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The map label for this gene is recF

Identifier: 188584646

GI number: 188584646

Start: 3167

End: 4327

Strand: Direct

Name: recF

Synonym: Nther_0004

Alternate gene names: 188584646

Gene position: 3167-4327 (Clockwise)

Preceding gene: 188584645

Following gene: 188584647

Centisome position: 0.1

GC content: 31.7

Gene sequence:

>1161_bases
TTGAAATTAACAGAATTGTGTCTTAAAAATTTTCGCAATTACTCTAATCTAAAACTGAATTTTAAAAAGCCGATTATATT
GTTTTTTGGTGCTAATGCCCAAGGTAAAACAAACTTACTTGAAGCTATATATTATCTGGCTACAGGTAAATCCCATAGAG
CTCAAAAAGAAAAAGAATTAATCCGCTGGGAAACCAGCGGATTTTACCTAAAAGGAGAATTAGAAAAGGAACAAGCCCAA
TACACTTTAGAAATAATTACAAACTATCAGAATGGGAAAAATAAAAATTTGAAAGTTAATAATTTATCCCAAACCAATAC
AAGAAATTTCTTAAAAACCATGAATGTAGTTATTTTTAGTCCCGAAGATTTAATGCTTGTTAAAGGGACCCCAGATAATA
GGAGAAGATTTATTGATCAAGAAATAACTCAGGTGGATCCCAGTTATGACTTCTATTTGAAAAATTATTTTAAAGCTTTG
AGACAGAGAAATAAACTATTGAAAACTTATCAAGATAAAAACACCTTGGCACAACATTTACCTCCTTGGAATCAACAGTT
AGTCCATTATGGTTCTAAAATTATACTAAAAAGAGAAGAAGTTATTCATAAAATAAGATTGCTTGCCAGGTTAATTTATC
GCAAAATAACAAATCAAACTGAAAATTTAGAGCTAGATTACTCCCCTTCATTGGAATTTGAAGATTGCAAGTTTAGAGAA
CAGCTTTCGGGAGAAAAATTAGCCCATAAATTTTTAAATACTTTAAACGAAAATCTACAAAGTGATATTGAGAAAAGAAC
AACTTCCATTGGACCTCACAGGGATGATTTAATTTTTAAAATAAATAATAAAGATGCCAGGCAGTTTGGTTCCCAAGGAC
AACAACGTACTACGGTTTTGGCTCTTAAAATGGCGGAATTAGAAATGATTAAAGGAGAGAAGGGTGAATTCCCTATTTTA
CTATTAGACGATGTTTTGAGTGAATTAGATGACAATAGAAAAAAACATCTGTTAAATTTAACAGAAGGACGAGTGCAAAC
ATTCGTAACTAGCACTTCTATGGAAGATTTCAATGGTGACGTGGATATTAAAGCTAAATCCCAGGTTTTTCGAATTGATA
ACGGTGAGGCGGTGAAATTAAATGCAGGGAATCAGGAATGA

Upstream 100 bases:

>100_bases
AAAGTTATATCCAGGGGATATTGTTAACTTAAAAGACCAAGAGGAAATCATTGAAATAACTAAATGATAATATTATTTGG
ATCTCCGAAGGAGTGGTCCT

Downstream 100 bases:

>100_bases
TTTAAAAAAATTCCTTGCTAAAAGTGGTTTATTAAAGAAATTTTATCAAGAAAAAGCTATGAATTATTGGGTAGATGTCG
TAGGTGAAGAAATTGCTACC

Product: DNA replication and repair protein RecF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MKLTELCLKNFRNYSNLKLNFKKPIILFFGANAQGKTNLLEAIYYLATGKSHRAQKEKELIRWETSGFYLKGELEKEQAQ
YTLEIITNYQNGKNKNLKVNNLSQTNTRNFLKTMNVVIFSPEDLMLVKGTPDNRRRFIDQEITQVDPSYDFYLKNYFKAL
RQRNKLLKTYQDKNTLAQHLPPWNQQLVHYGSKIILKREEVIHKIRLLARLIYRKITNQTENLELDYSPSLEFEDCKFRE
QLSGEKLAHKFLNTLNENLQSDIEKRTTSIGPHRDDLIFKINNKDARQFGSQGQQRTTVLALKMAELEMIKGEKGEFPIL
LLDDVLSELDDNRKKHLLNLTEGRVQTFVTSTSMEDFNGDVDIKAKSQVFRIDNGEAVKLNAGNQE

Sequences:

>Translated_386_residues
MKLTELCLKNFRNYSNLKLNFKKPIILFFGANAQGKTNLLEAIYYLATGKSHRAQKEKELIRWETSGFYLKGELEKEQAQ
YTLEIITNYQNGKNKNLKVNNLSQTNTRNFLKTMNVVIFSPEDLMLVKGTPDNRRRFIDQEITQVDPSYDFYLKNYFKAL
RQRNKLLKTYQDKNTLAQHLPPWNQQLVHYGSKIILKREEVIHKIRLLARLIYRKITNQTENLELDYSPSLEFEDCKFRE
QLSGEKLAHKFLNTLNENLQSDIEKRTTSIGPHRDDLIFKINNKDARQFGSQGQQRTTVLALKMAELEMIKGEKGEFPIL
LLDDVLSELDDNRKKHLLNLTEGRVQTFVTSTSMEDFNGDVDIKAKSQVFRIDNGEAVKLNAGNQE
>Mature_386_residues
MKLTELCLKNFRNYSNLKLNFKKPIILFFGANAQGKTNLLEAIYYLATGKSHRAQKEKELIRWETSGFYLKGELEKEQAQ
YTLEIITNYQNGKNKNLKVNNLSQTNTRNFLKTMNVVIFSPEDLMLVKGTPDNRRRFIDQEITQVDPSYDFYLKNYFKAL
RQRNKLLKTYQDKNTLAQHLPPWNQQLVHYGSKIILKREEVIHKIRLLARLIYRKITNQTENLELDYSPSLEFEDCKFRE
QLSGEKLAHKFLNTLNENLQSDIEKRTTSIGPHRDDLIFKINNKDARQFGSQGQQRTTVLALKMAELEMIKGEKGEFPIL
LLDDVLSELDDNRKKHLLNLTEGRVQTFVTSTSMEDFNGDVDIKAKSQVFRIDNGEAVKLNAGNQE

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=377, Percent_Identity=25.9946949602122, Blast_Score=136, Evalue=3e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_NATTJ (B2A2Y9)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001916191.1
- ProteinModelPortal:   B2A2Y9
- SMR:   B2A2Y9
- GeneID:   6314824
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_0004
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 45022; Mature: 45022

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLTELCLKNFRNYSNLKLNFKKPIILFFGANAQGKTNLLEAIYYLATGKSHRAQKEKEL
CCHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
IRWETSGFYLKGELEKEQAQYTLEIITNYQNGKNKNLKVNNLSQTNTRNFLKTMNVVIFS
HEEECCCEEEEECCCHHHHHEEEEEEEECCCCCCCCEEECCCCCCHHHHHHHHCEEEEEC
PEDLMLVKGTPDNRRRFIDQEITQVDPSYDFYLKNYFKALRQRNKLLKTYQDKNTLAQHL
CCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PPWNQQLVHYGSKIILKREEVIHKIRLLARLIYRKITNQTENLELDYSPSLEFEDCKFRE
CCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHH
QLSGEKLAHKFLNTLNENLQSDIEKRTTSIGPHRDDLIFKINNKDARQFGSQGQQRTTVL
HHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHCCCCCCHHHHE
ALKMAELEMIKGEKGEFPILLLDDVLSELDDNRKKHLLNLTEGRVQTFVTSTSMEDFNGD
EEHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCHHHHHHCCCCCCEEEEEEECCHHHCCCC
VDIKAKSQVFRIDNGEAVKLNAGNQE
EEEEECCEEEEECCCCEEEECCCCCC
>Mature Secondary Structure
MKLTELCLKNFRNYSNLKLNFKKPIILFFGANAQGKTNLLEAIYYLATGKSHRAQKEKEL
CCHHHHHHHHCCCCCCEEEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
IRWETSGFYLKGELEKEQAQYTLEIITNYQNGKNKNLKVNNLSQTNTRNFLKTMNVVIFS
HEEECCCEEEEECCCHHHHHEEEEEEEECCCCCCCCEEECCCCCCHHHHHHHHCEEEEEC
PEDLMLVKGTPDNRRRFIDQEITQVDPSYDFYLKNYFKALRQRNKLLKTYQDKNTLAQHL
CCCEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PPWNQQLVHYGSKIILKREEVIHKIRLLARLIYRKITNQTENLELDYSPSLEFEDCKFRE
CCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCHHHH
QLSGEKLAHKFLNTLNENLQSDIEKRTTSIGPHRDDLIFKINNKDARQFGSQGQQRTTVL
HHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHCCCCCCHHHHE
ALKMAELEMIKGEKGEFPILLLDDVLSELDDNRKKHLLNLTEGRVQTFVTSTSMEDFNGD
EEHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCHHHHHHCCCCCCEEEEEEECCHHHCCCC
VDIKAKSQVFRIDNGEAVKLNAGNQE
EEEEECCEEEEECCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA