Click here to switch to the map view.

The map label for this gene is birA [H]

Identifier: 188584727

GI number: 188584727

Start: 103637

End: 104608

Strand: Direct

Name: birA [H]

Synonym: Nther_0085

Alternate gene names: 188584727

Gene position: 103637-104608 (Clockwise)

Preceding gene: 188584725

Following gene: 188584728

Centisome position: 3.27

GC content: 36.63

Gene sequence:

>972_bases
GTGAGTATTATTAAATTATCGGAAACGGATCTCAGTCCTGTAAAATCAAAAATTTTAAACAATTTGAAAAAACAGCCATA
TGTTTCTGGTGAATTTCTAAGTTCTCAATTAAATGTTTCCAGGACAGCCATTTGGAAGAATATTCAAGGGCTGGCCAATT
TAGGTTATCAAATTGATCCCATAAAGGGTAAAGGATATCGATTGGTAGTTATACCTGATAACCTTTATCCATGGGAACTA
GATTTTTCGTTAAAAAAAGATTACCAAATAACTTACTTTGATAATATCGATTCTACTAATAATTATGCACGTTCTTTGCA
ACCACCGCAAGTGGTGATTGCTGAACAACAGACCAAAGGGCGGGGTCGTATGGGCAGGGCGTGGTTTTCTGCCCCTGGAG
GAATTTATTTTTCCTTAGTGATTACTCCACGGTGTTCAGTAGATGAGATTCCTACAATTCCCTTGCTGATATCAACTGCT
ATTACCAAAGTTCTTAAAAGGGAACTGGGAATCCAAGCTGAAATAAAATGGCCTAATGATATTTTAGTTCAAGGTCAAAA
AGTGACTGGGATCTTAGTAGAACTTGCTGGCGAAACCGATGCTCCTCAGAAAGCTGTAATAGGGGTGGGAATTAATGCCA
ACCAAAATACCAATTTATTCCCTGAGGAGCTGAAAAAGTCTGTCACTTCCTTGAGAGAACTTAATTGCTCGCCAGTAAAT
CGCAAATTAATGTTAAATAGTATATTAGAAGAGATTACTCAATTATTTTTTGAATTACCAAATTGTATCCCTAAGGTCTT
AAATACCTGGAAGGAATACTCCTGCACTTTAGGGAAAAAAATTACTGTAAAACAAGTGTCGAAGACAATTACAGGGATTG
CAAAAGATATTGATAGAAGTGGGGCTTTGATTGTGGAGACCCCCCAAGGAGAACAAAGAATACTAACCGGTGATTTATCT
ATTAATATTTAG

Upstream 100 bases:

>100_bases
TGGATTTGAATTTCTCTTTGCATTTGACTTAAAGATAGCATCGATCGTAAATATAATTGTGCCTTGTATGTAAATTTGAT
GAATCTATATAATGTAAGAG

Downstream 100 bases:

>100_bases
GGAGGAGATGTATGATGTTATTAGCAATTGATGTAGGAAACACTAATATAGTATTAGGGCTTTATAAAAATGATGAATTA
GTAGTTCACTGGCGAATAAA

Product: biotin--acetyl-CoA-carboxylase ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSIIKLSETDLSPVKSKILNNLKKQPYVSGEFLSSQLNVSRTAIWKNIQGLANLGYQIDPIKGKGYRLVVIPDNLYPWEL
DFSLKKDYQITYFDNIDSTNNYARSLQPPQVVIAEQQTKGRGRMGRAWFSAPGGIYFSLVITPRCSVDEIPTIPLLISTA
ITKVLKRELGIQAEIKWPNDILVQGQKVTGILVELAGETDAPQKAVIGVGINANQNTNLFPEELKKSVTSLRELNCSPVN
RKLMLNSILEEITQLFFELPNCIPKVLNTWKEYSCTLGKKITVKQVSKTITGIAKDIDRSGALIVETPQGEQRILTGDLS
INI

Sequences:

>Translated_323_residues
MSIIKLSETDLSPVKSKILNNLKKQPYVSGEFLSSQLNVSRTAIWKNIQGLANLGYQIDPIKGKGYRLVVIPDNLYPWEL
DFSLKKDYQITYFDNIDSTNNYARSLQPPQVVIAEQQTKGRGRMGRAWFSAPGGIYFSLVITPRCSVDEIPTIPLLISTA
ITKVLKRELGIQAEIKWPNDILVQGQKVTGILVELAGETDAPQKAVIGVGINANQNTNLFPEELKKSVTSLRELNCSPVN
RKLMLNSILEEITQLFFELPNCIPKVLNTWKEYSCTLGKKITVKQVSKTITGIAKDIDRSGALIVETPQGEQRILTGDLS
INI
>Mature_322_residues
SIIKLSETDLSPVKSKILNNLKKQPYVSGEFLSSQLNVSRTAIWKNIQGLANLGYQIDPIKGKGYRLVVIPDNLYPWELD
FSLKKDYQITYFDNIDSTNNYARSLQPPQVVIAEQQTKGRGRMGRAWFSAPGGIYFSLVITPRCSVDEIPTIPLLISTAI
TKVLKRELGIQAEIKWPNDILVQGQKVTGILVELAGETDAPQKAVIGVGINANQNTNLFPEELKKSVTSLRELNCSPVNR
KLMLNSILEEITQLFFELPNCIPKVLNTWKEYSCTLGKKITVKQVSKTITGIAKDIDRSGALIVETPQGEQRILTGDLSI
NI

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Escherichia coli, GI1790408, Length=297, Percent_Identity=30.3030303030303, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 35974; Mature: 35843

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIKLSETDLSPVKSKILNNLKKQPYVSGEFLSSQLNVSRTAIWKNIQGLANLGYQIDP
CCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEC
IKGKGYRLVVIPDNLYPWELDFSLKKDYQITYFDNIDSTNNYARSLQPPQVVIAEQQTKG
CCCCCEEEEEECCCCCCEEEEEEECCCEEEEEECCCCCCCHHHHCCCCCEEEEECCCCCC
RGRMGRAWFSAPGGIYFSLVITPRCSVDEIPTIPLLISTAITKVLKRELGIQAEIKWPND
CCCCCCCEECCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCC
ILVQGQKVTGILVELAGETDAPQKAVIGVGINANQNTNLFPEELKKSVTSLRELNCSPVN
EEEECCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCH
RKLMLNSILEEITQLFFELPNCIPKVLNTWKEYSCTLGKKITVKQVSKTITGIAKDIDRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCC
GALIVETPQGEQRILTGDLSINI
CCEEEECCCCCCEEEEEEEEEEC
>Mature Secondary Structure 
SIIKLSETDLSPVKSKILNNLKKQPYVSGEFLSSQLNVSRTAIWKNIQGLANLGYQIDP
CEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCEEEEC
IKGKGYRLVVIPDNLYPWELDFSLKKDYQITYFDNIDSTNNYARSLQPPQVVIAEQQTKG
CCCCCEEEEEECCCCCCEEEEEEECCCEEEEEECCCCCCCHHHHCCCCCEEEEECCCCCC
RGRMGRAWFSAPGGIYFSLVITPRCSVDEIPTIPLLISTAITKVLKRELGIQAEIKWPND
CCCCCCCEECCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCCC
ILVQGQKVTGILVELAGETDAPQKAVIGVGINANQNTNLFPEELKKSVTSLRELNCSPVN
EEEECCCEEEEEEEECCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCH
RKLMLNSILEEITQLFFELPNCIPKVLNTWKEYSCTLGKKITVKQVSKTITGIAKDIDRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHCCC
GALIVETPQGEQRILTGDLSINI
CCEEEECCCCCCEEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]