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The map label for this gene is folD

Identifier: 188586317

GI number: 188586317

Start: 1775183

End: 1776037

Strand: Reverse

Name: folD

Synonym: Nther_1700

Alternate gene names: 188586317

Gene position: 1776037-1775183 (Counterclockwise)

Preceding gene: 188586318

Following gene: 188586316

Centisome position: 56.11

GC content: 39.3

Gene sequence:

>855_bases
GTGAGTGCGAGTTTAATAAAAGGTTCAGATGTGGCTAAGGAAATTCGCTCCGATTTACAAAAGGATGTTGAGGATTTAAA
ATCTCAAGGCGTCACACCACATTTGGCTGTTGTCTTGGTGGGAGAAGATTCGGCTTCACAGACTTATGTGAATATGAAAG
AAAAAACTGCAGGTAAAGTGGGAATTGAATCAACGGTTAAAAGATTACCAACTAACGTTGAAGAAGATGAACTATTGCGA
TTGATAGATGAGCTTAATAAAGATGACAGTGTACACGGTATTTTAGTTCAACTTCCGCTTCCTTATCATATTGACGAATA
TAAAATCCTTAATGCAATAGATCAATATAAAGATGTGGATAGTTTTCATCCAGTAAATGTAGGAAACCTTACCATTGGAC
AAAAGAGGTTTACTCCATGTACCCCAGCCGGTATCATGAAGTTATTGGATTCCATCGATTATGATTTGAACGGTAAACAT
GCAGTGGTATTGGGTAGAAGTAATATAGTAGGCAAACCAATATCTCTGCTCTTGTTAGAACGAAATGCGACTGTATCTAT
CTGTCATTCCAGGACAAAGGACCTGACAGAAATGACAAAATCCGCCGATCTTTTGATAGTTGCTGTAGGGCGGCCTGAGA
TAGTAACTGGAAAAATGATTAAACCAGGAGCTGTAGTGATTGATGTGGGAGTTAATAAAGTTGATGATAAGCTAATCGGG
GATGTTGAATTTGAATCTGCCAAAGAAGTTGCTGGATGGATTACACCTGTACCAGGCGGTGTTGGCCCTATGACCATCAC
AATGCTTTTAAATAATACCATCGAGGCTGCCAGGAGATGTCTGAACGAACAGTAA

Upstream 100 bases:

>100_bases
ATTGATTCATACAGCTTACGCACAAATTAACAGAGTTTTTAATTAAATAAGGTTAAATGAAGGGGAAGATATTAAGAGTT
ACTTAAAAGGAGGTATTTTT

Downstream 100 bases:

>100_bases
CGGTTTCGCAACTAACGAATTATCTTAAAAACCTGTTACTACAAGATCATAATCTTAAAAATGTCCTGGTGAGAGGTGAG
ATTTCTAATTTTAAACACCA

Product: 5,10-methylenetetrahydrofolate dehydrogenase (NADP+), Methenyltetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSASLIKGSDVAKEIRSDLQKDVEDLKSQGVTPHLAVVLVGEDSASQTYVNMKEKTAGKVGIESTVKRLPTNVEEDELLR
LIDELNKDDSVHGILVQLPLPYHIDEYKILNAIDQYKDVDSFHPVNVGNLTIGQKRFTPCTPAGIMKLLDSIDYDLNGKH
AVVLGRSNIVGKPISLLLLERNATVSICHSRTKDLTEMTKSADLLIVAVGRPEIVTGKMIKPGAVVIDVGVNKVDDKLIG
DVEFESAKEVAGWITPVPGGVGPMTITMLLNNTIEAARRCLNEQ

Sequences:

>Translated_284_residues
MSASLIKGSDVAKEIRSDLQKDVEDLKSQGVTPHLAVVLVGEDSASQTYVNMKEKTAGKVGIESTVKRLPTNVEEDELLR
LIDELNKDDSVHGILVQLPLPYHIDEYKILNAIDQYKDVDSFHPVNVGNLTIGQKRFTPCTPAGIMKLLDSIDYDLNGKH
AVVLGRSNIVGKPISLLLLERNATVSICHSRTKDLTEMTKSADLLIVAVGRPEIVTGKMIKPGAVVIDVGVNKVDDKLIG
DVEFESAKEVAGWITPVPGGVGPMTITMLLNNTIEAARRCLNEQ
>Mature_283_residues
SASLIKGSDVAKEIRSDLQKDVEDLKSQGVTPHLAVVLVGEDSASQTYVNMKEKTAGKVGIESTVKRLPTNVEEDELLRL
IDELNKDDSVHGILVQLPLPYHIDEYKILNAIDQYKDVDSFHPVNVGNLTIGQKRFTPCTPAGIMKLLDSIDYDLNGKHA
VVLGRSNIVGKPISLLLLERNATVSICHSRTKDLTEMTKSADLLIVAVGRPEIVTGKMIKPGAVVIDVGVNKVDDKLIGD
VEFESAKEVAGWITPVPGGVGPMTITMLLNNTIEAARRCLNEQ

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI222136639, Length=294, Percent_Identity=45.578231292517, Blast_Score=255, Evalue=4e-68,
Organism=Homo sapiens, GI222418558, Length=296, Percent_Identity=46.2837837837838, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=46.9594594594595, Blast_Score=235, Evalue=3e-62,
Organism=Homo sapiens, GI36796743, Length=276, Percent_Identity=26.0869565217391, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1786741, Length=279, Percent_Identity=49.4623655913978, Blast_Score=260, Evalue=7e-71,
Organism=Caenorhabditis elegans, GI17568735, Length=292, Percent_Identity=43.4931506849315, Blast_Score=225, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6319558, Length=290, Percent_Identity=47.2413793103448, Blast_Score=264, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6321643, Length=302, Percent_Identity=42.7152317880795, Blast_Score=244, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6322933, Length=319, Percent_Identity=27.8996865203762, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17136816, Length=299, Percent_Identity=45.819397993311, Blast_Score=263, Evalue=8e-71,
Organism=Drosophila melanogaster, GI17136818, Length=299, Percent_Identity=45.819397993311, Blast_Score=263, Evalue=1e-70,
Organism=Drosophila melanogaster, GI62472483, Length=291, Percent_Identity=47.0790378006873, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI45551871, Length=291, Percent_Identity=47.0790378006873, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24645718, Length=291, Percent_Identity=47.0790378006873, Blast_Score=249, Evalue=1e-66,
Organism=Drosophila melanogaster, GI17137370, Length=291, Percent_Identity=47.0790378006873, Blast_Score=249, Evalue=1e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_NATTJ (B2A532)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001917862.1
- ProteinModelPortal:   B2A532
- SMR:   B2A532
- GeneID:   6317044
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_1700
- HOGENOM:   HBG328751
- OMA:   DVVYETA
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30885; Mature: 30754

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASLIKGSDVAKEIRSDLQKDVEDLKSQGVTPHLAVVLVGEDSASQTYVNMKEKTAGKV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEECHHHCCCCC
GIESTVKRLPTNVEEDELLRLIDELNKDDSVHGILVQLPLPYHIDEYKILNAIDQYKDVD
CHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCC
SFHPVNVGNLTIGQKRFTPCTPAGIMKLLDSIDYDLNGKHAVVLGRSNIVGKPISLLLLE
CCCCCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEEEEE
RNATVSICHSRTKDLTEMTKSADLLIVAVGRPEIVTGKMIKPGAVVIDVGVNKVDDKLIG
CCCEEEEHHHHHHHHHHHHCCCCEEEEEECCCCEEECCEECCCEEEEECCCCHHHHHHCC
DVEFESAKEVAGWITPVPGGVGPMTITMLLNNTIEAARRCLNEQ
CCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SASLIKGSDVAKEIRSDLQKDVEDLKSQGVTPHLAVVLVGEDSASQTYVNMKEKTAGKV
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEEECHHHCCCCC
GIESTVKRLPTNVEEDELLRLIDELNKDDSVHGILVQLPLPYHIDEYKILNAIDQYKDVD
CHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCC
SFHPVNVGNLTIGQKRFTPCTPAGIMKLLDSIDYDLNGKHAVVLGRSNIVGKPISLLLLE
CCCCCCCCCEEECCCCCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCEEEEEEE
RNATVSICHSRTKDLTEMTKSADLLIVAVGRPEIVTGKMIKPGAVVIDVGVNKVDDKLIG
CCCEEEEHHHHHHHHHHHHCCCCEEEEEECCCCEEECCEECCCEEEEECCCCHHHHHHCC
DVEFESAKEVAGWITPVPGGVGPMTITMLLNNTIEAARRCLNEQ
CCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA