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The map label for this gene is thrS

Identifier: 188586439

GI number: 188586439

Start: 1897076

End: 1898986

Strand: Reverse

Name: thrS

Synonym: Nther_1824

Alternate gene names: 188586439

Gene position: 1898986-1897076 (Counterclockwise)

Preceding gene: 188586440

Following gene: 188586438

Centisome position: 59.99

GC content: 38.51

Gene sequence:

>1911_bases
ATGCTTAAAATCACCCTAAAAGACGGTTCGGTTAAAGAAATTGAAAAGGGTAGTAATGTACTGGACCTGGCCAAGTCGAT
TAGTAATCGTCTGGCTAAAGAAGCTGTTGGTGCAAAGGTAGACGGAAAGACTGTGGGCTTGAATAAAGAGCTAAATGAGA
ACTGTGAAGTTGAAATTTTAAGCTTTGAAGATGATGAAGGCTGTGATATTTATCGTCACACTTCGTCACACATTCTGGCT
CATGCAGTTAAACGCTTGTATGATAATGTTAAACTAGGTATTGGTCCTCCAATCAAAGATGGTTTTTACTATGATTTTGA
TTTGGAACATTCATTGTCAGATAAAGATCTGGAAGATATCAAAAAAGAGATGAAGAAAATAATCAAAGAGGATTTGCCCA
TTGAAAGAAAAGTATACAAAAGAGATGAAGCAATTGAAATGTTCAAGGAAATGGGTGAAGATTACAAGATTGAATTGATC
CAAGACCTTGGTGAAGATGAAGAAATTTGTTGCTATCAACAGGGAGACTTTATAGATCTATGCCAAGGACCTCACTTGCC
ATCAACAGGAAAGATAAAAGATACTGCTCTAGACTTGTTAAAAGTAGCAGGAGCCTATTGGAGAGGCGATGAAAACAGGG
CTATGCTGCAGAGGATATATGGTACTTCTTTTATAAAAAAATCTCAGCTTGAAGAATACATTCAAAGATTAGAAGAGGCC
AAGAAACGAGAACATAGAAAACTTGGAAAAGAACTGGATCTTTTCAGCTTTCATGAGGAAGCGCCTGGTTCTCCTTTCTT
CCATAACAATGGTATGATAGTTATCAATGAATTGATTGATTTTTGGAGACGGGAGCATCGTAAAGCGGGATATCAAGAAA
TTAGTACACCTATAATTTTGAACAGGGGACTGTGGGAACGCTCAGGTCACTGGGATCACTTTCAAGAAAATATGTACTTT
ACTGAAATCGATGATGAAGATTTTGCTGTGAAGCCTATGAATTGTCCTGGTGCCATGTTAGTTTACAATAATAATATGTA
CAGCTACCGTGATTTTCCCTTGCGCGTAGCAGAGCTTGGTAAAGTACATCGGCATGAACTGTCAGGGGCTCTTCATGGAT
TATTAAGAGCTCGAAGCTTTACCCAAGATGATGCCCATATATTTATGCTCCCTTCCCAAGTGGAAGAGGAACTGGAAAAT
GTCATTAATTTGGTAGATAGATTTTACAGTGTTTTTGGCTTTAATTATTATGTTGAGTTATCAACTAAACCGGAAAAAGC
TATGGGCTCAGATGAAATTTGGGATCGAGCTATCAATGCTTTGAAATCCGTACTAGAAAAACGTGGTATTAAATACATTG
TCAACGAAGGTGATGGAGCGTTTTACGGACCCAAAATTGACTTCCAATTGGAGGATGCCATCGGAAGGTCATGGCAGTGT
AGTACCATTCAACTTGATTTCTTGTTACCTGAACGCTTTGATTTGACCTATATAGGAGAAGATGGGCAGAAACATCGGCC
AGTTATGATACACAGAGTTATTTACGGAGCAATTGAACGATTTTTCGCTCTGTTAATAGAGCACTATGAAGGTAAATTCC
CACTTTGGCTAGCTCCAAGGCAGGCAGTAATTATTCCCATTGCCGATAGGCATATGGAATATGGAAAGGATATTACCAAA
CAGTTAGAATCTGAAGGTATCCGAGCTGATTTGGACCAACGCAATGAAAAAATTGGTTACAAAATTAGAGATGCTCAGCT
TAAAAAAGTTCCCTATATGTTAATTGTGGGTGACGACGAAGTGGAGAATAAAACTGTTTCCGTAAGGACCAGAGAAGATG
GCGACATTGGCTCAAAAAGCGTTGCCGAGCTAGTGGATGAAATAAAAGTTGAAATTGACGATATGGACTAA

Upstream 100 bases:

>100_bases
GGGTGGAACCGCGAGATACTCTCGTCCCTTGTATAGAGGATGGGAGTTTTTTTAGTTTTAAAAGTTATACCTTGAAAATA
AACGAGAGGGAGGTCTAAGA

Downstream 100 bases:

>100_bases
ATTGGCTGATTTGATATGAAATATAGAGCATAGATAGTATAAAAAGCATCGATAAATAGCAAATAAAGATTATGTATTAT
AATTGACTTTTATCCCTGTA

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 636; Mature: 636

Protein sequence:

>636_residues
MLKITLKDGSVKEIEKGSNVLDLAKSISNRLAKEAVGAKVDGKTVGLNKELNENCEVEILSFEDDEGCDIYRHTSSHILA
HAVKRLYDNVKLGIGPPIKDGFYYDFDLEHSLSDKDLEDIKKEMKKIIKEDLPIERKVYKRDEAIEMFKEMGEDYKIELI
QDLGEDEEICCYQQGDFIDLCQGPHLPSTGKIKDTALDLLKVAGAYWRGDENRAMLQRIYGTSFIKKSQLEEYIQRLEEA
KKREHRKLGKELDLFSFHEEAPGSPFFHNNGMIVINELIDFWRREHRKAGYQEISTPIILNRGLWERSGHWDHFQENMYF
TEIDDEDFAVKPMNCPGAMLVYNNNMYSYRDFPLRVAELGKVHRHELSGALHGLLRARSFTQDDAHIFMLPSQVEEELEN
VINLVDRFYSVFGFNYYVELSTKPEKAMGSDEIWDRAINALKSVLEKRGIKYIVNEGDGAFYGPKIDFQLEDAIGRSWQC
STIQLDFLLPERFDLTYIGEDGQKHRPVMIHRVIYGAIERFFALLIEHYEGKFPLWLAPRQAVIIPIADRHMEYGKDITK
QLESEGIRADLDQRNEKIGYKIRDAQLKKVPYMLIVGDDEVENKTVSVRTREDGDIGSKSVAELVDEIKVEIDDMD

Sequences:

>Translated_636_residues
MLKITLKDGSVKEIEKGSNVLDLAKSISNRLAKEAVGAKVDGKTVGLNKELNENCEVEILSFEDDEGCDIYRHTSSHILA
HAVKRLYDNVKLGIGPPIKDGFYYDFDLEHSLSDKDLEDIKKEMKKIIKEDLPIERKVYKRDEAIEMFKEMGEDYKIELI
QDLGEDEEICCYQQGDFIDLCQGPHLPSTGKIKDTALDLLKVAGAYWRGDENRAMLQRIYGTSFIKKSQLEEYIQRLEEA
KKREHRKLGKELDLFSFHEEAPGSPFFHNNGMIVINELIDFWRREHRKAGYQEISTPIILNRGLWERSGHWDHFQENMYF
TEIDDEDFAVKPMNCPGAMLVYNNNMYSYRDFPLRVAELGKVHRHELSGALHGLLRARSFTQDDAHIFMLPSQVEEELEN
VINLVDRFYSVFGFNYYVELSTKPEKAMGSDEIWDRAINALKSVLEKRGIKYIVNEGDGAFYGPKIDFQLEDAIGRSWQC
STIQLDFLLPERFDLTYIGEDGQKHRPVMIHRVIYGAIERFFALLIEHYEGKFPLWLAPRQAVIIPIADRHMEYGKDITK
QLESEGIRADLDQRNEKIGYKIRDAQLKKVPYMLIVGDDEVENKTVSVRTREDGDIGSKSVAELVDEIKVEIDDMD
>Mature_636_residues
MLKITLKDGSVKEIEKGSNVLDLAKSISNRLAKEAVGAKVDGKTVGLNKELNENCEVEILSFEDDEGCDIYRHTSSHILA
HAVKRLYDNVKLGIGPPIKDGFYYDFDLEHSLSDKDLEDIKKEMKKIIKEDLPIERKVYKRDEAIEMFKEMGEDYKIELI
QDLGEDEEICCYQQGDFIDLCQGPHLPSTGKIKDTALDLLKVAGAYWRGDENRAMLQRIYGTSFIKKSQLEEYIQRLEEA
KKREHRKLGKELDLFSFHEEAPGSPFFHNNGMIVINELIDFWRREHRKAGYQEISTPIILNRGLWERSGHWDHFQENMYF
TEIDDEDFAVKPMNCPGAMLVYNNNMYSYRDFPLRVAELGKVHRHELSGALHGLLRARSFTQDDAHIFMLPSQVEEELEN
VINLVDRFYSVFGFNYYVELSTKPEKAMGSDEIWDRAINALKSVLEKRGIKYIVNEGDGAFYGPKIDFQLEDAIGRSWQC
STIQLDFLLPERFDLTYIGEDGQKHRPVMIHRVIYGAIERFFALLIEHYEGKFPLWLAPRQAVIIPIADRHMEYGKDITK
QLESEGIRADLDQRNEKIGYKIRDAQLKKVPYMLIVGDDEVENKTVSVRTREDGDIGSKSVAELVDEIKVEIDDMD

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=632, Percent_Identity=43.9873417721519, Blast_Score=545, Evalue=1e-155,
Organism=Homo sapiens, GI38570111, Length=633, Percent_Identity=41.7061611374408, Blast_Score=512, Evalue=1e-145,
Organism=Homo sapiens, GI20070344, Length=654, Percent_Identity=37.4617737003058, Blast_Score=459, Evalue=1e-129,
Organism=Escherichia coli, GI1788013, Length=634, Percent_Identity=45.4258675078864, Blast_Score=610, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI71984184, Length=637, Percent_Identity=41.1302982731554, Blast_Score=529, Evalue=1e-150,
Organism=Caenorhabditis elegans, GI71984192, Length=637, Percent_Identity=41.1302982731554, Blast_Score=528, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6322112, Length=652, Percent_Identity=41.4110429447853, Blast_Score=526, Evalue=1e-150,
Organism=Saccharomyces cerevisiae, GI6322654, Length=392, Percent_Identity=41.8367346938776, Blast_Score=328, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24583842, Length=631, Percent_Identity=42.7892234548336, Blast_Score=546, Evalue=1e-155,
Organism=Drosophila melanogaster, GI28574141, Length=631, Percent_Identity=42.7892234548336, Blast_Score=546, Evalue=1e-155,
Organism=Drosophila melanogaster, GI24583839, Length=631, Percent_Identity=42.7892234548336, Blast_Score=545, Evalue=1e-155,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_NATTJ (B2A5P2)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917984.1
- ProteinModelPortal:   B2A5P2
- SMR:   B2A5P2
- GeneID:   6317006
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1824
- HOGENOM:   HBG352811
- OMA:   GFEYEVE
- ProtClustDB:   PRK00413
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 73550; Mature: 73550

