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The map label for this gene is buk1 [H]

Identifier: 188586297

GI number: 188586297

Start: 1754648

End: 1755724

Strand: Reverse

Name: buk1 [H]

Synonym: Nther_1680

Alternate gene names: 188586297

Gene position: 1755724-1754648 (Counterclockwise)

Preceding gene: 188586298

Following gene: 188586296

Centisome position: 55.46

GC content: 37.14

Gene sequence:

>1077_bases
ATGAAACAAGAACATCGCTTGTTAGTAATAAATCCTGGATCCACCTCCACAAAAATAGCTGTCTATGAAGGTGAAAGCCC
ATTAATTGAAAAGAAACTCGAACATTCACCAGAGGAATTAAATAAGTTTCAAGACATTATAGACCAATATGACTTTAGAA
AAGATAGTATTCTGAACTTTCTAGATGAACAGGGCATGAACTTTAATAAACTAGATGCAGTTGTCGCCAGAGGTGGGCTG
TTAAAGCCTATCTCTGGTGGTACATTCAAAGTGAATGATTTGATGGTCGAACATCTAAGACAGGGTTATCAAGGAGAGCA
CGCTTCCAATTTGGGAGGTATTATAGCTAAAGAAATATCTAATCAACTTGATATTCCAGCCTATATTGTTGATCCAGTGG
TAGTTGATGAATTACAGGATATTGCACGTATATCAGGGTTTGAGCCAATTGAAAGAAAAAGCATTTTCCATGCATTAAAT
CACAAAGCAGTTGCCAGAAAAGCAGCAGCTCAACTAGGTAAAAAATATGAAGAAGCTAATTTAGTAGTAGTTCATTTAGG
TGGAGGTATCTCCGTGGGGGCGCATAATTGCGGTGATGTCATCGATGTTAATAATGCCCTCGATGGAGAAGGACCCTTTT
CACCAGAACGAAGTGGTGGACTCCCTAATGGTGATTTAGTACGATATATAGATGAGCACAATTTAACCTGGAAAGAATTA
AAACGACAGTTGGTTGGTAACGGTGGACTAGTATCTTATTTAGATACCAATGATGGTAAAAAAGTTCAAGAGATGATACA
ATCAGGCGATGAGAAAGCTGAAAAAGTATATGAAGCCATGATCTATCAAATAGCTAAAGAGATAGGTTCCTGTGCTGTTG
TATTAAAAGGTGAATTAGATGCTATTGTATTAACTGGTGGATTAGCTCATGATGAATATCTAGTTTCTAGAATGAAGGAA
TATATTAAATTCTTGGGTGAAATTCTAGTCTTTCCTGGAGAAGATGAGATGCAATCCTTGGCCGAAGGTGGCTTAAGAGT
CTTAAGAAATGAAGAAACTCCGAAAGATTACTCTTAA

Upstream 100 bases:

>100_bases
AGCTGCCAACAGAATGGCAGAAGAACGAATCGAAGCGATTAAGCATGTAAAAACTAGTTATATTAATAAATAAATTACTA
ATCATTTGGAGGGTTGCAAT

Downstream 100 bases:

>100_bases
TTATGAAAGTATTGGTGATCTTATGACAAAATATCCCAAAATTCCTGAAACAGACATTATTGTATTTGCCGGACATTTCG
GAAGCGGTAAAACAGAAAAT

Product: butyrate kinase

Products: NA

Alternate protein names: BK 1; Branched-chain carboxylic acid kinase 1 [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MKQEHRLLVINPGSTSTKIAVYEGESPLIEKKLEHSPEELNKFQDIIDQYDFRKDSILNFLDEQGMNFNKLDAVVARGGL
LKPISGGTFKVNDLMVEHLRQGYQGEHASNLGGIIAKEISNQLDIPAYIVDPVVVDELQDIARISGFEPIERKSIFHALN
HKAVARKAAAQLGKKYEEANLVVVHLGGGISVGAHNCGDVIDVNNALDGEGPFSPERSGGLPNGDLVRYIDEHNLTWKEL
KRQLVGNGGLVSYLDTNDGKKVQEMIQSGDEKAEKVYEAMIYQIAKEIGSCAVVLKGELDAIVLTGGLAHDEYLVSRMKE
YIKFLGEILVFPGEDEMQSLAEGGLRVLRNEETPKDYS

Sequences:

>Translated_358_residues
MKQEHRLLVINPGSTSTKIAVYEGESPLIEKKLEHSPEELNKFQDIIDQYDFRKDSILNFLDEQGMNFNKLDAVVARGGL
LKPISGGTFKVNDLMVEHLRQGYQGEHASNLGGIIAKEISNQLDIPAYIVDPVVVDELQDIARISGFEPIERKSIFHALN
HKAVARKAAAQLGKKYEEANLVVVHLGGGISVGAHNCGDVIDVNNALDGEGPFSPERSGGLPNGDLVRYIDEHNLTWKEL
KRQLVGNGGLVSYLDTNDGKKVQEMIQSGDEKAEKVYEAMIYQIAKEIGSCAVVLKGELDAIVLTGGLAHDEYLVSRMKE
YIKFLGEILVFPGEDEMQSLAEGGLRVLRNEETPKDYS
>Mature_358_residues
MKQEHRLLVINPGSTSTKIAVYEGESPLIEKKLEHSPEELNKFQDIIDQYDFRKDSILNFLDEQGMNFNKLDAVVARGGL
LKPISGGTFKVNDLMVEHLRQGYQGEHASNLGGIIAKEISNQLDIPAYIVDPVVVDELQDIARISGFEPIERKSIFHALN
HKAVARKAAAQLGKKYEEANLVVVHLGGGISVGAHNCGDVIDVNNALDGEGPFSPERSGGLPNGDLVRYIDEHNLTWKEL
KRQLVGNGGLVSYLDTNDGKKVQEMIQSGDEKAEKVYEAMIYQIAKEIGSCAVVLKGELDAIVLTGGLAHDEYLVSRMKE
YIKFLGEILVFPGEDEMQSLAEGGLRVLRNEETPKDYS

Specific function: Anaerobic degradation of L-threonine to propionate. [C]

COG id: COG3426

COG function: function code C; Butyrate kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acetokinase family [H]

Homologues:

Organism=Escherichia coli, GI145693184, Length=178, Percent_Identity=27.5280898876405, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788633, Length=169, Percent_Identity=28.4023668639053, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000890
- InterPro:   IPR011245 [H]

Pfam domain/function: PF00871 Acetate_kinase [H]

EC number: =2.7.2.7 [H]

Molecular weight: Translated: 39499; Mature: 39499

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS01075 ACETATE_KINASE_1 ; PS01076 ACETATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQEHRLLVINPGSTSTKIAVYEGESPLIEKKLEHSPEELNKFQDIIDQYDFRKDSILNF
CCCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHH
LDEQGMNFNKLDAVVARGGLLKPISGGTFKVNDLMVEHLRQGYQGEHASNLGGIIAKEIS
HHHCCCCHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
NQLDIPAYIVDPVVVDELQDIARISGFEPIERKSIFHALNHKAVARKAAAQLGKKYEEAN
CCCCCCHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LVVVHLGGGISVGAHNCGDVIDVNNALDGEGPFSPERSGGLPNGDLVRYIDEHNLTWKEL
EEEEEECCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHH
KRQLVGNGGLVSYLDTNDGKKVQEMIQSGDEKAEKVYEAMIYQIAKEIGSCAVVLKGELD
HHHHCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
AIVLTGGLAHDEYLVSRMKEYIKFLGEILVFPGEDEMQSLAEGGLRVLRNEETPKDYS
EEEEECCCCHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKQEHRLLVINPGSTSTKIAVYEGESPLIEKKLEHSPEELNKFQDIIDQYDFRKDSILNF
CCCCCEEEEECCCCCCEEEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHH
LDEQGMNFNKLDAVVARGGLLKPISGGTFKVNDLMVEHLRQGYQGEHASNLGGIIAKEIS
HHHCCCCHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
NQLDIPAYIVDPVVVDELQDIARISGFEPIERKSIFHALNHKAVARKAAAQLGKKYEEAN
CCCCCCHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
LVVVHLGGGISVGAHNCGDVIDVNNALDGEGPFSPERSGGLPNGDLVRYIDEHNLTWKEL
EEEEEECCCEEECCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHH
KRQLVGNGGLVSYLDTNDGKKVQEMIQSGDEKAEKVYEAMIYQIAKEIGSCAVVLKGELD
HHHHCCCCCEEEEEECCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCEEEEEECCCC
AIVLTGGLAHDEYLVSRMKEYIKFLGEILVFPGEDEMQSLAEGGLRVLRNEETPKDYS
EEEEECCCCHHHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]