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The map label for this gene is prs [H]

Identifier: 188584703

GI number: 188584703

Start: 75631

End: 76599

Strand: Direct

Name: prs [H]

Synonym: Nther_0061

Alternate gene names: 188584703

Gene position: 75631-76599 (Clockwise)

Preceding gene: 188584702

Following gene: 188584704

Centisome position: 2.39

GC content: 38.29

Gene sequence:

>969_bases
ATGGCTAATGATGAAAAGCGACTTAATACTAAAAACTTAAAGATTTTTTCAGGAAATGCTAATCTAAATCTATCCCAAGA
AATAACAGAATATTTGGGAGTAGACATGGGAAAAGGCAAAGCTACAAGATTTAGTGATGGAGAAATTGATGTTGTAATTG
AAGAAAGTATTAGAGGTTCTGATGTATTTGTAGTACAACCTATTTGTTCTCCGGCAAATGAAAATATAATGGAACTGTTA
ATCATGTTGGATGCATTTAAAAGAGCATCAGCTCAAGAAATCAATGTAGTTATACCCTATTACGGTTATGCCAGAAAAGA
TAGAAAAGCTCGTGCTAGGGATCCAATAACAGCAAAATTAATGGCAGATTTACTGGAAACAGCTGGTGCCAATCGGGTCA
TTACCATGGACTTACATGCCCAACAAATTCAAGGCTTTTTCAATATTCCGCTAGATCATCTTCAAGCTGGGCCAATCCTA
GCCGACTATTTCCAAGAAAAAGGGTTAGATGAGCCTGTGATTGTTTCTCCTGATATGGGTGGTGTACCTAGAGCGAGAGC
CATGGCCGAGAACTTAAATTGTCCGATAGCCATAATTGATAAACGTCGTCCCGATGCCAATGTGGCCGAAATAATGAATG
TTGTCGGAGATGTTGAGGGGAAAACGGCTATCATGATTGACGACTTGATTGATACTGCAGGAACTATGTCACTAGCGGCG
GAAGCCCTATTAAAGCATGGGGCTAAAGATGTATACGCATGTTGTACACATCCTGTCTTAAGTGGCCCTGCAGTAGATAG
ATTGAGTAAATCACCCATTAAAGAAATAGTGGTTACTAATACAATTCCACTTCCAAAAGAAAAAGAACTGGATAAAATTA
AAGTTCTATCAGTGGCTCCTTTAATTGGGGAAGCAATTTTAAGGGTTCATAACAGAATGTCTGTAAGCACTTTATTTGAA
GATGACTAA

Upstream 100 bases:

>100_bases
GTAACAGAAAACATCCCTGAAGATAGCTTGGGGATTGCTCGATGCAGACAAACAAACAAAGAAGATTGGGTTACTAAAAA
AATGGGCAGGGGGTATAGTG

Downstream 100 bases:

>100_bases
TGTTTGACTAATTTCGCAAAAATAGATATACTAATGTTTGGTATATTTTTAACATAGATATTAAGCGCGAAAAGAAAGGG
GTATTATAGATGGATAGAAA

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MANDEKRLNTKNLKIFSGNANLNLSQEITEYLGVDMGKGKATRFSDGEIDVVIEESIRGSDVFVVQPICSPANENIMELL
IMLDAFKRASAQEINVVIPYYGYARKDRKARARDPITAKLMADLLETAGANRVITMDLHAQQIQGFFNIPLDHLQAGPIL
ADYFQEKGLDEPVIVSPDMGGVPRARAMAENLNCPIAIIDKRRPDANVAEIMNVVGDVEGKTAIMIDDLIDTAGTMSLAA
EALLKHGAKDVYACCTHPVLSGPAVDRLSKSPIKEIVVTNTIPLPKEKELDKIKVLSVAPLIGEAILRVHNRMSVSTLFE
DD

Sequences:

>Translated_322_residues
MANDEKRLNTKNLKIFSGNANLNLSQEITEYLGVDMGKGKATRFSDGEIDVVIEESIRGSDVFVVQPICSPANENIMELL
IMLDAFKRASAQEINVVIPYYGYARKDRKARARDPITAKLMADLLETAGANRVITMDLHAQQIQGFFNIPLDHLQAGPIL
ADYFQEKGLDEPVIVSPDMGGVPRARAMAENLNCPIAIIDKRRPDANVAEIMNVVGDVEGKTAIMIDDLIDTAGTMSLAA
EALLKHGAKDVYACCTHPVLSGPAVDRLSKSPIKEIVVTNTIPLPKEKELDKIKVLSVAPLIGEAILRVHNRMSVSTLFE
DD
>Mature_321_residues
ANDEKRLNTKNLKIFSGNANLNLSQEITEYLGVDMGKGKATRFSDGEIDVVIEESIRGSDVFVVQPICSPANENIMELLI
MLDAFKRASAQEINVVIPYYGYARKDRKARARDPITAKLMADLLETAGANRVITMDLHAQQIQGFFNIPLDHLQAGPILA
DYFQEKGLDEPVIVSPDMGGVPRARAMAENLNCPIAIIDKRRPDANVAEIMNVVGDVEGKTAIMIDDLIDTAGTMSLAAE
ALLKHGAKDVYACCTHPVLSGPAVDRLSKSPIKEIVVTNTIPLPKEKELDKIKVLSVAPLIGEAILRVHNRMSVSTLFED
D

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=311, Percent_Identity=47.9099678456592, Blast_Score=290, Evalue=1e-78,
Organism=Homo sapiens, GI28557709, Length=311, Percent_Identity=47.2668810289389, Blast_Score=289, Evalue=2e-78,
Organism=Homo sapiens, GI4506129, Length=311, Percent_Identity=46.3022508038585, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI84875539, Length=314, Percent_Identity=45.859872611465, Blast_Score=281, Evalue=5e-76,
Organism=Homo sapiens, GI4506133, Length=356, Percent_Identity=40.4494382022472, Blast_Score=206, Evalue=2e-53,
Organism=Homo sapiens, GI194018537, Length=344, Percent_Identity=38.0813953488372, Blast_Score=195, Evalue=5e-50,
Organism=Homo sapiens, GI310128524, Length=144, Percent_Identity=29.8611111111111, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI310115209, Length=144, Percent_Identity=29.8611111111111, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI310118259, Length=144, Percent_Identity=29.8611111111111, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI310119946, Length=144, Percent_Identity=29.8611111111111, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1787458, Length=312, Percent_Identity=50.6410256410256, Blast_Score=332, Evalue=3e-92,
Organism=Caenorhabditis elegans, GI17554702, Length=321, Percent_Identity=46.1059190031153, Blast_Score=290, Evalue=8e-79,
Organism=Caenorhabditis elegans, GI71989924, Length=321, Percent_Identity=46.1059190031153, Blast_Score=288, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI25149168, Length=311, Percent_Identity=46.9453376205788, Blast_Score=288, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI17554704, Length=308, Percent_Identity=46.7532467532467, Blast_Score=283, Evalue=6e-77,
Organism=Caenorhabditis elegans, GI17570245, Length=342, Percent_Identity=33.0409356725146, Blast_Score=186, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6320946, Length=315, Percent_Identity=44.4444444444444, Blast_Score=263, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6319403, Length=327, Percent_Identity=42.8134556574924, Blast_Score=259, Evalue=3e-70,
Organism=Saccharomyces cerevisiae, GI6321776, Length=315, Percent_Identity=42.8571428571429, Blast_Score=257, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6322667, Length=197, Percent_Identity=39.5939086294416, Blast_Score=149, Evalue=7e-37,
Organism=Saccharomyces cerevisiae, GI6324511, Length=110, Percent_Identity=42.7272727272727, Blast_Score=95, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21355239, Length=313, Percent_Identity=46.0063897763578, Blast_Score=278, Evalue=2e-75,
Organism=Drosophila melanogaster, GI45551540, Length=336, Percent_Identity=43.1547619047619, Blast_Score=267, Evalue=6e-72,
Organism=Drosophila melanogaster, GI281362873, Length=365, Percent_Identity=36.986301369863, Blast_Score=214, Evalue=8e-56,
Organism=Drosophila melanogaster, GI24651454, Length=365, Percent_Identity=36.986301369863, Blast_Score=214, Evalue=8e-56,
Organism=Drosophila melanogaster, GI24651458, Length=359, Percent_Identity=37.3259052924791, Blast_Score=214, Evalue=9e-56,
Organism=Drosophila melanogaster, GI24651456, Length=359, Percent_Identity=37.3259052924791, Blast_Score=214, Evalue=9e-56,
Organism=Drosophila melanogaster, GI45552010, Length=211, Percent_Identity=38.3886255924171, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24651462, Length=205, Percent_Identity=39.0243902439024, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24651464, Length=205, Percent_Identity=39.0243902439024, Blast_Score=140, Evalue=1e-33,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 35206; Mature: 35075

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDEKRLNTKNLKIFSGNANLNLSQEITEYLGVDMGKGKATRFSDGEIDVVIEESIRGS
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCC
DVFVVQPICSPANENIMELLIMLDAFKRASAQEINVVIPYYGYARKDRKARARDPITAKL
CEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHCCCCHHHHH
MADLLETAGANRVITMDLHAQQIQGFFNIPLDHLQAGPILADYFQEKGLDEPVIVSPDMG
HHHHHHHCCCCEEEEEECCHHHHCHHHCCCHHHHCCCCHHHHHHHHCCCCCCEEECCCCC
GVPRARAMAENLNCPIAIIDKRRPDANVAEIMNVVGDVEGKTAIMIDDLIDTAGTMSLAA
CCCHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHCCHHHHHHH
EALLKHGAKDVYACCTHPVLSGPAVDRLSKSPIKEIVVTNTIPLPKEKELDKIKVLSVAP
HHHHHCCCHHHHHHHCCCCCCCCHHHHHHHCCHHHHHEECCCCCCCCCCCCCEEEEHHHH
LIGEAILRVHNRMSVSTLFEDD
HHHHHHHHHHCCCCCHHHCCCC
>Mature Secondary Structure 
ANDEKRLNTKNLKIFSGNANLNLSQEITEYLGVDMGKGKATRFSDGEIDVVIEESIRGS
CCCCCCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCC
DVFVVQPICSPANENIMELLIMLDAFKRASAQEINVVIPYYGYARKDRKARARDPITAKL
CEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCHHHCCCCHHHHH
MADLLETAGANRVITMDLHAQQIQGFFNIPLDHLQAGPILADYFQEKGLDEPVIVSPDMG
HHHHHHHCCCCEEEEEECCHHHHCHHHCCCHHHHCCCCHHHHHHHHCCCCCCEEECCCCC
GVPRARAMAENLNCPIAIIDKRRPDANVAEIMNVVGDVEGKTAIMIDDLIDTAGTMSLAA
CCCHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHCCCCCEEEEEHHHHHCCHHHHHHH
EALLKHGAKDVYACCTHPVLSGPAVDRLSKSPIKEIVVTNTIPLPKEKELDKIKVLSVAP
HHHHHCCCHHHHHHHCCCCCCCCHHHHHHHCCHHHHHEECCCCCCCCCCCCCEEEEHHHH
LIGEAILRVHNRMSVSTLFEDD
HHHHHHHHHHCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]