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The map label for this gene is lysS [H]

Identifier: 188584759

GI number: 188584759

Start: 134047

End: 135516

Strand: Direct

Name: lysS [H]

Synonym: Nther_0117

Alternate gene names: 188584759

Gene position: 134047-135516 (Clockwise)

Preceding gene: 188584758

Following gene: 188584760

Centisome position: 4.23

GC content: 37.21

Gene sequence:

>1470_bases
ATGGAGAAAGATCATGAATTGATCAATGTCAGGTACGAAAAAGCCCGCGACATGGAAAATCGCAACATCCAACCTTTTGG
CGACAAATATTCTGCCACTTACTATGCTCAAGAAGTTAAAGATAACTATGAACAATTGTTAGAAACTAAAGAAGAAGTTA
GCTTATCAGGACGTATTATGGCCAAGCGAGGCCATGGCAAAGCAGGATTTGCCCATATCCAAGACTCTAGTGGACAAGTG
CAGATTTATACACGAGTTGATAATGTTGGGGAAGAAGACTACGAATTGTATAAAGAACTGGATATTGGAGATATTATTGC
TTTAAAAGGGCCAGTTTTTATGACTAGAAAAGGTGAAATTACTGTTGAGGTCAAAGAATTTCACCTCCAAAGTAAGTCTT
TACGTCCTCTACCTGAAAAATGGCATGGATTGAAAGATGTAGACCTGCGCTACCGTAAACGATACTTAGATCTGATCATG
AATCAAGATGTTAAAGATACTTTTGTAATTAGAAGTAAGATAATCCGAGAAATGCGAAGATATTTGGATGATAGAGGTTT
TTTTGAAATGGAAACCCCCACCCTACATACAATTCCAGGTGGTGCAAATGCTAGACCATTTAAAACATATCACAACGCTT
TAGAAATGAAACTTTATATGAGAATAGCAACTGAATTACACTTGAAGAGGCTAATTGTTGGTGGATTTGATAAAGTTTAC
GAATTAGGTAGAATTTTTAGGAATGAAGGAATATCAACTAAACATAATCCTGAATTTACCTCAATTGAACTATATCAATC
TAATAGTGATTACCATGACATGATGGAGCTTACTGAAAATATGATTTATGAAATCGCAAACAAAATTTTTGGAACCCCAG
TGGTTGAATATCAGGGGACAAATATTGACTTCACTCCACCATGGCCTAGAAAAACAATGATTGATGCCGTGAAAGAGGCT
ACAGGAATTGATTTTTCCGAGCTAAATCATGAAAAAGCCAGAGAAAAAGCCGAACAAGCGGGAATAGAAGTCGAGAAAGA
TGCCACATGGGGTGAAGCCTTAAACGAAATTTTTGAGGAAAAGGTAGAAGATAGTTTGGTTCAACCCACCTTCATTCTAG
ACTATCCTGTTGAAGTTTCACCTTTGGCAAAAAGGCATGAAGAAGATAGTCGCTTAACTTACAGATTTGAAGGATTCCTG
TGGGGAAGTGAAATAGCAAATGCTTTTACAGAACTTAATGACCCTAGAGATCAAAGGGAACGATTTGAAAGTCAAGTGGA
AAAACGTGATGCGGGTGACGAGGAAGCTCATATGATGGATGAAGATTTTGTTGAAGCTTTAGAATATGGTATGCCTCCCA
CAGGTGGCCTTGGGATTGGAATAGATAGATTAGTAATGTTTATGACAAACTCTACATCTATAAGAGATGTAATTTTATTC
CCTACAATGAGGCCAAAGCAGGATGAATAA

Upstream 100 bases:

>100_bases
TTGAGTCCCGGGTGATAGCCTGGGACTCATAACTTTATATTTTCTTTAATACGTTAACGAAGCCGGGGTAATAATTATAA
TAAACGAGAGGTGAACATAT

Downstream 100 bases:

>100_bases
ATCAAACTGTTAACCATTGAGTTAGATAATTTGTCAAAACGAAACTAACTTGGTAACAGTTTCACATTAAAAGATTTGCG
TTTACTCTTGAAAAAGATGA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MEKDHELINVRYEKARDMENRNIQPFGDKYSATYYAQEVKDNYEQLLETKEEVSLSGRIMAKRGHGKAGFAHIQDSSGQV
QIYTRVDNVGEEDYELYKELDIGDIIALKGPVFMTRKGEITVEVKEFHLQSKSLRPLPEKWHGLKDVDLRYRKRYLDLIM
NQDVKDTFVIRSKIIREMRRYLDDRGFFEMETPTLHTIPGGANARPFKTYHNALEMKLYMRIATELHLKRLIVGGFDKVY
ELGRIFRNEGISTKHNPEFTSIELYQSNSDYHDMMELTENMIYEIANKIFGTPVVEYQGTNIDFTPPWPRKTMIDAVKEA
TGIDFSELNHEKAREKAEQAGIEVEKDATWGEALNEIFEEKVEDSLVQPTFILDYPVEVSPLAKRHEEDSRLTYRFEGFL
WGSEIANAFTELNDPRDQRERFESQVEKRDAGDEEAHMMDEDFVEALEYGMPPTGGLGIGIDRLVMFMTNSTSIRDVILF
PTMRPKQDE

Sequences:

>Translated_489_residues
MEKDHELINVRYEKARDMENRNIQPFGDKYSATYYAQEVKDNYEQLLETKEEVSLSGRIMAKRGHGKAGFAHIQDSSGQV
QIYTRVDNVGEEDYELYKELDIGDIIALKGPVFMTRKGEITVEVKEFHLQSKSLRPLPEKWHGLKDVDLRYRKRYLDLIM
NQDVKDTFVIRSKIIREMRRYLDDRGFFEMETPTLHTIPGGANARPFKTYHNALEMKLYMRIATELHLKRLIVGGFDKVY
ELGRIFRNEGISTKHNPEFTSIELYQSNSDYHDMMELTENMIYEIANKIFGTPVVEYQGTNIDFTPPWPRKTMIDAVKEA
TGIDFSELNHEKAREKAEQAGIEVEKDATWGEALNEIFEEKVEDSLVQPTFILDYPVEVSPLAKRHEEDSRLTYRFEGFL
WGSEIANAFTELNDPRDQRERFESQVEKRDAGDEEAHMMDEDFVEALEYGMPPTGGLGIGIDRLVMFMTNSTSIRDVILF
PTMRPKQDE
>Mature_489_residues
MEKDHELINVRYEKARDMENRNIQPFGDKYSATYYAQEVKDNYEQLLETKEEVSLSGRIMAKRGHGKAGFAHIQDSSGQV
QIYTRVDNVGEEDYELYKELDIGDIIALKGPVFMTRKGEITVEVKEFHLQSKSLRPLPEKWHGLKDVDLRYRKRYLDLIM
NQDVKDTFVIRSKIIREMRRYLDDRGFFEMETPTLHTIPGGANARPFKTYHNALEMKLYMRIATELHLKRLIVGGFDKVY
ELGRIFRNEGISTKHNPEFTSIELYQSNSDYHDMMELTENMIYEIANKIFGTPVVEYQGTNIDFTPPWPRKTMIDAVKEA
TGIDFSELNHEKAREKAEQAGIEVEKDATWGEALNEIFEEKVEDSLVQPTFILDYPVEVSPLAKRHEEDSRLTYRFEGFL
WGSEIANAFTELNDPRDQRERFESQVEKRDAGDEEAHMMDEDFVEALEYGMPPTGGLGIGIDRLVMFMTNSTSIRDVILF
PTMRPKQDE

