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The map label for this gene is rnhA

Identifier: 188527490

GI number: 188527490

Start: 680700

End: 681164

Strand: Reverse

Name: rnhA

Synonym: HPSH_03550

Alternate gene names: 188527490

Gene position: 681164-680700 (Counterclockwise)

Preceding gene: 188527491

Following gene: 188527489

Centisome position: 42.35

GC content: 40.43

Gene sequence:

>465_bases
ATGCAAGAAATTGAAATTTTTTGCGATGGCTCTTCTTTAGGCAATCCCGGGCCAGGCGGTTATGCGGCGATTTTACGCTA
TAAAGATAAAGAAAAAATCATCAGTGGAGGCGAATCATTCACCACGAATAACCGCATGGAATTAAGAGCGCTCAATGAAG
CGTTAAAAATTTTGAAACGCCCATGCCATATCACGCTTTATAGCGATTCGCAATACGTGTGCCAAGCGATCAATGTGTGG
CTAACTAACTGGCAAAAAAAGAATTTTTCTAAAGTCAAAAATGTGGATTTATGGAAAGAATTTTTAGAAGTCTCTAAAGG
GCATTTGATCATGGCTGTTTGGATCAAGGGGCATAATGGGCATGCCGAGAATGAACGATGCGATAGCCTCGCTAAATTAG
AGGCACAAAAACGGGTAAAAACGACCAAAGGGAAAAACGATGAAAAACAAACGCTCTCAAAATAG

Upstream 100 bases:

>100_bases
ATCTTTCCTTTTGAAAGCCATAGGTGTCCTGTGTGTTACCAGTTAGCGTTTATGGATATGGTGCTTAAAATTTCTAAAGA
AAGCTATGGGAGTGGATTGG

Downstream 100 bases:

>100_bases
CCCTTATATAACGAATAGCCCTTATGCAACGCTAGAAAAAGCTTTGGGGTATTCTTTTAAAGACAAGCGTTTATTGGAGC
AAGCCTTAACGCATAAATCA

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MQEIEIFCDGSSLGNPGPGGYAAILRYKDKEKIISGGESFTTNNRMELRALNEALKILKRPCHITLYSDSQYVCQAINVW
LTNWQKKNFSKVKNVDLWKEFLEVSKGHLIMAVWIKGHNGHAENERCDSLAKLEAQKRVKTTKGKNDEKQTLSK

Sequences:

>Translated_154_residues
MQEIEIFCDGSSLGNPGPGGYAAILRYKDKEKIISGGESFTTNNRMELRALNEALKILKRPCHITLYSDSQYVCQAINVW
LTNWQKKNFSKVKNVDLWKEFLEVSKGHLIMAVWIKGHNGHAENERCDSLAKLEAQKRVKTTKGKNDEKQTLSK
>Mature_154_residues
MQEIEIFCDGSSLGNPGPGGYAAILRYKDKEKIISGGESFTTNNRMELRALNEALKILKRPCHITLYSDSQYVCQAINVW
LTNWQKKNFSKVKNVDLWKEFLEVSKGHLIMAVWIKGHNGHAENERCDSLAKLEAQKRVKTTKGKNDEKQTLSK

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Escherichia coli, GI1786408, Length=140, Percent_Identity=52.8571428571429, Blast_Score=151, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6323890, Length=166, Percent_Identity=30.1204819277108, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 17542; Mature: 17542

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEIEIFCDGSSLGNPGPGGYAAILRYKDKEKIISGGESFTTNNRMELRALNEALKILKR
CCCEEEEECCCCCCCCCCCCEEEEEEECCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
PCHITLYSDSQYVCQAINVWLTNWQKKNFSKVKNVDLWKEFLEVSKGHLIMAVWIKGHNG
CCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHCCCCEEEEEEEECCCC
HAENERCDSLAKLEAQKRVKTTKGKNDEKQTLSK
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCC
>Mature Secondary Structure
MQEIEIFCDGSSLGNPGPGGYAAILRYKDKEKIISGGESFTTNNRMELRALNEALKILKR
CCCEEEEECCCCCCCCCCCCEEEEEEECCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCC
PCHITLYSDSQYVCQAINVWLTNWQKKNFSKVKNVDLWKEFLEVSKGHLIMAVWIKGHNG
CCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHCCHHHHHHHHHHCCCCEEEEEEEECCCC
HAENERCDSLAKLEAQKRVKTTKGKNDEKQTLSK
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA