Click here to switch to the map view.

The map label for this gene is aspA

Identifier: 188527503

GI number: 188527503

Start: 694549

End: 695955

Strand: Reverse

Name: aspA

Synonym: HPSH_03615

Alternate gene names: 188527503

Gene position: 695955-694549 (Counterclockwise)

Preceding gene: 188527504

Following gene: 188527502

Centisome position: 43.27

GC content: 43.0

Gene sequence:

>1407_bases
ATGCGTATTGAGCATGATTTCATTGGGCAAATGGAAATTAGCGATGAAGTTTATTACGGGATCCAAACTTTAAGAGCGAG
TGAAAATTTTTTCATCACCAACGACAAGCTTTGCAGTTATCCTGTTTTTATTAAATCTTTTGCTCAAGTCAAAAAAGCGG
CTGCTTTAGCGAACGCGCAATTAGGCTTGATTGATGAAAAGCTTAAGATTGCGATTTGCCATGCGTGCGATTTGTTGATT
GATGGCAAATACCATGATCAATTCATTGTGGATATGATTCAAGGGGGGGCTGGCACAAGCACGAACATGAACATGAATGA
AGTGATTGCTAATTTGGCTTTAGAATACATGGGGCATAAAAAGGGCGAGTATCAATTTTGCCACCCAAACGATCATGTCA
ATCGCTCTCAATCCACCAATGATGCCTATCCTAGTGCGTTAAAAATCGCTATTTATGAGCGCTTGAGTAATTTAGTCGCT
CCCATGAAGGCTTTAAGGGACGCTTTCGCTCAAAAAGCTAAGGAATTCGCTCATGTGATTAAAATGGGGCGCACCCAGCT
TCAAGACGCTGTGCCTATGACTTTAGGCCAAGAGTTTGAAACTTACGCTTTAATGGTTGATAGGGATATTGAGCAGGTTT
TAGACGCTAGGAATTGGGTAAGAGAGCTTAATTTAGGCGGAACGGCTATTGGCACAGGGATCAATTCGCACCCGGATTAT
CGCAGTTTGATTGAAAAGAAAATCCAAGAAGTAACGGGCCGCCCCTTTGTCATGGCTAATAACTTGATAGAAGCCACTCA
AAGCACGGGGGCGTATGTGCAAGTGAGTGGGGTGTTAAAGCGTATTGCAGTGAAACTCTCTAAAGTTTGTAACGATCTTA
GGTTACTCAGCTCAGGCCCTAGAGCCGGACTGAATGAAATCAATTTGCCTAAAATGCAGCCGGGCAGCTCTATTATGCCC
GGTAAAGTCAATCCGGTGATCCCTGAAGTGGTCAATCAAGTGTGCTTTGCGGTGATTGGGAATGATTTGAGCGTGGCGTT
AGCCGCAGAAGGAGGGCAATTGCAACTCAATGTGTTTGAGCCGGTTATCGCTTACAAGCTTTTCCATTCCTTTGTGATTT
TAGGGCGCGCGATTGAAACCTTAACGACTAAATGCGTGGAAGGCATCACGGCTAATGAAAAGATTTGCCACGATTATGTC
TTTAACAGCATTGGCATAGTTACTGCGCTAAACCCTCATATCGGCTATGAAAAATCCGCTATGATCGCTAAAGAAGCCTT
AAAAAGCGATCGCTCTATCTATGACATCGCTTTAGAAAAGAAAATCTTAACTAAAGAGCAACTGGACGATATTTTCAAGC
CAGAAAACATGCTAAGCCCTCACGCTTTCAAAAAGCATAAAGACTGA

Upstream 100 bases:

>100_bases
AATCAACGCCTTAGGGGCAAAAGTTGCACGCTTAAAAGAAAAATAACTCAATATTTGGTTACAATAGCTAGAATATTTTT
TAACGATACAAGGAGCTTTT

Downstream 100 bases:

>100_bases
ACGCTTTGCAACTTTCACGCCTTCAAACTTTACGCTCTCTTTATATGGAGCGTCTTTTGGGCGAAACTTACACCAATATC
AACCTTACCAAGCCCCAAAA

Product: aspartate ammonia-lyase

Products: NA

Alternate protein names: Aspartase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MRIEHDFIGQMEISDEVYYGIQTLRASENFFITNDKLCSYPVFIKSFAQVKKAAALANAQLGLIDEKLKIAICHACDLLI
DGKYHDQFIVDMIQGGAGTSTNMNMNEVIANLALEYMGHKKGEYQFCHPNDHVNRSQSTNDAYPSALKIAIYERLSNLVA
PMKALRDAFAQKAKEFAHVIKMGRTQLQDAVPMTLGQEFETYALMVDRDIEQVLDARNWVRELNLGGTAIGTGINSHPDY
RSLIEKKIQEVTGRPFVMANNLIEATQSTGAYVQVSGVLKRIAVKLSKVCNDLRLLSSGPRAGLNEINLPKMQPGSSIMP
GKVNPVIPEVVNQVCFAVIGNDLSVALAAEGGQLQLNVFEPVIAYKLFHSFVILGRAIETLTTKCVEGITANEKICHDYV
FNSIGIVTALNPHIGYEKSAMIAKEALKSDRSIYDIALEKKILTKEQLDDIFKPENMLSPHAFKKHKD

Sequences:

>Translated_468_residues
MRIEHDFIGQMEISDEVYYGIQTLRASENFFITNDKLCSYPVFIKSFAQVKKAAALANAQLGLIDEKLKIAICHACDLLI
DGKYHDQFIVDMIQGGAGTSTNMNMNEVIANLALEYMGHKKGEYQFCHPNDHVNRSQSTNDAYPSALKIAIYERLSNLVA
PMKALRDAFAQKAKEFAHVIKMGRTQLQDAVPMTLGQEFETYALMVDRDIEQVLDARNWVRELNLGGTAIGTGINSHPDY
RSLIEKKIQEVTGRPFVMANNLIEATQSTGAYVQVSGVLKRIAVKLSKVCNDLRLLSSGPRAGLNEINLPKMQPGSSIMP
GKVNPVIPEVVNQVCFAVIGNDLSVALAAEGGQLQLNVFEPVIAYKLFHSFVILGRAIETLTTKCVEGITANEKICHDYV
FNSIGIVTALNPHIGYEKSAMIAKEALKSDRSIYDIALEKKILTKEQLDDIFKPENMLSPHAFKKHKD
>Mature_468_residues
MRIEHDFIGQMEISDEVYYGIQTLRASENFFITNDKLCSYPVFIKSFAQVKKAAALANAQLGLIDEKLKIAICHACDLLI
DGKYHDQFIVDMIQGGAGTSTNMNMNEVIANLALEYMGHKKGEYQFCHPNDHVNRSQSTNDAYPSALKIAIYERLSNLVA
PMKALRDAFAQKAKEFAHVIKMGRTQLQDAVPMTLGQEFETYALMVDRDIEQVLDARNWVRELNLGGTAIGTGINSHPDY
RSLIEKKIQEVTGRPFVMANNLIEATQSTGAYVQVSGVLKRIAVKLSKVCNDLRLLSSGPRAGLNEINLPKMQPGSSIMP
GKVNPVIPEVVNQVCFAVIGNDLSVALAAEGGQLQLNVFEPVIAYKLFHSFVILGRAIETLTTKCVEGITANEKICHDYV
FNSIGIVTALNPHIGYEKSAMIAKEALKSDRSIYDIALEKKILTKEQLDDIFKPENMLSPHAFKKHKD

Specific function: Unknown

COG id: COG1027

COG function: function code E; Aspartate ammonia-lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family. Aspartase subfamily [H]

Homologues:

Organism=Homo sapiens, GI19743875, Length=461, Percent_Identity=44.0347071583514, Blast_Score=376, Evalue=1e-104,
Organism=Escherichia coli, GI87082375, Length=470, Percent_Identity=55.531914893617, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1787896, Length=459, Percent_Identity=39.6514161220044, Blast_Score=356, Evalue=2e-99,
Organism=Caenorhabditis elegans, GI17553882, Length=462, Percent_Identity=44.1558441558442, Blast_Score=385, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI32565146, Length=326, Percent_Identity=43.8650306748466, Blast_Score=278, Evalue=5e-75,
Organism=Saccharomyces cerevisiae, GI6324993, Length=461, Percent_Identity=41.648590021692, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24640177, Length=461, Percent_Identity=43.8177874186551, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640179, Length=461, Percent_Identity=43.8177874186551, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI78710009, Length=463, Percent_Identity=43.6285097192225, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24662684, Length=462, Percent_Identity=43.5064935064935, Blast_Score=367, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24583245, Length=462, Percent_Identity=37.4458874458874, Blast_Score=315, Evalue=5e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004708
- InterPro:   IPR003031
- InterPro:   IPR018951
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761 [H]

Pfam domain/function: PF10415 FumaraseC_C; PF00206 Lyase_1 [H]

EC number: =4.3.1.1 [H]

Molecular weight: Translated: 51924; Mature: 51924

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIEHDFIGQMEISDEVYYGIQTLRASENFFITNDKLCSYPVFIKSFAQVKKAAALANAQ
CCCCCCCCCCEECCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCH
LGLIDEKLKIAICHACDLLIDGKYHDQFIVDMIQGGAGTSTNMNMNEVIANLALEYMGHK
HCCCCHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC
KGEYQFCHPNDHVNRSQSTNDAYPSALKIAIYERLSNLVAPMKALRDAFAQKAKEFAHVI
CCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMGRTQLQDAVPMTLGQEFETYALMVDRDIEQVLDARNWVRELNLGGTAIGTGINSHPDY
HHHHHHHHHHCCHHHCCCHHHHHEEHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHH
RSLIEKKIQEVTGRPFVMANNLIEATQSTGAYVQVSGVLKRIAVKLSKVCNDLRLLSSGP
HHHHHHHHHHHCCCCEEEECHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
RAGLNEINLPKMQPGSSIMPGKVNPVIPEVVNQVCFAVIGNDLSVALAAEGGQLQLNVFE
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEHHH
PVIAYKLFHSFVILGRAIETLTTKCVEGITANEKICHDYVFNSIGIVTALNPHIGYEKSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHH
MIAKEALKSDRSIYDIALEKKILTKEQLDDIFKPENMLSPHAFKKHKD
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC
>Mature Secondary Structure
MRIEHDFIGQMEISDEVYYGIQTLRASENFFITNDKLCSYPVFIKSFAQVKKAAALANAQ
CCCCCCCCCCEECCHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCH
LGLIDEKLKIAICHACDLLIDGKYHDQFIVDMIQGGAGTSTNMNMNEVIANLALEYMGHK
HCCCCHHHHHHHHHHHHHEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCC
KGEYQFCHPNDHVNRSQSTNDAYPSALKIAIYERLSNLVAPMKALRDAFAQKAKEFAHVI
CCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KMGRTQLQDAVPMTLGQEFETYALMVDRDIEQVLDARNWVRELNLGGTAIGTGINSHPDY
HHHHHHHHHHCCHHHCCCHHHHHEEHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHH
RSLIEKKIQEVTGRPFVMANNLIEATQSTGAYVQVSGVLKRIAVKLSKVCNDLRLLSSGP
HHHHHHHHHHHCCCCEEEECHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCC
RAGLNEINLPKMQPGSSIMPGKVNPVIPEVVNQVCFAVIGNDLSVALAAEGGQLQLNVFE
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEHHH
PVIAYKLFHSFVILGRAIETLTTKCVEGITANEKICHDYVFNSIGIVTALNPHIGYEKSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEECCCCCCCHHH
MIAKEALKSDRSIYDIALEKKILTKEQLDDIFKPENMLSPHAFKKHKD
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]