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The map label for this gene is aspS

Identifier: 188527535

GI number: 188527535

Start: 727832

End: 729565

Strand: Reverse

Name: aspS

Synonym: HPSH_03785

Alternate gene names: 188527535

Gene position: 729565-727832 (Counterclockwise)

Preceding gene: 188527536

Following gene: 188527534

Centisome position: 45.36

GC content: 40.6

Gene sequence:

>1734_bases
ATGCGAAGTCATTTCTGTGCAGAAATTAGTGAAAAAGATGTGGGTAAAACCATCAAAGTGGCCGGGTGGTGTAACACTTA
TAGAGACCATGGAGGCGTGGTTTTTATTGATTTAAGGGATAAAAGCGGTTTAGTGCAACTGGTCTGTGATCCTAGCTCTA
AGGCTTATGAAAAGGCTTTAGAAGTTAGGAGTGAATTTGTGCTAGTGGCTAAAGGAAAAGCGCGTTTAAGAGGGCCCGGG
TTAGAAAACCCTAAACTAAAGACCGGTAAAATTGAAATTGTTTTAGAAGAATTAGTCATTGAAAATAAAAGCGCTACCCC
ACCGATTGAAATTGGCAATAAAAATGTGAATGAAGATTTGCGCTTGAAATACCGCTATTTGGATTTGCGCTCTTTGAACG
CTTATGAGATCTTTAAATTGCGCAGCGAAGTGGCTTTAATCGCTCGTAACGCTTTAGCCCAAAAGGGCTTTTTAGAGATT
GAAACCCCCATTTTGTCTAAAACCACGCCTGAGGGGGCTAGGGATTATTTAGTGCCAAGCAGGGTGCATGAGGGCGAATT
TTTCGCGCTCCCTCAAAGCCCGCAATTGTTCAAACAGCTTTTAATGGTGGGGGGAATGGACAGGTATTTTCAAATCGCTC
GTTGCTTTAGAGATGAAGATTTGAGAGCGGACAGGCAGCCAGAATTCACGCAAATTGATGCGGAAATGAGTTTTTGTAGT
GAAAATGATGTGATGGGCGTGGTGGAAGATTTGTTGCAAGAGATTTTTAAAGCGATTGGGCATAATATTTCTAAACCTTT
TAAACGCATGCCTTATAAGGAAGCGATGGAAAATTACGGGAGCGATAAGCCGGATTTACGCTTTGAATTGCCTTTAATAG
AAGTGGGGGATTGTTTTATGGACAGCTCAAACGCTATTTTTTCTAATATCGCGCAAGATCCTAAAAACAAACGCATTAAA
GCTTTGAATGTTAAGGGGGCTGATGCGATTTTTAGCCGCAGCGTTTTAAAAGAATTAGAAGAATTTGTGTGCCAATTTGG
GGCTCAAGGCTTAGCGTATTTGCAAATTAAAGAAGATGGGGTTAAAGGGCCTTTAGTTAAATTTTTAAGCGAAAAGGGGC
TTAAGAATATTTTAGAAAAAACTGGTGCAAAAACCGGGGATATTGTCTTTTTTGGAGCCGGGGATAAAAAAATCGTGTTA
GATTACATGGGGCGTTTGCGCTTGAAGGTGGCGGAAACGCTTGATTTGATTGATAAAGACGCTTTAAATTTCTTATGGGT
AGTCAATTTCCCCATGTTTGAAAAAACCGAAAACGGCTATCATGCCGCACACCACCCTTTTACGATGCCTAAAAATATAG
AATGCAAAGATTTAGAAGAGATTGAAGCGCATGCGTATGATGTGGTGCTTAATGGCGTGGAGCTTGGTGGGGGGAGCATA
AGGATTCATAAAGAAGAAATGCAAAAAAAAGTCTTTGAAAAAATCAATATCCACGAAGAGGAAGCACAAAAGAAATTTGG
TTTTTTACTAGAAGCGCTAAAGTTTGGCGCTCCTCCTCATGGGGGCTTTGCGATAGGCTTTGATCGCTTGATCATGCTAA
TGACCAAATCCAGTAGCATTAGAGATGTGATCGCTTTCCCTAAAACGCAAAAAGCTTCATGTTTATTGACTGACGCTCCT
AGCCCCATTAATGAAGAGCAACTAAGAGAATTACACATTCGCTTAAGAAAATAA

Upstream 100 bases:

>100_bases
GATGGTTTTGTGGTAAAATCTAACATTCTTGAAATCCATGAAATGCTTAAAAAAACGCTTTCATAAATTTAATTTTTGTT
TTAATTTAAAGGGATAAAAC

Downstream 100 bases:

>100_bases
TTTGAAAGGATAAGATATGAAACAACTATTTTTGATTATTGGAGCCCCAGGGAGTGGTAAAACCACTGATGCAGAGCTTA
TCGCTAAGAATAACAGCGAA

Product: aspartyl-tRNA synthetase

Products: NA

Alternate protein names: Aspartate--tRNA ligase; AspRS [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MRSHFCAEISEKDVGKTIKVAGWCNTYRDHGGVVFIDLRDKSGLVQLVCDPSSKAYEKALEVRSEFVLVAKGKARLRGPG
LENPKLKTGKIEIVLEELVIENKSATPPIEIGNKNVNEDLRLKYRYLDLRSLNAYEIFKLRSEVALIARNALAQKGFLEI
ETPILSKTTPEGARDYLVPSRVHEGEFFALPQSPQLFKQLLMVGGMDRYFQIARCFRDEDLRADRQPEFTQIDAEMSFCS
ENDVMGVVEDLLQEIFKAIGHNISKPFKRMPYKEAMENYGSDKPDLRFELPLIEVGDCFMDSSNAIFSNIAQDPKNKRIK
ALNVKGADAIFSRSVLKELEEFVCQFGAQGLAYLQIKEDGVKGPLVKFLSEKGLKNILEKTGAKTGDIVFFGAGDKKIVL
DYMGRLRLKVAETLDLIDKDALNFLWVVNFPMFEKTENGYHAAHHPFTMPKNIECKDLEEIEAHAYDVVLNGVELGGGSI
RIHKEEMQKKVFEKINIHEEEAQKKFGFLLEALKFGAPPHGGFAIGFDRLIMLMTKSSSIRDVIAFPKTQKASCLLTDAP
SPINEEQLRELHIRLRK

Sequences:

>Translated_577_residues
MRSHFCAEISEKDVGKTIKVAGWCNTYRDHGGVVFIDLRDKSGLVQLVCDPSSKAYEKALEVRSEFVLVAKGKARLRGPG
LENPKLKTGKIEIVLEELVIENKSATPPIEIGNKNVNEDLRLKYRYLDLRSLNAYEIFKLRSEVALIARNALAQKGFLEI
ETPILSKTTPEGARDYLVPSRVHEGEFFALPQSPQLFKQLLMVGGMDRYFQIARCFRDEDLRADRQPEFTQIDAEMSFCS
ENDVMGVVEDLLQEIFKAIGHNISKPFKRMPYKEAMENYGSDKPDLRFELPLIEVGDCFMDSSNAIFSNIAQDPKNKRIK
ALNVKGADAIFSRSVLKELEEFVCQFGAQGLAYLQIKEDGVKGPLVKFLSEKGLKNILEKTGAKTGDIVFFGAGDKKIVL
DYMGRLRLKVAETLDLIDKDALNFLWVVNFPMFEKTENGYHAAHHPFTMPKNIECKDLEEIEAHAYDVVLNGVELGGGSI
RIHKEEMQKKVFEKINIHEEEAQKKFGFLLEALKFGAPPHGGFAIGFDRLIMLMTKSSSIRDVIAFPKTQKASCLLTDAP
SPINEEQLRELHIRLRK
>Mature_577_residues
MRSHFCAEISEKDVGKTIKVAGWCNTYRDHGGVVFIDLRDKSGLVQLVCDPSSKAYEKALEVRSEFVLVAKGKARLRGPG
LENPKLKTGKIEIVLEELVIENKSATPPIEIGNKNVNEDLRLKYRYLDLRSLNAYEIFKLRSEVALIARNALAQKGFLEI
ETPILSKTTPEGARDYLVPSRVHEGEFFALPQSPQLFKQLLMVGGMDRYFQIARCFRDEDLRADRQPEFTQIDAEMSFCS
ENDVMGVVEDLLQEIFKAIGHNISKPFKRMPYKEAMENYGSDKPDLRFELPLIEVGDCFMDSSNAIFSNIAQDPKNKRIK
ALNVKGADAIFSRSVLKELEEFVCQFGAQGLAYLQIKEDGVKGPLVKFLSEKGLKNILEKTGAKTGDIVFFGAGDKKIVL
DYMGRLRLKVAETLDLIDKDALNFLWVVNFPMFEKTENGYHAAHHPFTMPKNIECKDLEEIEAHAYDVVLNGVELGGGSI
RIHKEEMQKKVFEKINIHEEEAQKKFGFLLEALKFGAPPHGGFAIGFDRLIMLMTKSSSIRDVIAFPKTQKASCLLTDAP
SPINEEQLRELHIRLRK

Specific function: Unknown

COG id: COG0173

COG function: function code J; Aspartyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI40789249, Length=595, Percent_Identity=38.8235294117647, Blast_Score=401, Evalue=1e-111,
Organism=Homo sapiens, GI5031815, Length=253, Percent_Identity=30.8300395256917, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI194272210, Length=253, Percent_Identity=30.8300395256917, Blast_Score=95, Evalue=2e-19,
Organism=Homo sapiens, GI45439306, Length=177, Percent_Identity=31.638418079096, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI4758762, Length=286, Percent_Identity=27.972027972028, Blast_Score=83, Evalue=9e-16,
Organism=Homo sapiens, GI226491502, Length=256, Percent_Identity=29.6875, Blast_Score=79, Evalue=2e-14,
Organism=Escherichia coli, GI1788173, Length=588, Percent_Identity=48.1292517006803, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI1789256, Length=314, Percent_Identity=28.0254777070064, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1790571, Length=289, Percent_Identity=25.9515570934256, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI32566633, Length=593, Percent_Identity=37.2681281618887, Blast_Score=347, Evalue=9e-96,
Organism=Caenorhabditis elegans, GI71994340, Length=300, Percent_Identity=26, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=301, Percent_Identity=26.578073089701, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535927, Length=300, Percent_Identity=26, Blast_Score=87, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17551876, Length=313, Percent_Identity=25.2396166134185, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71984122, Length=295, Percent_Identity=25.7627118644068, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6325153, Length=641, Percent_Identity=33.6973478939158, Blast_Score=284, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6323011, Length=292, Percent_Identity=27.7397260273973, Blast_Score=83, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6320242, Length=294, Percent_Identity=27.5510204081633, Blast_Score=76, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321807, Length=289, Percent_Identity=25.2595155709343, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324256, Length=159, Percent_Identity=25.7861635220126, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24584738, Length=592, Percent_Identity=37.1621621621622, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24640849, Length=247, Percent_Identity=28.3400809716599, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24640851, Length=247, Percent_Identity=28.3400809716599, Blast_Score=95, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17136276, Length=274, Percent_Identity=29.5620437956204, Blast_Score=93, Evalue=6e-19,
Organism=Drosophila melanogaster, GI19921528, Length=280, Percent_Identity=27.1428571428571, Blast_Score=78, Evalue=2e-14,

Paralogues:

None

Copy number: 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR020564
- InterPro:   IPR004524
- InterPro:   IPR018153
- InterPro:   IPR002312
- InterPro:   IPR004115
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF02938 GAD; PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.12 [H]

Molecular weight: Translated: 65202; Mature: 65202

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSHFCAEISEKDVGKTIKVAGWCNTYRDHGGVVFIDLRDKSGLVQLVCDPSSKAYEKAL
CCCCHHCCCCCCCCCCEEEEEECHHHHHCCCCEEEEEEECCCCEEEEEECCCHHHHHHHH
EVRSEFVLVAKGKARLRGPGLENPKLKTGKIEIVLEELVIENKSATPPIEIGNKNVNEDL
HHHHCEEEEECCCCEECCCCCCCCCCCCCHHEEEEHHHHHCCCCCCCCCCCCCCCCCCCC
RLKYRYLDLRSLNAYEIFKLRSEVALIARNALAQKGFLEIETPILSKTTPEGARDYLVPS
EEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHCCCCC
RVHEGEFFALPQSPQLFKQLLMVGGMDRYFQIARCFRDEDLRADRQPEFTQIDAEMSFCS
CCCCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHCC
ENDVMGVVEDLLQEIFKAIGHNISKPFKRMPYKEAMENYGSDKPDLRFELPLIEVGDCFM
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHCCCCCCCEEEECCEEEECCHHC
DSSNAIFSNIAQDPKNKRIKALNVKGADAIFSRSVLKELEEFVCQFGAQGLAYLQIKEDG
CCCCHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
VKGPLVKFLSEKGLKNILEKTGAKTGDIVFFGAGDKKIVLDYMGRLRLKVAETLDLIDKD
CCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHH
ALNFLWVVNFPMFEKTENGYHAAHHPFTMPKNIECKDLEEIEAHAYDVVLNGVELGGGSI
CCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEEECEEECCCEE
RIHKEEMQKKVFEKINIHEEEAQKKFGFLLEALKFGAPPHGGFAIGFDRLIMLMTKSSSI
EEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHCCCCCH
RDVIAFPKTQKASCLLTDAPSPINEEQLRELHIRLRK
HHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRSHFCAEISEKDVGKTIKVAGWCNTYRDHGGVVFIDLRDKSGLVQLVCDPSSKAYEKAL
CCCCHHCCCCCCCCCCEEEEEECHHHHHCCCCEEEEEEECCCCEEEEEECCCHHHHHHHH
EVRSEFVLVAKGKARLRGPGLENPKLKTGKIEIVLEELVIENKSATPPIEIGNKNVNEDL
HHHHCEEEEECCCCEECCCCCCCCCCCCCHHEEEEHHHHHCCCCCCCCCCCCCCCCCCCC
RLKYRYLDLRSLNAYEIFKLRSEVALIARNALAQKGFLEIETPILSKTTPEGARDYLVPS
EEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHCCCCC
RVHEGEFFALPQSPQLFKQLLMVGGMDRYFQIARCFRDEDLRADRQPEFTQIDAEMSFCS
CCCCCCEEECCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHCC
ENDVMGVVEDLLQEIFKAIGHNISKPFKRMPYKEAMENYGSDKPDLRFELPLIEVGDCFM
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHCCCCCCCEEEECCEEEECCHHC
DSSNAIFSNIAQDPKNKRIKALNVKGADAIFSRSVLKELEEFVCQFGAQGLAYLQIKEDG
CCCCHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCC
VKGPLVKFLSEKGLKNILEKTGAKTGDIVFFGAGDKKIVLDYMGRLRLKVAETLDLIDKD
CCHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHH
ALNFLWVVNFPMFEKTENGYHAAHHPFTMPKNIECKDLEEIEAHAYDVVLNGVELGGGSI
CCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEEECEEECCCEE
RIHKEEMQKKVFEKINIHEEEAQKKFGFLLEALKFGAPPHGGFAIGFDRLIMLMTKSSSI
EEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHCCCCCH
RDVIAFPKTQKASCLLTDAPSPINEEQLRELHIRLRK
HHHHCCCCCCCCEEEEECCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA