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The map label for this gene is rnfB [H]

Identifier: 188585713

GI number: 188585713

Start: 1145160

End: 1146020

Strand: Direct

Name: rnfB [H]

Synonym: Nther_1086

Alternate gene names: 188585713

Gene position: 1145160-1146020 (Clockwise)

Preceding gene: 188585712

Following gene: 188585714

Centisome position: 36.18

GC content: 40.88

Gene sequence:

>861_bases
ATGATTGAAGCTGTTTTAAGTTTAGGAGCTATTGCGTTTATTTTTGGATCTGTATTAGCTTATGCCGCGGAAAAATTTAA
AGTCGAGAAAGACCCACGCATAGAGGCAATTGCTGAAGTTGTGCCGGGTGCTAATTGCGGTGCCTGTGGGTATCCAGGTT
GTGAAGCTTTTGCAGAAGCTGTAGCCAGGGGAGAAGCTCCACCTGAAGGTTGTACACCTGGAGGAAAGAAGGCAGCCGAA
CAGATTTCAGAGATACTGTCTAATGGAGCAGGTGAGAGCAATGAGTTTAGCACCTTGGGTTATGTGAAAGAAACCAATAA
AGTGATACAGGAACCTTCTCAAAAATACATTGCAGAAGTGGCTTGCTTAGGCTCTAGAGAGTATTGTAAGGAGAAGTTTG
AGTATGATGGAGTCAGAGATTGCCGGGCTGCAATGATGTACCGAAATGGTCCAAAAGCTTGTGAATTTGGGTGTATAGGC
CTAGCTACCTGTGTTGAAACATGTCCTTTTGAAGCTATTGAAATGAGAGATGACGGTCTTCCTCGTATAAACCATGATAT
ATGCAGGGGCTGTGCCACTTGTGTAAATACATGTCCTAAGAGTGTCATAAGATTGATACCGGGAGATAGGTACAAACATT
TTATATATTGTAACTCTCAAGAAACGGGAGAAATTGTAAAGGAAAACTGTCAATATGGATGTCTTGGTTGTGGTATTTGC
CGTGAACAATGTCCCGTTGGTGCTATAAATTTAGATAATGATTTAGCAGTTATTGATCAAAATAAGTGTGTTAACTGTGG
TTTGTGTAAAGATAAATGCCCAACAGCTTGCATTGTAAGTGAATCTGAAAGACTTCAATAA

Upstream 100 bases:

>100_bases
CTTTTATTACCGCGGGAATTCTTGCCCTGGCTTTTTCAGGATTTTTAGGCATAATCTAGTGAATAATTTTAAGGATGTGA
TACAAAAGGAGTGTAATTAG

Downstream 100 bases:

>100_bases
AAAAACGCAAAATCTGAAATTCCAAGTCAAAATCCCATAGTATGAAAACCACTTTTAATCTAGGAGGGAGAATATGAAAA
AGACTAAACTGTATCGTAGA

Product: electron transport complex, RnfABCDGE type, B subunit

Products: NA

Alternate protein names: Nitrogen fixation protein rnfB [H]

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MIEAVLSLGAIAFIFGSVLAYAAEKFKVEKDPRIEAIAEVVPGANCGACGYPGCEAFAEAVARGEAPPEGCTPGGKKAAE
QISEILSNGAGESNEFSTLGYVKETNKVIQEPSQKYIAEVACLGSREYCKEKFEYDGVRDCRAAMMYRNGPKACEFGCIG
LATCVETCPFEAIEMRDDGLPRINHDICRGCATCVNTCPKSVIRLIPGDRYKHFIYCNSQETGEIVKENCQYGCLGCGIC
REQCPVGAINLDNDLAVIDQNKCVNCGLCKDKCPTACIVSESERLQ

Sequences:

>Translated_286_residues
MIEAVLSLGAIAFIFGSVLAYAAEKFKVEKDPRIEAIAEVVPGANCGACGYPGCEAFAEAVARGEAPPEGCTPGGKKAAE
QISEILSNGAGESNEFSTLGYVKETNKVIQEPSQKYIAEVACLGSREYCKEKFEYDGVRDCRAAMMYRNGPKACEFGCIG
LATCVETCPFEAIEMRDDGLPRINHDICRGCATCVNTCPKSVIRLIPGDRYKHFIYCNSQETGEIVKENCQYGCLGCGIC
REQCPVGAINLDNDLAVIDQNKCVNCGLCKDKCPTACIVSESERLQ
>Mature_286_residues
MIEAVLSLGAIAFIFGSVLAYAAEKFKVEKDPRIEAIAEVVPGANCGACGYPGCEAFAEAVARGEAPPEGCTPGGKKAAE
QISEILSNGAGESNEFSTLGYVKETNKVIQEPSQKYIAEVACLGSREYCKEKFEYDGVRDCRAAMMYRNGPKACEFGCIG
LATCVETCPFEAIEMRDDGLPRINHDICRGCATCVNTCPKSVIRLIPGDRYKHFIYCNSQETGEIVKENCQYGCLGCGIC
REQCPVGAINLDNDLAVIDQNKCVNCGLCKDKCPTACIVSESERLQ

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase [H]

COG id: COG2878

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfB

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787915, Length=208, Percent_Identity=27.8846153846154, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010207
- InterPro:   IPR016463
- InterPro:   IPR007202
- InterPro:   IPR012285 [H]

Pfam domain/function: PF00037 Fer4; PF04060 FeS [H]

EC number: NA

Molecular weight: Translated: 30845; Mature: 30845

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

9.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
10.5 %Cys+Met (Translated Protein)
9.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
10.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEAVLSLGAIAFIFGSVLAYAAEKFKVEKDPRIEAIAEVVPGANCGACGYPGCEAFAEA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHH
VARGEAPPEGCTPGGKKAAEQISEILSNGAGESNEFSTLGYVKETNKVIQEPSQKYIAEV
HHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHH
ACLGSREYCKEKFEYDGVRDCRAAMMYRNGPKACEFGCIGLATCVETCPFEAIEMRDDGL
HHCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHCCCHHEECCCCCC
PRINHDICRGCATCVNTCPKSVIRLIPGDRYKHFIYCNSQETGEIVKENCQYGCLGCGIC
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHCCCCCCCCHHHH
REQCPVGAINLDNDLAVIDQNKCVNCGLCKDKCPTACIVSESERLQ
HHHCCCCEEECCCCEEEEECCCCCCCCCCCCCCCCEEEECCHHCCC
>Mature Secondary Structure
MIEAVLSLGAIAFIFGSVLAYAAEKFKVEKDPRIEAIAEVVPGANCGACGYPGCEAFAEA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHH
VARGEAPPEGCTPGGKKAAEQISEILSNGAGESNEFSTLGYVKETNKVIQEPSQKYIAEV
HHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHH
ACLGSREYCKEKFEYDGVRDCRAAMMYRNGPKACEFGCIGLATCVETCPFEAIEMRDDGL
HHCCCHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHCCHHHHHHHHHHCCCHHEECCCCCC
PRINHDICRGCATCVNTCPKSVIRLIPGDRYKHFIYCNSQETGEIVKENCQYGCLGCGIC
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHCCCCCCCCHHHH
REQCPVGAINLDNDLAVIDQNKCVNCGLCKDKCPTACIVSESERLQ
HHHCCCCEEECCCCEEEEECCCCCCCCCCCCCCCCEEEECCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA