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The map label for this gene is appA [H]

Identifier: 188586213

GI number: 188586213

Start: 1671840

End: 1673558

Strand: Reverse

Name: appA [H]

Synonym: Nther_1596

Alternate gene names: 188586213

Gene position: 1673558-1671840 (Counterclockwise)

Preceding gene: 188586214

Following gene: 188586212

Centisome position: 52.87

GC content: 37.52

Gene sequence:

>1719_bases
ATGTCAAGTCGAAAAATATTTGTTTTGTTTATTGCTTTTCTCCTTACAATGGGAGTGGCTTTGAGTGCTTGTGCACCTCC
AGAAGAGGCTAAAGATGAAGAAGTGGAAGAAAAAGAAGACGAAGAACCCGTAGTTGAAAACCCTGCAGTTGAACGTCCTA
ATGAATTAATTATCGGAGGAACTGATTTAGATGGAATTTTTAATCCAGTTCTTTACTCTACGGCATATGATGCTTGGGTT
ATAGGAATGATGTATGATACATTGCTTACAGTAGACGAAAATGGTGAATTAACGACGGATCAGAGATCCATAGCTAAAGA
CTATGAAATTTCCGACGATGGTTTAGAATACACTTTTTATCTTAGAGAAGGATGGAAATTTCACGATGGAGTAGAAGTAA
CTGCGGAAGATGTTGCATTTACCCTTGAAGTAACTGCTCATCCAGATTACGATGGGCCACGTGCCAGCTGGTCAGATAAT
ATTGTAGGTGTTGATGAATACAGAGCAGGAGAAACTGATGAATTAGAAGGTGTTATTGTTGAAGATGATTATACTCTCAC
TGTAAAATCCCAAGAACCCGATGCCGGTGATATATTTGATTACTCAACTTATGCTCTTCCAAAGCATTATTATGAATTTG
ATGACTACGAAGAAATTCACGATTTAACAAATGATCCTATGGGAAGTGGACCTTTTCAATTAGTAGAGTATAGCCCGGAT
GAACACGCTATTTTAGAACCTTTCGAGGATTATTATCATGGTGAACCCGAGTTAGATCGTATAATTTACGAAGAAATTGA
AACGGAACAGCAGATCCCTATGGTTGAAACGGCAGAAGCAGATATTGTGGCAGTATCATCAACTCCCGAAAATTATGAGA
TGCTACAAGAAGAAGATCATCAAGAAACAATTACTTTCTTAGATAACTCTTATTCCTATATCGGACTTAACCATCAAAAT
GAACATTTACAACATCAAGAAGTCAGGCAAGCCTTGGCTTATGCCATAGATATTGAATCATTCATTGAAGGAATGTACGG
GGAAGAACTGGCAAGACCTATGGCAACGCCATTTTCACCTGTATCTTGGGCTTATCCTAATGAAGACCAATTAAATTTTT
ATGAATATGATCCGGATAAAGCCAATGAATTGCTTGAGGAAGCAGGATATGAATGGGATGAAAATGAAGAATATCGCTAC
AATGAAGATGGTGAACGCTTAAGTATTGTGTGGGAAACCTTAGCAGATAATGAATGGTCTGAGCATTTAACAACCCTTGC
CTTAGAACAGTGGCCACAAATAGGTGTGGATTTGGAACTAGAGTCGTATGAATTTAATACTTTAGTGGATAGAGTAAATG
TAGAAAAACGTGGAGAAGTAGATATGTGGAATATGGCATGGAGTTTAGCTACTGACCCAGATCCTAGCAATATATTCAGT
GTTCAATATGCAGATGACGGATGGAATATGGGCTACTATCACAATGAAGAGGCAGAAGAGTTAATGGAGGAAGGGATTAG
AACCTTTGATCAGGATGATCGAGCAGAAGTATATAATGAATTAGCTTTATTATTAAATAAAGATTTACCATATATCTTCG
TTTACAGCTCCAAAGATTTATGGTCTGTTAACAATAGAGTAGAAAACTTCGAACCTTCAGCTTGGCAAGCATTTTCGTGG
AACATTCATGAATGGGAGATTACCGAATATCAAGAATAG

Upstream 100 bases:

>100_bases
AAGGGAGGTGATAGTAAAATATCTGATAAATTCATTATTGAACATGATTGTAGTCTTGAAAGGAGTGGGTTAGATTATTT
TAGGAGGGGGAAACCTTATA

Downstream 100 bases:

>100_bases
GCAATGCAATGTCACCACCACTGACTTACAAATCAAATAAGAGTTCCAAGATAATTAGGGGTGCATTAAGCACCCCTAAC
TTTATACAGAAATTAGGAGG

Product: extracellular solute-binding protein family 5

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 572; Mature: 571

Protein sequence:

>572_residues
MSSRKIFVLFIAFLLTMGVALSACAPPEEAKDEEVEEKEDEEPVVENPAVERPNELIIGGTDLDGIFNPVLYSTAYDAWV
IGMMYDTLLTVDENGELTTDQRSIAKDYEISDDGLEYTFYLREGWKFHDGVEVTAEDVAFTLEVTAHPDYDGPRASWSDN
IVGVDEYRAGETDELEGVIVEDDYTLTVKSQEPDAGDIFDYSTYALPKHYYEFDDYEEIHDLTNDPMGSGPFQLVEYSPD
EHAILEPFEDYYHGEPELDRIIYEEIETEQQIPMVETAEADIVAVSSTPENYEMLQEEDHQETITFLDNSYSYIGLNHQN
EHLQHQEVRQALAYAIDIESFIEGMYGEELARPMATPFSPVSWAYPNEDQLNFYEYDPDKANELLEEAGYEWDENEEYRY
NEDGERLSIVWETLADNEWSEHLTTLALEQWPQIGVDLELESYEFNTLVDRVNVEKRGEVDMWNMAWSLATDPDPSNIFS
VQYADDGWNMGYYHNEEAEELMEEGIRTFDQDDRAEVYNELALLLNKDLPYIFVYSSKDLWSVNNRVENFEPSAWQAFSW
NIHEWEITEYQE

Sequences:

>Translated_572_residues
MSSRKIFVLFIAFLLTMGVALSACAPPEEAKDEEVEEKEDEEPVVENPAVERPNELIIGGTDLDGIFNPVLYSTAYDAWV
IGMMYDTLLTVDENGELTTDQRSIAKDYEISDDGLEYTFYLREGWKFHDGVEVTAEDVAFTLEVTAHPDYDGPRASWSDN
IVGVDEYRAGETDELEGVIVEDDYTLTVKSQEPDAGDIFDYSTYALPKHYYEFDDYEEIHDLTNDPMGSGPFQLVEYSPD
EHAILEPFEDYYHGEPELDRIIYEEIETEQQIPMVETAEADIVAVSSTPENYEMLQEEDHQETITFLDNSYSYIGLNHQN
EHLQHQEVRQALAYAIDIESFIEGMYGEELARPMATPFSPVSWAYPNEDQLNFYEYDPDKANELLEEAGYEWDENEEYRY
NEDGERLSIVWETLADNEWSEHLTTLALEQWPQIGVDLELESYEFNTLVDRVNVEKRGEVDMWNMAWSLATDPDPSNIFS
VQYADDGWNMGYYHNEEAEELMEEGIRTFDQDDRAEVYNELALLLNKDLPYIFVYSSKDLWSVNNRVENFEPSAWQAFSW
NIHEWEITEYQE
>Mature_571_residues
SSRKIFVLFIAFLLTMGVALSACAPPEEAKDEEVEEKEDEEPVVENPAVERPNELIIGGTDLDGIFNPVLYSTAYDAWVI
GMMYDTLLTVDENGELTTDQRSIAKDYEISDDGLEYTFYLREGWKFHDGVEVTAEDVAFTLEVTAHPDYDGPRASWSDNI
VGVDEYRAGETDELEGVIVEDDYTLTVKSQEPDAGDIFDYSTYALPKHYYEFDDYEEIHDLTNDPMGSGPFQLVEYSPDE
HAILEPFEDYYHGEPELDRIIYEEIETEQQIPMVETAEADIVAVSSTPENYEMLQEEDHQETITFLDNSYSYIGLNHQNE
HLQHQEVRQALAYAIDIESFIEGMYGEELARPMATPFSPVSWAYPNEDQLNFYEYDPDKANELLEEAGYEWDENEEYRYN
EDGERLSIVWETLADNEWSEHLTTLALEQWPQIGVDLELESYEFNTLVDRVNVEKRGEVDMWNMAWSLATDPDPSNIFSV
QYADDGWNMGYYHNEEAEELMEEGIRTFDQDDRAEVYNELALLLNKDLPYIFVYSSKDLWSVNNRVENFEPSAWQAFSWN
IHEWEITEYQE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=511, Percent_Identity=24.6575342465753, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI1787052, Length=468, Percent_Identity=23.9316239316239, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI1787551, Length=341, Percent_Identity=24.9266862170088, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789887, Length=496, Percent_Identity=22.9838709677419, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081878, Length=480, Percent_Identity=22.5, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 66394; Mature: 66263

Theoretical pI: Translated: 3.64; Mature: 3.64

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRKIFVLFIAFLLTMGVALSACAPPEEAKDEEVEEKEDEEPVVENPAVERPNELIIGG
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEC
TDLDGIFNPVLYSTAYDAWVIGMMYDTLLTVDENGELTTDQRSIAKDYEISDDGLEYTFY
CCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHCCCCCCCCCEEEEE
LREGWKFHDGVEVTAEDVAFTLEVTAHPDYDGPRASWSDNIVGVDEYRAGETDELEGVIV
EECCCEECCCCEEEECCEEEEEEEEECCCCCCCCCCCCCCEEECHHCCCCCCCCCCEEEE
EDDYTLTVKSQEPDAGDIFDYSTYALPKHYYEFDDYEEIHDLTNDPMGSGPFQLVEYSPD
ECCEEEEEECCCCCCCCCCCCCHHCCCHHHCCCCCHHHHHHHCCCCCCCCCEEEEEECCC
EHAILEPFEDYYHGEPELDRIIYEEIETEQQIPMVETAEADIVAVSSTPENYEMLQEEDH
CCCHHCCHHHHCCCCCHHHHHHHHHHCCHHCCCCEEECCCCEEEEECCCCHHHHHHHCCC
QETITFLDNSYSYIGLNHQNEHLQHQEVRQALAYAIDIESFIEGMYGEELARPMATPFSP
HHEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VSWAYPNEDQLNFYEYDPDKANELLEEAGYEWDENEEYRYNEDGERLSIVWETLADNEWS
CCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEHHCCCCHH
EHLTTLALEQWPQIGVDLELESYEFNTLVDRVNVEKRGEVDMWNMAWSLATDPDPSNIFS
HHHHHHHHHHCCCCCCEEEECCCCHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCCEEE
VQYADDGWNMGYYHNEEAEELMEEGIRTFDQDDRAEVYNELALLLNKDLPYIFVYSSKDL
EEECCCCCCCCCEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCE
WSVNNRVENFEPSAWQAFSWNIHEWEITEYQE
ECCCCHHCCCCCCCEEEEECCCEEEEECCCCC
>Mature Secondary Structure 
SSRKIFVLFIAFLLTMGVALSACAPPEEAKDEEVEEKEDEEPVVENPAVERPNELIIGG
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEEC
TDLDGIFNPVLYSTAYDAWVIGMMYDTLLTVDENGELTTDQRSIAKDYEISDDGLEYTFY
CCCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCHHHHHHCCCCCCCCCEEEEE
LREGWKFHDGVEVTAEDVAFTLEVTAHPDYDGPRASWSDNIVGVDEYRAGETDELEGVIV
EECCCEECCCCEEEECCEEEEEEEEECCCCCCCCCCCCCCEEECHHCCCCCCCCCCEEEE
EDDYTLTVKSQEPDAGDIFDYSTYALPKHYYEFDDYEEIHDLTNDPMGSGPFQLVEYSPD
ECCEEEEEECCCCCCCCCCCCCHHCCCHHHCCCCCHHHHHHHCCCCCCCCCEEEEEECCC
EHAILEPFEDYYHGEPELDRIIYEEIETEQQIPMVETAEADIVAVSSTPENYEMLQEEDH
CCCHHCCHHHHCCCCCHHHHHHHHHHCCHHCCCCEEECCCCEEEEECCCCHHHHHHHCCC
QETITFLDNSYSYIGLNHQNEHLQHQEVRQALAYAIDIESFIEGMYGEELARPMATPFSP
HHEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VSWAYPNEDQLNFYEYDPDKANELLEEAGYEWDENEEYRYNEDGERLSIVWETLADNEWS
CCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEEHHCCCCHH
EHLTTLALEQWPQIGVDLELESYEFNTLVDRVNVEKRGEVDMWNMAWSLATDPDPSNIFS
HHHHHHHHHHCCCCCCEEEECCCCHHHHHHHCCCCCCCCEEEEEEEEEECCCCCCCCEEE
VQYADDGWNMGYYHNEEAEELMEEGIRTFDQDDRAEVYNELALLLNKDLPYIFVYSSKDL
EEECCCCCCCCCEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCE
WSVNNRVENFEPSAWQAFSWNIHEWEITEYQE
ECCCCHHCCCCCCCEEEEECCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]