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The map label for this gene is mraW

Identifier: 188527446

GI number: 188527446

Start: 628472

End: 629398

Strand: Reverse

Name: mraW

Synonym: HPSH_03320

Alternate gene names: 188527446

Gene position: 629398-628472 (Counterclockwise)

Preceding gene: 188527447

Following gene: 188527445

Centisome position: 39.13

GC content: 38.83

Gene sequence:

>927_bases
TTGCAAGAAATAGAGAGTTTGCACCAAAGTGTTTTGTTGCAAGAAGTTTTGCAAGCGTTTGCACCTTTAGAAGAAGGGGT
TTTGATAGATTGCACTTTAGGGTTAGGGGGGCATTCTAAAGCGATTCTATCCCAAAAACCGCACCTAAAACTCATTGGCA
TTGATAAGGATAAATTCGCTCAAGAAATCGCTAAAGAACGATTGAAAGCCTTTGAAGGGCGTTATAATCTCTTAAGTGGG
GGGTTTGCCAAACGCTTTAAAGAAGCCCTAGAAATGCATGACAAGGAGATTAAAGGGGTTTTAGTGGATTTAGGGGTGAG
CTCTTTGCAGCTTGATGATGATAGCAGAGGGTTTAATTTCCACTCGCACGCTCTGGATATGCGCATGGATTTAGAGAGCG
ATTTGAACGCTCAAAAAGTCATCAACTCTTATCCTGTAGTGGCGTTAGAAAAAATCTTTAAAGACTATGGCGAAATCAAA
GAATACAAAAAAATCGCCCACAAAATTGCAGAAAGGCGCGCTAAAAAACCCTTTAAAGACGCTAAGGATTTGAGCGATTT
TTTAAGCTCCCTTTCTAAAAATAAAAAAATCCATCCAGCGACTTTGGTGTTTCAAGCCGTTCGCATAGAAGTCAATAGTG
AATTAGAAGAATTAAAAGAGTTTTTACAATGCGCTAGAAACCTTAAGGAAGCGATTTTGTGCGTGATTTCTTTCCATTCT
TTAGAAGACGCGTTAGTGAAAAACGCTTTTAAAGATTACGCTAAAAATTGCATTTGCGATCCTTCAAGTTTTAAATGCAC
TTGCTCTAACAATCATGCTTTAGGCGAAATTTTAACCAAAAAGCCCATCACTCCAAGCCCAGAAGAAATTAAAAACAACA
GGCGTTCACGAAGCGCTAAAATGAGGGTGTTTCAATTCAAACCATGA

Upstream 100 bases:

>100_bases
GTCGATCATAAAGAAACTTTTAAGCGTAACCAACAATACCAAAACCAATACCATTGAGTAGGCATTTGGAAAATTCATCT
AAAGGTTTTAAGGGGGTTGT

Downstream 100 bases:

>100_bases
ATAAGTCAATGAGTAAGCCGTGAGTATAAAAAGCCATTCTTCAAATGAAAAAGAACGCTTTGTGCGCATAGAAGAAGACG
AAAAGAAAGAATTGTTTGCT

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MQEIESLHQSVLLQEVLQAFAPLEEGVLIDCTLGLGGHSKAILSQKPHLKLIGIDKDKFAQEIAKERLKAFEGRYNLLSG
GFAKRFKEALEMHDKEIKGVLVDLGVSSLQLDDDSRGFNFHSHALDMRMDLESDLNAQKVINSYPVVALEKIFKDYGEIK
EYKKIAHKIAERRAKKPFKDAKDLSDFLSSLSKNKKIHPATLVFQAVRIEVNSELEELKEFLQCARNLKEAILCVISFHS
LEDALVKNAFKDYAKNCICDPSSFKCTCSNNHALGEILTKKPITPSPEEIKNNRRSRSAKMRVFQFKP

Sequences:

>Translated_308_residues
MQEIESLHQSVLLQEVLQAFAPLEEGVLIDCTLGLGGHSKAILSQKPHLKLIGIDKDKFAQEIAKERLKAFEGRYNLLSG
GFAKRFKEALEMHDKEIKGVLVDLGVSSLQLDDDSRGFNFHSHALDMRMDLESDLNAQKVINSYPVVALEKIFKDYGEIK
EYKKIAHKIAERRAKKPFKDAKDLSDFLSSLSKNKKIHPATLVFQAVRIEVNSELEELKEFLQCARNLKEAILCVISFHS
LEDALVKNAFKDYAKNCICDPSSFKCTCSNNHALGEILTKKPITPSPEEIKNNRRSRSAKMRVFQFKP
>Mature_308_residues
MQEIESLHQSVLLQEVLQAFAPLEEGVLIDCTLGLGGHSKAILSQKPHLKLIGIDKDKFAQEIAKERLKAFEGRYNLLSG
GFAKRFKEALEMHDKEIKGVLVDLGVSSLQLDDDSRGFNFHSHALDMRMDLESDLNAQKVINSYPVVALEKIFKDYGEIK
EYKKIAHKIAERRAKKPFKDAKDLSDFLSSLSKNKKIHPATLVFQAVRIEVNSELEELKEFLQCARNLKEAILCVISFHS
LEDALVKNAFKDYAKNCICDPSSFKCTCSNNHALGEILTKKPITPSPEEIKNNRRSRSAKMRVFQFKP

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=339, Percent_Identity=31.858407079646, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI165377202, Length=196, Percent_Identity=34.1836734693878, Blast_Score=101, Evalue=9e-22,
Organism=Escherichia coli, GI1786270, Length=306, Percent_Identity=36.2745098039216, Blast_Score=167, Evalue=5e-43,
Organism=Drosophila melanogaster, GI62472493, Length=328, Percent_Identity=32.3170731707317, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI28571637, Length=328, Percent_Identity=32.3170731707317, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 34890; Mature: 34890

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQEIESLHQSVLLQEVLQAFAPLEEGVLIDCTLGLGGHSKAILSQKPHLKLIGIDKDKFA
CHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHCCCCCEEEEECCHHHHH
QEIAKERLKAFEGRYNLLSGGFAKRFKEALEMHDKEIKGVLVDLGVSSLQLDDDSRGFNF
HHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
HSHALDMRMDLESDLNAQKVINSYPVVALEKIFKDYGEIKEYKKIAHKIAERRAKKPFKD
HHHHHHHHHCCHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AKDLSDFLSSLSKNKKIHPATLVFQAVRIEVNSELEELKEFLQCARNLKEAILCVISFHS
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEDALVKNAFKDYAKNCICDPSSFKCTCSNNHALGEILTKKPITPSPEEIKNNRRSRSAK
HHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHH
MRVFQFKP
EEEEEECC
>Mature Secondary Structure
MQEIESLHQSVLLQEVLQAFAPLEEGVLIDCTLGLGGHSKAILSQKPHLKLIGIDKDKFA
CHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHCCCCCEEEEECCHHHHH
QEIAKERLKAFEGRYNLLSGGFAKRFKEALEMHDKEIKGVLVDLGVSSLQLDDDSRGFNF
HHHHHHHHHHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
HSHALDMRMDLESDLNAQKVINSYPVVALEKIFKDYGEIKEYKKIAHKIAERRAKKPFKD
HHHHHHHHHCCHHCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AKDLSDFLSSLSKNKKIHPATLVFQAVRIEVNSELEELKEFLQCARNLKEAILCVISFHS
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEDALVKNAFKDYAKNCICDPSSFKCTCSNNHALGEILTKKPITPSPEEIKNNRRSRSAK
HHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHH
MRVFQFKP
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA