Click here to switch to the map view.

The map label for this gene is obgE

Identifier: 189025967

GI number: 189025967

Start: 808213

End: 809343

Strand: Reverse

Name: obgE

Synonym: TPASS_0742

Alternate gene names: 189025967

Gene position: 809343-808213 (Counterclockwise)

Preceding gene: 189025968

Following gene: 189025966

Centisome position: 71.03

GC content: 57.74

Gene sequence:

>1131_bases
ATGGCCAGTTTTGTTGATGAGGTGCTGATTCGTGTTTCCTCTGGTCGGGGTGGAAATGGCTGTGTGGCGTTTCGGCGGGA
AAAGTATGTCCCGCGCGGCGGCCCCGCGGGGGGCGATGGAGGGCGCGGCGGGGACGTTGTGTTCCAGGTACGGCGCAACA
TGCGCACGCTTGTGCACCTGAGGTATGGACGCGTGTTTCGTGCAAAGAATGGGCAGGATGGAGAGGGGGCACGCCGCTTT
GGTGCAAAGGGGCACGATTGTGTTATACCGCTGCCTCCGGGTTGTCTTTTAAGGGATGCGCAGACTCATGAGGTTTTGCA
CGATTTTGGTCATGCCCATGAAGGTTGCGTGACGCTCCTTTCGGGTGGAAGGGGTGGTTGGGGGAATTATCATTTCCGTG
GCCCAGTGCAGCAGGCTCCGCAACGCGCGCATTCTGGGCAGCCGGGGCAGGAACGTGTGGTGCACGTTGAACTGCGTATT
GTGGCAGACGTTGGCTTTGTGGGGCTCCCCAACGCGGGCAAATCTTCTTTGCTGAATTTTTTTACCCACGCGCGGTCGCG
CGTTGCCCCTTATCCTTTCACTACCCGGATTCCTTACTTGGGGGTGCTGCGTACGGGGGATGGGCGCGACGTGATCCTGG
CAGATGTCCCTGGGATTCTCGAACGCGCCTCGCAGGGTGTCGGCTTGGGGTTGCGCTTTCTCAAGCACTTGACCCGCTGT
GCGGGGCTTGCATTTCTCATTGATCTTGCAGATGAGCGTGCGCTGCATACATACGATTTGCTTTGCAAGGAATTGTACGC
TTTCTCCCCTGTCTTTGAGACAAAGGCGCGCGTGCTCGTAGGTACCAAGCTTGATTTGCCGAATGCGCGTGAGTGTTTGC
AGCAGCTTCGTGCACAGCACCCATCCACTGAGGTTTGTGGAGTCTCGGTGCACAATCGCTGGGGTTTAGATGAATTGCAG
GAGGCTTTTGTGCGTCTCTCTGACGCAGGTGCGGGCGCGTTGCGTTCCCCTGTGTGGCGGAACCAAGCTCCCAGTTTTAT
GTACGCTCAGCTTGAGGATCCGGTGTGTCAGGTGCGTGATGATTTTGGGGCAACGGTGAGCTTGAGCAGAAAACGAAAAG
TGCGCGGATGA

Upstream 100 bases:

>100_bases
CAGGTGCCTCCTGTGCTTTGTGTCTGTGGTGTAAGAAGGGTCAGGGGGTATTTGCGTGTCTGTGGGATCGTAGGGGGCGT
GCACAGGTTTTTGAGAGAGC

Downstream 100 bases:

>100_bases
AGTTAGCCCTGTTTGGCGGTTCGTACGATCCTGTTCATCTGGGCCACTTGCTCTTGGCTGATGCAGTACACCGGCACGCC
GGGTATGACCGCGTGCTGTT

Product: GTPase ObgE

Products: NA

Alternate protein names: GTP-binding protein obg

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MASFVDEVLIRVSSGRGGNGCVAFRREKYVPRGGPAGGDGGRGGDVVFQVRRNMRTLVHLRYGRVFRAKNGQDGEGARRF
GAKGHDCVIPLPPGCLLRDAQTHEVLHDFGHAHEGCVTLLSGGRGGWGNYHFRGPVQQAPQRAHSGQPGQERVVHVELRI
VADVGFVGLPNAGKSSLLNFFTHARSRVAPYPFTTRIPYLGVLRTGDGRDVILADVPGILERASQGVGLGLRFLKHLTRC
AGLAFLIDLADERALHTYDLLCKELYAFSPVFETKARVLVGTKLDLPNARECLQQLRAQHPSTEVCGVSVHNRWGLDELQ
EAFVRLSDAGAGALRSPVWRNQAPSFMYAQLEDPVCQVRDDFGATVSLSRKRKVRG

Sequences:

>Translated_376_residues
MASFVDEVLIRVSSGRGGNGCVAFRREKYVPRGGPAGGDGGRGGDVVFQVRRNMRTLVHLRYGRVFRAKNGQDGEGARRF
GAKGHDCVIPLPPGCLLRDAQTHEVLHDFGHAHEGCVTLLSGGRGGWGNYHFRGPVQQAPQRAHSGQPGQERVVHVELRI
VADVGFVGLPNAGKSSLLNFFTHARSRVAPYPFTTRIPYLGVLRTGDGRDVILADVPGILERASQGVGLGLRFLKHLTRC
AGLAFLIDLADERALHTYDLLCKELYAFSPVFETKARVLVGTKLDLPNARECLQQLRAQHPSTEVCGVSVHNRWGLDELQ
EAFVRLSDAGAGALRSPVWRNQAPSFMYAQLEDPVCQVRDDFGATVSLSRKRKVRG
>Mature_375_residues
ASFVDEVLIRVSSGRGGNGCVAFRREKYVPRGGPAGGDGGRGGDVVFQVRRNMRTLVHLRYGRVFRAKNGQDGEGARRFG
AKGHDCVIPLPPGCLLRDAQTHEVLHDFGHAHEGCVTLLSGGRGGWGNYHFRGPVQQAPQRAHSGQPGQERVVHVELRIV
ADVGFVGLPNAGKSSLLNFFTHARSRVAPYPFTTRIPYLGVLRTGDGRDVILADVPGILERASQGVGLGLRFLKHLTRCA
GLAFLIDLADERALHTYDLLCKELYAFSPVFETKARVLVGTKLDLPNARECLQQLRAQHPSTEVCGVSVHNRWGLDELQE
AFVRLSDAGAGALRSPVWRNQAPSFMYAQLEDPVCQVRDDFGATVSLSRKRKVRG

Specific function: An essential GTPase which binds GTP, GDP and possibly (p)ppGpp with moderate affinity, with high nucleotide exchange rates and a fairly low GTP hydrolysis rate. It may play a role in control of the cell cycle, stress response, ribosome biogenesis and in t

COG id: COG0536

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI24308117, Length=330, Percent_Identity=36.969696969697, Blast_Score=149, Evalue=6e-36,
Organism=Homo sapiens, GI111955139, Length=305, Percent_Identity=33.7704918032787, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI111955063, Length=151, Percent_Identity=38.4105960264901, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1789574, Length=299, Percent_Identity=42.1404682274247, Blast_Score=207, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17508313, Length=323, Percent_Identity=34.0557275541796, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17552324, Length=309, Percent_Identity=24.5954692556634, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6321962, Length=159, Percent_Identity=37.7358490566038, Blast_Score=116, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6319281, Length=105, Percent_Identity=38.0952380952381, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI20129375, Length=327, Percent_Identity=36.697247706422, Blast_Score=161, Evalue=9e-40,
Organism=Drosophila melanogaster, GI24585318, Length=307, Percent_Identity=31.5960912052117, Blast_Score=127, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OBG_TREPA (O83724)

Other databases:

- EMBL:   AE000520
- PIR:   C71286
- RefSeq:   NP_219179.1
- HSSP:   P44681
- ProteinModelPortal:   O83724
- SMR:   O83724
- GeneID:   2611092
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0742
- NMPDR:   fig|243276.1.peg.738
- TIGR:   TP_0742
- HOGENOM:   HBG716038
- OMA:   ATCEHAG
- ProtClustDB:   PRK12298
- BioCyc:   TPAL243276:TP_0742-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01454
- InterPro:   IPR014100
- InterPro:   IPR006073
- InterPro:   IPR006169
- InterPro:   IPR002917
- Gene3D:   G3DSA:2.70.210.12
- PIRSF:   PIRSF002401
- PRINTS:   PR00326
- TIGRFAMs:   TIGR02729

Pfam domain/function: PF01018 GTP1_OBG; PF01926 MMR_HSR1; SSF82051 GTP1_OBG_sub

EC number: NA

Molecular weight: Translated: 41146; Mature: 41014

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS00905 GTP1_OBG; PS00133 CARBOXYPEPT_ZN_2 ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASFVDEVLIRVSSGRGGNGCVAFRREKYVPRGGPAGGDGGRGGDVVFQVRRNMRTLVHL
CCHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RYGRVFRAKNGQDGEGARRFGAKGHDCVIPLPPGCLLRDAQTHEVLHDFGHAHEGCVTLL
HHCCEEECCCCCCCCHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCHHHEE
SGGRGGWGNYHFRGPVQQAPQRAHSGQPGQERVVHVELRIVADVGFVGLPNAGKSSLLNF
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHCEEEEEEEEEEECCEEECCCCCHHHHHHH
FTHARSRVAPYPFTTRIPYLGVLRTGDGRDVILADVPGILERASQGVGLGLRFLKHLTRC
HHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHH
AGLAFLIDLADERALHTYDLLCKELYAFSPVFETKARVLVGTKLDLPNARECLQQLRAQH
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCCCHHHHHHHHHHCC
PSTEVCGVSVHNRWGLDELQEAFVRLSDAGAGALRSPVWRNQAPSFMYAQLEDPVCQVRD
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEEEECCCCHHHHHH
DFGATVSLSRKRKVRG
CCCCEEEECCHHCCCC
>Mature Secondary Structure 
ASFVDEVLIRVSSGRGGNGCVAFRREKYVPRGGPAGGDGGRGGDVVFQVRRNMRTLVHL
CHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
RYGRVFRAKNGQDGEGARRFGAKGHDCVIPLPPGCLLRDAQTHEVLHDFGHAHEGCVTLL
HHCCEEECCCCCCCCHHHHCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCCCHHHEE
SGGRGGWGNYHFRGPVQQAPQRAHSGQPGQERVVHVELRIVADVGFVGLPNAGKSSLLNF
CCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHCEEEEEEEEEEECCEEECCCCCHHHHHHH
FTHARSRVAPYPFTTRIPYLGVLRTGDGRDVILADVPGILERASQGVGLGLRFLKHLTRC
HHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEEECCCHHHHHHCCCCCHHHHHHHHHHHH
AGLAFLIDLADERALHTYDLLCKELYAFSPVFETKARVLVGTKLDLPNARECLQQLRAQH
HHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCCCHHHHHHHHHHCC
PSTEVCGVSVHNRWGLDELQEAFVRLSDAGAGALRSPVWRNQAPSFMYAQLEDPVCQVRD
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEEEEECCCCHHHHHH
DFGATVSLSRKRKVRG
CCCCEEEECCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9665876