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The map label for this gene is metG [H]

Identifier: 188584685

GI number: 188584685

Start: 57085

End: 59052

Strand: Direct

Name: metG [H]

Synonym: Nther_0043

Alternate gene names: 188584685

Gene position: 57085-59052 (Clockwise)

Preceding gene: 188584683

Following gene: 188584686

Centisome position: 1.8

GC content: 37.14

Gene sequence:

>1968_bases
ATGGCCAGGGATAACACATTCTATATTACTACCCCGATTTACTATCCCAGTGATAAGTTACATATTGGGCACTCGTACAC
TACAGTGGCTGCTGATTGTATGGCTCGATATAAAAAGTTAACGGGATATGATGTTATGTTTTTGACTGGTACCGATGAAC
ACGGCCAAAAAATTGAACGAAAAGCAAAAGAAAATGGGAAAAACCCCCAGCAATTTGTAGATGAAATTGTAGCTAGCATT
AAAGAGCTGTGGACATTACTTGATATTGAATATGATGATTTTATTAGAACCACCGAGGAAAGACACAAATCTGTAGTCCA
AAAAATATTTGAACGTTTTTACGAGCAGGGAGATATTTACAAAGACCAGTACGAAGGCTGGTACTGCACTCCTTGCGAAT
CTTTTTGGCTGGAGAGACAGCTTGATGAAGCTAAAACATGCCCGGATTGTGCTCGAGAAGTAGAATGGGTAAAAGAAGAG
AGTTACTTCTTTAAAATGAGTAAATATGCCGATCGCTTGTTAGAGCATATTGAAAATAATCCAGAGTTTATTCAACCCGA
GTCACGGAAAAATGAGATGATCAATAACTTTTTAAACCCTGGTTTGGAGGACTTATGCGTTTCTCGAACAACCTTTAAAT
GGGGTATTGAGGTCCCCATGGATAAAGACCATGTAGTATACGTATGGTTAGATGCTCTGACAAACTACTTAAGTGCATTA
GGATACTTATCAGAAGATGATACAAAATTTCAAAAGTATTGGCCATGCGATGTACATTTGATGGCCAAGGAGATAGTTAG
ATTCCATTCGATTTATTGGCCTATTTTCCTTATGGCTTTAGACCTTCCTTTACCTAAACAGGTATTTGGCCATGGCTGGC
TTCTGTTGGAGGAAGGAAAAATGAGTAAATCAAAGGGAAATGTAGTAGATCCTGAAGTTTTAGTTAATAAGTACGGTTCA
GATGCCATCAGATATTATTTGTTGCGAGAAATATCTTTTGGATCTGATGGAGTGTTCACCTTAGAAGCATTAATTCGTAG
AATAAATTACGACTTAGCTAATGATTTGGGTAATCTGTTAAACAGAACCGTGGCCATGATTGACAAATACTTTGATGGTC
AGATTCCAGAACCAGTTGTTTCAGAACAGGTGGATAATGACTTGATTAATCATGCACAAGAAACAGTGACAGAGACAGAA
AAACACATGGAAGGCATGGATTTCTCGGAAGCATTAAAAGCTCTATGGGAGCTAATAGGGAGAACTAACAAGTATATCGA
TGAAACACAACCCTGGATTCTTGGAAAAGATCCTAACAAAAAAGATAGATTAGCTACAGTACTTTATAATTTATCTGAAA
GTTTACGATATATTTCTGTATTGTTATCACCTTTTATGCCCAGGACGCCAAAGCAAATAAGGAGTCAACTGGGAATAGAT
AATAACAAAGAAATCCAAACTTATAAGTCTTTGTATGAGTGGGGCCAAATTCCTTCTGGAGTTAGGGTTTCTAAGGATAA
ACCATTGTTTCCAAGATTGGAAATAGAAGAGGAATTAGAGTCTTTCAATGACAACAGTGAAGAACCACAAAAAGAAGAGA
AAGAACAGGATTCTCTGAAATATCCAGAACTGAAAGAACAAGTAGATATTAAGGATTTTCAAAAACTTGATTTAAGAGTA
GCTGAAATTGTCAATGTGGAGCCTATCAAAGGCGCAGACAAATTATGGAAGGTCAATGTAGATTTAGGTTTTGAAAATAG
ACAGGTAGTTGCGGGTATCAAGGAAAGTTTTGGTGCTGAAGATTTGATTGGGAAAAAAGTTATCTTGGTGGCTAATTTAA
AGCCGGCCAAAATAAAAGGAGTTAAGTCCCAGGGAATGATCCTAGCTGCCAATGATGAAGGTACAAATCAGTTGGAATTA
CCCAATTTTATCTCAGACAAAATCACTAAAGGAAGTCAGGTGAGGTAA

Upstream 100 bases:

>100_bases
TAAGGGATTGTGGCTTTTTTATCCAATAAAAATTAATGAATAGGCCTTCATCCCGGGGGTTGAAGGCTTTTTCTATTAAA
AATTTGAGGGAGGTTTTGCT

Downstream 100 bases:

>100_bases
ATTGAATATTATTGATACACATGCTCATCTAGATGATAAACGCTTTTCTTCTGATTTGGAAGATGTGATTGAAAGGGCTA
AACAAAAGAAAGTCTCTCAG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 655; Mature: 654

Protein sequence:

>655_residues
MARDNTFYITTPIYYPSDKLHIGHSYTTVAADCMARYKKLTGYDVMFLTGTDEHGQKIERKAKENGKNPQQFVDEIVASI
KELWTLLDIEYDDFIRTTEERHKSVVQKIFERFYEQGDIYKDQYEGWYCTPCESFWLERQLDEAKTCPDCAREVEWVKEE
SYFFKMSKYADRLLEHIENNPEFIQPESRKNEMINNFLNPGLEDLCVSRTTFKWGIEVPMDKDHVVYVWLDALTNYLSAL
GYLSEDDTKFQKYWPCDVHLMAKEIVRFHSIYWPIFLMALDLPLPKQVFGHGWLLLEEGKMSKSKGNVVDPEVLVNKYGS
DAIRYYLLREISFGSDGVFTLEALIRRINYDLANDLGNLLNRTVAMIDKYFDGQIPEPVVSEQVDNDLINHAQETVTETE
KHMEGMDFSEALKALWELIGRTNKYIDETQPWILGKDPNKKDRLATVLYNLSESLRYISVLLSPFMPRTPKQIRSQLGID
NNKEIQTYKSLYEWGQIPSGVRVSKDKPLFPRLEIEEELESFNDNSEEPQKEEKEQDSLKYPELKEQVDIKDFQKLDLRV
AEIVNVEPIKGADKLWKVNVDLGFENRQVVAGIKESFGAEDLIGKKVILVANLKPAKIKGVKSQGMILAANDEGTNQLEL
PNFISDKITKGSQVR

Sequences:

>Translated_655_residues
MARDNTFYITTPIYYPSDKLHIGHSYTTVAADCMARYKKLTGYDVMFLTGTDEHGQKIERKAKENGKNPQQFVDEIVASI
KELWTLLDIEYDDFIRTTEERHKSVVQKIFERFYEQGDIYKDQYEGWYCTPCESFWLERQLDEAKTCPDCAREVEWVKEE
SYFFKMSKYADRLLEHIENNPEFIQPESRKNEMINNFLNPGLEDLCVSRTTFKWGIEVPMDKDHVVYVWLDALTNYLSAL
GYLSEDDTKFQKYWPCDVHLMAKEIVRFHSIYWPIFLMALDLPLPKQVFGHGWLLLEEGKMSKSKGNVVDPEVLVNKYGS
DAIRYYLLREISFGSDGVFTLEALIRRINYDLANDLGNLLNRTVAMIDKYFDGQIPEPVVSEQVDNDLINHAQETVTETE
KHMEGMDFSEALKALWELIGRTNKYIDETQPWILGKDPNKKDRLATVLYNLSESLRYISVLLSPFMPRTPKQIRSQLGID
NNKEIQTYKSLYEWGQIPSGVRVSKDKPLFPRLEIEEELESFNDNSEEPQKEEKEQDSLKYPELKEQVDIKDFQKLDLRV
AEIVNVEPIKGADKLWKVNVDLGFENRQVVAGIKESFGAEDLIGKKVILVANLKPAKIKGVKSQGMILAANDEGTNQLEL
PNFISDKITKGSQVR
>Mature_654_residues
ARDNTFYITTPIYYPSDKLHIGHSYTTVAADCMARYKKLTGYDVMFLTGTDEHGQKIERKAKENGKNPQQFVDEIVASIK
ELWTLLDIEYDDFIRTTEERHKSVVQKIFERFYEQGDIYKDQYEGWYCTPCESFWLERQLDEAKTCPDCAREVEWVKEES
YFFKMSKYADRLLEHIENNPEFIQPESRKNEMINNFLNPGLEDLCVSRTTFKWGIEVPMDKDHVVYVWLDALTNYLSALG
YLSEDDTKFQKYWPCDVHLMAKEIVRFHSIYWPIFLMALDLPLPKQVFGHGWLLLEEGKMSKSKGNVVDPEVLVNKYGSD
AIRYYLLREISFGSDGVFTLEALIRRINYDLANDLGNLLNRTVAMIDKYFDGQIPEPVVSEQVDNDLINHAQETVTETEK
HMEGMDFSEALKALWELIGRTNKYIDETQPWILGKDPNKKDRLATVLYNLSESLRYISVLLSPFMPRTPKQIRSQLGIDN
NKEIQTYKSLYEWGQIPSGVRVSKDKPLFPRLEIEEELESFNDNSEEPQKEEKEQDSLKYPELKEQVDIKDFQKLDLRVA
EIVNVEPIKGADKLWKVNVDLGFENRQVVAGIKESFGAEDLIGKKVILVANLKPAKIKGVKSQGMILAANDEGTNQLELP
NFISDKITKGSQVR

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=546, Percent_Identity=33.3333333333333, Blast_Score=301, Evalue=1e-81,
Organism=Homo sapiens, GI14043022, Length=565, Percent_Identity=27.2566371681416, Blast_Score=194, Evalue=3e-49,
Organism=Homo sapiens, GI4507947, Length=84, Percent_Identity=42.8571428571429, Blast_Score=69, Evalue=2e-11,
Organism=Escherichia coli, GI1788432, Length=671, Percent_Identity=27.8688524590164, Blast_Score=269, Evalue=4e-73,
Organism=Escherichia coli, GI1786861, Length=175, Percent_Identity=27.4285714285714, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI1789455, Length=96, Percent_Identity=38.5416666666667, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI212645096, Length=501, Percent_Identity=32.5349301397206, Blast_Score=277, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI71992781, Length=388, Percent_Identity=31.4432989690722, Blast_Score=195, Evalue=6e-50,
Organism=Caenorhabditis elegans, GI17540794, Length=592, Percent_Identity=25.1689189189189, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI71997510, Length=177, Percent_Identity=27.683615819209, Blast_Score=69, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI71997517, Length=177, Percent_Identity=27.683615819209, Blast_Score=69, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321610, Length=534, Percent_Identity=31.8352059925094, Blast_Score=276, Evalue=1e-74,
Organism=Saccharomyces cerevisiae, GI6321704, Length=515, Percent_Identity=25.8252427184466, Blast_Score=154, Evalue=5e-38,
Organism=Saccharomyces cerevisiae, GI6323414, Length=215, Percent_Identity=26.5116279069767, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI21358483, Length=496, Percent_Identity=34.8790322580645, Blast_Score=291, Evalue=9e-79,
Organism=Drosophila melanogaster, GI24655311, Length=551, Percent_Identity=26.6787658802178, Blast_Score=181, Evalue=2e-45,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 76002; Mature: 75871

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I ; PS00287 CYSTATIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARDNTFYITTPIYYPSDKLHIGHSYTTVAADCMARYKKLTGYDVMFLTGTDEHGQKIER
CCCCCEEEEEECEECCCCCEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHH
KAKENGKNPQQFVDEIVASIKELWTLLDIEYDDFIRTTEERHKSVVQKIFERFYEQGDIY
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KDQYEGWYCTPCESFWLERQLDEAKTCPDCAREVEWVKEESYFFKMSKYADRLLEHIENN
HHHCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PEFIQPESRKNEMINNFLNPGLEDLCVSRTTFKWGIEVPMDKDHVVYVWLDALTNYLSAL
CCCCCCCHHHHHHHHHHHCCCHHHHHCCCCEEEECEECCCCCCCEEEEEHHHHHHHHHHH
GYLSEDDTKFQKYWPCDVHLMAKEIVRFHSIYWPIFLMALDLPLPKQVFGHGWLLLEEGK
CCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCC
MSKSKGNVVDPEVLVNKYGSDAIRYYLLREISFGSDGVFTLEALIRRINYDLANDLGNLL
CCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
NRTVAMIDKYFDGQIPEPVVSEQVDNDLINHAQETVTETEKHMEGMDFSEALKALWELIG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
RTNKYIDETQPWILGKDPNKKDRLATVLYNLSESLRYISVLLSPFMPRTPKQIRSQLGID
CCHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
NNKEIQTYKSLYEWGQIPSGVRVSKDKPLFPRLEIEEELESFNDNSEEPQKEEKEQDSLK
CCCHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCC
YPELKEQVDIKDFQKLDLRVAEIVNVEPIKGADKLWKVNVDLGFENRQVVAGIKESFGAE
CCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCH
DLIGKKVILVANLKPAKIKGVKSQGMILAANDEGTNQLELPNFISDKITKGSQVR
HHCCCEEEEEECCCCHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure 
ARDNTFYITTPIYYPSDKLHIGHSYTTVAADCMARYKKLTGYDVMFLTGTDEHGQKIER
CCCCEEEEEECEECCCCCEEECCCHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHH
KAKENGKNPQQFVDEIVASIKELWTLLDIEYDDFIRTTEERHKSVVQKIFERFYEQGDIY
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
KDQYEGWYCTPCESFWLERQLDEAKTCPDCAREVEWVKEESYFFKMSKYADRLLEHIENN
HHHCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PEFIQPESRKNEMINNFLNPGLEDLCVSRTTFKWGIEVPMDKDHVVYVWLDALTNYLSAL
CCCCCCCHHHHHHHHHHHCCCHHHHHCCCCEEEECEECCCCCCCEEEEEHHHHHHHHHHH
GYLSEDDTKFQKYWPCDVHLMAKEIVRFHSIYWPIFLMALDLPLPKQVFGHGWLLLEEGK
CCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCEEEEECCC
MSKSKGNVVDPEVLVNKYGSDAIRYYLLREISFGSDGVFTLEALIRRINYDLANDLGNLL
CCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCHHHHHHHHHHH
NRTVAMIDKYFDGQIPEPVVSEQVDNDLINHAQETVTETEKHMEGMDFSEALKALWELIG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHC
RTNKYIDETQPWILGKDPNKKDRLATVLYNLSESLRYISVLLSPFMPRTPKQIRSQLGID
CCHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCC
NNKEIQTYKSLYEWGQIPSGVRVSKDKPLFPRLEIEEELESFNDNSEEPQKEEKEQDSLK
CCCHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHCCC
YPELKEQVDIKDFQKLDLRVAEIVNVEPIKGADKLWKVNVDLGFENRQVVAGIKESFGAE
CCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHCCCH
DLIGKKVILVANLKPAKIKGVKSQGMILAANDEGTNQLELPNFISDKITKGSQVR
HHCCCEEEEEECCCCHHCCCCCCCCEEEEECCCCCCCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1852609 [H]