 55.42

Gene sequence:

>960_bases
ATGGTGAGGGTCTTCTTCGCGGGAACTCCAGAGTGTGCCGTGCCTTCTTTGCGCCGGGTGGCGTGCGCTCATCGGGTTGT
GGGGGTTTTGACGAATCCTCCTGCTGCTGTCGGACGCTCGGGGAAGCTTGTCCATTCTGCGGTTGCAAGGGAATTTTTTC
GCTTAAAAGCGTCTGGGGTTCTTCCTGAGTCTGCGTCTCTTTTCGTTCCGGGGCGTTTGGATCGCGCCTTTTATGACGCG
GTGGAGGCTTTGCGTCCGGACGTGCTGGTGTGTTTTGCCTACGGTAAGATTTTTGGCCCTCGGTTCCTTGCGCTTTTCCC
GCGCGGTGCTATTAACGTTCATCCTTCGTTGTTGCCGCGTTGGCGAGGGAGTACACCGGTCCCTGCGGCGATTTTAGCGG
GGGACTGCGAGACGGGTGTGACGCTCCAGTACATTGGTGAGGAGATGGATGCAGGGGACATTCTTGCGCAGTCCCGTGTT
CAGCTCGACGGTACAGAGACTACTGGCGCGCTTCTTTCTCGTTTGTCTTTGGTGGCTGCCGATCTTGTGGATGATGTTTT
AGTCGGTGTTGAGCGGCACACGCTCGCCCCTGCAGCACAGGATCACTCGCAGGCAACATTTTGTGGTAAGCTCTGTAGGG
AGATGGGATTGGCGGACTGGAGTAATCCTGCAGTTGTGTTGGAGCGCAAGATTCGGGCCTTTACTCCCTGGCCGGGTCTA
TTCACCTATAAAGATGGGGAAAGGATAGCGATTTTGCAGGCGAGGTCGTGTGAGTCTTCGTTTGTTCCCCTCGCTCCTGT
GGGGACAGTGCTTGCTGCAGATAAAAATGGGGTGTTTGTCCAGACAGGCGATGGAGTTCTGTCCCTTTTACAGTTGCAGC
GCTCCGGGAAAAAACCTCTGTTTTGGAGAGATTTCCTCAATGGTTCCCCTCTCTTGCTGACAGGTAGGTTAGGGGTGTGA

Upstream 100 bases:

>100_bases
TCATCTTGACGGTATTCTTTTTTTGGATCGCATTGACGAAAAGCGGAGGGATGATGCCCTTCGTCGGTATGCGGCGCTGC
GCGGGACGATTCGTTAGCCC

Downstream 100 bases:

>100_bases
GTGATACACGCCAGGCGTGAGATTTCTACGCAACGCATGATGCGTACCCCAAGTGTGTCTTGTTACAGAGAAAGGGGAGG
TTGGTTTGTCCGAAGAAATT

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 319; Mature: 319

Protein sequence:

>319_residues
MVRVFFAGTPECAVPSLRRVACAHRVVGVLTNPPAAVGRSGKLVHSAVAREFFRLKASGVLPESASLFVPGRLDRAFYDA
VEALRPDVLVCFAYGKIFGPRFLALFPRGAINVHPSLLPRWRGSTPVPAAILAGDCETGVTLQYIGEEMDAGDILAQSRV
QLDGTETTGALLSRLSLVAADLVDDVLVGVERHTLAPAAQDHSQATFCGKLCREMGLADWSNPAVVLERKIRAFTPWPGL
FTYKDGERIAILQARSCESSFVPLAPVGTVLAADKNGVFVQTGDGVLSLLQLQRSGKKPLFWRDFLNGSPLLLTGRLGV

Sequences:

>Translated_319_residues
MVRVFFAGTPECAVPSLRRVACAHRVVGVLTNPPAAVGRSGKLVHSAVAREFFRLKASGVLPESASLFVPGRLDRAFYDA
VEALRPDVLVCFAYGKIFGPRFLALFPRGAINVHPSLLPRWRGSTPVPAAILAGDCETGVTLQYIGEEMDAGDILAQSRV
QLDGTETTGALLSRLSLVAADLVDDVLVGVERHTLAPAAQDHSQATFCGKLCREMGLADWSNPAVVLERKIRAFTPWPGL
FTYKDGERIAILQARSCESSFVPLAPVGTVLAADKNGVFVQTGDGVLSLLQLQRSGKKPLFWRDFLNGSPLLLTGRLGV
>Mature_319_residues
MVRVFFAGTPECAVPSLRRVACAHRVVGVLTNPPAAVGRSGKLVHSAVAREFFRLKASGVLPESASLFVPGRLDRAFYDA
VEALRPDVLVCFAYGKIFGPRFLALFPRGAINVHPSLLPRWRGSTPVPAAILAGDCETGVTLQYIGEEMDAGDILAQSRV
QLDGTETTGALLSRLSLVAADLVDDVLVGVERHTLAPAAQDHSQATFCGKLCREMGLADWSNPAVVLERKIRAFTPWPGL
FTYKDGERIAILQARSCESSFVPLAPVGTVLAADKNGVFVQTGDGVLSLLQLQRSGKKPLFWRDFLNGSPLLLTGRLGV

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI164663775, Length=235, Percent_Identity=29.3617021276596, Blast_Score=75, Evalue=8e-14,
Organism=Homo sapiens, GI238814322, Length=265, Percent_Identity=26.0377358490566, Blast_Score=65, Evalue=8e-11,
Organism=Escherichia coli, GI1789683, Length=311, Percent_Identity=38.2636655948553, Blast_Score=179, Evalue=2e-46,
Organism=Escherichia coli, GI1788589, Length=242, Percent_Identity=29.3388429752066, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1787483, Length=99, Percent_Identity=33.3333333333333, Blast_Score=71, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17567511, Length=108, Percent_Identity=32.4074074074074, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI45550868, Length=312, Percent_Identity=27.2435897435897, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI28571984, Length=226, Percent_Identity=30.5309734513274, Blast_Score=101, Evalue=8e-22,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_TREPA (O83737)

Other databases:

- EMBL:   AE000520
- PIR:   G71284
- RefSeq:   NP_219193.1
- ProteinModelPortal:   O83737
- SMR:   O83737
- IntAct:   O83737
- GeneID:   2611340
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0756
- NMPDR:   fig|243276.1.peg.752
- TIGR:   TP_0756
- HOGENOM:   HBG571560
- OMA:   QIRACTP
- ProtClustDB:   CLSK218850
- BioCyc:   TPAL243276:TP_0756-MONOMER
- BRENDA:   2.1.2.9
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 34261; Mature: 34261

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRVFFAGTPECAVPSLRRVACAHRVVGVLTNPPAAVGRSGKLVHSAVAREFFRLKASGV
CEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHCCC
LPESASLFVPGRLDRAFYDAVEALRPDVLVCFAYGKIFGPRFLALFPRGAINVHPSLLPR
CCCCCCEEECCCHHHHHHHHHHHHCCCEEEEEEHHHHHCCCEEEECCCCCCCCCCCCCCC
WRGSTPVPAAILAGDCETGVTLQYIGEEMDAGDILAQSRVQLDGTETTGALLSRLSLVAA
CCCCCCCCCEEEECCCCCCCEEEECCCCCCCHHHHHHCEEEECCCCHHHHHHHHHHHHHH
DLVDDVLVGVERHTLAPAAQDHSQATFCGKLCREMGLADWSNPAVVLERKIRAFTPWPGL
HHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHCCCCCCE
FTYKDGERIAILQARSCESSFVPLAPVGTVLAADKNGVFVQTGDGVLSLLQLQRSGKKPL
EEECCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCEEEEECCHHHHHHHHHHCCCCCE
FWRDFLNGSPLLLTGRLGV
EEHHHCCCCCEEEEECCCC
>Mature Secondary Structure
MVRVFFAGTPECAVPSLRRVACAHRVVGVLTNPPAAVGRSGKLVHSAVAREFFRLKASGV
CEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHCCCCCCHHHHHHHHHHHHHHHCCC
LPESASLFVPGRLDRAFYDAVEALRPDVLVCFAYGKIFGPRFLALFPRGAINVHPSLLPR
CCCCCCEEECCCHHHHHHHHHHHHCCCEEEEEEHHHHHCCCEEEECCCCCCCCCCCCCCC
WRGSTPVPAAILAGDCETGVTLQYIGEEMDAGDILAQSRVQLDGTETTGALLSRLSLVAA
CCCCCCCCCEEEECCCCCCCEEEECCCCCCCHHHHHHCEEEECCCCHHHHHHHHHHHHHH
DLVDDVLVGVERHTLAPAAQDHSQATFCGKLCREMGLADWSNPAVVLERKIRAFTPWPGL
HHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEHHHHHCCCCCCE
FTYKDGERIAILQARSCESSFVPLAPVGTVLAADKNGVFVQTGDGVLSLLQLQRSGKKPL
EEECCCCEEEEEEECCCCCCCCCCCCCCEEEEECCCCEEEEECCHHHHHHHHHHCCCCCE
FWRDFLNGSPLLLTGRLGV
EEHHHCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876