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKITLKDGSVKEIEKGSNVLDLAKSISNRLAKEAVGAKVDGKTVGLNKELNENCEVEIL
CEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCEEEE
SFEDDEGCDIYRHTSSHILAHAVKRLYDNVKLGIGPPIKDGFYYDFDLEHSLSDKDLEDI
EECCCCCCCEEECCHHHHHHHHHHHHHCCCEECCCCCCCCCEEEEECCCCCCCCCCHHHH
KKEMKKIIKEDLPIERKVYKRDEAIEMFKEMGEDYKIELIQDLGEDEEICCYQQGDFIDL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCEEEECCCCEEEE
CQGPHLPSTGKIKDTALDLLKVAGAYWRGDENRAMLQRIYGTSFIKKSQLEEYIQRLEEA
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKREHRKLGKELDLFSFHEEAPGSPFFHNNGMIVINELIDFWRREHRKAGYQEISTPIIL
HHHHHHHHCCCCCHHCCCCCCCCCCEEECCCEEEHHHHHHHHHHHHHHCCHHHHCCCEEE
NRGLWERSGHWDHFQENMYFTEIDDEDFAVKPMNCPGAMLVYNNNMYSYRDFPLRVAELG
ECCCCCCCCCCCHHHCCEEEEEECCCCCEEECCCCCCEEEEEECCEEEECCCCCHHHHHH
KVHRHELSGALHGLLRARSFTQDDAHIFMLPSQVEEELENVINLVDRFYSVFGFNYYVEL
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
STKPEKAMGSDEIWDRAINALKSVLEKRGIKYIVNEGDGAFYGPKIDFQLEDAIGRSWQC
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCEECEEEHHHCCCCCCC
STIQLDFLLPERFDLTYIGEDGQKHRPVMIHRVIYGAIERFFALLIEHYEGKFPLWLAPR
EEEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC
QAVIIPIADRHMEYGKDITKQLESEGIRADLDQRNEKIGYKIRDAQLKKVPYMLIVGDDE
CEEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEECCHHCCCCEEEEECCCC
VENKTVSVRTREDGDIGSKSVAELVDEIKVEIDDMD
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHEECCCC
>Mature Secondary Structure
MLKITLKDGSVKEIEKGSNVLDLAKSISNRLAKEAVGAKVDGKTVGLNKELNENCEVEIL
CEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCEEEE
SFEDDEGCDIYRHTSSHILAHAVKRLYDNVKLGIGPPIKDGFYYDFDLEHSLSDKDLEDI
EECCCCCCCEEECCHHHHHHHHHHHHHCCCEECCCCCCCCCEEEEECCCCCCCCCCHHHH
KKEMKKIIKEDLPIERKVYKRDEAIEMFKEMGEDYKIELIQDLGEDEEICCYQQGDFIDL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCCEEEECCCCEEEE
CQGPHLPSTGKIKDTALDLLKVAGAYWRGDENRAMLQRIYGTSFIKKSQLEEYIQRLEEA
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKREHRKLGKELDLFSFHEEAPGSPFFHNNGMIVINELIDFWRREHRKAGYQEISTPIIL
HHHHHHHHCCCCCHHCCCCCCCCCCEEECCCEEEHHHHHHHHHHHHHHCCHHHHCCCEEE
NRGLWERSGHWDHFQENMYFTEIDDEDFAVKPMNCPGAMLVYNNNMYSYRDFPLRVAELG
ECCCCCCCCCCCHHHCCEEEEEECCCCCEEECCCCCCEEEEEECCEEEECCCCCHHHHHH
KVHRHELSGALHGLLRARSFTQDDAHIFMLPSQVEEELENVINLVDRFYSVFGFNYYVEL
HHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHCCEEEEEE
STKPEKAMGSDEIWDRAINALKSVLEKRGIKYIVNEGDGAFYGPKIDFQLEDAIGRSWQC
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEECCEECEEEHHHCCCCCCC
STIQLDFLLPERFDLTYIGEDGQKHRPVMIHRVIYGAIERFFALLIEHYEGKFPLWLAPR
EEEEEEEECCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC
QAVIIPIADRHMEYGKDITKQLESEGIRADLDQRNEKIGYKIRDAQLKKVPYMLIVGDDE
CEEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCCCEEEECCHHCCCCEEEEECCCC
VENKTVSVRTREDGDIGSKSVAELVDEIKVEIDDMD
CCCCEEEEEECCCCCCCHHHHHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA