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The map label for this gene is ispE [H]

Identifier: 188584698

GI number: 188584698

Start: 71216

End: 72142

Strand: Direct

Name: ispE [H]

Synonym: Nther_0056

Alternate gene names: 188584698

Gene position: 71216-72142 (Clockwise)

Preceding gene: 188584697

Following gene: 188584699

Centisome position: 2.25

GC content: 36.79

Gene sequence:

>927_bases
TTGTCACCAGATATTTTGAGAGTGGAATCTCCTGCTAAGATTAATCTCTACTTAGATATTTTAGGGAAGAGGCAGGATGG
TTTCCATGAAGTTGAAATGGTAATGCAATCGATTTCTTTATGTGATCGGTTAACTTTTATAAGACAATCCCAGGGTAACA
ATAACAATATTGAATTGCTAATGAAATCATCGGACTGTATTGACCAATTACCTGTCAGGGGAGATAACTTAATAATTAAG
GCTGCCAGGTTATTGATGAATGATTTTAGATTACCGCCAATCAAGGTGATTTTAGAGAAGAACATTCCAACTGAAGCGGG
GTTAGCCGGTGGCAGTAGTAATGCTGCTGCAACTTTATGGGCAATAAATCACATGTTTCAACTAGGACTTACCGAGCAAG
AACTATCTGACATAGGTGCTCGCCTTGGTTCAGATATTCCCTTTTGTTTGTTTGGCGGAACCAAGCTTGCCAAGGGACGA
GGAGAAATTTTACATCCACTACCTTCTTTGCCCAACTGTTATTTTGTACTAGTTAAACCCAATTTTGGAGTTAGTACGGG
AAAAGTGTATCAAGAGCTAGGGTTTAAAACGGATACCGAATATGGAGAAAATCATCAAACAAAATCAAGGGTCAATGGAA
TTATTTCAGGCTTGGAAAAAGGAACTTTAACTGGAATTGTAGAAAATATGTATAATAAGATGGAAGAGATTGTGTTTAAA
TGGCATAGGGATATGCAAATAATATCACAGCAAATTGAGCAATTAGGGGCTTTAAAAGTATTAATGAGTGGAAGTGGTTC
AACTATTTTTGGCGTTTTTGATAACTATGACACTGCTAAATATGCAAAAAAACAATTAGAAAGAGAATTTAAGTATGTGT
TTCTCTCGATCCCCCGTGATATGGGAGTGGGAAAGGAGAATAATTAA

Upstream 100 bases:

>100_bases
GATATCATTATCAAGGAGTTGAAGGAGTTGGAGGAGTTGCAAAGAGTGAAGTATATCTAAGATATATGGACTACTAAAGT
TCTGAGGAGGGGAGCTTAAC

Downstream 100 bases:

>100_bases
TGGTTCAATGTGTTGTGTTAGCAGGAAATTCTGAAACTGATAGAAAAGTAGCAGATTTAACATCTCAAAATATTAATAAC
AAGGCACTGGTACCTATTAA

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MSPDILRVESPAKINLYLDILGKRQDGFHEVEMVMQSISLCDRLTFIRQSQGNNNNIELLMKSSDCIDQLPVRGDNLIIK
AARLLMNDFRLPPIKVILEKNIPTEAGLAGGSSNAAATLWAINHMFQLGLTEQELSDIGARLGSDIPFCLFGGTKLAKGR
GEILHPLPSLPNCYFVLVKPNFGVSTGKVYQELGFKTDTEYGENHQTKSRVNGIISGLEKGTLTGIVENMYNKMEEIVFK
WHRDMQIISQQIEQLGALKVLMSGSGSTIFGVFDNYDTAKYAKKQLEREFKYVFLSIPRDMGVGKENN

Sequences:

>Translated_308_residues
MSPDILRVESPAKINLYLDILGKRQDGFHEVEMVMQSISLCDRLTFIRQSQGNNNNIELLMKSSDCIDQLPVRGDNLIIK
AARLLMNDFRLPPIKVILEKNIPTEAGLAGGSSNAAATLWAINHMFQLGLTEQELSDIGARLGSDIPFCLFGGTKLAKGR
GEILHPLPSLPNCYFVLVKPNFGVSTGKVYQELGFKTDTEYGENHQTKSRVNGIISGLEKGTLTGIVENMYNKMEEIVFK
WHRDMQIISQQIEQLGALKVLMSGSGSTIFGVFDNYDTAKYAKKQLEREFKYVFLSIPRDMGVGKENN
>Mature_307_residues
SPDILRVESPAKINLYLDILGKRQDGFHEVEMVMQSISLCDRLTFIRQSQGNNNNIELLMKSSDCIDQLPVRGDNLIIKA
ARLLMNDFRLPPIKVILEKNIPTEAGLAGGSSNAAATLWAINHMFQLGLTEQELSDIGARLGSDIPFCLFGGTKLAKGRG
EILHPLPSLPNCYFVLVKPNFGVSTGKVYQELGFKTDTEYGENHQTKSRVNGIISGLEKGTLTGIVENMYNKMEEIVFKW
HRDMQIISQQIEQLGALKVLMSGSGSTIFGVFDNYDTAKYAKKQLEREFKYVFLSIPRDMGVGKENN

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=287, Percent_Identity=31.3588850174216, Blast_Score=114, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 34367; Mature: 34236

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPDILRVESPAKINLYLDILGKRQDGFHEVEMVMQSISLCDRLTFIRQSQGNNNNIELL
CCCCCEEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
MKSSDCIDQLPVRGDNLIIKAARLLMNDFRLPPIKVILEKNIPTEAGLAGGSSNAAATLW
EECCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHCCCCCCCCCHHHHH
AINHMFQLGLTEQELSDIGARLGSDIPFCLFGGTKLAKGRGEILHPLPSLPNCYFVLVKP
HHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCEECCCCCCCCCCCCCCCCEEEEEECC
NFGVSTGKVYQELGFKTDTEYGENHQTKSRVNGIISGLEKGTLTGIVENMYNKMEEIVFK
CCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WHRDMQIISQQIEQLGALKVLMSGSGSTIFGVFDNYDTAKYAKKQLEREFKYVFLSIPRD
HHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHEEEEECCHH
MGVGKENN
CCCCCCCC
>Mature Secondary Structure 
SPDILRVESPAKINLYLDILGKRQDGFHEVEMVMQSISLCDRLTFIRQSQGNNNNIELL
CCCCEEECCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
MKSSDCIDQLPVRGDNLIIKAARLLMNDFRLPPIKVILEKNIPTEAGLAGGSSNAAATLW
EECCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHCCCCCCCCCHHHHH
AINHMFQLGLTEQELSDIGARLGSDIPFCLFGGTKLAKGRGEILHPLPSLPNCYFVLVKP
HHHHHHHHCCCHHHHHHHHHHHCCCCCEEEECCCEECCCCCCCCCCCCCCCCEEEEEECC
NFGVSTGKVYQELGFKTDTEYGENHQTKSRVNGIISGLEKGTLTGIVENMYNKMEEIVFK
CCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
WHRDMQIISQQIEQLGALKVLMSGSGSTIFGVFDNYDTAKYAKKQLEREFKYVFLSIPRD
HHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHEEEEECCHH
MGVGKENN
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA