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The map label for this gene is rffA [H]

Identifier: 187935545

GI number: 187935545

Start: 3395293

End: 3396414

Strand: Reverse

Name: rffA [H]

Synonym: CLL_A3243

Alternate gene names: 187935545

Gene position: 3396414-3395293 (Counterclockwise)

Preceding gene: 187934897

Following gene: 187932605

Centisome position: 89.37

GC content: 29.32

Gene sequence:

>1122_bases
ATGATAAATTTTAATGAACCTCCTTTCACAGGAAAGGAATTAGACTATATAAAACAAGCTATAGAAAATAAGAAAATATG
TGGCGATGGTGAATTTACAAAAAATTGTAATGGATGGATAGAAGAGACTAGTAGAACCAAGAAAGCATTATTAACTACAT
CGTGTACACATGCACTTGAAATGGCAGCTATTTTGACAGATATTAAAGAAGGTGATGAGGTAATTATGCCATCATATACT
TTTGTATCAACAGCAGATGCATTTGTTATGAGAGGTGCTAAAATAGTATTTGTTGATATTAGACCAGATACTATGAATAT
TGATGAAACTTTAATAGAAGATGCAATAACAAGTAAAACAAAAGTTATAGTACCAGTTCATTATGCAGGAGTTGCATGCG
AGATGGACAAAATAATGGAACTTGCAAAAAAACATAAGTTATATGTTGTTGAAGATGCAGCGCAAGGTGTTATGTCAGAA
TATAAAAGTAGAGAACTTGGTAGTATTGGCGATTTTGGTTGTTATAGTTTTCATGAAACTAAGAATTATAGTATGGGCGA
AGGTGGTGCAATTTTAATACAAAATGAAGATTTTATTGAAAGAGCAGAAATAATAAGGGAAAAAGGAACTGATAGAAGTA
AGTTCTTTAGGGGACAAGTTGATAAATATACTTGGGTTGAAACGGGTTCATCTTATTTACCAAGTGAACTAAATGCAGCT
TACCTTTGGGCACAGTTGGAAATGGCTGATGATATAAATAATAGAAGATTAGAAATATGGAATAGATATTATGAGGGATT
AAAAGAAATACATGAAATAGATCTTCCAGTTATTCCAGAAGAATGTAAACATAATGCACATATGTTTTACATAAAGACAG
AAGATTTAGAAGAAAGAATAAATCTTATAAAATATTTAAAGCAAAATGAAATACAAACAGTATTTCATTATATACCATTA
CATAGTTCCACTAATGGTATGAAAGTTGGAAGATTTAATGGACAAGATAAGTATACAACTAGAGAAAGTGAAAGGTTATT
AAGATTACCTTTATATTATGGATTAGATGATACAGAAGTAGATTATATAATTAAAAAAATAAAGTTATTTTATAAAAGGT
AA

Upstream 100 bases:

>100_bases
ATAATAAAAAATTCATATCTACAGGAAAGTATTTAGAAATAGATTAAATAAGATAAATTTAAATAGTTTCCTAGTTTGAA
ATTATAAAGGATGAGGAATA

Downstream 100 bases:

>100_bases
TGTGAGGATTAAAAATATGGAAATATCAGTTGTTATACCGGTATATGGTTGTAAGGGAGCAGTATTTGAGCTTCATGAAA
GATTAGTTGGATCGTTAGAA

Product: TDP-4-oxo-6-deoxy-D-glucose transaminase

Products: TDP-D-fucosamine; 2-oxoglutarate [C]

Alternate protein names: NA

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MINFNEPPFTGKELDYIKQAIENKKICGDGEFTKNCNGWIEETSRTKKALLTTSCTHALEMAAILTDIKEGDEVIMPSYT
FVSTADAFVMRGAKIVFVDIRPDTMNIDETLIEDAITSKTKVIVPVHYAGVACEMDKIMELAKKHKLYVVEDAAQGVMSE
YKSRELGSIGDFGCYSFHETKNYSMGEGGAILIQNEDFIERAEIIREKGTDRSKFFRGQVDKYTWVETGSSYLPSELNAA
YLWAQLEMADDINNRRLEIWNRYYEGLKEIHEIDLPVIPEECKHNAHMFYIKTEDLEERINLIKYLKQNEIQTVFHYIPL
HSSTNGMKVGRFNGQDKYTTRESERLLRLPLYYGLDDTEVDYIIKKIKLFYKR

Sequences:

>Translated_373_residues
MINFNEPPFTGKELDYIKQAIENKKICGDGEFTKNCNGWIEETSRTKKALLTTSCTHALEMAAILTDIKEGDEVIMPSYT
FVSTADAFVMRGAKIVFVDIRPDTMNIDETLIEDAITSKTKVIVPVHYAGVACEMDKIMELAKKHKLYVVEDAAQGVMSE
YKSRELGSIGDFGCYSFHETKNYSMGEGGAILIQNEDFIERAEIIREKGTDRSKFFRGQVDKYTWVETGSSYLPSELNAA
YLWAQLEMADDINNRRLEIWNRYYEGLKEIHEIDLPVIPEECKHNAHMFYIKTEDLEERINLIKYLKQNEIQTVFHYIPL
HSSTNGMKVGRFNGQDKYTTRESERLLRLPLYYGLDDTEVDYIIKKIKLFYKR
>Mature_373_residues
MINFNEPPFTGKELDYIKQAIENKKICGDGEFTKNCNGWIEETSRTKKALLTTSCTHALEMAAILTDIKEGDEVIMPSYT
FVSTADAFVMRGAKIVFVDIRPDTMNIDETLIEDAITSKTKVIVPVHYAGVACEMDKIMELAKKHKLYVVEDAAQGVMSE
YKSRELGSIGDFGCYSFHETKNYSMGEGGAILIQNEDFIERAEIIREKGTDRSKFFRGQVDKYTWVETGSSYLPSELNAA
YLWAQLEMADDINNRRLEIWNRYYEGLKEIHEIDLPVIPEECKHNAHMFYIKTEDLEERINLIKYLKQNEIQTVFHYIPL
HSSTNGMKVGRFNGQDKYTTRESERLLRLPLYYGLDDTEVDYIIKKIKLFYKR

Specific function: Involved in ECA elongation [H]

COG id: COG0399

COG function: function code M; Predicted pyridoxal phosphate-dependent enzyme apparently involved in regulation of cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the degT/dnrJ/eryC1 family [H]

Homologues:

Organism=Escherichia coli, GI2367285, Length=376, Percent_Identity=64.3617021276596, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI145693159, Length=376, Percent_Identity=29.2553191489362, Blast_Score=158, Evalue=5e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000653
- InterPro:   IPR012749
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01041 DegT_DnrJ_EryC1 [H]

EC number: NA

Molecular weight: Translated: 43122; Mature: 43122

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINFNEPPFTGKELDYIKQAIENKKICGDGEFTKNCNGWIEETSRTKKALLTTSCTHALE
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MAAILTDIKEGDEVIMPSYTFVSTADAFVMRGAKIVFVDIRPDTMNIDETLIEDAITSKT
HHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCC
KVIVPVHYAGVACEMDKIMELAKKHKLYVVEDAAQGVMSEYKSRELGSIGDFGCYSFHET
EEEEEEECCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCEECCCC
KNYSMGEGGAILIQNEDFIERAEIIREKGTDRSKFFRGQVDKYTWVETGSSYLPSELNAA
CCCCCCCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCE
YLWAQLEMADDINNRRLEIWNRYYEGLKEIHEIDLPVIPEECKHNAHMFYIKTEDLEERI
EEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEEECCCHHHHH
NLIKYLKQNEIQTVFHYIPLHSSTNGMKVGRFNGQDKYTTRESERLLRLPLYYGLDDTEV
HHHHHHHHCCHHHHHHEEECCCCCCCEEEECCCCCCCCCCCHHHHEEEEHEEECCCCCHH
DYIIKKIKLFYKR
HHHHHHHHHHHCC
>Mature Secondary Structure
MINFNEPPFTGKELDYIKQAIENKKICGDGEFTKNCNGWIEETSRTKKALLTTSCTHALE
CCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MAAILTDIKEGDEVIMPSYTFVSTADAFVMRGAKIVFVDIRPDTMNIDETLIEDAITSKT
HHHHHHHHCCCCCEEECCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCCC
KVIVPVHYAGVACEMDKIMELAKKHKLYVVEDAAQGVMSEYKSRELGSIGDFGCYSFHET
EEEEEEECCCHHHHHHHHHHHHHHCCEEEEEHHHHHHHHHHHHCCCCCCCCCCCEECCCC
KNYSMGEGGAILIQNEDFIERAEIIREKGTDRSKFFRGQVDKYTWVETGSSYLPSELNAA
CCCCCCCCCEEEEECCHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEECCCCCCCCCCCCE
YLWAQLEMADDINNRRLEIWNRYYEGLKEIHEIDLPVIPEECKHNAHMFYIKTEDLEERI
EEEEEEEHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEEECCCHHHHH
NLIKYLKQNEIQTVFHYIPLHSSTNGMKVGRFNGQDKYTTRESERLLRLPLYYGLDDTEV
HHHHHHHHCCHHHHHHEEECCCCCCCEEEECCCCCCCCCCCHHHHEEEEHEEECCCCCHH
DYIIKKIKLFYKR
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-4-dehydro-6-deoxy-D-glucose; L-glutamate [C]

Specific reaction: dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate = TDP-D-fucosamine + 2-oxoglutarate [C]

General reaction: Carbon-Oxygen Lyases; Hydro-Lyases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1379743; 9278503; 8366065 [H]