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The map label for this gene is folD

Identifier: 189025957

GI number: 189025957

Start: 798797

End: 799744

Strand: Reverse

Name: folD

Synonym: TPASS_0732

Alternate gene names: 189025957

Gene position: 799744-798797 (Counterclockwise)

Preceding gene: 189025959

Following gene: 189025954

Centisome position: 70.19

GC content: 62.03

Gene sequence:

>948_bases
GTGGACGCACGCCTCATCGATGGAAAACAAGCCGCACACGAGTGTACGGCGCGTCTTGCAACGCGTGTGCAGGCGCTGCG
TGCGGCGGTAGGCACGGCGCCCTTTCTTGCCGCAGTGCTGGTGGGGGATGACCCGGCTTCTTGTACCTACGTTGCAGCAA
AGCAGCGTGCCCTGGCTCGCGCCCACCTGCGGGGGGAAACGCACCGGCTGCCTGCTCATGCCTCTCACGCACAGGTGTTA
GAACTTATTGCTCGTCTCAATGAGGACGCGCGGGTGCACGGCATTTTGATCCAATTGCCGTTACCTGCGCATCTTGACGC
AGCGAGGGTGTGCCGCGCAGTGGCGCCAGAAAAAGATGTCGACGGCTTTCATCCGCTTAACTGTGGTGCGCTCTTCCTTG
CGCAGCCTGGGTTTGTTCCCTGCACCCCTGCGGGGATTGTGCACTTGCTCCGGCGTGCGCAGGTGCCGCTTGCGGGGGCA
CGCGTGGTAATTGTCGGCCGTTCTGCCATCGTTGGCCGTCCGCTTGCAGTGCTCCTTGCCTCTCCCGGCTGTGATGCAAC
CGTGACGCTCTGTCACAGTCACACGCGCGGTTTGGCGGATATCTGTGTGCAGGCAGATATCCTCGTTGCAGCACTGGGAA
AGGCGCGCTTCATTGGCGCGCCGTTTGTGCGCACCGGCGCCGTGGTTATCGATGTGGGTATTCATCACGTGCCAGATGCA
ACTGCACCGCGCGGGAGAAGACTGTGTGGTGATGTGGATTTTGACGCAGTGGCGCACAAGGTGCAGGCGATCACGCCTGT
TCCGGGGGGAGTAGGACCGATGACTATCGCGATGCTGCTGCACAACACGCTCTGCGCTGCAGAGTATGCAGCCGGGATGA
TACCCCCCTTTAGAGCGGCGCTCTATGCCGACCTAGATGGACGCGCTGCAGGCGACGTACCGCACTGA

Upstream 100 bases:

>100_bases
CTCATACGACGCCGAGCATACGGTGTTTCTTGGGCTTCCGTCTAGTGTCCGTGCGTGTTCGGTCCTTGCGCTGTTGCGTG
CGCGTGTCTATACTGGGCGC

Downstream 100 bases:

>100_bases
GTTACAGGGAGGAAAGCGGATGCGCCCGCAAAAAAAGAAAGAGGGCTGCGCCATGAGCGCGTGGTCAAAGGGTGCGCGGC
CCATGCGTTCGTACACGTCG

Product: methylenetetrahydrofolate dehydrogenase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MDARLIDGKQAAHECTARLATRVQALRAAVGTAPFLAAVLVGDDPASCTYVAAKQRALARAHLRGETHRLPAHASHAQVL
ELIARLNEDARVHGILIQLPLPAHLDAARVCRAVAPEKDVDGFHPLNCGALFLAQPGFVPCTPAGIVHLLRRAQVPLAGA
RVVIVGRSAIVGRPLAVLLASPGCDATVTLCHSHTRGLADICVQADILVAALGKARFIGAPFVRTGAVVIDVGIHHVPDA
TAPRGRRLCGDVDFDAVAHKVQAITPVPGGVGPMTIAMLLHNTLCAAEYAAGMIPPFRAALYADLDGRAAGDVPH

Sequences:

>Translated_315_residues
MDARLIDGKQAAHECTARLATRVQALRAAVGTAPFLAAVLVGDDPASCTYVAAKQRALARAHLRGETHRLPAHASHAQVL
ELIARLNEDARVHGILIQLPLPAHLDAARVCRAVAPEKDVDGFHPLNCGALFLAQPGFVPCTPAGIVHLLRRAQVPLAGA
RVVIVGRSAIVGRPLAVLLASPGCDATVTLCHSHTRGLADICVQADILVAALGKARFIGAPFVRTGAVVIDVGIHHVPDA
TAPRGRRLCGDVDFDAVAHKVQAITPVPGGVGPMTIAMLLHNTLCAAEYAAGMIPPFRAALYADLDGRAAGDVPH
>Mature_315_residues
MDARLIDGKQAAHECTARLATRVQALRAAVGTAPFLAAVLVGDDPASCTYVAAKQRALARAHLRGETHRLPAHASHAQVL
ELIARLNEDARVHGILIQLPLPAHLDAARVCRAVAPEKDVDGFHPLNCGALFLAQPGFVPCTPAGIVHLLRRAQVPLAGA
RVVIVGRSAIVGRPLAVLLASPGCDATVTLCHSHTRGLADICVQADILVAALGKARFIGAPFVRTGAVVIDVGIHHVPDA
TAPRGRRLCGDVDFDAVAHKVQAITPVPGGVGPMTIAMLLHNTLCAAEYAAGMIPPFRAALYADLDGRAAGDVPH

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI94721354, Length=295, Percent_Identity=43.728813559322, Blast_Score=228, Evalue=4e-60,
Organism=Homo sapiens, GI222136639, Length=310, Percent_Identity=39.3548387096774, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI222418558, Length=295, Percent_Identity=43.3898305084746, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI36796743, Length=225, Percent_Identity=28.8888888888889, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1786741, Length=290, Percent_Identity=45.8620689655172, Blast_Score=238, Evalue=5e-64,
Organism=Caenorhabditis elegans, GI17568735, Length=260, Percent_Identity=39.6153846153846, Blast_Score=168, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=44.9477351916376, Blast_Score=247, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6321643, Length=299, Percent_Identity=43.4782608695652, Blast_Score=231, Evalue=1e-61,
Organism=Saccharomyces cerevisiae, GI6322933, Length=324, Percent_Identity=24.0740740740741, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17136816, Length=293, Percent_Identity=46.4163822525597, Blast_Score=244, Evalue=6e-65,
Organism=Drosophila melanogaster, GI17136818, Length=293, Percent_Identity=46.4163822525597, Blast_Score=244, Evalue=7e-65,
Organism=Drosophila melanogaster, GI62472483, Length=289, Percent_Identity=43.2525951557093, Blast_Score=220, Evalue=8e-58,
Organism=Drosophila melanogaster, GI45551871, Length=289, Percent_Identity=43.2525951557093, Blast_Score=220, Evalue=8e-58,
Organism=Drosophila melanogaster, GI24645718, Length=289, Percent_Identity=43.2525951557093, Blast_Score=220, Evalue=1e-57,
Organism=Drosophila melanogaster, GI17137370, Length=289, Percent_Identity=43.2525951557093, Blast_Score=220, Evalue=1e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_TREPA (O83714)

Other databases:

- EMBL:   AE000520
- PIR:   A71288
- RefSeq:   NP_219169.1
- HSSP:   P11586
- ProteinModelPortal:   O83714
- SMR:   O83714
- GeneID:   2611053
- GenomeReviews:   AE000520_GR
- KEGG:   tpa:TP0732
- NMPDR:   fig|243276.1.peg.728
- TIGR:   TP_0732
- HOGENOM:   HBG328751
- OMA:   TYVRNKQ
- ProtClustDB:   CLSK2300284
- BioCyc:   TPAL243276:TP_0732-MONOMER
- BRENDA:   1.5.1.5
- BRENDA:   3.5.4.9
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 32820; Mature: 32820

Theoretical pI: Translated: 8.44; Mature: 8.44

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
3.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDARLIDGKQAAHECTARLATRVQALRAAVGTAPFLAAVLVGDDPASCTYVAAKQRALAR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEECCCCCCCHHHHHHHHHHHH
AHLRGETHRLPAHASHAQVLELIARLNEDARVHGILIQLPLPAHLDAARVCRAVAPEKDV
HHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHHHCCCCCC
DGFHPLNCGALFLAQPGFVPCTPAGIVHLLRRAQVPLAGARVVIVGRSAIVGRPLAVLLA
CCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCHHHCCCCEEEEE
SPGCDATVTLCHSHTRGLADICVQADILVAALGKARFIGAPFVRTGAVVIDVGIHHVPDA
CCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHCCHHCCCCCHHHCCEEEEEECCCCCCCC
TAPRGRRLCGDVDFDAVAHKVQAITPVPGGVGPMTIAMLLHNTLCAAEYAAGMIPPFRAA
CCCCCCCCCCCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LYADLDGRAAGDVPH
HEECCCCCCCCCCCC
>Mature Secondary Structure
MDARLIDGKQAAHECTARLATRVQALRAAVGTAPFLAAVLVGDDPASCTYVAAKQRALAR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEECCCCCCCHHHHHHHHHHHH
AHLRGETHRLPAHASHAQVLELIARLNEDARVHGILIQLPLPAHLDAARVCRAVAPEKDV
HHHCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHHHCCCCCC
DGFHPLNCGALFLAQPGFVPCTPAGIVHLLRRAQVPLAGARVVIVGRSAIVGRPLAVLLA
CCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCHHHCCCCEEEEE
SPGCDATVTLCHSHTRGLADICVQADILVAALGKARFIGAPFVRTGAVVIDVGIHHVPDA
CCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHHCCHHCCCCCHHHCCEEEEEECCCCCCCC
TAPRGRRLCGDVDFDAVAHKVQAITPVPGGVGPMTIAMLLHNTLCAAEYAAGMIPPFRAA
CCCCCCCCCCCCCHHHHHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
LYADLDGRAAGDVPH
HEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876