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The map label for this gene is gltA [H]

Identifier: 189025960

GI number: 189025960

Start: 801315

End: 802871

Strand: Reverse

Name: gltA [H]

Synonym: TPASS_0735

Alternate gene names: 189025960

Gene position: 802871-801315 (Counterclockwise)

Preceding gene: 189025961

Following gene: 189025959

Centisome position: 70.46

GC content: 55.11

Gene sequence:

>1557_bases
ATGACATCTGTACAAAATGTCGTAAGCGATGCCAAGACGTGTGACGTACCGGTTGAGTCAGAACCAGAAGCGCGTACGTA
TCGGCCGCACGCACTGCTCCAGGAAGAAGCGTGCCAGCGCCTGGCGCAGCTCCAGGGTAAAGAGCTGAAGATGAAGGATC
GCACGCAGATCCCTTTGCAGCGTATGCGTATGCTGCCGCCAAAAAAGCGGTCGCTCCTCATGCAGGAGGCGGCGCTGGGT
TTTACAGAGCAGCAGGCACTGGTTGAGTCTCAGCGTTGTTTGAATTGTAAAACCAAGCCGTGCGTAAAGGGTTGTCCGGT
AGGAGTGCCGATTCCTGAGTTTATTGCGTGTGTCCAAAGGGGAGCCTTTAAGGAAGCAGTTGATATCATTAAGACGACGA
GTTTACTCCCTGCAATTTGCGGTCGTGTGTGCCCGCATGAGCGTCAGTGTCAATTGCAGTGTACCGTGGGAAAGATGTTT
AAGGATGTGTCCAAGGCGGTGTCGATTGGTGCGCTTGAGCGTTTTGTCGCTGACTGGGAGCGGCAGCATGGTCAAATTAC
AGTTCCGTACTGTGCGCCGTCTACGCACAAGAAAGTGGCCGTCATCGGGTCAGGGCCTGCAGGTCTTGCAGTTGCCTCAG
ATACGGCGCGTGCAGGCCATTCGGTAACGGTGTTCGAAGCGCTCCACAAACCGGGTGGAGTGGTGACCTATGGTATTCCT
GAGTTTCGCCTGCCGAAAGAAGTGGTAGTGACGGAAATTGAAACACTCAAGAAAATGGGGGTGACGTTCCGGATGAACTT
TTTGGTGGGCAGAACAGCAACGCTGGAACAGCTGTTCTCGCAGTACGGTTTTGATGCAGTTTTTATCGGGACCGGTGCGG
GATTGCCGCGCTTTATGAATATTGAGGGTGAGGAATTGTGCGGGGTGTTTGCAGCAAACGACTACCTAACGCGTGCGACG
CTGATGAAGGCGTATGACACTGCGCATGCGGACACGCCGGTGTATGCGGCAAAGTCAGTGGTGGTAGTCGGTGGAGGGAA
TGTGGCGGTAGATTCCTCGCGTACGGCGCTCCGTTTAGGGGCTGAGCAGGTGCACTGTCTGTACAGAAAAACGCGTGCGG
ATATGACCGCCTGTGTGGAGGAAATTGCACAGGCAGAAGATGAGGGGGTGACGTTTCACTTTTTGTGTCAGACCACCCGC
ATTTTGGGGGATGAAGAGGGGAACGTGCGCGCGGTAGTGTTCCGCGATTGTCAGGAACAGATTGATGCAGGAGAACGTGT
GTTTCTGCCCTGCGGCGATGCTGAGTGTAAACTTGCTGCAGACGCGGTGATCGTTGCCGTTGGGAATGGTTCAAATCCGC
TCATGGCAAAGACAACGCGCAGTCTTGCAGTGAGCGAGCGGGGTACTATCGTGGTGGACGAGGATCAACGGACGAGCATT
CCGGGAGTGTGGGCTGGGGGAGACATTGTCTTAGGAGCGGCAACGGTTATTCGTGCGATGGGGCAGGGGCGGCGTGCAGC
GGCGTCTATAAACGCGTACCTTGCGCAAAAAGCGTAA

Upstream 100 bases:

>100_bases
TTGATTTTGAGAGCATGATGCGGAGAATGAGGGCATTTCAGGCAAGGGAAGCGCATGATGTCCACTGCTGTAAGATGAAG
CACCACGCGGGGAGGTAGGT

Downstream 100 bases:

>100_bases
CGGGTGCTGCATGCGCTGAGCGTACACGCGCGAGGTTACCGAAACGTGTGGGTCTAGGCCCCACGAAGGGAGGGAGTGAC
GATGAGTATTCACCTTGGAG

Product: oxidoreductase

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 518; Mature: 517

Protein sequence:

>518_residues
MTSVQNVVSDAKTCDVPVESEPEARTYRPHALLQEEACQRLAQLQGKELKMKDRTQIPLQRMRMLPPKKRSLLMQEAALG
FTEQQALVESQRCLNCKTKPCVKGCPVGVPIPEFIACVQRGAFKEAVDIIKTTSLLPAICGRVCPHERQCQLQCTVGKMF
KDVSKAVSIGALERFVADWERQHGQITVPYCAPSTHKKVAVIGSGPAGLAVASDTARAGHSVTVFEALHKPGGVVTYGIP
EFRLPKEVVVTEIETLKKMGVTFRMNFLVGRTATLEQLFSQYGFDAVFIGTGAGLPRFMNIEGEELCGVFAANDYLTRAT
LMKAYDTAHADTPVYAAKSVVVVGGGNVAVDSSRTALRLGAEQVHCLYRKTRADMTACVEEIAQAEDEGVTFHFLCQTTR
ILGDEEGNVRAVVFRDCQEQIDAGERVFLPCGDAECKLAADAVIVAVGNGSNPLMAKTTRSLAVSERGTIVVDEDQRTSI
PGVWAGGDIVLGAATVIRAMGQGRRAAASINAYLAQKA

Sequences:

>Translated_518_residues
MTSVQNVVSDAKTCDVPVESEPEARTYRPHALLQEEACQRLAQLQGKELKMKDRTQIPLQRMRMLPPKKRSLLMQEAALG
FTEQQALVESQRCLNCKTKPCVKGCPVGVPIPEFIACVQRGAFKEAVDIIKTTSLLPAICGRVCPHERQCQLQCTVGKMF
KDVSKAVSIGALERFVADWERQHGQITVPYCAPSTHKKVAVIGSGPAGLAVASDTARAGHSVTVFEALHKPGGVVTYGIP
EFRLPKEVVVTEIETLKKMGVTFRMNFLVGRTATLEQLFSQYGFDAVFIGTGAGLPRFMNIEGEELCGVFAANDYLTRAT
LMKAYDTAHADTPVYAAKSVVVVGGGNVAVDSSRTALRLGAEQVHCLYRKTRADMTACVEEIAQAEDEGVTFHFLCQTTR
ILGDEEGNVRAVVFRDCQEQIDAGERVFLPCGDAECKLAADAVIVAVGNGSNPLMAKTTRSLAVSERGTIVVDEDQRTSI
PGVWAGGDIVLGAATVIRAMGQGRRAAASINAYLAQKA
>Mature_517_residues
TSVQNVVSDAKTCDVPVESEPEARTYRPHALLQEEACQRLAQLQGKELKMKDRTQIPLQRMRMLPPKKRSLLMQEAALGF
TEQQALVESQRCLNCKTKPCVKGCPVGVPIPEFIACVQRGAFKEAVDIIKTTSLLPAICGRVCPHERQCQLQCTVGKMFK
DVSKAVSIGALERFVADWERQHGQITVPYCAPSTHKKVAVIGSGPAGLAVASDTARAGHSVTVFEALHKPGGVVTYGIPE
FRLPKEVVVTEIETLKKMGVTFRMNFLVGRTATLEQLFSQYGFDAVFIGTGAGLPRFMNIEGEELCGVFAANDYLTRATL
MKAYDTAHADTPVYAAKSVVVVGGGNVAVDSSRTALRLGAEQVHCLYRKTRADMTACVEEIAQAEDEGVTFHFLCQTTRI
LGDEEGNVRAVVFRDCQEQIDAGERVFLPCGDAECKLAADAVIVAVGNGSNPLMAKTTRSLAVSERGTIVVDEDQRTSIP
GVWAGGDIVLGAATVIRAMGQGRRAAASINAYLAQKA

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=453, Percent_Identity=30.242825607064, Blast_Score=169, Evalue=6e-42,
Organism=Escherichia coli, GI1789606, Length=470, Percent_Identity=36.8085106382979, Blast_Score=255, Evalue=6e-69,
Organism=Escherichia coli, GI1788811, Length=464, Percent_Identity=35.7758620689655, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI87082180, Length=461, Percent_Identity=37.9609544468547, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1788468, Length=444, Percent_Identity=34.009009009009, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1789243, Length=486, Percent_Identity=29.8353909465021, Blast_Score=169, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17570289, Length=278, Percent_Identity=33.0935251798561, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI71984108, Length=457, Percent_Identity=29.5404814004376, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17543860, Length=161, Percent_Identity=31.055900621118, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=477, Percent_Identity=29.559748427673, Blast_Score=176, Evalue=6e-45,
Organism=Drosophila melanogaster, GI24665547, Length=514, Percent_Identity=28.2101167315175, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI24665543, Length=514, Percent_Identity=28.2101167315175, Blast_Score=179, Evalue=4e-45,
Organism=Drosophila melanogaster, GI28574881, Length=514, Percent_Identity=28.2101167315175, Blast_Score=179, Evalue=6e-45,
Organism=Drosophila melanogaster, GI24665539, Length=514, Percent_Identity=28.2101167315175, Blast_Score=179, Evalue=6e-45,
Organism=Drosophila melanogaster, GI24640763, Length=454, Percent_Identity=29.7356828193833, Blast_Score=147, Evalue=2e-35,
Organism=Drosophila melanogaster, GI18858217, Length=454, Percent_Identity=29.7356828193833, Blast_Score=147, Evalue=2e-35,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 55951; Mature: 55820

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVQNVVSDAKTCDVPVESEPEARTYRPHALLQEEACQRLAQLQGKELKMKDRTQIPLQ
CCCHHHHHCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
RMRMLPPKKRSLLMQEAALGFTEQQALVESQRCLNCKTKPCVKGCPVGVPIPEFIACVQR
HHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHC
GAFKEAVDIIKTTSLLPAICGRVCPHERQCQLQCTVGKMFKDVSKAVSIGALERFVADWE
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
RQHGQITVPYCAPSTHKKVAVIGSGPAGLAVASDTARAGHSVTVFEALHKPGGVVTYGIP
HCCCCEEEEECCCCCCCEEEEEECCCCCEEEECCCCCCCCCCHHHHHHCCCCCEEEECCC
EFRLPKEVVVTEIETLKKMGVTFRMNFLVGRTATLEQLFSQYGFDAVFIGTGAGLPRFMN
CCCCCHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHCCCEEEEECCCCCCEEEC
IEGEELCGVFAANDYLTRATLMKAYDTAHADTPVYAAKSVVVVGGGNVAVDSSRTALRLG
CCCHHHHEEEECCCHHHHHHHHHHHHHCCCCCCCEECCEEEEEECCCEEECCCCHHHHHC
AEQVHCLYRKTRADMTACVEEIAQAEDEGVTFHFLCQTTRILGDEEGNVRAVVFRDCQEQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHCCCCCCCEEEEEEHHHHHH
IDAGERVFLPCGDAECKLAADAVIVAVGNGSNPLMAKTTRSLAVSERGTIVVDEDQRTSI
HCCCCEEEEECCCCCCEEECCEEEEEECCCCCCEEEHHHHHHEECCCCCEEECCCCCCCC
PGVWAGGDIVLGAATVIRAMGQGRRAAASINAYLAQKA
CCEECCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TSVQNVVSDAKTCDVPVESEPEARTYRPHALLQEEACQRLAQLQGKELKMKDRTQIPLQ
CCHHHHHCCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHH
RMRMLPPKKRSLLMQEAALGFTEQQALVESQRCLNCKTKPCVKGCPVGVPIPEFIACVQR
HHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHC
GAFKEAVDIIKTTSLLPAICGRVCPHERQCQLQCTVGKMFKDVSKAVSIGALERFVADWE
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHH
RQHGQITVPYCAPSTHKKVAVIGSGPAGLAVASDTARAGHSVTVFEALHKPGGVVTYGIP
HCCCCEEEEECCCCCCCEEEEEECCCCCEEEECCCCCCCCCCHHHHHHCCCCCEEEECCC
EFRLPKEVVVTEIETLKKMGVTFRMNFLVGRTATLEQLFSQYGFDAVFIGTGAGLPRFMN
CCCCCHHHHHHHHHHHHHCCCEEEEEEEECCHHHHHHHHHHHCCCEEEEECCCCCCEEEC
IEGEELCGVFAANDYLTRATLMKAYDTAHADTPVYAAKSVVVVGGGNVAVDSSRTALRLG
CCCHHHHEEEECCCHHHHHHHHHHHHHCCCCCCCEECCEEEEEECCCEEECCCCHHHHHC
AEQVHCLYRKTRADMTACVEEIAQAEDEGVTFHFLCQTTRILGDEEGNVRAVVFRDCQEQ
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHHHHCCCCCCCEEEEEEHHHHHH
IDAGERVFLPCGDAECKLAADAVIVAVGNGSNPLMAKTTRSLAVSERGTIVVDEDQRTSI
HCCCCEEEEECCCCCCEEECCEEEEEECCCCCCEEEHHHHHHEECCCCCEEECCCCCCCC
PGVWAGGDIVLGAATVIRAMGQGRRAAASINAYLAQKA
CCEECCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]