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The map label for this gene is tapB [H]

Identifier: 188586348

GI number: 188586348

Start: 1796968

End: 1798653

Strand: Reverse

Name: tapB [H]

Synonym: Nther_1731

Alternate gene names: 188586348

Gene position: 1798653-1796968 (Counterclockwise)

Preceding gene: 188586349

Following gene: 188586347

Centisome position: 56.82

GC content: 32.56

Gene sequence:

>1686_bases
TTGATTGATTACAACATACTTTCAAAAGCTCAACTCAATGAAGCACTAATTGTTCAAAAGCGTACAGGTAACAGATTGAG
TTCGATAGTAATAGATTTACAATTTATAAGTGAGAATGCGTGGGTTCAATTGTTAGAAAAAAAATTAGGTTTACCGCGAA
TAGAATTGTCAGATTATTGTGTCGAAAATGATACTGCCTATATACTTCCCTTCCATATCGTTGAACAAAATCGAATATTT
CCATTTAAACTAAATCAGAATAATGTTGCTATAGCTACATCTGAACCATTGAATATTTTAATTATAGATGAAATTAAGTT
AATAACAGGTAAAGAAGTTGAAATATGGTTGGCTACTCCCGGAGAAATTGACAGAGAGATTAAAAGATTTTTTGATATCA
AAGAAGTGGTAGAAAAAGAAGTGCAGTTAATTTCCAAAGAATCTGTTACCAATATTATAGATAATAAAAACAGTGATCAA
AATTTAAATTTTGAAAATGAAGCTCCTGCTATAAAAATTATAGATACAGTAATAAAATTAGCCATTGATCAAGAAGCTAG
TGATATACATTTTGAACCAACTAGTAGTGACATGTTAGTTAGATTTAGAGTTGATGGGATGATGCGTCAAATAACCTGTT
TTCCCAAGCACACTCAAGAACTGTTGATATCACGGATAAAAATAATGACCAATATGGATATAACTGTGAAAAGATTACCG
CAAGATGGACGATGTGAATTTACAAGTCAAGAACAGAACATAGATTTAAGAGCATCAGCACTACCCACTATATTTGGTGA
AAAAATAGTGATCAGATTACTTTACAAAGAAAAAATTATTTTTCCTTTGGACAAACTTGGGTTTAAAGATAAAGAATTAA
GTCAATACAAGGCTATGTTGGCTGATAGTTCTGGCATGATATTAGTGACTGGACCTACGGGCTGTGGAAAGACAACGACT
TTATACTCATCACTTAGTTGGATAAATTCACCAGAAAAAAATATAATTACTGTGGAAGACCCTGTAGAATACCAACTACC
AGGTGTTAATCAAGTGGAAATGAATGAGAAAGCTGGATTAACCTTCGCAATTGGATTAAGGTCTATTCTACGGCAAGATC
CCGATATAATTATGATAGGTGAAATCAGAGATTTGGAAACGGCACGGATAGCAGTCAGATCCTCATTGACTGGTCATTTA
GTTTTTTCCACACTACACACTTCAAATTCAATAGGAGCTTTATCAAGATTAATTGATATGGGGATTCCCATGTATTTATT
GAACTCTTCTATTAAAGGTATTTTGTCTCAGAGATTGGTTAGGAAAATTTGTAATCATTGTAAAGAACTATATCAGCCTA
GTTTTCAAGAACTTAACTTTTATGAAGCTCATGCCAATCATATATCTTATTTACCTAATTTTTTTAGAGGCAGAGGTTGT
AGAATATGTAATGGAACAGGATATCAAGGGAGAAAGGCTGTATTTGAACTGCTAGTAGTAGACAATCAAATAAAAAAAGA
AATCGCGAAAGGGGTAACAAAACTAGATGACTTGAACTTCGCGTCAGTGTGGGATTGTTTAACACTAGTGAATAATGCTT
TACAGGATGTTAATAGTGGAATTACGACTTTAGAAGAAGTTATTAGAATAACAAGTTTCCAAGAATCCCTAAAAGTGAGG
AAATAA

Upstream 100 bases:

>100_bases
AGTAAAGAGGGTTTTGGAAAATTATGTAGAATAAAGTATTACTTTCACTTGTCGGAGGTGCATAACAATTTCAAACAACC
AGATCAGTATAGAAAAACTA

Downstream 100 bases:

>100_bases
GATGATTAAATATTATTATAGAGCAAAAAGTTGGACAGGAGAAGAAATTAAGGGATTTATCACTGCTGACAGTCGGGAAA
CAGCCATATTAAATTTAACA

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 561; Mature: 561

Protein sequence:

>561_residues
MIDYNILSKAQLNEALIVQKRTGNRLSSIVIDLQFISENAWVQLLEKKLGLPRIELSDYCVENDTAYILPFHIVEQNRIF
PFKLNQNNVAIATSEPLNILIIDEIKLITGKEVEIWLATPGEIDREIKRFFDIKEVVEKEVQLISKESVTNIIDNKNSDQ
NLNFENEAPAIKIIDTVIKLAIDQEASDIHFEPTSSDMLVRFRVDGMMRQITCFPKHTQELLISRIKIMTNMDITVKRLP
QDGRCEFTSQEQNIDLRASALPTIFGEKIVIRLLYKEKIIFPLDKLGFKDKELSQYKAMLADSSGMILVTGPTGCGKTTT
LYSSLSWINSPEKNIITVEDPVEYQLPGVNQVEMNEKAGLTFAIGLRSILRQDPDIIMIGEIRDLETARIAVRSSLTGHL
VFSTLHTSNSIGALSRLIDMGIPMYLLNSSIKGILSQRLVRKICNHCKELYQPSFQELNFYEAHANHISYLPNFFRGRGC
RICNGTGYQGRKAVFELLVVDNQIKKEIAKGVTKLDDLNFASVWDCLTLVNNALQDVNSGITTLEEVIRITSFQESLKVR
K

Sequences:

>Translated_561_residues
MIDYNILSKAQLNEALIVQKRTGNRLSSIVIDLQFISENAWVQLLEKKLGLPRIELSDYCVENDTAYILPFHIVEQNRIF
PFKLNQNNVAIATSEPLNILIIDEIKLITGKEVEIWLATPGEIDREIKRFFDIKEVVEKEVQLISKESVTNIIDNKNSDQ
NLNFENEAPAIKIIDTVIKLAIDQEASDIHFEPTSSDMLVRFRVDGMMRQITCFPKHTQELLISRIKIMTNMDITVKRLP
QDGRCEFTSQEQNIDLRASALPTIFGEKIVIRLLYKEKIIFPLDKLGFKDKELSQYKAMLADSSGMILVTGPTGCGKTTT
LYSSLSWINSPEKNIITVEDPVEYQLPGVNQVEMNEKAGLTFAIGLRSILRQDPDIIMIGEIRDLETARIAVRSSLTGHL
VFSTLHTSNSIGALSRLIDMGIPMYLLNSSIKGILSQRLVRKICNHCKELYQPSFQELNFYEAHANHISYLPNFFRGRGC
RICNGTGYQGRKAVFELLVVDNQIKKEIAKGVTKLDDLNFASVWDCLTLVNNALQDVNSGITTLEEVIRITSFQESLKVR
K
>Mature_561_residues
MIDYNILSKAQLNEALIVQKRTGNRLSSIVIDLQFISENAWVQLLEKKLGLPRIELSDYCVENDTAYILPFHIVEQNRIF
PFKLNQNNVAIATSEPLNILIIDEIKLITGKEVEIWLATPGEIDREIKRFFDIKEVVEKEVQLISKESVTNIIDNKNSDQ
NLNFENEAPAIKIIDTVIKLAIDQEASDIHFEPTSSDMLVRFRVDGMMRQITCFPKHTQELLISRIKIMTNMDITVKRLP
QDGRCEFTSQEQNIDLRASALPTIFGEKIVIRLLYKEKIIFPLDKLGFKDKELSQYKAMLADSSGMILVTGPTGCGKTTT
LYSSLSWINSPEKNIITVEDPVEYQLPGVNQVEMNEKAGLTFAIGLRSILRQDPDIIMIGEIRDLETARIAVRSSLTGHL
VFSTLHTSNSIGALSRLIDMGIPMYLLNSSIKGILSQRLVRKICNHCKELYQPSFQELNFYEAHANHISYLPNFFRGRGC
RICNGTGYQGRKAVFELLVVDNQIKKEIAKGVTKLDDLNFASVWDCLTLVNNALQDVNSGITTLEEVIRITSFQESLKVR
K

Specific function: Involved in the translocation of the type IV pilin [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=391, Percent_Identity=45.2685421994885, Blast_Score=355, Evalue=5e-99,
Organism=Escherichia coli, GI1786296, Length=388, Percent_Identity=39.1752577319588, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI87082188, Length=278, Percent_Identity=33.8129496402878, Blast_Score=131, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 63632; Mature: 63632

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDYNILSKAQLNEALIVQKRTGNRLSSIVIDLQFISENAWVQLLEKKLGLPRIELSDYC
CCCCCCCCHHHCCCEEEEEECCCCCEEHHEEEEEECCCHHHHHHHHHHHCCCCEECCCEE
VENDTAYILPFHIVEQNRIFPFKLNQNNVAIATSEPLNILIIDEIKLITGKEVEIWLATP
ECCCCEEEEEEEEECCCCEEEEEECCCCEEEEECCCEEEEEEEEEEEECCCEEEEEEECC
GEIDREIKRFFDIKEVVEKEVQLISKESVTNIIDNKNSDQNLNFENEAPAIKIIDTVIKL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
AIDQEASDIHFEPTSSDMLVRFRVDGMMRQITCFPKHTQELLISRIKIMTNMDITVKRLP
HHCCCCCCEEECCCCCCEEEEEECCCHHHHHEECCCHHHHHHHHHHHHHCCCCEEEEECC
QDGRCEFTSQEQNIDLRASALPTIFGEKIVIRLLYKEKIIFPLDKLGFKDKELSQYKAML
CCCCEEECCCCCCCCEEHHHCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHH
ADSSGMILVTGPTGCGKTTTLYSSLSWINSPEKNIITVEDPVEYQLPGVNQVEMNEKAGL
CCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCEEECCCCCCC
TFAIGLRSILRQDPDIIMIGEIRDLETARIAVRSSLTGHLVFSTLHTSNSIGALSRLIDM
EEEHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHC
GIPMYLLNSSIKGILSQRLVRKICNHCKELYQPSFQELNFYEAHANHISYLPNFFRGRGC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHCCHHHCCCCC
RICNGTGYQGRKAVFELLVVDNQIKKEIAKGVTKLDDLNFASVWDCLTLVNNALQDVNSG
EEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
ITTLEEVIRITSFQESLKVRK
HHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIDYNILSKAQLNEALIVQKRTGNRLSSIVIDLQFISENAWVQLLEKKLGLPRIELSDYC
CCCCCCCCHHHCCCEEEEEECCCCCEEHHEEEEEECCCHHHHHHHHHHHCCCCEECCCEE
VENDTAYILPFHIVEQNRIFPFKLNQNNVAIATSEPLNILIIDEIKLITGKEVEIWLATP
ECCCCEEEEEEEEECCCCEEEEEECCCCEEEEECCCEEEEEEEEEEEECCCEEEEEEECC
GEIDREIKRFFDIKEVVEKEVQLISKESVTNIIDNKNSDQNLNFENEAPAIKIIDTVIKL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
AIDQEASDIHFEPTSSDMLVRFRVDGMMRQITCFPKHTQELLISRIKIMTNMDITVKRLP
HHCCCCCCEEECCCCCCEEEEEECCCHHHHHEECCCHHHHHHHHHHHHHCCCCEEEEECC
QDGRCEFTSQEQNIDLRASALPTIFGEKIVIRLLYKEKIIFPLDKLGFKDKELSQYKAML
CCCCEEECCCCCCCCEEHHHCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCHHHHHHHHHH
ADSSGMILVTGPTGCGKTTTLYSSLSWINSPEKNIITVEDPVEYQLPGVNQVEMNEKAGL
CCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCEEECCCCCEEECCCCCCC
TFAIGLRSILRQDPDIIMIGEIRDLETARIAVRSSLTGHLVFSTLHTSNSIGALSRLIDM
EEEHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHC
GIPMYLLNSSIKGILSQRLVRKICNHCKELYQPSFQELNFYEAHANHISYLPNFFRGRGC
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHCCHHHCCCCC
RICNGTGYQGRKAVFELLVVDNQIKKEIAKGVTKLDDLNFASVWDCLTLVNNALQDVNSG
EEECCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
ITTLEEVIRITSFQESLKVRK
HHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8820654 [H]