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The map label for this gene is rocR [H]

Identifier: 188585791

GI number: 188585791

Start: 1233359

End: 1234792

Strand: Direct

Name: rocR [H]

Synonym: Nther_1165

Alternate gene names: 188585791

Gene position: 1233359-1234792 (Clockwise)

Preceding gene: 188585790

Following gene: 188585792

Centisome position: 38.96

GC content: 35.36

Gene sequence:

>1434_bases
TTGAAAGCACCAGATGTATTATACAAAGATGTACTTGATGCTTTAGATGAAGGTGTACACGTGGTCGATGCCAATGGTCA
AACAATCTTTTATAACAGGCAAATGGGACAGTTAGAGGGAATGGAGCAAGAACAAGTAATTGGGAAGCCTTTATTAGAAG
TTTTTCCTTGTTTAAACTCAGAAACCAGTACTCTCTTGAAAGTATTAGAAACTGGTCTTCCAATTAAAAACAGAGTTCAA
ACTTATTTAACAATAACAGGTACTCAAGTGACTACAGTAAATAGCAGTTATCCCATTAAAATAAATGATAATATCGTTGC
AGCTTGTGAAATTGCAAAGGATATTACAGAGCTGAAAAATATGTCGGAAGAAATTATCGAACTGAAACAAAAGATGATTT
CAGAAGGAACAGAAGATTTGGATGAAGAAGATGATGAACAAACTGCTATTGTAGGTAAAAGCCGTGTGATCAGACAAGTT
CTTGATTACACTAGAAAAATAGCCAATACACCTTCCAATGTATTGATTTATGGTGAAACAGGAACAGGTAAAGAGATGTT
TGCCAGAGAGATCCATCAAAAGAGCCACAGGAAATCCAATCCTTTTATTGCTCAAAATTGTGCAGCTTTACCTTCTGAAT
TAGTCGAAGGAATTTTATTTGGAACAGTAAAGGGAGGTTTTACTGGTGCTGTAAATAGACCAGGATTATTTCAGCAAGCT
CACAAAGGGACTTTGCTTTTAGATGAAATTCATACTTTGCCACTTCATTTGCAGGCAAAGTTGTTAAGGGTTTTGGAAGA
AAAGAAAGTTCGACCAGTAGGAGGGAAAGAAGAACAGGATGTTGATGTAAGAGTTATTACAACAATGAATACTGATCCCA
TCAAGGCCATGGAAGAAGAGACCCTTCGACCAGATCTATATTATAGAATCAGTGTCATTAACTTGTTTTTGCCTCCTTTG
AGAGACAGAAAAGAAGATATCTTGCCATTGATAGACTATTTCCTACAAAAATTTCAAAAGGAATTTAATAAAGAAATTAA
AGGGATGGACCAAAACTTACTGGAAAAATTACAAAATTATTACTGGCCAGGTAATGTCAGAGAGCTAGAACACTCTATTG
AAGCAGCTATGAATGTAATTGAAAGTGGGGAACGCATGACCGTAGAACACTTGCCACCTTTAATGAAACAAAATATCTTT
GAAATTGATCAGGACTGGCACGATGATACAACAATTAATGAACAAGAACTAATTGATTTTGAAGAAGGAGAAACTCTACC
CAATAAGATGGCAAGCTTTGAAAGAAAGGCCATTGAAGGACAACTAAAAAAATATAATGGTAACATATCTCAAGCTGCTA
ATAGCTTGGGGATAAGTAGACAAGCGTTACAGTATAAAATTAAAAAGTACCGTTTAGCGGATATCGCAAATTAA

Upstream 100 bases:

>100_bases
TCTTATGTTGAATATTGATATAGAGATGACTAAACTATATTCCCTGTTTGGTGTAATAATTATATTTTCCATATTAATAT
AGGGTACTGGAGGTGAAGAT

Downstream 100 bases:

>100_bases
TTTGCACCAAACAGTAAAAATTTGCGTTAAAATCCTGTGATAACAAGGTTATATCCCTATTTATAAATTGGCATGAATTT
TGCATATAATTTAACATGAA

Product: PAS modulated sigma54 specific transcriptional regulator, Fis family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MKAPDVLYKDVLDALDEGVHVVDANGQTIFYNRQMGQLEGMEQEQVIGKPLLEVFPCLNSETSTLLKVLETGLPIKNRVQ
TYLTITGTQVTTVNSSYPIKINDNIVAACEIAKDITELKNMSEEIIELKQKMISEGTEDLDEEDDEQTAIVGKSRVIRQV
LDYTRKIANTPSNVLIYGETGTGKEMFAREIHQKSHRKSNPFIAQNCAALPSELVEGILFGTVKGGFTGAVNRPGLFQQA
HKGTLLLDEIHTLPLHLQAKLLRVLEEKKVRPVGGKEEQDVDVRVITTMNTDPIKAMEEETLRPDLYYRISVINLFLPPL
RDRKEDILPLIDYFLQKFQKEFNKEIKGMDQNLLEKLQNYYWPGNVRELEHSIEAAMNVIESGERMTVEHLPPLMKQNIF
EIDQDWHDDTTINEQELIDFEEGETLPNKMASFERKAIEGQLKKYNGNISQAANSLGISRQALQYKIKKYRLADIAN

Sequences:

>Translated_477_residues
MKAPDVLYKDVLDALDEGVHVVDANGQTIFYNRQMGQLEGMEQEQVIGKPLLEVFPCLNSETSTLLKVLETGLPIKNRVQ
TYLTITGTQVTTVNSSYPIKINDNIVAACEIAKDITELKNMSEEIIELKQKMISEGTEDLDEEDDEQTAIVGKSRVIRQV
LDYTRKIANTPSNVLIYGETGTGKEMFAREIHQKSHRKSNPFIAQNCAALPSELVEGILFGTVKGGFTGAVNRPGLFQQA
HKGTLLLDEIHTLPLHLQAKLLRVLEEKKVRPVGGKEEQDVDVRVITTMNTDPIKAMEEETLRPDLYYRISVINLFLPPL
RDRKEDILPLIDYFLQKFQKEFNKEIKGMDQNLLEKLQNYYWPGNVRELEHSIEAAMNVIESGERMTVEHLPPLMKQNIF
EIDQDWHDDTTINEQELIDFEEGETLPNKMASFERKAIEGQLKKYNGNISQAANSLGISRQALQYKIKKYRLADIAN
>Mature_477_residues
MKAPDVLYKDVLDALDEGVHVVDANGQTIFYNRQMGQLEGMEQEQVIGKPLLEVFPCLNSETSTLLKVLETGLPIKNRVQ
TYLTITGTQVTTVNSSYPIKINDNIVAACEIAKDITELKNMSEEIIELKQKMISEGTEDLDEEDDEQTAIVGKSRVIRQV
LDYTRKIANTPSNVLIYGETGTGKEMFAREIHQKSHRKSNPFIAQNCAALPSELVEGILFGTVKGGFTGAVNRPGLFQQA
HKGTLLLDEIHTLPLHLQAKLLRVLEEKKVRPVGGKEEQDVDVRVITTMNTDPIKAMEEETLRPDLYYRISVINLFLPPL
RDRKEDILPLIDYFLQKFQKEFNKEIKGMDQNLLEKLQNYYWPGNVRELEHSIEAAMNVIESGERMTVEHLPPLMKQNIF
EIDQDWHDDTTINEQELIDFEEGETLPNKMASFERKAIEGQLKKYNGNISQAANSLGISRQALQYKIKKYRLADIAN

Specific function: Positive regulator of arginine catabolism. Controls the transcription of the two operons rocABC and rocDEF and probably acts by binding to the corresponding upstream activating sequences [H]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=362, Percent_Identity=41.4364640883978, Blast_Score=251, Evalue=5e-68,
Organism=Escherichia coli, GI1789233, Length=469, Percent_Identity=34.9680170575693, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI1788905, Length=325, Percent_Identity=38.7692307692308, Blast_Score=213, Evalue=3e-56,
Organism=Escherichia coli, GI87082117, Length=333, Percent_Identity=39.3393393393393, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI1790437, Length=338, Percent_Identity=36.6863905325444, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1789087, Length=259, Percent_Identity=43.2432432432432, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI1790299, Length=329, Percent_Identity=36.1702127659575, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI1786524, Length=336, Percent_Identity=37.202380952381, Blast_Score=191, Evalue=9e-50,
Organism=Escherichia coli, GI87082152, Length=290, Percent_Identity=40.3448275862069, Blast_Score=189, Evalue=3e-49,
Organism=Escherichia coli, GI1787583, Length=330, Percent_Identity=35.7575757575758, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI87081872, Length=230, Percent_Identity=40.4347826086956, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI87081858, Length=475, Percent_Identity=25.4736842105263, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1789828, Length=226, Percent_Identity=36.7256637168142, Blast_Score=125, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR000014
- InterPro:   IPR013656
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF08448 PAS_4; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 54218; Mature: 54218

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS50112 PAS ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAPDVLYKDVLDALDEGVHVVDANGQTIFYNRQMGQLEGMEQEQVIGKPLLEVFPCLNS
CCCCHHHHHHHHHHHHCCCEEEECCCCEEEEECCCHHHCCCCHHHHHHHHHHHHHHCCCC
ETSTLLKVLETGLPIKNRVQTYLTITGTQVTTVNSSYPIKINDNIVAACEIAKDITELKN
HHHHHHHHHHHCCCHHHCCEEEEEEECCEEEEECCCCCEEECCCEEEHHHHHHHHHHHHH
MSEEIIELKQKMISEGTEDLDEEDDEQTAIVGKSRVIRQVLDYTRKIANTPSNVLIYGET
HHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCC
GTGKEMFAREIHQKSHRKSNPFIAQNCAALPSELVEGILFGTVKGGFTGAVNRPGLFQQA
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
HKGTLLLDEIHTLPLHLQAKLLRVLEEKKVRPVGGKEEQDVDVRVITTMNTDPIKAMEEE
CCCCEEEHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHH
TLRPDLYYRISVINLFLPPLRDRKEDILPLIDYFLQKFQKEFNKEIKGMDQNLLEKLQNY
CCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
YWPGNVRELEHSIEAAMNVIESGERMTVEHLPPLMKQNIFEIDQDWHDDTTINEQELIDF
CCCCCHHHHHHHHHHHHHHHHCCCEEEHHHCCHHHHHHHHHHCCCCCCCCCCCHHHCCCC
EEGETLPNKMASFERKAIEGQLKKYNGNISQAANSLGISRQALQYKIKKYRLADIAN
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKAPDVLYKDVLDALDEGVHVVDANGQTIFYNRQMGQLEGMEQEQVIGKPLLEVFPCLNS
CCCCHHHHHHHHHHHHCCCEEEECCCCEEEEECCCHHHCCCCHHHHHHHHHHHHHHCCCC
ETSTLLKVLETGLPIKNRVQTYLTITGTQVTTVNSSYPIKINDNIVAACEIAKDITELKN
HHHHHHHHHHHCCCHHHCCEEEEEEECCEEEEECCCCCEEECCCEEEHHHHHHHHHHHHH
MSEEIIELKQKMISEGTEDLDEEDDEQTAIVGKSRVIRQVLDYTRKIANTPSNVLIYGET
HHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCC
GTGKEMFAREIHQKSHRKSNPFIAQNCAALPSELVEGILFGTVKGGFTGAVNRPGLFQQA
CCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
HKGTLLLDEIHTLPLHLQAKLLRVLEEKKVRPVGGKEEQDVDVRVITTMNTDPIKAMEEE
CCCCEEEHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHH
TLRPDLYYRISVINLFLPPLRDRKEDILPLIDYFLQKFQKEFNKEIKGMDQNLLEKLQNY
CCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
YWPGNVRELEHSIEAAMNVIESGERMTVEHLPPLMKQNIFEIDQDWHDDTTINEQELIDF
CCCCCHHHHHHHHHHHHHHHHCCCEEEHHHCCHHHHHHHHHHCCCCCCCCCCCHHHCCCC
EEGETLPNKMASFERKAIEGQLKKYNGNISQAANSLGISRQALQYKIKKYRLADIAN
CCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8113162; 9384377 [H]