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The map label for this gene is cphA [H]

Identifier: 188584697

GI number: 188584697

Start: 68430

End: 71114

Strand: Direct

Name: cphA [H]

Synonym: Nther_0055

Alternate gene names: 188584697

Gene position: 68430-71114 (Clockwise)

Preceding gene: 188584696

Following gene: 188584698

Centisome position: 2.16

GC content: 38.62

Gene sequence:

>2685_bases
TTGGAAATTATTAATATTAAAACAATTTCAGGCAAAAACATATACAGTCATGAACCGGTTCTAGAAGCAGAATTAGACTT
AGGTGATTTTGCAGGGGTTTTCTCCAATAATGTATCCTGTTTTAATAGTAGGTTGCACCAAATTTTACCGTCTTTGCAGG
AGCATGTCTGTTCTAGAGGATATCCTGGTGGATTTTTGGAACGGTTGGAAGAAGGTACTTTGTTTGGTCATATAATTGAG
CATGTAACTTTAGAATTAATGAATTTAGCCGGCATTGGAACCAATTATGGGACAACGAAAGCCACTCAAGAAGACGGGAT
CTATCAAGTGATCGTGGAACAGATCAATGAAGAAGGTGCCAAATATGCTCTAGAACAGTCCGTTGAACTTGTGAAAAACG
TCATAAATGACAATCCCGTTCAAGTAGAGGAAATAGTTAGCGAAATTCAAGAAATAGTTAACGAAACGAAGATTGGTCCT
AGTACTGAAGCAATTTTATCAGCTGCCGAGGAACAGGAGATTCCTTATCTCAAACTGGATGAAGCTAGTATGTACCAACT
GGGCACAGGCAAGTATCAAAAGAGAATCCAGGCTACTGTAACTGATGAAACTTCTGTAATAGGAGTGGATATAGCCTGTG
ATAAAACCAAAACAAATAACTCCTTGCATGAATTAGGTTTACCAGTTCCTTATGGCAAAATAGCAAGTACTGAGGAAGAA
GCAGTAGCTATTGTACAGGAGATTGGATACCCTATTGTTATAAAACCCAGAGATGGAAATCAGGGAAAAGGAGTTACATT
AAATATCAACTCAGAACAGGAAACTAGAAGTGCCTTTAAAGTAGCTATGAGCTACGGGGATGAGGCGATTGTGGAACAGC
ATATTGAAGGAAAGCATTACCGTCTTGTAGTAGTAGGGGATCAATTGGTGGCCGGAGCTGAAAGAATCCCTGCCCATGTA
GTGGGAGATGGTCAACATACCATTAGAGAGCTAGTAGATATTGAAAATCAAAATCCACTTAGAGGTTTTGGTCATGAGAA
ACCTCTATCAAAAATTCAATTGGATCCAGTAGTTAATATGGTATTGGCTCGTAAGAATATGACTATGAATTACATCCCCC
AACAAGATGAACTAGTATATTTACGGGAAAGTGCCAATCTTAGCACGGGTGGAATTAGTTTGGATGTTACTGATGAGATT
TCTGATAAAACTATACGGATAGCCATCAGGGTAGCTAAAATAGTTGGATTAGATATAGCCGGTGTTGATCTGGTAGCTAA
GGATATTGGGAAACCCGTGGATGAAGATGATAATGGGGCTATTATTGAAGTCAATGCTGCCCCAGGAATAAGAATGCATG
AACATCCTGTGAAAGGCCATCCTCGAAGAGTAGGAAAAAGTATTGTAGATCACTTGTTTAACAAACGAAATGATACTTTA
CAGGCAAGAGCGCCAGTAATTTCAGTTACAGGTACAAACGGCAAAACAACTACTGTTAGAGCAATTGATCATATATTGAG
ATCAAATGGAAAAGATGTGGGCATGACAAGTAGTGATGGTACTTTTATCAAAGGAGAATTGATCGATTCTGGTGACAATA
CAGGCCCGAATAGTGCTAAGGCTTTATTAAAAGATCCGACAATTGATTCATTTGTGTTTGAAACAGCCCGAGGTGGACTG
GTAAAAAATGGGCTAGGTTATTCAAATGCCAAAGTGGCAGTAGTGACTAATATCGCAAATGATCACCTGGGCCAAGATGG
CATTGAATCAATTGACGATCTTATTTTTGTTAAAAGCCTAGTGGTTGAAGCTGTTGAGAAACAAGGTGTCGTTGTCTTGA
ACGCTAGCGATCACTATGTATCTGATTTCATTAGAAAGGCTAAAGGGAGAATAATATTTTACGCCATGGAATCCGATAAT
CATTATGTTAAATCACATTTAGCGGCGGGAGGATCAGCACTATATTTAGATCAGGATAATATTGTATACGCTACTGGGAA
ATTTCACAGAAAATTAGGGGAGATAACTCAATTGCCTCTAACCATGGCTGGTATGGCTCGTCATAATATAGAAAATTGCA
TGGCAGGGATAGCTGCTGCTATTGGTTTCGGCATAGCTCCTAGCAAAGCATTTGAAGTTTTAAAGCAATTTGCTCCAAGC
TATCAAGATACCCCGGGAAGATGTAATTTGTTTCGTTGTCAAGGAAAAACAGTCTTACTCGATTATGGTCACAACCCCGA
AGGGATGAATCGAGTGTTAGCAATGGTAGAAAAAATGCCAGTAGAAAAAGTGATAAGTGTGATTGGAGTACCTGGTGACA
GGAATGACAAACTGATTAAAGAAACGGGAAAAGTGGTGGGAAAGTATCATGAATTAATCGAGAAAGTTCTAGTCAAAGAA
GATTCGGATTTAAGGGGGAGAAAGTCTGGTGAAACAGCGAATCTGTTACAAACAACTATGACCGAATTTTCAGAAGAACT
GGCTGTAGAACAAAACTTACAAGAAATTGAAGCAATAAAAACAGCTCTTTCAGAAGCGGGTGAAAATGATTTGGTTCTAA
TATTTTACGAACAAAAAGACAGTTTCTTTAATGCATCTGAAGAGCTAGGCTTATCTCCGGAACCTTTAGACTTTAATAAA
TTGGCATCCCAATCTAATAATCCAGTTTCCCAAAAACTCAATTAA

Upstream 100 bases:

>100_bases
CTCCCCCTTAGCCCTTACTAATGTAACCGTGGATATACTGTCTCCAGGCTCACGCTTTGATTTAAAAAAAAGAAATTTGT
CCTTGCAAGGAGGATAAATA

Downstream 100 bases:

>100_bases
TGATATCATTATCAAGGAGTTGAAGGAGTTGGAGGAGTTGCAAAGAGTGAAGTATATCTAAGATATATGGACTACTAAAG
TTCTGAGGAGGGGAGCTTAA

Product: cyanophycin synthetase

Products: NA

Alternate protein names: Cyanophycin synthase [H]

Number of amino acids: Translated: 894; Mature: 894

Protein sequence:

>894_residues
MEIINIKTISGKNIYSHEPVLEAELDLGDFAGVFSNNVSCFNSRLHQILPSLQEHVCSRGYPGGFLERLEEGTLFGHIIE
HVTLELMNLAGIGTNYGTTKATQEDGIYQVIVEQINEEGAKYALEQSVELVKNVINDNPVQVEEIVSEIQEIVNETKIGP
STEAILSAAEEQEIPYLKLDEASMYQLGTGKYQKRIQATVTDETSVIGVDIACDKTKTNNSLHELGLPVPYGKIASTEEE
AVAIVQEIGYPIVIKPRDGNQGKGVTLNINSEQETRSAFKVAMSYGDEAIVEQHIEGKHYRLVVVGDQLVAGAERIPAHV
VGDGQHTIRELVDIENQNPLRGFGHEKPLSKIQLDPVVNMVLARKNMTMNYIPQQDELVYLRESANLSTGGISLDVTDEI
SDKTIRIAIRVAKIVGLDIAGVDLVAKDIGKPVDEDDNGAIIEVNAAPGIRMHEHPVKGHPRRVGKSIVDHLFNKRNDTL
QARAPVISVTGTNGKTTTVRAIDHILRSNGKDVGMTSSDGTFIKGELIDSGDNTGPNSAKALLKDPTIDSFVFETARGGL
VKNGLGYSNAKVAVVTNIANDHLGQDGIESIDDLIFVKSLVVEAVEKQGVVVLNASDHYVSDFIRKAKGRIIFYAMESDN
HYVKSHLAAGGSALYLDQDNIVYATGKFHRKLGEITQLPLTMAGMARHNIENCMAGIAAAIGFGIAPSKAFEVLKQFAPS
YQDTPGRCNLFRCQGKTVLLDYGHNPEGMNRVLAMVEKMPVEKVISVIGVPGDRNDKLIKETGKVVGKYHELIEKVLVKE
DSDLRGRKSGETANLLQTTMTEFSEELAVEQNLQEIEAIKTALSEAGENDLVLIFYEQKDSFFNASEELGLSPEPLDFNK
LASQSNNPVSQKLN

Sequences:

>Translated_894_residues
MEIINIKTISGKNIYSHEPVLEAELDLGDFAGVFSNNVSCFNSRLHQILPSLQEHVCSRGYPGGFLERLEEGTLFGHIIE
HVTLELMNLAGIGTNYGTTKATQEDGIYQVIVEQINEEGAKYALEQSVELVKNVINDNPVQVEEIVSEIQEIVNETKIGP
STEAILSAAEEQEIPYLKLDEASMYQLGTGKYQKRIQATVTDETSVIGVDIACDKTKTNNSLHELGLPVPYGKIASTEEE
AVAIVQEIGYPIVIKPRDGNQGKGVTLNINSEQETRSAFKVAMSYGDEAIVEQHIEGKHYRLVVVGDQLVAGAERIPAHV
VGDGQHTIRELVDIENQNPLRGFGHEKPLSKIQLDPVVNMVLARKNMTMNYIPQQDELVYLRESANLSTGGISLDVTDEI
SDKTIRIAIRVAKIVGLDIAGVDLVAKDIGKPVDEDDNGAIIEVNAAPGIRMHEHPVKGHPRRVGKSIVDHLFNKRNDTL
QARAPVISVTGTNGKTTTVRAIDHILRSNGKDVGMTSSDGTFIKGELIDSGDNTGPNSAKALLKDPTIDSFVFETARGGL
VKNGLGYSNAKVAVVTNIANDHLGQDGIESIDDLIFVKSLVVEAVEKQGVVVLNASDHYVSDFIRKAKGRIIFYAMESDN
HYVKSHLAAGGSALYLDQDNIVYATGKFHRKLGEITQLPLTMAGMARHNIENCMAGIAAAIGFGIAPSKAFEVLKQFAPS
YQDTPGRCNLFRCQGKTVLLDYGHNPEGMNRVLAMVEKMPVEKVISVIGVPGDRNDKLIKETGKVVGKYHELIEKVLVKE
DSDLRGRKSGETANLLQTTMTEFSEELAVEQNLQEIEAIKTALSEAGENDLVLIFYEQKDSFFNASEELGLSPEPLDFNK
LASQSNNPVSQKLN
>Mature_894_residues
MEIINIKTISGKNIYSHEPVLEAELDLGDFAGVFSNNVSCFNSRLHQILPSLQEHVCSRGYPGGFLERLEEGTLFGHIIE
HVTLELMNLAGIGTNYGTTKATQEDGIYQVIVEQINEEGAKYALEQSVELVKNVINDNPVQVEEIVSEIQEIVNETKIGP
STEAILSAAEEQEIPYLKLDEASMYQLGTGKYQKRIQATVTDETSVIGVDIACDKTKTNNSLHELGLPVPYGKIASTEEE
AVAIVQEIGYPIVIKPRDGNQGKGVTLNINSEQETRSAFKVAMSYGDEAIVEQHIEGKHYRLVVVGDQLVAGAERIPAHV
VGDGQHTIRELVDIENQNPLRGFGHEKPLSKIQLDPVVNMVLARKNMTMNYIPQQDELVYLRESANLSTGGISLDVTDEI
SDKTIRIAIRVAKIVGLDIAGVDLVAKDIGKPVDEDDNGAIIEVNAAPGIRMHEHPVKGHPRRVGKSIVDHLFNKRNDTL
QARAPVISVTGTNGKTTTVRAIDHILRSNGKDVGMTSSDGTFIKGELIDSGDNTGPNSAKALLKDPTIDSFVFETARGGL
VKNGLGYSNAKVAVVTNIANDHLGQDGIESIDDLIFVKSLVVEAVEKQGVVVLNASDHYVSDFIRKAKGRIIFYAMESDN
HYVKSHLAAGGSALYLDQDNIVYATGKFHRKLGEITQLPLTMAGMARHNIENCMAGIAAAIGFGIAPSKAFEVLKQFAPS
YQDTPGRCNLFRCQGKTVLLDYGHNPEGMNRVLAMVEKMPVEKVISVIGVPGDRNDKLIKETGKVVGKYHELIEKVLVKE
DSDLRGRKSGETANLLQTTMTEFSEELAVEQNLQEIEAIKTALSEAGENDLVLIFYEQKDSFFNASEELGLSPEPLDFNK
LASQSNNPVSQKLN

Specific function: Catalyzes the ATP-dependent polymerization of arginine and aspartate to multi-L-arginyl-poly-L-aspartic acid (cyanophycin; a water-insoluble reserve polymer) [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

None

Paralogues:

None

Copy number: 4701 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011810
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; PF08443 RimK [H]

EC number: =6.3.2.29; =6.3.2.30 [H]

Molecular weight: Translated: 97650; Mature: 97650

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: PS50975 ATP_GRASP ; PS01011 FOLYLPOLYGLU_SYNT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIINIKTISGKNIYSHEPVLEAELDLGDFAGVFSNNVSCFNSRLHQILPSLQEHVCSRG
CEEEEEEEECCCCCCCCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
YPGGFLERLEEGTLFGHIIEHVTLELMNLAGIGTNYGTTKATQEDGIYQVIVEQINEEGA
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KYALEQSVELVKNVINDNPVQVEEIVSEIQEIVNETKIGPSTEAILSAAEEQEIPYLKLD
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEC
EASMYQLGTGKYQKRIQATVTDETSVIGVDIACDKTKTNNSLHELGLPVPYGKIASTEEE
CCCCEECCCCHHHHHEEEEECCCCEEEEEEEEECCCCCCCCHHHCCCCCCCCCCCCCHHH
AVAIVQEIGYPIVIKPRDGNQGKGVTLNINSEQETRSAFKVAMSYGDEAIVEQHIEGKHY
HHHHHHHCCCCEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHCCCCEE
RLVVVGDQLVAGAERIPAHVVGDGQHTIRELVDIENQNPLRGFGHEKPLSKIQLDPVVNM
EEEEECCHHHHCHHHCCCEEECCCHHHHHHHHCCCCCCCCCCCCCCCCHHHEEHHHHHHH
VLARKNMTMNYIPQQDELVYLRESANLSTGGISLDVTDEISDKTIRIAIRVAKIVGLDIA
HHHHCCCCEEECCCCCCEEEEECCCCCCCCCEEEEECCCCCCCEEEEEEEEEHHHCCCCC
GVDLVAKDIGKPVDEDDNGAIIEVNAAPGIRMHEHPVKGHPRRVGKSIVDHLFNKRNDTL
CHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCE
QARAPVISVTGTNGKTTTVRAIDHILRSNGKDVGMTSSDGTFIKGELIDSGDNTGPNSAK
EECCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHH
ALLKDPTIDSFVFETARGGLVKNGLGYSNAKVAVVTNIANDHLGQDGIESIDDLIFVKSL
HHHCCCCHHHHHHHHHCCCCEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
VVEAVEKQGVVVLNASDHYVSDFIRKAKGRIIFYAMESDNHYVKSHLAAGGSALYLDQDN
HHHHHHHCCEEEEECCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCCEEEEECCC
IVYATGKFHRKLGEITQLPLTMAGMARHNIENCMAGIAAAIGFGIAPSKAFEVLKQFAPS
EEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
YQDTPGRCNLFRCQGKTVLLDYGHNPEGMNRVLAMVEKMPVEKVISVIGVPGDRNDKLIK
CCCCCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHH
ETGKVVGKYHELIEKVLVKEDSDLRGRKSGETANLLQTTMTEFSEELAVEQNLQEIEAIK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TALSEAGENDLVLIFYEQKDSFFNASEELGLSPEPLDFNKLASQSNNPVSQKLN
HHHHHCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCHHHHHCCCCCCHHHCCC
>Mature Secondary Structure
MEIINIKTISGKNIYSHEPVLEAELDLGDFAGVFSNNVSCFNSRLHQILPSLQEHVCSRG
CEEEEEEEECCCCCCCCCCCEEEECCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCC
YPGGFLERLEEGTLFGHIIEHVTLELMNLAGIGTNYGTTKATQEDGIYQVIVEQINEEGA
CCCHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
KYALEQSVELVKNVINDNPVQVEEIVSEIQEIVNETKIGPSTEAILSAAEEQEIPYLKLD
HHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEEC
EASMYQLGTGKYQKRIQATVTDETSVIGVDIACDKTKTNNSLHELGLPVPYGKIASTEEE
CCCCEECCCCHHHHHEEEEECCCCEEEEEEEEECCCCCCCCHHHCCCCCCCCCCCCCHHH
AVAIVQEIGYPIVIKPRDGNQGKGVTLNINSEQETRSAFKVAMSYGDEAIVEQHIEGKHY
HHHHHHHCCCCEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHCCCCEE
RLVVVGDQLVAGAERIPAHVVGDGQHTIRELVDIENQNPLRGFGHEKPLSKIQLDPVVNM
EEEEECCHHHHCHHHCCCEEECCCHHHHHHHHCCCCCCCCCCCCCCCCHHHEEHHHHHHH
VLARKNMTMNYIPQQDELVYLRESANLSTGGISLDVTDEISDKTIRIAIRVAKIVGLDIA
HHHHCCCCEEECCCCCCEEEEECCCCCCCCCEEEEECCCCCCCEEEEEEEEEHHHCCCCC
GVDLVAKDIGKPVDEDDNGAIIEVNAAPGIRMHEHPVKGHPRRVGKSIVDHLFNKRNDTL
CHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCE
QARAPVISVTGTNGKTTTVRAIDHILRSNGKDVGMTSSDGTFIKGELIDSGDNTGPNSAK
EECCCEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCHHH
ALLKDPTIDSFVFETARGGLVKNGLGYSNAKVAVVTNIANDHLGQDGIESIDDLIFVKSL
HHHCCCCHHHHHHHHHCCCCEECCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHH
VVEAVEKQGVVVLNASDHYVSDFIRKAKGRIIFYAMESDNHYVKSHLAAGGSALYLDQDN
HHHHHHHCCEEEEECCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCCCEEEEECCC
IVYATGKFHRKLGEITQLPLTMAGMARHNIENCMAGIAAAIGFGIAPSKAFEVLKQFAPS
EEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
YQDTPGRCNLFRCQGKTVLLDYGHNPEGMNRVLAMVEKMPVEKVISVIGVPGDRNDKLIK
CCCCCCCCEEEEECCCEEEEECCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHH
ETGKVVGKYHELIEKVLVKEDSDLRGRKSGETANLLQTTMTEFSEELAVEQNLQEIEAIK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TALSEAGENDLVLIFYEQKDSFFNASEELGLSPEPLDFNKLASQSNNPVSQKLN
HHHHHCCCCCEEEEEECCCCCCCCCHHHCCCCCCCCCHHHHHCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11131019 [H]