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The map label for this gene is ldh [H]

Identifier: 188586298

GI number: 188586298

Start: 1755752

End: 1756816

Strand: Reverse

Name: ldh [H]

Synonym: Nther_1681

Alternate gene names: 188586298

Gene position: 1756816-1755752 (Counterclockwise)

Preceding gene: 188586299

Following gene: 188586297

Centisome position: 55.5

GC content: 37.0

Gene sequence:

>1065_bases
ATGAAGATTTTAGAGACAATGAAAGCTTCAGATTATGAACAACTGGTATTTTGTCAGGACGAAAAGACTGGTCTTAAAGG
AATTATTGCTATCCATGATACCACACTTGGACCAGCCCTTGGCGGCACTAGAATGTGGACCTATGACAATGAAGAAGAAG
CTATTGAAGACGTGCTAAGACTAGCAAGAGGTATGACTTACAAATCAGCTGCAGCTGGATTGAACCTTGGTGGAGGAAAA
GGTGTTATTATTGGTGATCCTAAAAAAGACAAATCGGAAGAAATGTGGAGAGCTTTTGGTAGATTTGTTCAATCATTAAA
CGGACGATATATAACTGCTGAAGATGTGGGTGTTAGAGAAACAGACTTGGAAATTGTTAATACTGAAACTGATTTTGCTG
TTGGACTACCAGGTAAGAGTGGCAACCCTTCACCTGCTACTGCTTATGGAGTTTATTCAGGTATAAAAGCTGTTGCTGAT
GAAATTTGGGGAAGTGCAGATCTAAATGGAAAAACTATTGCTATACAGGGTGCAGGTAGTGTAGGATATTACTTAAGTGA
GCTACTTCATAAAGATGGAGCAAAATTAATTGTCACTGATATAGATAAAGAGGCTGTAGACAAGTTAGTTTCAGACTTTG
GTGCCACTGCTGTGGAAACTGATGAAATCTATGAACAAGAAGCTGATATTTTTGCCCCTTGTGCATTAGGTGCTATCTTA
AACGATGAAACTATTCCAAAATTAAAAGTAAAAGCGGTGGCTGGAGCTGCTAATAATCAGCTAGAAGATGAGAAACGCCA
TGCTGAAGAATTGAAAAAACGAGGGATAGTTTATGCTCCTGATTATGTAATTAATGCCGGTGGAGTTATTAATGTCTCCT
TCGAATTAACTGGATATGATGAGGAAAGAGCATATAGAAAAATCTCTACTATATATGACAATATTAAGAAAATCTTTAAT
ATAGCAAATAGAGATGATATCACATCTCATGAAGCTGCCAACAGAATGGCAGAAGAACGAATCGAAGCGATTAAGCATGT
AAAAACTAGTTATATTAATAAATAA

Upstream 100 bases:

>100_bases
CCAAAGTTTATTCTATTGCTGCCGCGTGTATGATGATGGCTAATAGAAAGTAATAGGAAGTAAATTTTTACTAAAATTAT
TAATCAAGGAGGTAGTTTTT

Downstream 100 bases:

>100_bases
ATTACTAATCATTTGGAGGGTTGCAATATGAAACAAGAACATCGCTTGTTAGTAATAAATCCTGGATCCACCTCCACAAA
AATAGCTGTCTATGAAGGTG

Product: leucine dehydrogenase

Products: NA

Alternate protein names: LeuDH [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MKILETMKASDYEQLVFCQDEKTGLKGIIAIHDTTLGPALGGTRMWTYDNEEEAIEDVLRLARGMTYKSAAAGLNLGGGK
GVIIGDPKKDKSEEMWRAFGRFVQSLNGRYITAEDVGVRETDLEIVNTETDFAVGLPGKSGNPSPATAYGVYSGIKAVAD
EIWGSADLNGKTIAIQGAGSVGYYLSELLHKDGAKLIVTDIDKEAVDKLVSDFGATAVETDEIYEQEADIFAPCALGAIL
NDETIPKLKVKAVAGAANNQLEDEKRHAEELKKRGIVYAPDYVINAGGVINVSFELTGYDEERAYRKISTIYDNIKKIFN
IANRDDITSHEAANRMAEERIEAIKHVKTSYINK

Sequences:

>Translated_354_residues
MKILETMKASDYEQLVFCQDEKTGLKGIIAIHDTTLGPALGGTRMWTYDNEEEAIEDVLRLARGMTYKSAAAGLNLGGGK
GVIIGDPKKDKSEEMWRAFGRFVQSLNGRYITAEDVGVRETDLEIVNTETDFAVGLPGKSGNPSPATAYGVYSGIKAVAD
EIWGSADLNGKTIAIQGAGSVGYYLSELLHKDGAKLIVTDIDKEAVDKLVSDFGATAVETDEIYEQEADIFAPCALGAIL
NDETIPKLKVKAVAGAANNQLEDEKRHAEELKKRGIVYAPDYVINAGGVINVSFELTGYDEERAYRKISTIYDNIKKIFN
IANRDDITSHEAANRMAEERIEAIKHVKTSYINK
>Mature_354_residues
MKILETMKASDYEQLVFCQDEKTGLKGIIAIHDTTLGPALGGTRMWTYDNEEEAIEDVLRLARGMTYKSAAAGLNLGGGK
GVIIGDPKKDKSEEMWRAFGRFVQSLNGRYITAEDVGVRETDLEIVNTETDFAVGLPGKSGNPSPATAYGVYSGIKAVAD
EIWGSADLNGKTIAIQGAGSVGYYLSELLHKDGAKLIVTDIDKEAVDKLVSDFGATAVETDEIYEQEADIFAPCALGAIL
NDETIPKLKVKAVAGAANNQLEDEKRHAEELKKRGIVYAPDYVINAGGVINVSFELTGYDEERAYRKISTIYDNIKKIFN
IANRDDITSHEAANRMAEERIEAIKHVKTSYINK

Specific function: Functions catabolically in the bacterial metabolism of branched-chain L-amino acids, and plays an important role in spore germination in cooperation with alanine dehydrogenase [H]

COG id: COG0334

COG function: function code E; Glutamate dehydrogenase/leucine dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Glu/Leu/Phe/Val dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI31377775, Length=335, Percent_Identity=25.6716417910448, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI4885281, Length=335, Percent_Identity=25.3731343283582, Blast_Score=72, Evalue=9e-13,
Organism=Drosophila melanogaster, GI45549226, Length=355, Percent_Identity=25.6338028169014, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45553475, Length=371, Percent_Identity=24.7978436657682, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24649283, Length=318, Percent_Identity=24.8427672955975, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24649460, Length=315, Percent_Identity=25.7142857142857, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 9362 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006095
- InterPro:   IPR006096
- InterPro:   IPR006097
- InterPro:   IPR016211
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00208 ELFV_dehydrog; PF02812 ELFV_dehydrog_N [H]

EC number: =1.4.1.9 [H]

Molecular weight: Translated: 38641; Mature: 38641

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00074 GLFV_DEHYDROGENASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILETMKASDYEQLVFCQDEKTGLKGIIAIHDTTLGPALGGTRMWTYDNEEEAIEDVLR
CCHHHHHCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCEEEECCCHHHHHHHHHH
LARGMTYKSAAAGLNLGGGKGVIIGDPKKDKSEEMWRAFGRFVQSLNGRYITAEDVGVRE
HHHCCCHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEE
TDLEIVNTETDFAVGLPGKSGNPSPATAYGVYSGIKAVADEIWGSADLNGKTIAIQGAGS
CCCEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCC
VGYYLSELLHKDGAKLIVTDIDKEAVDKLVSDFGATAVETDEIYEQEADIFAPCALGAIL
HHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCEEHHHHHHHCCCHHHHHHHHHHH
NDETIPKLKVKAVAGAANNQLEDEKRHAEELKKRGIVYAPDYVINAGGVINVSFELTGYD
CCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEECCCEEEECCCEEEEEEEEECCC
EERAYRKISTIYDNIKKIFNIANRDDITSHEAANRMAEERIEAIKHVKTSYINK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKILETMKASDYEQLVFCQDEKTGLKGIIAIHDTTLGPALGGTRMWTYDNEEEAIEDVLR
CCHHHHHCCCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCEEEECCCHHHHHHHHHH
LARGMTYKSAAAGLNLGGGKGVIIGDPKKDKSEEMWRAFGRFVQSLNGRYITAEDVGVRE
HHHCCCHHHHHCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEE
TDLEIVNTETDFAVGLPGKSGNPSPATAYGVYSGIKAVADEIWGSADLNGKTIAIQGAGS
CCCEEEECCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCC
VGYYLSELLHKDGAKLIVTDIDKEAVDKLVSDFGATAVETDEIYEQEADIFAPCALGAIL
HHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHCCCCEEHHHHHHHCCCHHHHHHHHHHH
NDETIPKLKVKAVAGAANNQLEDEKRHAEELKKRGIVYAPDYVINAGGVINVSFELTGYD
CCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEECCCEEEECCCEEEEEEEEECCC
EERAYRKISTIYDNIKKIFNIANRDDITSHEAANRMAEERIEAIKHVKTSYINK
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3069133; 1400267 [H]