Click here to switch to the map view.

The map label for this gene is cofH [H]

Identifier: 188527495

GI number: 188527495

Start: 686183

End: 687244

Strand: Reverse

Name: cofH [H]

Synonym: HPSH_03575

Alternate gene names: 188527495

Gene position: 687244-686183 (Counterclockwise)

Preceding gene: 188527496

Following gene: 188527494

Centisome position: 42.72

GC content: 40.96

Gene sequence:

>1062_bases
ATGCGCATTAACAGAGAAGAAATTTTAGATCTAATGAAAAACGCGCCCTTGAAAGAATTAGGGCAAAAGGCTTTAGAAAT
CAAGCAACGCTTGCACCCTGAAAACTTGACGACTTTTATTGTGGATAGGAATATCAATTACACCAATATTTGTTTTGTGG
ATTGCAAGTTTTGCGCTTTCAAACGCACCCTAAAAGAAAAAGACGCCTATGTGTTGAGCTATGAAGAAATTGATCAAAAG
ATTGAAGAATTACTCGCTATTGGCGGCACGCAGATCCTTTTTCAAGGGGGGGTGCACCCGCAGCTCAAAATAGACTATTA
TGAAAATTTAGTCAGCCATATCGCTCAAAAATTCCCCACCATTACCATTCATGGTTTTAGCGCGGTTGAAATTGATTACA
TTTCTAAAATCTCTAAATTGTCTTTAAAAGAAGTTTTAGAAAGGTTGAAAAACGCCGGTTTAAGCTCCATTCCAGGAGCG
GGAGCAGAAATATTAAGCGATAGGGTGCGCGATGTGATCGCTCCTAAAAAATTGAGCAGTGATCGCTGGATTGAAGTGCA
TAGAATGGCGCATCTTTGTGGGATTAAAAGCACGGCTACCATGATGTTTGGGAGCGTGGATAATGAAGAAGATGTGGTGG
AGCATTTGCAAAGAGTGCGCGATTTGCAAGATGAAACCGGTGGCTTTAGAGCTTTCATTTTATGGAGTTTTCAGCCCGAC
AACACCCCCTTAAAAGAAGAAATCCCAAGCATTAAAAAAGCGAGTTCCAATCGGTATTTACGCTATTTGGCATGCAGTAG
GATTTTTTTAGATAACATTCAAAACATACAGAGCTCATGGGTTACTCAAGGCTCTATGATAGGGCAGTTAGCCTTATTGT
TTGGAGCGAATGATTTAGGGAGTGTGATGATGGAAGAAAATGTAGTGAAAGCGGCCGGAACGAGTTTTTGCATGAATGAA
GCGGAAATGATAGAGCTTATTGAAGACATTGGGAGCGTGGCGGCTAAACGAAACACCGCTTATGAAATCTTAAAGCGTTA
TCCGGCTAAAGCAAAGGCATGA

Upstream 100 bases:

>100_bases
GGCGATGGCAATGGCAAGAAATGTAAAGGGCTATGCTTCAACCCTAACATGAACGATTACACGCAACACTTTGAATTTTC
TATGGGAACAAGGTTTTAAA

Downstream 100 bases:

>100_bases
GAATGAAAAAAATTTTAATCACTTTATTATTAGGAGTTTTTATGGGGTTACAAGCGAGCACTTTGACACACCAAGAAATC
AATCAAGCTAAAGTCCCTGT

Product: hypothetical protein

Products: NA

Alternate protein names: 7,8-didemethyl-8-hydroxy-5-deazariboflavin synthase subunit 2 [H]

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MRINREEILDLMKNAPLKELGQKALEIKQRLHPENLTTFIVDRNINYTNICFVDCKFCAFKRTLKEKDAYVLSYEEIDQK
IEELLAIGGTQILFQGGVHPQLKIDYYENLVSHIAQKFPTITIHGFSAVEIDYISKISKLSLKEVLERLKNAGLSSIPGA
GAEILSDRVRDVIAPKKLSSDRWIEVHRMAHLCGIKSTATMMFGSVDNEEDVVEHLQRVRDLQDETGGFRAFILWSFQPD
NTPLKEEIPSIKKASSNRYLRYLACSRIFLDNIQNIQSSWVTQGSMIGQLALLFGANDLGSVMMEENVVKAAGTSFCMNE
AEMIELIEDIGSVAAKRNTAYEILKRYPAKAKA

Sequences:

>Translated_353_residues
MRINREEILDLMKNAPLKELGQKALEIKQRLHPENLTTFIVDRNINYTNICFVDCKFCAFKRTLKEKDAYVLSYEEIDQK
IEELLAIGGTQILFQGGVHPQLKIDYYENLVSHIAQKFPTITIHGFSAVEIDYISKISKLSLKEVLERLKNAGLSSIPGA
GAEILSDRVRDVIAPKKLSSDRWIEVHRMAHLCGIKSTATMMFGSVDNEEDVVEHLQRVRDLQDETGGFRAFILWSFQPD
NTPLKEEIPSIKKASSNRYLRYLACSRIFLDNIQNIQSSWVTQGSMIGQLALLFGANDLGSVMMEENVVKAAGTSFCMNE
AEMIELIEDIGSVAAKRNTAYEILKRYPAKAKA
>Mature_353_residues
MRINREEILDLMKNAPLKELGQKALEIKQRLHPENLTTFIVDRNINYTNICFVDCKFCAFKRTLKEKDAYVLSYEEIDQK
IEELLAIGGTQILFQGGVHPQLKIDYYENLVSHIAQKFPTITIHGFSAVEIDYISKISKLSLKEVLERLKNAGLSSIPGA
GAEILSDRVRDVIAPKKLSSDRWIEVHRMAHLCGIKSTATMMFGSVDNEEDVVEHLQRVRDLQDETGGFRAFILWSFQPD
NTPLKEEIPSIKKASSNRYLRYLACSRIFLDNIQNIQSSWVTQGSMIGQLALLFGANDLGSVMMEENVVKAAGTSFCMNE
AEMIELIEDIGSVAAKRNTAYEILKRYPAKAKA

Specific function: Catalyzes the radical-mediated transfer of the hydroxybenzyl group from 4-hydroxyphenylpyruvate (HPP) to 5-amino- 6-ribitylamino-2,4(1H,3H)-pyrimidinedione to form 7,8-didemethyl- 8-hydroxy-5-deazariboflavin (FO) [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. CofH family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR019940
- InterPro:   IPR006638
- InterPro:   IPR020050
- InterPro:   IPR005244
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 39912; Mature: 39912

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRINREEILDLMKNAPLKELGQKALEIKQRLHPENLTTFIVDRNINYTNICFVDCKFCAF
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEEHHHHHH
KRTLKEKDAYVLSYEEIDQKIEELLAIGGTQILFQGGVHPQLKIDYYENLVSHIAQKFPT
HHHHHHCCCEEEEHHHHHHHHHHHHHCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHCCE
ITIHGFSAVEIDYISKISKLSLKEVLERLKNAGLSSIPGAGAEILSDRVRDVIAPKKLSS
EEEECCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
DRWIEVHRMAHLCGIKSTATMMFGSVDNEEDVVEHLQRVRDLQDETGGFRAFILWSFQPD
CCHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCC
NTPLKEEIPSIKKASSNRYLRYLACSRIFLDNIQNIQSSWVTQGSMIGQLALLFGANDLG
CCCHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHH
SVMMEENVVKAAGTSFCMNEAEMIELIEDIGSVAAKRNTAYEILKRYPAKAKA
HHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRINREEILDLMKNAPLKELGQKALEIKQRLHPENLTTFIVDRNINYTNICFVDCKFCAF
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEEEEHHHHHH
KRTLKEKDAYVLSYEEIDQKIEELLAIGGTQILFQGGVHPQLKIDYYENLVSHIAQKFPT
HHHHHHCCCEEEEHHHHHHHHHHHHHCCCCEEEEECCCCCCEEHHHHHHHHHHHHHHCCE
ITIHGFSAVEIDYISKISKLSLKEVLERLKNAGLSSIPGAGAEILSDRVRDVIAPKKLSS
EEEECCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCC
DRWIEVHRMAHLCGIKSTATMMFGSVDNEEDVVEHLQRVRDLQDETGGFRAFILWSFQPD
CCHHHHHHHHHHHCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCC
NTPLKEEIPSIKKASSNRYLRYLACSRIFLDNIQNIQSSWVTQGSMIGQLALLFGANDLG
CCCHHHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHH
SVMMEENVVKAAGTSFCMNEAEMIELIEDIGSVAAKRNTAYEILKRYPAKAKA
HHHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]