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The map label for this gene is folC [H]

Identifier: 187935191

GI number: 187935191

Start: 2595987

End: 2597300

Strand: Reverse

Name: folC [H]

Synonym: CLL_A2503

Alternate gene names: 187935191

Gene position: 2597300-2595987 (Counterclockwise)

Preceding gene: 187934393

Following gene: 187933346

Centisome position: 68.34

GC content: 27.25

Gene sequence:

>1314_bases
ATGAAGATGAATTATGAAGAAGCAATGAATTATATAAATAGTGTTGGAAGATTTGGATCTAACTATGGTCTTAAAAGGAC
TTATAGATTATTAGAATTACTAGGAGAACCTCAAAAAAAATTAAAATTAGTGCATATAGCTGGAACTAATGGTAAGGGCT
CTACAACTTCAATGATAACAGCTATTTTAATGGGAATGGGTTACAAGGTAGGTATGTATACTTCCCCATATTTAGAGGAA
TTTGAAGAAAGAATACAAATTAATGGTAAAAATATTCCTAAAAATAAATTAACTGAATTAATTAATATTGTAAAAGAAGC
TGTAGATAAAGTTATAAAAGAAGGCTATGAACACCCAACTGAATTTGAGATTTTAACAACTGTTATGTATTTGTATTATT
ATAATGAAAAAGTTGATTATGCCATAATTGAAGTTGGACTTGGTGGAGAGTTAGATTCTACAAATGTAATAACTCCAATT
GTTAGTGTTATTACATCAATTAGCTTTGATCATATGAATATTTTGGGGAATTGTTTATATGAAATTGCTATGCAAAAAGC
AGGAATAATAAAAGAAGAAATTCCAGTAGTAGTTTATCCTCAAACTAATGAAGCCATGAGAGCTATAGTAGAAAAGGCTA
AAATAACAAAGAGCACAGTATACGAAATAGATGGTAATAGTGGAACATTTATAGAGGTAAAGCACGAAGAAAAATTTTAT
CAAAAAGTTAAAATTTATACTGGCAAGGAATTATATAATATTAATTTACCATTAATGGGAGAACATCAAATTTTAAATTT
AAGTGTAGCTTTAACTACAATAGAGATATTATGTGAAAGAGAGGGTTTAATATTTAATAAAGAGTTAATTGAAAATTCAA
TTGAATCTGTAAAGTGGAAAGGTCGATTGGAAGTATTAAATAAAAAACCATTAATTGTAATGGATGGAGCACACAATATA
GAAGGAATAAAGATGCTAAAAAATAATGTGAAAAAATATTTTAAGTACAATAATGTTTTCTTATTATTAGGAATTTTAGC
AGATAAGCAAGTAGAAGAAATGATCAAGGAAATAACACCAATGGCATATAAGGTTTATGCATTAACACCTCATAGTGATA
GGGCCGAATTAAATACAGAATTGAAAGATCATATATTAAAGTATAATAAAAATACCATAGCTTTAGACAGTTATGAAGAC
GCAATGAAAACCGTGTTATCTGAAGCAACTTCAGAAGATTTAATATTGATTAGTGGTTCATTATATATGATAGGAGATAT
GAGAAAAATAATAACGCAAAAAATAATGATATAG

Upstream 100 bases:

>100_bases
GCATATATCAGCATATATTAAAAAATGTGAAACAAATATTAATACAGTTAATTATATAAGCTAAATAAGCAGATTTAATA
GATAATTAGAGGGGAAGCAA

Downstream 100 bases:

>100_bases
CATTATCATATCTATTTTTTTGTATTTGAAGGGAATGAGAATTTAAATGTTTGATTCTAATGATATATGTGCATATGAAA
AAAAATCCATATACGAAGAA

Product: folylpolyglutamate synthase

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MKMNYEEAMNYINSVGRFGSNYGLKRTYRLLELLGEPQKKLKLVHIAGTNGKGSTTSMITAILMGMGYKVGMYTSPYLEE
FEERIQINGKNIPKNKLTELINIVKEAVDKVIKEGYEHPTEFEILTTVMYLYYYNEKVDYAIIEVGLGGELDSTNVITPI
VSVITSISFDHMNILGNCLYEIAMQKAGIIKEEIPVVVYPQTNEAMRAIVEKAKITKSTVYEIDGNSGTFIEVKHEEKFY
QKVKIYTGKELYNINLPLMGEHQILNLSVALTTIEILCEREGLIFNKELIENSIESVKWKGRLEVLNKKPLIVMDGAHNI
EGIKMLKNNVKKYFKYNNVFLLLGILADKQVEEMIKEITPMAYKVYALTPHSDRAELNTELKDHILKYNKNTIALDSYED
AMKTVLSEATSEDLILISGSLYMIGDMRKIITQKIMI

Sequences:

>Translated_437_residues
MKMNYEEAMNYINSVGRFGSNYGLKRTYRLLELLGEPQKKLKLVHIAGTNGKGSTTSMITAILMGMGYKVGMYTSPYLEE
FEERIQINGKNIPKNKLTELINIVKEAVDKVIKEGYEHPTEFEILTTVMYLYYYNEKVDYAIIEVGLGGELDSTNVITPI
VSVITSISFDHMNILGNCLYEIAMQKAGIIKEEIPVVVYPQTNEAMRAIVEKAKITKSTVYEIDGNSGTFIEVKHEEKFY
QKVKIYTGKELYNINLPLMGEHQILNLSVALTTIEILCEREGLIFNKELIENSIESVKWKGRLEVLNKKPLIVMDGAHNI
EGIKMLKNNVKKYFKYNNVFLLLGILADKQVEEMIKEITPMAYKVYALTPHSDRAELNTELKDHILKYNKNTIALDSYED
AMKTVLSEATSEDLILISGSLYMIGDMRKIITQKIMI
>Mature_437_residues
MKMNYEEAMNYINSVGRFGSNYGLKRTYRLLELLGEPQKKLKLVHIAGTNGKGSTTSMITAILMGMGYKVGMYTSPYLEE
FEERIQINGKNIPKNKLTELINIVKEAVDKVIKEGYEHPTEFEILTTVMYLYYYNEKVDYAIIEVGLGGELDSTNVITPI
VSVITSISFDHMNILGNCLYEIAMQKAGIIKEEIPVVVYPQTNEAMRAIVEKAKITKSTVYEIDGNSGTFIEVKHEEKFY
QKVKIYTGKELYNINLPLMGEHQILNLSVALTTIEILCEREGLIFNKELIENSIESVKWKGRLEVLNKKPLIVMDGAHNI
EGIKMLKNNVKKYFKYNNVFLLLGILADKQVEEMIKEITPMAYKVYALTPHSDRAELNTELKDHILKYNKNTIALDSYED
AMKTVLSEATSEDLILISGSLYMIGDMRKIITQKIMI

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=365, Percent_Identity=26.5753424657534, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI66932990, Length=365, Percent_Identity=26.5753424657534, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI1788654, Length=414, Percent_Identity=27.0531400966184, Blast_Score=167, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17553150, Length=455, Percent_Identity=27.9120879120879, Blast_Score=171, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI71984923, Length=455, Percent_Identity=28.3516483516484, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17553148, Length=455, Percent_Identity=27.9120879120879, Blast_Score=170, Evalue=2e-42,
Organism=Saccharomyces cerevisiae, GI6323760, Length=436, Percent_Identity=32.5688073394495, Blast_Score=189, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6324815, Length=357, Percent_Identity=31.6526610644258, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6322718, Length=308, Percent_Identity=27.5974025974026, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24641571, Length=319, Percent_Identity=28.8401253918495, Blast_Score=135, Evalue=6e-32,
Organism=Drosophila melanogaster, GI24581568, Length=195, Percent_Identity=30.2564102564103, Blast_Score=92, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 49802; Mature: 49802

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS01012 FOLYLPOLYGLU_SYNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMNYEEAMNYINSVGRFGSNYGLKRTYRLLELLGEPQKKLKLVHIAGTNGKGSTTSMIT
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHEEEEEEECCCCCCCHHHHHH
AILMGMGYKVGMYTSPYLEEFEERIQINGKNIPKNKLTELINIVKEAVDKVIKEGYEHPT
HHHHHCCCEECCCCCHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
EFEILTTVMYLYYYNEKVDYAIIEVGLGGELDSTNVITPIVSVITSISFDHMNILGNCLY
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
EIAMQKAGIIKEEIPVVVYPQTNEAMRAIVEKAKITKSTVYEIDGNSGTFIEVKHEEKFY
HHHHHHCCCHHHCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECHHHHH
QKVKIYTGKELYNINLPLMGEHQILNLSVALTTIEILCEREGLIFNKELIENSIESVKWK
HHHEEEECCEEEEECCCCCCCCEEEEEHHHHHHHHHHHCCCCCEECHHHHHHHHHHCCCC
GRLEVLNKKPLIVMDGAHNIEGIKMLKNNVKKYFKYNNVFLLLGILADKQVEEMIKEITP
CHHEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEEEHHCCHHHHHHHHHHCC
MAYKVYALTPHSDRAELNTELKDHILKYNKNTIALDSYEDAMKTVLSEATSEDLILISGS
CEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCEEEEECC
LYMIGDMRKIITQKIMI
EEEEHHHHHHHHHHHCC
>Mature Secondary Structure
MKMNYEEAMNYINSVGRFGSNYGLKRTYRLLELLGEPQKKLKLVHIAGTNGKGSTTSMIT
CCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHEEEEEEECCCCCCCHHHHHH
AILMGMGYKVGMYTSPYLEEFEERIQINGKNIPKNKLTELINIVKEAVDKVIKEGYEHPT
HHHHHCCCEECCCCCHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
EFEILTTVMYLYYYNEKVDYAIIEVGLGGELDSTNVITPIVSVITSISFDHMNILGNCLY
HHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHH
EIAMQKAGIIKEEIPVVVYPQTNEAMRAIVEKAKITKSTVYEIDGNSGTFIEVKHEEKFY
HHHHHHCCCHHHCCCEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECHHHHH
QKVKIYTGKELYNINLPLMGEHQILNLSVALTTIEILCEREGLIFNKELIENSIESVKWK
HHHEEEECCEEEEECCCCCCCCEEEEEHHHHHHHHHHHCCCCCEECHHHHHHHHHHCCCC
GRLEVLNKKPLIVMDGAHNIEGIKMLKNNVKKYFKYNNVFLLLGILADKQVEEMIKEITP
CHHEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCEEEEEEHHCCHHHHHHHHHHCC
MAYKVYALTPHSDRAELNTELKDHILKYNKNTIALDSYEDAMKTVLSEATSEDLILISGS
CEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCEEEEECC
LYMIGDMRKIITQKIMI
EEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]