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=450, Percent_Identity=45.1111111111111, Blast_Score=389, Evalue=1e-108,
Organism=Homo sapiens, GI194272210, Length=450, Percent_Identity=45.1111111111111, Blast_Score=387, Evalue=1e-107,
Organism=Homo sapiens, GI45439306, Length=462, Percent_Identity=22.7272727272727, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI4758762, Length=345, Percent_Identity=24.3478260869565, Blast_Score=88, Evalue=2e-17,
Organism=Homo sapiens, GI40789249, Length=245, Percent_Identity=28.5714285714286, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI226491502, Length=472, Percent_Identity=21.1864406779661, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1790571, Length=453, Percent_Identity=51.2141280353201, Blast_Score=476, Evalue=1e-135,
Organism=Escherichia coli, GI1789256, Length=445, Percent_Identity=51.2359550561798, Blast_Score=467, Evalue=1e-133,
Organism=Escherichia coli, GI87082379, Length=322, Percent_Identity=32.2981366459627, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI1788173, Length=268, Percent_Identity=26.4925373134328, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17535925, Length=502, Percent_Identity=44.6215139442231, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI17535927, Length=502, Percent_Identity=44.6215139442231, Blast_Score=397, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI71994340, Length=502, Percent_Identity=44.6215139442231, Blast_Score=396, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17551876, Length=473, Percent_Identity=22.8329809725159, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI32566633, Length=244, Percent_Identity=25.8196721311475, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6320242, Length=491, Percent_Identity=42.3625254582485, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324256, Length=475, Percent_Identity=31.7894736842105, Blast_Score=181, Evalue=3e-46,
Organism=Saccharomyces cerevisiae, GI6323011, Length=348, Percent_Identity=25.5747126436782, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6321807, Length=458, Percent_Identity=24.0174672489083, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24640849, Length=483, Percent_Identity=43.2712215320911, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24640851, Length=483, Percent_Identity=43.2712215320911, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24584738, Length=227, Percent_Identity=27.7533039647577, Blast_Score=75, Evalue=8e-14,
Organism=Drosophila melanogaster, GI19921528, Length=343, Percent_Identity=22.4489795918367, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 56929; Mature: 56929

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKDHELINVRYEKARDMENRNIQPFGDKYSATYYAQEVKDNYEQLLETKEEVSLSGRIM
CCCCCCHHEEEHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
AKRGHGKAGFAHIQDSSGQVQIYTRVDNVGEEDYELYKELDIGDIIALKGPVFMTRKGEI
EECCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHCCCCCEEEECCCEEEEECCCE
TVEVKEFHLQSKSLRPLPEKWHGLKDVDLRYRKRYLDLIMNQDVKDTFVIRSKIIREMRR
EEEEHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
YLDDRGFFEMETPTLHTIPGGANARPFKTYHNALEMKLYMRIATELHLKRLIVGGFDKVY
HHCCCCCEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ELGRIFRNEGISTKHNPEFTSIELYQSNSDYHDMMELTENMIYEIANKIFGTPVVEYQGT
HHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
NIDFTPPWPRKTMIDAVKEATGIDFSELNHEKAREKAEQAGIEVEKDATWGEALNEIFEE
CCCCCCCCCHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHH
KVEDSLVQPTFILDYPVEVSPLAKRHEEDSRLTYRFEGFLWGSEIANAFTELNDPRDQRE
HHHHHHCCCEEEEECCCCCCHHHHCCCCCCCEEEEEEEEECHHHHHHHHHHCCCCHHHHH
RFESQVEKRDAGDEEAHMMDEDFVEALEYGMPPTGGLGIGIDRLVMFMTNSTSIRDVILF
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEE
PTMRPKQDE
CCCCCCCCC
>Mature Secondary Structure
MEKDHELINVRYEKARDMENRNIQPFGDKYSATYYAQEVKDNYEQLLETKEEVSLSGRIM
CCCCCCHHEEEHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
AKRGHGKAGFAHIQDSSGQVQIYTRVDNVGEEDYELYKELDIGDIIALKGPVFMTRKGEI
EECCCCCCCEEEEECCCCCEEEEEECCCCCCHHHHHHHHCCCCCEEEECCCEEEEECCCE
TVEVKEFHLQSKSLRPLPEKWHGLKDVDLRYRKRYLDLIMNQDVKDTFVIRSKIIREMRR
EEEEHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
YLDDRGFFEMETPTLHTIPGGANARPFKTYHNALEMKLYMRIATELHLKRLIVGGFDKVY
HHCCCCCEEECCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
ELGRIFRNEGISTKHNPEFTSIELYQSNSDYHDMMELTENMIYEIANKIFGTPVVEYQGT
HHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
NIDFTPPWPRKTMIDAVKEATGIDFSELNHEKAREKAEQAGIEVEKDATWGEALNEIFEE
CCCCCCCCCHHHHHHHHHHHHCCCHHHCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHH
KVEDSLVQPTFILDYPVEVSPLAKRHEEDSRLTYRFEGFLWGSEIANAFTELNDPRDQRE
HHHHHHCCCEEEEECCCCCCHHHHCCCCCCCEEEEEEEEECHHHHHHHHHHCCCCHHHHH
RFESQVEKRDAGDEEAHMMDEDFVEALEYGMPPTGGLGIGIDRLVMFMTNSTSIRDVILF
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEEEEEE
PTMRPKQDE
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA