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The map label for this gene is hemB [H]

Identifier: 188585753

GI number: 188585753

Start: 1194998

End: 1195978

Strand: Direct

Name: hemB [H]

Synonym: Nther_1127

Alternate gene names: 188585753

Gene position: 1194998-1195978 (Clockwise)

Preceding gene: 188585752

Following gene: 188585754

Centisome position: 37.75

GC content: 40.37

Gene sequence:

>981_bases
ATGAAATATCCTGAGTATCGAATGAGGCGATTAAGAGCGACTAAACAAATGAGACAGATGGTTCGAGAGCATAAATTGGA
TGTAAGTGATTTGATTTACCCTGTTTTTGTAAATAGTGCCATCCAGGAGCCCAAACCAGTGCCCTCAATGCCTGGTGTTT
ATCAATGGCCTGTAGATAAAATTGTACAACATCTAAAAGAAATTTATGAATTAGGCATACCAGCGATAATGTTATTTGGT
ATACCTAATTATAAAGACTCAACAGGTAGTTCGGGAAAAGATTCAGAAGAAGCTGTTCAAAGAGCTTGCCGTGAAATCAA
AAGTAGTTTACCAGAACTGACTGTAATTACTGATGTTTGCCTGTGTGGCTATACTACCCATGGGCATTGTGGTTTAATAA
AAGAGGGAACCGTTGATAATGATTCTACTATAGAAGAACTAGCTGAAATTGCTATTTCTCATGGACAAGCAGGGGCCGAT
ATGGTGGCTCCTTCCAATATGATGGATGGTTTTGTAAAAGTGATTCGGGACAAACTAGATGAGGCAGGCTTTTCACAGTT
ACCTATCATGGCTTATTCAGCGAAATTTTCATCTAGCTTTTATGGACCTTTTAGAGACGCTGCTGAATCAGCTCCTTCAG
AAGGTGATCGCAGATCCTATCAAATGGACCCTGCCAATGGTAAAGAAGCACTCAGAGAAGTTGAGCTTGATATTAACGAA
GGTGCCGATATAGTTATGATTAAACCCGGTTTAAGTTATCTGGATGTGATAAAAGAAGTGAAACAAAAGTTCAACCATCC
AATAGCAGCTTACAATGTCAGTGGTGAGTATTCCATGATAAAAGCAGCTGCTGACAAGGGCTGGCTAAATGAGCGGGAAG
TAGTTCTGGAAAAGTTGATTAGTTTCAAGCGAGCTGGAGCTGATATGATCCTGACTTACTTTGCCGATGATGCAGCCCGC
TGGCTTCAAGGAATTGAGTAA

Upstream 100 bases:

>100_bases
ACTGATAAAGAGATCGGTTTAAAATAATTTTTTATACAACGTGATATAACAACTTTTGAAGGGAAAAGTTTTAATAAATA
GCTACTAGGGGGTGGCAGTA

Downstream 100 bases:

>100_bases
TAGCTATAGGAGGCGATTAGCAGATGCTAGTGTCGTGGAATACAACCAAAAGTTGTCACTTAAATTGTAAGCACTGTTAT
CGTGATGCCGGGGAAGCTGA

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MKYPEYRMRRLRATKQMRQMVREHKLDVSDLIYPVFVNSAIQEPKPVPSMPGVYQWPVDKIVQHLKEIYELGIPAIMLFG
IPNYKDSTGSSGKDSEEAVQRACREIKSSLPELTVITDVCLCGYTTHGHCGLIKEGTVDNDSTIEELAEIAISHGQAGAD
MVAPSNMMDGFVKVIRDKLDEAGFSQLPIMAYSAKFSSSFYGPFRDAAESAPSEGDRRSYQMDPANGKEALREVELDINE
GADIVMIKPGLSYLDVIKEVKQKFNHPIAAYNVSGEYSMIKAAADKGWLNEREVVLEKLISFKRAGADMILTYFADDAAR
WLQGIE

Sequences:

>Translated_326_residues
MKYPEYRMRRLRATKQMRQMVREHKLDVSDLIYPVFVNSAIQEPKPVPSMPGVYQWPVDKIVQHLKEIYELGIPAIMLFG
IPNYKDSTGSSGKDSEEAVQRACREIKSSLPELTVITDVCLCGYTTHGHCGLIKEGTVDNDSTIEELAEIAISHGQAGAD
MVAPSNMMDGFVKVIRDKLDEAGFSQLPIMAYSAKFSSSFYGPFRDAAESAPSEGDRRSYQMDPANGKEALREVELDINE
GADIVMIKPGLSYLDVIKEVKQKFNHPIAAYNVSGEYSMIKAAADKGWLNEREVVLEKLISFKRAGADMILTYFADDAAR
WLQGIE
>Mature_326_residues
MKYPEYRMRRLRATKQMRQMVREHKLDVSDLIYPVFVNSAIQEPKPVPSMPGVYQWPVDKIVQHLKEIYELGIPAIMLFG
IPNYKDSTGSSGKDSEEAVQRACREIKSSLPELTVITDVCLCGYTTHGHCGLIKEGTVDNDSTIEELAEIAISHGQAGAD
MVAPSNMMDGFVKVIRDKLDEAGFSQLPIMAYSAKFSSSFYGPFRDAAESAPSEGDRRSYQMDPANGKEALREVELDINE
GADIVMIKPGLSYLDVIKEVKQKFNHPIAAYNVSGEYSMIKAAADKGWLNEREVVLEKLISFKRAGADMILTYFADDAAR
WLQGIE

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=303, Percent_Identity=43.2343234323432, Blast_Score=276, Evalue=2e-74,
Organism=Escherichia coli, GI87081728, Length=313, Percent_Identity=49.2012779552716, Blast_Score=302, Evalue=2e-83,
Organism=Saccharomyces cerevisiae, GI6321398, Length=312, Percent_Identity=39.7435897435897, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI21358291, Length=288, Percent_Identity=41.3194444444444, Blast_Score=263, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36403; Mature: 36403

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYPEYRMRRLRATKQMRQMVREHKLDVSDLIYPVFVNSAIQEPKPVPSMPGVYQWPVDK
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
IVQHLKEIYELGIPAIMLFGIPNYKDSTGSSGKDSEEAVQRACREIKSSLPELTVITDVC
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
LCGYTTHGHCGLIKEGTVDNDSTIEELAEIAISHGQAGADMVAPSNMMDGFVKVIRDKLD
HHCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
EAGFSQLPIMAYSAKFSSSFYGPFRDAAESAPSEGDRRSYQMDPANGKEALREVELDINE
HCCCCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
GADIVMIKPGLSYLDVIKEVKQKFNHPIAAYNVSGEYSMIKAAADKGWLNEREVVLEKLI
CCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHH
SFKRAGADMILTYFADDAARWLQGIE
HHHHCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKYPEYRMRRLRATKQMRQMVREHKLDVSDLIYPVFVNSAIQEPKPVPSMPGVYQWPVDK
CCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
IVQHLKEIYELGIPAIMLFGIPNYKDSTGSSGKDSEEAVQRACREIKSSLPELTVITDVC
HHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHH
LCGYTTHGHCGLIKEGTVDNDSTIEELAEIAISHGQAGADMVAPSNMMDGFVKVIRDKLD
HHCCCCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHH
EAGFSQLPIMAYSAKFSSSFYGPFRDAAESAPSEGDRRSYQMDPANGKEALREVELDINE
HCCCCCCCHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
GADIVMIKPGLSYLDVIKEVKQKFNHPIAAYNVSGEYSMIKAAADKGWLNEREVVLEKLI
CCCEEEECCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHH
SFKRAGADMILTYFADDAARWLQGIE
HHHHCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8123787 [H]