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The map label for this gene is gltX1 [H]

Identifier: 188527509

GI number: 188527509

Start: 700499

End: 701818

Strand: Reverse

Name: gltX1 [H]

Synonym: HPSH_03645

Alternate gene names: 188527509

Gene position: 701818-700499 (Counterclockwise)

Preceding gene: 188527510

Following gene: 188527508

Centisome position: 43.63

GC content: 36.82

Gene sequence:

>1320_bases
ATGCTTCGTTTTGCGCCTTCGCCTACAGGGGATATGCATATAGGGAATTTAAGGGCAGCCATTTTTAACTACATTGTAGC
CAAACAGCAGCATAAACCCTTTCTCATTCGCATTGAAGACACGGACAAAGAGCGCAACATTGAAGGCAAAGACCAAGAAA
TTTTAGAGATTTTAAAGCTTATGGGGATAAGTTGGGACAAGCTCGTGTATCAAAGCCGTAACATAGATTATCACAGAGAA
ATGGCAGAAAAATTACTGAAAGAAAATAAAGCGTTTTATTGCTATGCGAGCACGGAGTTTTTAGAAAGAGAAAAAGAAAA
AGCCAAAAATGAAAAACGCCCTTTCAGGTATTTAGACGAGTGGGCGGCTTTAGAAAAAGACAAGCACCATGCCCCTGTGG
TGCGTTTAAAAGCCCCAAATCATGCGGTGTCTTTCAACGATGCGATTAAAAAAGAAGTGGAATTTGAGCCTTATGAGTTG
GATTCTTTTGTGCTTTTAAGGAAGGATAAAAGCCCTACTTATAATTTCGCTTGCGCATGCGATGATTTGCTCTATGAAAT
CAGTCTGATTATTAGAGGCGAAGATCATGTGAGTAACACCCCTAAACAAATTCTAATCCAGCAAGCTTTAGGCTCAAACA
ATCCGATTGTTTATGCGCATTTACCCATTATTTTAGACGAAACAAGCGGTAAAAAGATGAGTAAAAGAGATGAAGTCTCT
AGCGTGAAATGGCTTTTGAATCAAGGGTTTTTACCGGAGGCTATTGTGAATTACCTCATCACTATCGGTAATAAAGTGCC
TAAGGAAGTTTTCAGCCTTGATGAAGCGATAGAATGGTTTGATTTAGAAAATCTTTCCAGTTCCCCGGCTCATTTTAATT
TAAAATATTTGAAACACTTAAACCATGAGCATTTAAAGCTTTTAGACGATGAAAAGTTATTAGAACTCATTTTAATAAAA
GATAAAAATCTCTTAGGGCTTTTAAGATTGTTTATAGAAGAATGCGGCACGCTTTTAGAATTGAAAGAAAAAATTTCGTT
GTTTTTAGAGCCAAAAGATATTGTTAAAACTTATGAAAACGAAGATTTTAAAGAGCGTTGTTTAGTGCTTTTTAACGCTC
TAAAAAGCATGGATTTTCAAGCGTATAAGGATTTTGAAAGTTTTAAAAAAGAAGCCATGCGATTAAGCCAGCTTAAGGGT
AAGGATTTTTTCAAACCTTTGCGCATCCTTTTAACCGGGAACTCGCATGGCGTTGAATTGCCTTTGATTTTCCCCTATAT
CCAAAGCCATCATCAAAAAGTTTTAAGGCTCAAAGCATGA

Upstream 100 bases:

>100_bases
TAAATAATGCTTGTTCATGTTTGATAAAAACAGAATTACCACAGCATAATTAAGTATTCTTTAGGTATAATCAAAAACTA
TTTTTCAATAAAGGGTTTTT

Downstream 100 bases:

>100_bases
TATTTTCCACTCTTATTAATGCGGTAGCGGTGATTTTAAGCTCGCTCATTACGATCTATATGTGGGTAGTGATCATTTAT
TCGCTTATTAGCTTTGTGCA

Product: glutamylglutaminyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 1; GluRS 1 [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MLRFAPSPTGDMHIGNLRAAIFNYIVAKQQHKPFLIRIEDTDKERNIEGKDQEILEILKLMGISWDKLVYQSRNIDYHRE
MAEKLLKENKAFYCYASTEFLEREKEKAKNEKRPFRYLDEWAALEKDKHHAPVVRLKAPNHAVSFNDAIKKEVEFEPYEL
DSFVLLRKDKSPTYNFACACDDLLYEISLIIRGEDHVSNTPKQILIQQALGSNNPIVYAHLPIILDETSGKKMSKRDEVS
SVKWLLNQGFLPEAIVNYLITIGNKVPKEVFSLDEAIEWFDLENLSSSPAHFNLKYLKHLNHEHLKLLDDEKLLELILIK
DKNLLGLLRLFIEECGTLLELKEKISLFLEPKDIVKTYENEDFKERCLVLFNALKSMDFQAYKDFESFKKEAMRLSQLKG
KDFFKPLRILLTGNSHGVELPLIFPYIQSHHQKVLRLKA

Sequences:

>Translated_439_residues
MLRFAPSPTGDMHIGNLRAAIFNYIVAKQQHKPFLIRIEDTDKERNIEGKDQEILEILKLMGISWDKLVYQSRNIDYHRE
MAEKLLKENKAFYCYASTEFLEREKEKAKNEKRPFRYLDEWAALEKDKHHAPVVRLKAPNHAVSFNDAIKKEVEFEPYEL
DSFVLLRKDKSPTYNFACACDDLLYEISLIIRGEDHVSNTPKQILIQQALGSNNPIVYAHLPIILDETSGKKMSKRDEVS
SVKWLLNQGFLPEAIVNYLITIGNKVPKEVFSLDEAIEWFDLENLSSSPAHFNLKYLKHLNHEHLKLLDDEKLLELILIK
DKNLLGLLRLFIEECGTLLELKEKISLFLEPKDIVKTYENEDFKERCLVLFNALKSMDFQAYKDFESFKKEAMRLSQLKG
KDFFKPLRILLTGNSHGVELPLIFPYIQSHHQKVLRLKA
>Mature_439_residues
MLRFAPSPTGDMHIGNLRAAIFNYIVAKQQHKPFLIRIEDTDKERNIEGKDQEILEILKLMGISWDKLVYQSRNIDYHRE
MAEKLLKENKAFYCYASTEFLEREKEKAKNEKRPFRYLDEWAALEKDKHHAPVVRLKAPNHAVSFNDAIKKEVEFEPYEL
DSFVLLRKDKSPTYNFACACDDLLYEISLIIRGEDHVSNTPKQILIQQALGSNNPIVYAHLPIILDETSGKKMSKRDEVS
SVKWLLNQGFLPEAIVNYLITIGNKVPKEVFSLDEAIEWFDLENLSSSPAHFNLKYLKHLNHEHLKLLDDEKLLELILIK
DKNLLGLLRLFIEECGTLLELKEKISLFLEPKDIVKTYENEDFKERCLVLFNALKSMDFQAYKDFESFKKEAMRLSQLKG
KDFFKPLRILLTGNSHGVELPLIFPYIQSHHQKVLRLKA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=361, Percent_Identity=31.0249307479224, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI62241042, Length=261, Percent_Identity=24.5210727969349, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1788743, Length=436, Percent_Identity=35.3211009174312, Blast_Score=227, Evalue=1e-60,
Organism=Escherichia coli, GI226510925, Length=245, Percent_Identity=29.3877551020408, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71993394, Length=306, Percent_Identity=33.3333333333333, Blast_Score=142, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17511007, Length=257, Percent_Identity=24.124513618677, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324540, Length=340, Percent_Identity=34.1176470588235, Blast_Score=171, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6321192, Length=123, Percent_Identity=32.520325203252, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24665321, Length=323, Percent_Identity=28.7925696594427, Blast_Score=146, Evalue=3e-35,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 51266; Mature: 51266

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRFAPSPTGDMHIGNLRAAIFNYIVAKQQHKPFLIRIEDTDKERNIEGKDQEILEILKL
CCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
MGISWDKLVYQSRNIDYHREMAEKLLKENKAFYCYASTEFLEREKEKAKNEKRPFRYLDE
HCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCCHHHHHH
WAALEKDKHHAPVVRLKAPNHAVSFNDAIKKEVEFEPYELDSFVLLRKDKSPTYNFACAC
HHHHCCCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCEECCCEEEEECCCCCCCEEEEHH
DDLLYEISLIIRGEDHVSNTPKQILIQQALGSNNPIVYAHLPIILDETSGKKMSKRDEVS
HHHHHEEEEEEECCHHCCCCHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCHHHHHH
SVKWLLNQGFLPEAIVNYLITIGNKVPKEVFSLDEAIEWFDLENLSSSPAHFNLKYLKHL
HHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHC
NHEHLKLLDDEKLLELILIKDKNLLGLLRLFIEECGTLLELKEKISLFLEPKDIVKTYEN
CHHHHHHCCHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHCC
EDFKERCLVLFNALKSMDFQAYKDFESFKKEAMRLSQLKGKDFFKPLRILLTGNSHGVEL
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEEECCCCCEEE
PLIFPYIQSHHQKVLRLKA
EEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLRFAPSPTGDMHIGNLRAAIFNYIVAKQQHKPFLIRIEDTDKERNIEGKDQEILEILKL
CCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHH
MGISWDKLVYQSRNIDYHREMAEKLLKENKAFYCYASTEFLEREKEKAKNEKRPFRYLDE
HCCCHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHCCCCCHHHHHH
WAALEKDKHHAPVVRLKAPNHAVSFNDAIKKEVEFEPYELDSFVLLRKDKSPTYNFACAC
HHHHCCCCCCCCEEEEECCCCCCCCCHHHHHCCCCCCEECCCEEEEECCCCCCCEEEEHH
DDLLYEISLIIRGEDHVSNTPKQILIQQALGSNNPIVYAHLPIILDETSGKKMSKRDEVS
HHHHHEEEEEEECCHHCCCCHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCHHHHHH
SVKWLLNQGFLPEAIVNYLITIGNKVPKEVFSLDEAIEWFDLENLSSSPAHFNLKYLKHL
HHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHHC
NHEHLKLLDDEKLLELILIKDKNLLGLLRLFIEECGTLLELKEKISLFLEPKDIVKTYEN
CHHHHHHCCHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHEEECCHHHHHHHCC
EDFKERCLVLFNALKSMDFQAYKDFESFKKEAMRLSQLKGKDFFKPLRILLTGNSHGVEL
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHEEEEEECCCCCEEE
PLIFPYIQSHHQKVLRLKA
EEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA